Pure shift amide detection in conventional and TROSY-type experiments of 13^{13}C,15^{15}N-labeled proteins

Large coupling networks in uniformly $^{13}$C,$^{15}$N-labeled biomolecules induce broad multiplets that even in flexible proteins are frequently not recognized as such. The reason is that given multiplets typically consist of a large number of individual resonances that result in a single broad line, in which individual components are no longer resolved. We here introduce a real-time pure shift acquisition scheme for the detection of amide protons which is based on 13C-BIRDr,X. As a result the full homo- and heteronuclear coupling network can be suppressed at low power leading to real singlets at substantially improved resolution and uncompromised sensitivity. The method is tested on a small globular and an intrinsically disordered protein (IDP) where the average spectral resolution is increased by a factor of ~ 2 and higher. Equally important, the approach works without saturation of water magnetization for solvent suppression and exchanging amide protons are not affected by saturation transfer

Outline analysis of the grapevine (Vitis vinifera L.) berry shape by elliptic Fourier descriptors

Grapevine berry morphology is one of the most important features in table grape production. In this study, berry samples of 46 grapevine accessions were investigated for 3 consecutive years with elliptic Fourier descriptors (EFD) to evaluate shape diversity. Ten reference shapes obtained from the OIV descriptor list were involved and principal component (PC) scores summarizing the EFD's were statistically evaluated with Two way ANOVA and discriminant analysis. The cummulative contribution of the five principal components was 96.83 %. Two way ANOVA revealed that berry shape had high variability within the accessions and years. Based on the linear discriminant analysis, reference shapes were compared to those of the accessions and graphic reconstruction was carried out. OIV references were considered as unknown samples and grouped into the accession classes. Overall correct classification of the accessions into their group was 13.88 %. Our results showed that EFD together with reference shapes are a powerful method to discribe berry shape and possibly give the future basis of uvometric evaluation of grapevine cultivars

Ampelometric evaluation of wild grape (Vitis vinifera L. ssp. sylvestris (C.C. Gmel.) Hegi) accessions in the germplasm collection of FEM-IASMA, Italy

In this paper, 45 wild grapevine accessions collected during two consecutive years were compared for 36 ampelometric traits using digital image analysis. The sample set contained male and female individuals from different geographic regions: Germany, North Italy, Central Italy, South Italy, Sardinia and Turkey. The leaf morphological data from the collected samples suggest that geographic origin, gender and vintage could have an effect on ampelometric traits in this species

Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes

Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phase and is subsequently maintained across mitotic divisions. Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restricted chromatin assembly at centromeres is unique to CENP-A nucleosomes and does not involve assembly of other H3 variants. Strikingly, stable retention is restricted to the CENP-A/H4 core of the nucleosome, which we find to outlast general chromatin across several cell divisions. We further show that cell cycle timing of CENP-A assembly is independent of centromeric DNA sequences and instead is mediated by the CENP-A targeting domain. Unexpectedly, this domain also induces stable transmission of centromeric nucleosomes, independent of the CENP-A deposition factor HJURP. This demonstrates that intrinsic properties of the CENP-A protein direct its cell cycle-restricted assembly and induces quantitative mitotic transmission of the CENP-A/H4 nucleosome core, ensuring long-term stability and epigenetic maintenance of centromere position.FCT fellpwships: (SFRH/BD/74284/2010, SFRH/BPD/69115/2010), National Institutes of Health grant: (GM082989), Burroughs Wellcome Fund (Career Award in the Biomedical Sciences), Rita Allen Foundation Scholar Award, Instituto Gulbenkian de Ciência, European Commission FP7 Program, EMBO

Gyakorlóiskola (1915) 7

Magyar Tanítóképző A Tanítóképző-intézeti Tanárok Országos Egyesületének közlönye - melléklet 30. évfolyam Budapest, 1915. szeptember h

Spectroscopy as a rapid detecting paprika powder adulteration

Paprika powder, a spice known for imparting flavour, colour and aroma in foods has recently become compromised by fraudulent activities involving diverse adulterants such as corn flour and has prompted quality assurance (QA) measures. Near infrared spectroscopy (NIRS) is a non-destructive method gaining grounds in QA applications. The study applied NIRS to detect paprika powder adulterated with corn flour. Chemometric evaluation spectra showed that NIRS could discriminate cornflour adulteration with 100% classification accuracy. Adulteration was also predicted with high accuracies coefficient of determination (R2CV) between 0.97 and 0.99 and low errors (0.72 g/100g), proving the future QA applications of NIRS

Conservation value of the native Hungarian wild grape (Vitis sylvestris Gmel.) evaluated by microsatellite markers

Wild grape (Vitis sylvestris GMEL.) has became a highly threatened species in Europe because of habitat loss, competition with alien grape species and intensive forest exploitation. Twenty-three Vitis spp. samples were analysed at 8 microsatellite loci to estimate the genetic diversity of the natural Vitis sylvestris specimens. In order to analyse the morphological traits and to perform molecular analysis, 11 native individuals were sampled from 6 remnant Hungarian habitats of the wild grape. To compare the genetic relationships among the wild specimens, samples from Turkish habitats, as well as cultivars of Vitis vinifera, Vitis labrusca and Vitis riparia were also included. Genetic diversity was higher within the Hungarian wild grape locations, with a mean of He = 0.74, which was higher that of samples originating from a larger area of Turkey, He = 0.69. Most of the Hungarian samples formed a well-defined, separate branch on the NJ tree. Based on the morphological traits and molecular analysis on the territory of Szentendre Island, formerly considered to be one of the largest native locations of wild grape, interspecific hybrids of Vitis sylvestris and Vitis riparia were identified. It can be concluded from the results that most of the Hungarian habitats studied could be valuable for the conservation. This paper reports on Hungarian Vitis sylvestris habitats, providing the first genetic study on diversity and on the relationship of Vitis sylvestris to other Vitis specimens, wild or cultivated, in the central part of the Carpathian Basin

1991 Archaeological Excavations at the Charles Carroll House in Annapolis, Maryland, 18AP45

This report provides a detailed summary of archaeological excavations that were conducted by Archaeology in Annapolis inside the ground story of the Charles Carroll House in Annapolis (18AP45) during the summer and fall of 1991. This project was initiated by Charles Carroll House of Annapolis, Inc. (CCHA), and was made possible through an agreement between CCHA and Historic Annapolis Foundation. It was designed as an initial phase of a larger project to restore the Carroll House to its late 18th-century appearance, while at the same time adding modern facilities to accomodate receptions, conferences, and other adaptive uses. These excavations were conducted between June and mid October of 1991, prior to interior house restoration, with monitoring of site restoration activities continuing well into 1992. Archaeologists, working with fieldschool students, and volunteers, tested all identified rooms in the house's ground story and then expanded excavations as deemed necessary and as time permitted. In designing the project and in preparing this final report, the staff followed the "Guidelines for Archaeological Investigations in Maryland" (McNarnara 1981). The report includes several levels of summaries (from descriptive summaries of soil levels excavated from the individual units (Appendix A), to interpretive room summaries) in an effort to make the data easily accessible and understandable to archaeologists and others interested in this site