Development of large-internal-surface-area nickel-metal plaques third quarterly progress report, dec. 19, 1964 - mar. 18, 1965

Electrodes impregnated with cadmium for porosity and electrochemical evaluatio

Rapid Detection of Pathogenic Fungi from Clinical Specimens Using LightCycler Real-Time Fluorescence PCR

In the study presented here a LightCycler real-time PCR system was used for the diagnosis of fungal infections from clinical tissue samples. Nine specimens were investigated from six patients with suspected or proven invasive fungal infections. Seven of nine samples were positive in a broad-range fungal PCR assay. In four samples, Aspergillus fumigatus was detected both by a species-specific hybridization assay as well as by sequencing of amplification products. In addition, the broad-range fungal PCR assay and PCR sequencing detected and identified, respectively, the following organisms in the specimens noted: Candida albicans in a culture-negative liver biopsy, Histoplasma capsulatum in a bone marrow sample, and Conidiobolus coronatus in a facial soft tissue specimen. Real-time PCR is a promising tool for the diagnosis of invasive fungal infections in human tissue samples and offers some advantages over culture methods, such as rapid analysis and increased sensitivit

Ueber physikalische und chemische Veränderungen der Eisenoxydsalze in ihren Lösungen

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Query Expansion for Survey Question Retrieval in the Social Sciences

In recent years, the importance of research data and the need to archive and to share it in the scientific community have increased enormously. This introduces a whole new set of challenges for digital libraries. In the social sciences typical research data sets consist of surveys and questionnaires. In this paper we focus on the use case of social science survey question reuse and on mechanisms to support users in the query formulation for data sets. We describe and evaluate thesaurus- and co-occurrence-based approaches for query expansion to improve retrieval quality in digital libraries and research data archives. The challenge here is to translate the information need and the underlying sociological phenomena into proper queries. As we can show retrieval quality can be improved by adding related terms to the queries. In a direct comparison automatically expanded queries using extracted co-occurring terms can provide better results than queries manually reformulated by a domain expert and better results than a keyword-based BM25 baseline.Comment: to appear in Proceedings of 19th International Conference on Theory and Practice of Digital Libraries 2015 (TPDL 2015

The CODE ambiguity-fixed clock and phase bias analysis products: generation, properties, and performance

The generation and use of GNSS analysis products that allowparticularly for the needs of single-receiver applicationsprecise point positioning with ambiguity resolution (PPP-AR) are becoming more and more popular. A general uncertainty concerns the question on how the necessary phase bias information should be provided to the PPP-AR user. Until now, each AR-enabling clock/bias representation method had its own practice to provide the necessary bias information. We have generalized the observable-specific signal bias (OSB) representation, as introduced in Villiger (J Geod 93:14871500, 2019) originally exclusively for pseudorange measurements, to carrier phase measurements. The existing common clock (CC) approach has been extended in a way that OSBs allowing for flexible signal and frequency handling between multiple GNSS become possible. Advantages of the proposed OSB-based PPP-AR approach are: GNSS biases can be provided in a consistent way for phase and code measurements and it is capable of multi-GNSS and suitable for standardization. This new, extended PPP-AR approach has been implemented by the Center for Orbit Determination in Europe (CODE). CODE clock products that adhere to the integer-cycle property have been submitted to the International GNSS Service (IGS) since mid of 2018 for three analysis lines: Rapid, Final, and MGEX (Multi-GNSS Extension). Ambiguity fixing is performed not only for GPS but also for Galileo. The integer-cycle property of between-satellite clock differences is of fundamental importance when comparing satellite clock estimates among various analysis lines, or at day boundaries. Both kinds of comparisons could be exploited at a very high level of consistency. Any retrieved comparison essentially indicated a standard deviation for between-satellite clocks from CODE of the order of 5 ps (1.5 mm in range). Finally, the integer-cycle property that may be recovered between the CODE Final clock and the accompanying bias product of consecutive daily sessions (using clock estimates additionally provided for the second midnight epoch) allows us to deduce GPS satellite clock and phase bias information that is consistent and continuous with respect to carrier phase observation data over two, three, or, in principle, yet more days. Phase-based clock densification from initially estimated integer-cycle-conform clock corrections at intervals of 300 s to 30 s (5 s in case of our Final clock product) is a matter of particular interest. Based on direct product comparisons and GRACE K-band ranging (KBR) data analysis, the quality of accordingly densified clock corrections could be confirmed to be on a level similar to that of of “anchor” (300s) clock corrections

Role of homologous ASP334 and GLU319 in human non-gastric H,K- and Na,K-ATPases in cardiac glycoside binding

Cardiac steroids inhibit Na,K-ATPase and the related non-gastric H,K-ATPase, while they do not interact with gastric H,K-ATPase. Introducing an arginine, the residue present in the gastric H,K-ATPase, in the second extracellular loop at the corresponding position 334 in the human non-gastric H,K-ATPase (D334R mutation) rendered it completely resistant to 2mM ouabain. The corresponding mutation (E319R) in alpha1 Na,K-ATPase produced a approximately 2-fold increase of the ouabain IC(50) in the ouabain-resistant rat alpha1 Na,K-ATPase and a large decrease of the ouabain affinity of human alpha1 Na,K-ATPase, on the other hand this mutation had no effect on the affinity for the aglycone ouabagenin. These results provide a strong support for the orientation of ouabain in its biding site with its sugar moiety interacting directly with the second extracellular loop

Development of large-internal-surface-area nickel-metal plaques Final report, Jun. 18, 1964 - Sep. 30, 1965

Large internal surface area porous nickel metal plaques for rechargeable cadmium electrodes to improve nickel-cadmium batterie

Rapid Detection of Pathogenic Fungi from Clinical Specimens Using LightCycler Real-Time Fluorescence PCR

In the study presented here a LightCycler real-time PCR system was used for the diagnosis of fungal infections from clinical tissue samples. Nine specimens were investigated from six patients with suspected or proven invasive fungal infections. Seven of nine samples were positive in a broad-range fungal PCR assay. In four samples, Aspergillus fumigatus was detected both by a species-specific hybridization assay as well as by sequencing of amplification products. In addition, the broad-range fungal PCR assay and PCR sequencing detected and identified, respectively, the following organisms in the specimens noted: Candida albicans in a culture-negative liver biopsy, Histoplasma capsulatum in a bone marrow sample, and Conidiobolus coronatus in a facial soft tissue specimen. Real-time PCR is a promising tool for the diagnosis of invasive fungal infections in human tissue samples and offers some advantages over culture methods, such as rapid analysis and increased sensitivit

Hemophagocytic syndrome caused by primary herpes simplex virus 1 infection: report of a first case

Introduction: Hemophagocytic syndrome represents a severe hyperinflammatory condition by activated macrophages. Leading viral triggering agents are Epstein-Barr virus (EBV), cytomegalovirus (CMV), and adenovirus. Materials and methods: We present a patient with Wegener's granulomatosis on azathioprine and prednisone medication, who developed a life-threatening hemophagocytic syndrome. Positive plasma polymerase chain reaction (PCR) with negative serology revealed a primary, disseminated infection with herpes simplex virus-1 as the triggering pathogen. After treatment with acyclovir, high-dose steroids, immunoglobulins, and etoposide, the patient recovered. Conclusion: Early diagnosis of potentially underlying infections of hemophagocytic syndrome influences the therapeutic approach. It is important to consider a variety of infectious agents, particularly in immunosuppressed individuals. The reported case emphasizes the importance of screening for herpes simplex virus