Evidence for regulated expression of Telomeric Repeat-containing RNAs (TERRA) in parasitic trypanosomatids

The Telomeric Repeat-containing RNAs (TERRA) participate in the homeostasis of telomeres in higher eukaryotes. Here, we investigated the expression of TERRA in Leishmania spp. and Trypanosoma brucei and found evidences for its expression as a specific RNA class. The trypanosomatid TERRA are heterogeneous in size and partially polyadenylated. The levels of TERRA transcripts appear to be modulated through the life cycle in both trypanosomatids investigated, suggesting that TERRA play a stage-specific role in the life cycle of these early-branching eukaryotes

Chemical abundances and properties of the ionized gas in NGC 1705

We obtained [O III] narrow-band imaging and multi-slit MXU spectroscopy of the blue compact dwarf (BCD) galaxy NGC 1705 with FORS2@VLT to derive chemical abundances of PNe and H II regions and, more in general, to characterize the properties of the ionized gas. The auroral [O III]\lambda4363 line was detected in all but one of the eleven analyzed regions, allowing for a direct estimate of their electron temperature. The only object for which the [O III]\lambda4363 line was not detected is a possible low-ionization PN, the only one detected in our data. For all the other regions, we derived the abundances of Nitrogen, Oxygen, Neon, Sulfur and Argon out to ~ 1 kpc from the galaxy center. We detect for the first time in NGC 1705 a negative radial gradient in the oxygen metallicity of -0.24 \pm 0.08 dex kpc^{-1}. The element abundances are all consistent with values reported in the literature for other samples of dwarf irregular and blue compact dwarf galaxies. However, the average (central) oxygen abundance, 12 + log(O/H)=7.96 \pm 0.04, is ~0.26 dex lower than previous literature estimates for NGC 1705 based on the [O III]\lambda4363 line. From classical emission-line diagnostic diagrams, we exclude a major contribution from shock excitation. On the other hand, the radial behavior of the emission line ratios is consistent with the progressive dilution of radiation with increasing distance from the center of NGC 1705. This suggests that the strongest starburst located within the central $\sim$150 pc is responsible for the ionization of the gas out to at least $\sim$1 kpc. The gradual dilution of the radiation with increasing distance from the center reflects the gradual and continuous transition from the highly ionized H II regions in the proximity of the major starburst into the diffuse ionized gas.Comment: Accepted for publication on A

Old open clusters: UBGVRI photometry of NGC 2506

UBGVRI photometry for the open cluster NGC 2506 is presented. From comparison of the observed colour-magnitude diagrams with simulations based on stellar evolutionary models we derive in a self consistent way reddening, distance, and age of the cluster: E(B-V)=0-0.07, (m-M)o = 12.6, age = 1.5-2.2 Gyr. The cluster shows a well definite secondary sequence, suggesting that binary systems constitute about 20 % of the cluster members visible in the colour-magnitude diagram.Comment: 11 pages, 7 figures, MNRAS latex style, accepte

Excised acoustic black holes: the scattering problem in the time domain

The scattering process of a dynamic perturbation impinging on a draining-tub model of an acoustic black hole is numerically solved in the time domain. Analogies with real black holes of General Relativity are explored by using recently developed mathematical tools involving finite elements methods, excision techniques, and constrained evolution schemes for strongly hyperbolic systems. In particular it is shown that superradiant scattering of a quasi-monochromatic wavepacket can produce strong amplification of the signal, offering the possibility of a significant extraction of rotational energy at suitable values of the angular frequency of the vortex and of the central frequency of the wavepacket. The results show that theoretical tools recently developed for gravitational waves can be brought to fruition in the study of other problems in which strong anisotropies are present.Comment: 8 pages, 9 figure

Fully Frustrated Cold Atoms

Fully frustrated Josephson Junction arrays (FF-JJA's) exhibit a subtle compound phase transition in which an Ising transition associated with discrete broken translational symmetry and a Berezinskii-Kosterlitz-Thouless (BKT) transition associated with quasi-long-range phase coherence occur nearly simultaneously. In this Letter we discuss a cold atom realization of the FF-JJA system. We demonstrate that both orders can be studied by standard momentum-distribution-function measurements and present numerical results, based on a successful self-consistent spin-wave approximation, that illustrate the expected behavior of observables.Comment: 5 pages, 3 figures, submitte

Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania

The Rad9-Rad1-Hus1 (9-1-1) complex is a key component in the coordination of DNA damage sensing, cell cycle progression and DNA repair pathways in eukaryotic cells. This PCNA-related trimer is loaded onto RPA-coated single stranded DNA and interacts with ATR kinase to mediate effective checkpoint signaling to halt the cell cycle and to promote DNA repair. Beyond these core activities, mounting evidence suggests that a broader range of functions can be provided by 9-1-1 structural diversification. The protozoan parasite Leishmania is an early-branching eukaryote with a remarkably plastic genome, which hints at peculiar genome maintenance mechanisms. Here, we investigated the existence of homologs of the 9-1-1 complex subunits in L. major and found that LmRad9 and LmRad1 associate with chromatin in response to replication stress and form a complex in vivo with LmHus1. Similar to LmHus1, LmRad9 participates in telomere homeostasis and in the response to both replication stress and double strand breaks. However, LmRad9 and LmHus1-deficient cells present markedly opposite phenotypes, which suggest their functional compartmentalization. We show that some of the cellular pool of LmRad9 forms an alternative complex and that some of LmHus1 exists as a monomer. We propose that the diverse assembly of the Leishmania 9-1-1 subunits mediates functional compartmentalization, which has a direct impact on the response to genotoxic stress

On the Ability of Functional Size Measurement Methods to Size Complex Software Applications

The most popular Functional Size Measurement methods, namely IFPUG Function Point Analysis and the COSMIC method, adopt a concept of \u201cfunctionality\u201d that is based mainly on the data involved in functions and data movements. Neither of the mentioned methods takes directly into consideration the amount of data processing involved in a process. Functional size measures are often used as a basis for estimating the effort required for software development, and it is known that development effort does depend on the amount of data processing code to be written. Thus, it is interesting to investigate to what extent the most popular functional size measures represent the functional processing features of requirements and, consequently, the amount of data processing code to be written. To this end, we consider a few applications that provide similar functionality, but require different amounts of data processing. These applications are then measured via both functional size measurement methods and traditional size measures (such as Lines of Code). A comparison of the obtained measures shows that differences among the applications are best represented by differences in Lines of Code. It is likely that the actual size of an application that requires substantial amounts of data processing is not fully represented by functional size measures. In summary, the paper shows that not taking into account data processing dramatically limits the expressiveness of the size measures. Practitioners that use size measures for effort estimation should complement functional size measures with measures that quantify data processing, to get precise effort estimates

The habitability of a stagnant-lid Earth

Plate tectonics is a fundamental component for the habitability of the Earth. Yet whether it is a recurrent feature of terrestrial bodies orbiting other stars or unique to the Earth is unknown. The stagnant lid may rather be the most common tectonic expression on such bodies. To understand whether a stagnant-lid planet can be habitable, i.e. host liquid water at its surface, we model the thermal evolution of the mantle, volcanic outgassing of H$_2$O and CO$_2$, and resulting climate of an Earth-like planet lacking plate tectonics. We used a 1D model of parameterized convection to simulate the evolution of melt generation and the build-up of an atmosphere of H$_2$O and CO$_2$ over 4.5 Gyr. We then employed a 1D radiative-convective atmosphere model to calculate the global mean atmospheric temperature and the boundaries of the habitable zone (HZ). The evolution of the interior is characterized by the initial production of a large amount of partial melt accompanied by a rapid outgassing of H$_2$O and CO$_2$. At 1 au, the obtained temperatures generally allow for liquid water on the surface nearly over the entire evolution. While the outer edge of the HZ is mostly influenced by the amount of outgassed CO$_2$, the inner edge presents a more complex behaviour that is dependent on the partial pressures of both gases. At 1 au, the stagnant-lid planet considered would be regarded as habitable. The width of the HZ at the end of the evolution, albeit influenced by the amount of outgassed CO$_2$, can vary in a non-monotonic way depending on the extent of the outgassed H$_2$O reservoir. Our results suggest that stagnant-lid planets can be habitable over geological timescales and that joint modelling of interior evolution, volcanic outgassing, and accompanying climate is necessary to robustly characterize planetary habitability