Comparison and relative utility of inequality measurements: as applied to Scotland’s child dental health

This study compared and assessed the utility of tests of inequality on a series of very large population caries datasets. National cross-sectional caries datasets for Scotland’s 5-year-olds in 1993/94 (n = 5,078); 1995/96 (n = 6,240); 1997/98 (n = 6,584); 1999/00 (n = 6,781); 2002/03 (n = 9,747); 2003/04 (n = 10,956); 2005/06 (n = 10,945) and 2007/08 (n = 12,067) were obtained. Outcomes were based on the d3mft metric (i.e. the number of decayed, missing and filled teeth). An area-based deprivation category (DepCat) measured the subjects’ socioeconomic status (SES). Simple absolute and relative inequality, Odds Ratios and the Significant Caries Index (SIC) as advocated by the World Health Organization were calculated. The measures of complex inequality applied to data were: the Slope Index of Inequality (absolute) and a variety of relative inequality tests i.e. Gini coefficient; Relative Index of Inequality; concentration curve; Koolman and Doorslaer’s transformed Concentration Index; Receiver Operator Curve and Population Attributable Risk (PAR). Additional tests used were plots of SIC deciles (SIC10) and a Scottish Caries Inequality Metric (SCIM10). Over the period, mean d3mft improved from 3.1(95%CI 3.0–3.2) to 1.9(95%CI 1.8–1.9) and d3mft = 0% from 41.1(95%CI 39.8–42.3) to 58.3(95%CI 57.8–59.7). Absolute simple and complex inequality decreased. Relative simple and complex inequality remained comparatively stable. Our results support the use of the SII and RII to measure complex absolute and relative SES inequalities alongside additional tests of complex relative inequality such as PAR and Koolman and Doorslaer’s transformed CI. The latter two have clear interpretations which may influence policy makers. Specialised dental metrics (i.e. SIC, SIC10 and SCIM10) permit the exploration of other important inequalities not determined by SES, and could be applied to many other types of disease where ranking of morbidity is possible e.g. obesity. More generally, the approaches described may be applied to study patterns of health inequality affecting worldwide populations

Continuous intestinal infusion of levodopa-carbidopa in patients with advanced Parkinson's disease in Spain: Subanalysis by autonomous community

Objetivos: Comparar las características de los pacientes con enfermedad de Parkinson avan- zada en tratamiento con infusión intestinal continua de levodopa-carbidopa (IICLC) y los datos de efectividad y seguridad de IICLC entre diferentes comunidades autónomas (CC. AA.). Métodos: Estudio longitudinal observacional y retrospectivo. Se incluyeron 177 pacientes de 11 CC. AA. que iniciaron tratamiento con IICLC entre enero de 2006 y diciembre de 2011. Se compararon las características clínicas y demográficas, las variables de efectividad (cambios en el tiempo OFF, ON con y sin discinesias discapacitantes, cambios en la escala de Hoehn y Yahr y puntuación de la Unified Parkinson’s Disease Rating Scale, síntomas no motores e Impresión Clínica Global) y seguridad (acontecimientos adversos), y la tasa de suspensión de IICLC. Resultados: Se hallaron diferencias significativas entre las CC. AA. en diversas variables basa- les: duración de la enfermedad hasta el inicio de IICLC, tiempo OFF (34,9-59,7%) y ON (con o sin discinesias; 2,6-48,0%), Hoehn y Yahr en ON, Unified Parkinson’s Disease Rating Scale-III en ON y OFF, presencia de ≥ 4 síntomas motores y dosis de IICLC. En el seguimiento (> 24 meses en 9 de 11 CC. AA.) hubo diferencias significativas en el porcentaje de tiempo OFF, tiempo ON sin discinesias discapacitantes, frecuencia de acontecimientos adversos e Impresión Clínica Global. La tasa de suspensión fue de entre 20-40% en todas las CC. AA., excepto en 2 (78 y 80%). Conclusiones: Este estudio muestra una amplia variabilidad en la selección de los pacientes y en la efectividad y seguridad de IICLC entre las diferentes CC. AA. Podrían influir las caracterís- ticas basales de los pacientes, la disponibilidad de un equipo multidisciplinar y la experiencia clínica.Objectives: To compare the characteristics of patients undergoing treatment with continuous intestinal infusion of levodopa-carbidopa (CIILC) for advanced Parkinson’s disease and the data on the effectiveness and safety of CIILC in the different autonomous communities (AC) of Spain. Methods: A retrospective, longitudinal, observational study was carried out into 177 patients from 11 CAs who underwent CIILC between January 2006 and December 2011. We analysed data on patients’ clinical and demographic characteristics, variables related to effectiveness (chan- ges in off time/on time with or without disabling dyskinesia; changes in Hoehn and Yahr scale and Unified Parkinson’s Disease Rating Scale scores; non-motor symptoms; and Clinical Global Impression scale scores) and safety (adverse events), and the rate of CIILC discontinuation. Results: Significant differences were observed between CAs for several baseline variables: duration of disease progression prior to CIILC onset, off time (34.9-59.7%) and on time (2.6- 48.0%; with or without disabling dyskinesia), Hoehn and Yahr score during on time, Unified Parkinson’s Disease Rating Scale-III score during both on and off time, presence of ≥ 4 motor symptoms, and CIILC dose. Significant differences were observed during follow-up (> 24 months in 9 of the 11 CAs studied) for the percentage of off time and on time without disabling dys- kinesia, adverse events frequency, and Clinical Global Impression scores. The rate of CIILC discontinuation was between 20-40% in 9 CAs (78 and 80% in remaining 2 CAs). Conclusions: This study reveals a marked variability between CAs in terms of patient selection and CIILC safety and effectiveness. These results may have been influenced by patients’ baseline characteristics, the availability of multidisciplinary teams, and clinical experience

Integrative transcriptome and proteome analyses define marked differences between Neospora caninum isolates throughout the tachyzoite lytic cycle

Neospora caninum is one of the main causes of transmissible abortion in cattle. Intraspecific variations in virulence have been widely shown among N. caninum isolates. However, the molecular basis governing such variability have not been elucidated to date. In this study label free LC-MS/MS was used to investigate proteome differences between the high virulence isolate Nc-Spain7 and the low virulence isolate Nc-Spain1H throughout the tachyzoite lytic cycle. The results showed greater differences in the abundance of proteins at invasion and egress with 77 and 62 proteins, respectively. During parasite replication, only 19 proteins were differentially abundant between isolates. The microneme protein repertoire involved in parasite invasion and egress was more abundant in the Nc-Spain1H isolate, which displays a lower invasion rate. Rhoptry and dense granule proteins, proteins related to metabolism and stress responses also showed differential abundances between isolates. Comparative RNA-Seq analyses during tachyzoite egress were also performed, revealing an expression profile of genes associated with the bradyzoite stage in the low virulence Nc-Spain1H isolate. The differences in proteome and RNA expression profiles between these two isolates reveal interesting insights into likely mechanisms involved in specific phenotypic traits and virulence in N. caninum. Significance The molecular basis that governs biological variability in N. caninum and the pathogenesis of neosporosis has not been well-established yet. This is the first study in which high throughput technology of LC-MS/MS and RNA-Seq is used to investigate differences in the proteome and transcriptome between two well-characterized isolates. Both isolates displayed different proteomes throughout the lytic cycle and the transcriptomes also showed marked variations but were inconsistent with the proteome results. However, both datasets identified a pre-bradyzoite status of the low virulence isolate Nc-Spain1H. This study reveals interesting insights into likely mechanisms involved in virulence in N. caninum and shed light on a subset of proteins that are potentially involved in the pathogenesis of this parasite

Assessing non–parametric estimators of species richness. A case study with birds in green areas of the city of Puebla, Mexico

Nuestro objetivo fue evaluar el desempeño de estimadores no paramétricos de la riqueza de especies con datos reales. Durante la temporada de cría de 2003 censamos las comunidades de aves en dos áreas verdes de la ciudad de Puebla (México), y obtuvimos las corres¬pondientes curvas de rarefacción, que fueron ajustadas a dos funciones de acumulación de especies no asintóticas y siete asintóticas. Según criterios de la teoría de la información, la función de acumulación de Weibull o la Beta–P fueron las que mejor describieron estas curvas de acumulación, y asumimos que sus asíntotas estimaron la riqueza real en los dos sitios. Después evaluamos el sesgo, la precisión y la exactitud de cinco estimadores no paramétricos de la riqueza de especies (ICE, Chao 2, Jackknife 1, Jackknife 2 y Bootstrap) para esfuerzos de muestreo crecientes (1–53 unidades de censo). Todos los estimadores no paramétricos aquí evaluados subestimaron la riqueza asintótica la mayor parte del tiempo, en especial en una de las comunidades. Sin embargo, tras combinar los datos de los dos sitios, sólo ICE, Jackknife 1 y Jackknife 2 mostraron sesgos menores al 10% con algún esfuerzo de muestreo, aunque únicamente Jackknife 1 tuvo una exactitud global alta (error medio relativo al cuadradox100 &lt; 5%), incluso con esfuerzos de muestreo bajos (cerca del 20% del total de las unidades de censo). En conclusión, proponemos que el estimador no paramétrico Jackknife 1 puede usarse como un límite inferior de la riqueza de especies de aves en áreas urbanas similares a las de nuestro estudio. Palabras clave: Exactitud, Sesgo, Biodiversidad, Aves, Inventarios, Jackknife, Precisión, Urbanización.Nuestro objetivo fue evaluar el desempeño de estimadores no paramétricos de la riqueza de especies con datos reales. Durante la temporada de cría de 2003 censamos las comunidades de aves en dos áreas verdes de la ciudad de Puebla (México), y obtuvimos las corres¬pondientes curvas de rarefacción, que fueron ajustadas a dos funciones de acumulación de especies no asintóticas y siete asintóticas. Según criterios de la teoría de la información, la función de acumulación de Weibull o la Beta–P fueron las que mejor describieron estas curvas de acumulación, y asumimos que sus asíntotas estimaron la riqueza real en los dos sitios. Después evaluamos el sesgo, la precisión y la exactitud de cinco estimadores no paramétricos de la riqueza de especies (ICE, Chao 2, Jackknife 1, Jackknife 2 y Bootstrap) para esfuerzos de muestreo crecientes (1–53 unidades de censo). Todos los estimadores no paramétricos aquí evaluados subestimaron la riqueza asintótica la mayor parte del tiempo, en especial en una de las comunidades. Sin embargo, tras combinar los datos de los dos sitios, sólo ICE, Jackknife 1 y Jackknife 2 mostraron sesgos menores al 10% con algún esfuerzo de muestreo, aunque únicamente Jackknife 1 tuvo una exactitud global alta (error medio relativo al cuadradox100 &lt; 5%), incluso con esfuerzos de muestreo bajos (cerca del 20% del total de las unidades de censo). En conclusión, proponemos que el estimador no paramétrico Jackknife 1 puede usarse como un límite inferior de la riqueza de especies de aves en áreas urbanas similares a las de nuestro estudio. Palabras clave: Exactitud, Sesgo, Biodiversidad, Aves, Inventarios, Jackknife, Precisión, Urbanización.Our objective was to evaluate the performance of non–parametric estimators of spe¬cies richness with real data. During the 2003 breeding season, bird communities were sampled in two green areas in the city of Puebla (Mexico), and the corresponding sample–based rarefaction curves were obtained. Mean data were adjusted to two non–asymptotic and seven asymptotic accumulation functions, and the best model was selected by means of reliability criteria in information theory. The cumulative Weibull and the Beta–P functions were the best–fit models. Bias, precision and accuracy of five non–parametric estimators of species richness (ICE, Chao2, Jackknife 1, Jackknife 2, and Bootstrap) were then assessed for increasing sampling efforts (1–53 sampling units) against the asymptote of the selected accumulation functions. All the non–parametric estimators here evaluated underestimated true richness most of the time, specially in one of the sites. However, after combining data from the two assemblages, only ICE, and Jackknife 1 and 2 exhibited bias below 10% with different sampling efforts, and only Jackknife 1 was globally accurate (scaled mean squared errorx100< 5%, even with low sampling efforts, ca. 20% of the total). Therefore, we propose using the Jackknife 1 non–parametric estimator as a lower limit to measure bird species richness in urban sites similar to those in the present study. Key words: Accuracy, Bias, Biodiversity, Birds, Inventories, Jackknife, Precision, Urbanization

Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by

Despite the importance of bovine neosporosis, relevant knowledge gaps remain concerning the pathogenic mechanisms of . Infection of the placenta is a crucial event in the pathogenesis of the disease; however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3 in terms of proliferative capacity and transcriptome host cell modulation. Herein, we used the same model of infection to study the interaction of with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analysed the proteome differences between high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates. The results showed that mitochondrial processes and metabolism were the main points of -host interactions. Interestingly, Nc-Spain1H infection showed a higher level of influence on the host cell proteome than Nc-Spain7 infection

Cancer mortality by educational level in the city of Barcelona

The objective of this study was to examine the relationship between educational level and mortality from cancer in the city of Barcelona. The data were derived from a record linkage between the Barcelona Mortality Registry and the Municipal Census. The relative risks (RR) of death and 95% confidence intervals (CIs) according to level of education were derived from Poisson regression models. For all malignancies, men in the lowest educational level had a RR of death of 1.21 (95% CI 1.13–1.29) compared with men with a university degree, whereas for women a significant decreasing in risk was observed (RR 0.81; 95% CI 0.74–0.90). Among men, significant negative trends of increasing risk according to level of education were present for cancer of the mouth and pharynx (RR 1.70 for lowest vs. highest level of education), oesophagus (RR 2.14), stomach (RR 1.99), larynx (RR 2.56) and lung (RR 1.35). Among women, cervical cancer was negatively related to education (RR 2.62), whereas a positive trend was present for cancers of the colon (RR 0.76), pancreas (RR 0.59), lung (RR 0.55) and breast (RR 0.65). The present study confirms for the first time, at an individual level, the existence of socioeconomic differences in mortality for several cancer sites in Barcelona, Spain. There is a need to implement health programmes and public health policies to reduce these inequities. © 1999 Cancer Research Campaig

Infected Dendritic Cells Facilitate Systemic Dissemination and Transplacental Passage of the Obligate Intracellular Parasite Neospora caninum in Mice

The obligate intracellular parasite Neospora caninum disseminates across the placenta and the blood-brain barrier, to reach sites where it causes severe pathology or establishes chronic persistent infections. The mechanisms used by N. caninum to breach restrictive biological barriers remain elusive. To examine the cellular basis of these processes, migration of different N. caninum isolates (Nc-1, Nc-Liverpool, Nc-SweB1 and the Spanish isolates: Nc-Spain 3H, Nc-Spain 4H, Nc-Spain 6, Nc-Spain 7 and Nc-Spain 9) was studied in an in vitro model based on a placental trophoblast-derived BeWo cell line. Here, we describe that infection of dendritic cells (DC) by N. caninum tachyzoites potentiated translocation of parasites across polarized cellular monolayers. In addition, powered by the parasite's own gliding motility, extracellular N. caninum tachyzoites were able to transmigrate across cellular monolayers. Altogether, the presented data provides evidence of two putative complementary pathways utilized by N. caninum, in an isolate-specific fashion, for passage of restrictive cellular barriers. Interestingly, adoptive transfer of tachyzoite-infected DC in mice resulted in increased parasitic loads in various organs, e.g. the central nervous system, compared to infections with free parasites. Inoculation of pregnant mice with infected DC resulted in an accentuated vertical transmission to the offspring with increased parasitic loads and neonatal mortality. These findings reveal that N. caninum exploits the natural cell trafficking pathways in the host to cross cellular barriers and disseminate to deep tissues. The findings are indicative of conserved dissemination strategies among coccidian apicomplexan parasites

Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks

© The Author(s) 2020.[Background]: Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain.[Methods]: Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes.[Results]: As a result, 30 isolates were obtained from 9 Spanish regions: 10 isolates from abortion-derived samples and 20 isolates from adult myocardial tissues. Overall, 3 genotypes were found: ToxoDB#3 (type II PRU variant) in 90% (27/30) of isolates, ToxoDB#2 (clonal type III) in 6.7% (2/30), and ToxoDB#1 (clonal type II) in 3.3% (1/30). When T. gondii-positive tissue samples (n = 151) were directly subjected to RFLP genotyping, complete restriction profiles were obtained for 33% of samples, and up to 98% of the specimens belonged to the type II PRU variant. A foetal brain showed a clonal type II pattern, and four specimens showed unexpected type I alleles at the SAG3 marker, including two foetal brains that showed I + II alleles as co-infection events. Amplicons of SAG3, GRA6 and GRA7 obtained from isolates and clinical samples were subjected to sequencing, allowing us to confirm RFLP results and to detect different single-nucleotide polymorphisms.[Conclusions]: The present study informed the existence of a predominant type II PRU variant genotype (ToxoDB#3) infecting domestic sheep in Spain, in both abortion cases and chronic infections in adults, coexisting with other clonal (ToxoDB#1 and ToxoDB#2), much less frequent genotypes, as well as polymorphic strains as revealed by clinical sample genotyping. The use of multilocus sequence typing aided in accurately estimating T. gondii intragenotype diversity.This research was supported by projects funded by the Spanish Ministry of Science and Innovation (AGL2016-75935-C2-R) and the Community of Madrid (PLATESA2-CM-P2018/BAA-4370). MF and RC were funded by UCM-Santander/2017 pre-doctoral grants, and PLATESA2 post-doctoral grants, respectively. CG was funded by DGAPA, National Autonomous University of Mexico (UNAM). RC, EC and LO are part of the TOXOSOURCES consortium, supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No. 773830: One Health European Joint Programme.Peer reviewe

Impact of increased resolution on long-standing biases in HighResMIP-PRIMAVERA climate models

We examine the influence of increased resolution on four long-standing biases using five different climate models developed within the PRIMAVERA project. The biases are the warm eastern tropical oceans, the double Intertropical Convergence Zone (ITCZ), the warm Southern Ocean, and the cold North Atlantic. Atmosphere resolution increases from ∼100–200 to ∼25–50 km, and ocean resolution increases from (eddy-parametrized) to (eddy-present). For one model, ocean resolution also reaches ∘ (eddy-rich). The ensemble mean and individual fully coupled general circulation models and their atmosphere-only versions are compared with satellite observations and the ERA5 reanalysis over the period 1980–2014. The four studied biases appear in all the low-resolution coupled models to some extent, although the Southern Ocean warm bias is the least persistent across individual models. In the ensemble mean, increased resolution reduces the surface warm bias and the associated cloud cover and precipitation biases over the eastern tropical oceans, particularly over the tropical South Atlantic. Linked to this and to the improvement in the precipitation distribution over the western tropical Pacific, the double-ITCZ bias is also reduced with increased resolution. The Southern Ocean warm bias increases or remains unchanged at higher resolution, with small reductions in the regional cloud cover and net cloud radiative effect biases. The North Atlantic cold bias is also reduced at higher resolution, albeit at the expense of a new warm bias that emerges in the Labrador Sea related to excessive ocean deep mixing in the region, especially in the ORCA025 ocean model. Overall, the impact of increased resolution on the surface temperature biases is model-dependent in the coupled models. In the atmosphere-only models, increased resolution leads to very modest or no reduction in the studied biases. Thus, both the coupled and atmosphere-only models still show large biases in tropical precipitation and cloud cover, and in midlatitude zonal winds at higher resolutions, with little change in their global biases for temperature, precipitation, cloud cover, and net cloud radiative effect. Our analysis finds no clear reductions in the studied biases due to the increase in atmosphere resolution up to 25–50 km, in ocean resolution up to 0.25∘, or in both. Our study thus adds to evidence that further improved model physics, tuning, and even finer resolutions might be necessary