DIP-STR: Highly Sensitive Markers for the Analysis of Unbalanced Genomic Mixtures.

Samples containing highly unbalanced DNA mixtures from two individuals commonly occur both in forensic mixed stains and in peripheral blood DNA microchimerism induced by pregnancy or following organ transplant. Because of PCR amplification bias, the genetic identification of a DNA that contributes trace amounts to a mixed sample represents a tremendous challenge. This means that standard genetic markers, namely microsatellites, also referred as short tandem repeats (STR), and single-nucleotide polymorphism (SNP) have limited power in addressing common questions of forensic and medical genetics. To address this issue, we developed a molecular marker, named DIP-STR that relies on pairing deletion-insertion polymorphisms (DIP) with STR. This novel analytical approach allows for the unambiguous genotyping of a minor component in the presence of a major component, where DIP-STR genotypes of the minor were successfully procured at ratios up to 1:1,000. The compound nature of this marker generates a high level of polymorphism that is suitable for identity testing. Here, we demonstrate the power of the DIP-STR approach on an initial set of nine markers surveyed in a Swiss population. Finally, we discuss the limitations and potential applications of our new system including preliminary tests on clinical samples and estimates of their performance on simulated DNA mixtures

Finite temperature mobility of a particle coupled to a fermion environment

We study numerically the finite temperature and frequency mobility of a particle coupled by a local interaction to a system of spinless fermions in one dimension. We find that when the model is integrable (particle mass equal to the mass of fermions) the static mobility diverges. Further, an enhanced mobility is observed over a finite parameter range away from the integrable point. We present a novel analysis of the finite temperature static mobility based on a random matrix theory description of the many-body Hamiltonian.Comment: 11 pages (RevTeX), 5 Postscript files, compressed using uufile

Evidence for ideal insulating/conducting state in a 1D integrable system

Using numerical diagonalization techniques we analyze the finite temperature/frequency conductance of a one dimensional model of interacting spinless fermions. Depending on the interaction, the observed finite temperature charge stiffness and low frequency conductance indicate a fundamental difference between integrable and non-integrable cases. The integrable systems behave as ideal conductors in the metallic regime and as ideal insulators in the insulating one. The non-integrable systems are, as expected, generic conductors in the metallic regime and activated ones in the insulating regime.Comment: revtex file, followed by 5 uuencoded postscript figure

Coherent Control for a Two-level System Coupled to Phonons

The interband polarizations induced by two phase-locked pulses in a semiconductor show strong interference effects depending on the time tau_1 separating the pulses. The four-wave mixing signal diffracted from a third pulse delayed by tau is coherently controlled by tuning tau_1. The four-wave mixing response is evaluated exactly for a two-level system coupled to a single LO phonon. In the weak coupling regime it shows oscillations with the phonon frequency which turn into sharp peaks at multiples of the phonon period for a larger coupling strength. Destructive interferences between the two phase-locked pulses produce a splitting of the phonon peaks into a doublet. For fixed tau but varying tau_1 the signal shows rapid oscillations at the interband-transition frequency, whose amplitude exhibits bursts at multiples of the phonon period.Comment: 4 pages, 4 figures, RevTex, content change

Time evolution of a quantum many-body system: transition from integrability to ergodicity in thermodynamic limit

Numerical evidence is given for non-ergodic (non-mixing) behavior, exhibiting ideal transport, of a simple non-integrable many-body quantum system in the thermodynamic limit, namely kicked $t-V$ model of spinless fermions on a ring. However, for sufficiently large kick parameters $t$ and $V$ we recover quantum ergodicity, and normal transport, which can be described by random matrix theory.Comment: 4 pages in RevTex (6 figures in PostScript included

Rotating optical catheter tip for optical coherence tomography

The present invention relates to a rotating catheter tip for optical coherence tomography based on the use of an optical fiber that does not rotate, that is enclosed in a catheter, which has a tip rotates under the influence of a fluid drive system to redirect light from the fiber to a surrounding vessel and the light reflected or backscattered from the vessel back to the optical fiber.Board of Regents, University of Texas Syste

Quantum Transport in a Nanosize Silicon-on-Insulator Metal-Oxide-Semiconductor

An approach is developed for the determination of the current flowing through a nanosize silicon-on-insulator (SOI) metal-oxide-semiconductor field-effect transistors (MOSFET). The quantum mechanical features of the electron transport are extracted from the numerical solution of the quantum Liouville equation in the Wigner function representation. Accounting for electron scattering due to ionized impurities, acoustic phonons and surface roughness at the Si/SiO2 interface, device characteristics are obtained as a function of a channel length. From the Wigner function distributions, the coexistence of the diffusive and the ballistic transport naturally emerges. It is shown that the scattering mechanisms tend to reduce the ballistic component of the transport. The ballistic component increases with decreasing the channel length.Comment: 21 pages, 8 figures, E-mail addresses: [email protected]

Some considerations on the derivation of the nonlinear quantum Boltzmann equation

Abstract. In this paper we analyze a system of N identical quantum particles in a weakcoupling regime. The time evolution of the Wigner transform of the one-particle reduced density matrix is represented by means of a perturbative series. The expansion is obtained upon iterating the Duhamel formula. For short times, we rigorously prove that a subseries of the latter, converges to the solution of the Boltzmann equation which is physically relevant in the context. In particular, we recover the transition rate as it is predicted by Fermi's Golden Rule. However, we are not able to prove that the quantity neglected while retaining a subseries of the complete original perturbative expansion, indeed vanishes in the limit: we only give plausibility arguments in this direction. The present study holds in any space dimension d ≥ 2

Fanconi anemia protein FANCD2 inhibits TRF1 polyADP-ribosylation through tankyrase1-dependent manner

Background: Fanconi anemia (FA) is a rare autosomal recessive syndrome characterized by developmental abnormalities, progressive bone marrow failure, and predisposition to cancer. The key FA protein FANCD2 crosstalks with members of DNA damage and repair pathways that also play a role at telomeres. Therefore, we investigated whether FANCD2 has a similar involvement at telomeres. Results: We reveal that FANCD2 may perform a novel function separate to the FANCD2/BRCA pathway. This function includes FANCD2 interaction with one of the telomere components, the PARP family member tankyrase-1. Moreover, FANCD2 inhibits tankyrase-1 activity in vitro. In turn, FANCD2 deficiency increases the polyADP-ribosylation of telomere binding factor TRF1. Conclusions: FANCD2 binding and inhibiting tankyrase-1PARsylation at telomeres may provide an additional step within the FA pathway for the regulation of genomic integrity