Tessellations and Pattern Formation in Plant Growth and Development

The shoot apical meristem (SAM) is a dome-shaped collection of cells at the apex of growing plants from which all above-ground tissue ultimately derives. In Arabidopsis thaliana (thale cress), a small flowering weed of the Brassicaceae family (related to mustard and cabbage), the SAM typically contains some three to five hundred cells that range from five to ten microns in diameter. These cells are organized into several distinct zones that maintain their topological and functional relationships throughout the life of the plant. As the plant grows, organs (primordia) form on its surface flanks in a phyllotactic pattern that develop into new shoots, leaves, and flowers. Cross-sections through the meristem reveal a pattern of polygonal tessellation that is suggestive of Voronoi diagrams derived from the centroids of cellular nuclei. In this chapter we explore some of the properties of these patterns within the meristem and explore the applicability of simple, standard mathematical models of their geometry.Comment: Originally presented at: "The World is a Jigsaw: Tessellations in the Sciences," Lorentz Center, Leiden, The Netherlands, March 200

Organizational Factors that Contribute to Operational Failures in Hospitals

The performance gap between hospital spending and outcomes is indicative of inefficient care delivery. Operational failures—breakdowns in internal supply chains that prevent work from being completed—contribute to inefficiency by consuming 10% of nurses’ time (Hendrich et al. 2008, Tucker 2004). This paper seeks to identify organizational factors associated with operational failures, with a goal of providing insight into effective strategies for removal. We observed nurses on medical/ surgical units at two hospitals, shadowed support staff who provided materials, and interviewed employees about their internal supply chain’s performance. These activities created a database of 120 operational failures and the organizational factors that contributed to them. We found that employees believed their department’s performance was satisfactory, but poorly trained employees in other departments caused the failures. However, only 14% of the operational failures arose from errors or training. They stemmed instead from multiple organizationally-driven factors: insufficient workspace (29%), poor process design (23%), and a lack of integration in the internal supply chains (23%). Our findings thus suggest that employees are unlikely to discern the role that their department’s routines play in operational failures, which hinders solution efforts. Furthermore, in contrast to the “Pareto Principle” which advocates addressing “large” problems that contribute a disproportionate share of the cumulative negative impact of problems, the failures and causes were dispersed over a wide range of factors. Thus, removing failures will require deliberate cross-functional efforts to redesign workspaces and processes so they are better integrated with patients’ needs

Designed for Workarounds: A Qualitative Study of the Causes of Operational Failures in Hospitals

Frontline care providers in hospitals spend at least 10% of their time working around operational failures, which are situations where information, supplies, or equipment needed for patient care are insufficient. However, little is known about underlying causes of operational failures and what hospitals can do to reduce their occurrence. To address this gap, we examined the internal supply chains at two hospitals with the aim of discovering organizational factors that contribute to operational failures. We conducted in-depth qualitative research, including observations and interviews of over 80 individuals from 4 nursing units and the ancillary support departments that provide equipment and supplies needed for patient care. We found that a lack of interconnectedness among interdependent departments' routines was a major source of operational failures. The low levels of interconnectedness occurred because of how the internal supply chains were designed and managed rather than because of employee error or a shortfall in training. Thus, we propose that the time that hospital staff spend on workarounds can be reduced through deliberate efforts to increase interconnectedness among hospitals' internal supply departments. Four dimensions of interconnectedness include (1) hospital-level—rather than department-level—performance measures; (2) internal supply department routines that respond to specific patients' needs rather than to predetermined stocking routines; (3) knowledge that is necessary for efficient handoffs of materials is translated across departmental boundaries; and (4) cross-departmental collaboration mechanisms that enable improvement in the flow of materials across departmental boundaries

Star Formation in Sculptor Group Dwarf Irregular Galaxies and the Nature of "Transition" Galaxies

We present new H-alpha narrow band imaging of the HII regions in eight Sculptor Group dwarf irregular (dI) galaxies. Comparing the Sculptor Group dIs to the Local Group dIs, we find that the Sculptor Group dIs have, on average, lower values of SFR when normalized to either galaxy luminosity or gas mass (although there is considerable overlap between the two samples). The properties of ``transition'' (dSph/dIrr) galaxies in Sculptor and the Local Group are also compared and found to be similar. The transition galaxies are typically among the lowest luminosities of the gas rich dwarf galaxies. Relative to the dwarf irregular galaxies, the transition galaxies are found preferentially nearer to spiral galaxies, and are found nearer to the center of the mass distribution in the local cloud. While most of these systems are consistent with normal dI galaxies which currently exhibit temporarily interrupted star formation, the observed density-morphology relationship (which is weaker than that observed for the dwarf spheroidal galaxies) indicates that environmental processes such as ``tidal stirring'' may play a role in causing their lower SFRs.Comment: 35 pages, 10 figures, accepted for Feb 2003 AJ, companion to astro-ph/021117

Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

[EN] The genetic mechanisms underlying fruit development have been identified in Arabidopsis and have been comparatively studied in tomato as a representative of fleshy fruits. However, comparative expression and functional analyses on the bHLH genes downstream the genetic network, ALCATRAZ (ALC) and SPATULA (SPT), which are involved in the formation of the dehiscence zone in Arabidopsis, have not been functionally studied in the Solanaceae. Here, we perform detailed expression and functional studies of ALC/SPT homologs in Nicotiana obtusifolia with capsules, and in Capsicum annuum and Solanum lycopersicum with berries. In Solanaceae, ALC and SPT genes are expressed in leaves, and all floral organs, especially in petal margins, stamens and carpels; however, their expression changes during fruit maturation according to the fruit type. Functional analyses show that downregulation of ALC/SPT genes does not have an effect on gynoecium patterning; however, they have acquired opposite roles in petal expansion and have been co-opted in leaf pigmentation in Solanaceae. In addition, ALC/SPT genes repress lignification in time and space during fruit development in Solanaceae. Altogether, some roles of ALC and SPT genes are different between Brassicaceae and Solanaceae; while the paralogs have undergone some subfunctionalization in the former they are mostly redundant in the latter.This work was funded by COLCIENCIAS (111565842812), the iCOOP + 2016 COOPB20250 from the Centro Superior de Investigación Científica, CSIC, the ExpoSeed (H2020.MSCA-RISE-2015-691109) EU grant, the Convocatoria Programáticas 2017-16302, and the Estrategia de Sostenibilidad 2018-2019, from the Universidad de Antioquia. 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Control of surface plasmon resonance in out-diffused silver nanoislands for surface-enhanced Raman scattering

Abstract We present the studies of self-assembled silver nanoislands on the surface of silver ion-exchanged glasses. The nanoislands were formed by out-diffusion of reduced silver atoms from the bulk of the glass to its surface. Control of silver ions distribution in the glass by thermal poling after the ion exchange allowed formation of relatively big, up to 250 nm, isolated silver nanoislands while without the poling an ensemble of silver nanoislands with average size from several to tens of nanometers with random size distribution was formed. The nanoislands were characterized using atomic force microscopy and spectral measurements. We used optical absorption spectroscopy for “random” nanoislands and dark field scattering spectroscopy for isolated ones, corresponding spectra showed peaks in the vicinity of 450 nm and 600 nm, respectively. The “random” nanoislands significantly enhanced Raman scattering from Rhodamine 6G, also the modification of Raman signal from deposited on the surface of the samples bacteriorhodopsin in purple membranes was registered

Theoretical Modeling of Starburst Galaxies

We have modeled a large sample of infrared starburst galaxies using both the PEGASE v2.0 and STARBURST99 codes to generate the spectral energy distribution of the young star clusters. PEGASE utilizes the Padova group tracks while STARBURST99 uses the Geneva group tracks, allowing comparison between the two. We used our MAPPINGS III code to compute photoionization models which include a self-consistent treatment of dust physics and chemical depletion. We use the standard optical diagnostic diagrams as indicators of the hardness of the EUV radiation field in these galaxies. These diagnostic diagrams are most sensitive to the spectral index of the ionizing radiation field in the 1-4 Rydberg region. We find that warm infrared starburst galaxies contain a relatively hard EUV field in this region. The PEGASE ionizing stellar continuum is harder in the 1-4 Rydberg range than that of STARBURST99. As the spectrum in this regime is dominated by emission from Wolf-Rayet (W-R) stars, this difference is most likely due to the differences in stellar atmosphere models used for the W-R stars. We believe that the stellar atmospheres in STARBURST99 are more applicable to the starburst galaxies in our sample, however they do not produce the hard EUV field in the 1-4 Rydberg region required by our observations. The inclusion of continuum metal blanketing in the models may be one solution. Supernova remnant (SNR) shock modeling shows that the contribution by mechanical energy from SNRs to the photoionization models is << 20%. The models presented here are used to derive a new theoretical classification scheme for starbursts and AGN galaxies based on the optical diagnostic diagrams.Comment: 36 pages, 16 figures, to be published in ApJ, July 20, 200

Intracerebroventricular Catalase Reduces Hepatic Insulin Sensitivity and Increases Responses to Hypoglycemia in Rats

Specialized metabolic-sensors in the hypothalamus regulate blood glucose levels by influencing hepatic glucose output and hypoglycemic counter regulatory responses. Hypothalamic reactive oxygen species (ROS) may act as a metabolic signal mediating responses to changes in glucose, other substrates and hormones. The role of ROS in the brain's control of glucose homeostasis remains unclear. We hypothesized that hydrogen peroxide (H$_2$O$_2$), a relatively stable form of ROS, acts as a sensor of neuronal glucose consumption and availability and that lowering brain H$_2$O$_2$ with the enzyme catalase would lead to systemic responses increasing blood glucose. During hyperinsulinemic euglycemic clamps in rats, ICV catalase infusion resulted in increased hepatic glucose output, which was associated with reduced neuronal activity in the arcuate nucleus of the hypothalamus (ARC). Electrophysiological recordings revealed a subset of ARC neurons expressing pro-opiomelanocortin (POMC) that were inhibited by catalase and excited by H$_2$O$_2$. During hypoglycemic clamps, ICV catalase increased glucagon and epinephrine responses to hypoglycemia, consistent with perceived lower glucose levels. Our data suggest that H$_2$O$_2$ represents an important metabolic cue which, through tuning the electrical activity of key neuronal populations such as POMC neurons, may have a role in the brain's influence of glucose homeostasis and energy balance.This work was supported by the Juvenile Diabetes Research Foundation Grant 1-2006-29 and the Diabetes UK Grant RD05/ 003059 (to M.L.E.), the Wellcome Trust Grant WT098012 (to L.K.H.), and Cambridge Medical Research Council Centre for Study of Obesity and Related Disorders. In addition, PhD studentships/fellowships were supported for S.P.M. (Elmore Fund), P.H. (Sir Jules Thorn Trust), and C.-Y.Y. (Chang Gung University College of Medicine Grant numbers CMRPG6B0291 and CMRPG6B0292)