137 research outputs found
Diagnostic significance of ultrasonographic score in detection of gestational diabetes mellitus
Cilj: Evaluirati dijagnostiÄku pouzdanost i efikasnost ultrazvuÄnih markera gestacionog
dijabetesa (GDM) i predloženog ultrazvuÄnog skora u otkrivanju GDM-a. Metode: 132
trudnice sa jednoplodnim trudnoÄama gestacione starosti veÄe od 24. nedelje gestacije i
prisutnim najmanje jednim faktorom rizika za GDM, bilo maternalnim ili vezanog za
trudnoÄu, podvrgnute su ultrazvuÄnom pregledu, a nakon toga oralnom testu
optereÄenja sa 100 g glukoze (oGTT). UltrazvuÄni marker mereni ili determinisani
tokom pregleda su: fetalno potkožno masno tkivo, asimetriÄna makrozomija, obim i
Ŕirina fetalnog srca, debljina interventrikularnog septuma, nezreli placentalni izgled,
intenzivirani fetalni disajni pokreti, polihidramnion i placentalna debljina. Svaki od
prisutnih pomenutih markera dobio je jedan poen, a u cilju formiranja ultrazvuÄnog
skora za otkrivanje Å”eÄerne bolesti trudnica (UGDS). Rezultati: Svi ultrazvuÄni markeri
GDM-a su pozitivno korelirani sa boleÅ”Äu, P < 0.0001. NajjaÄi nezavisni prediktor GDMa
je nezreli placentalni izgled (RR 49.09 95% CI 7,04-342,28, P < 0.0001). Receiver
operator characteristics (ROC) analiza demonstrirala je povrŔinu ispod krive od 96.9%,
potvrÄujuÄi dobru sposobnost UGDS-a da razlikuje patoloÅ”ke od normalnih vrednosti
oGTT-a. Kao graniÄna vrednost UGDS-a predložen je skor jednak ili veÄi od 4, koji
rezultuje sveukupnom taÄnoÅ”Äu od 92%, senzitivnoÅ”Äu od 93.2%, specifiÄnoÅ”Äu od 92%,
pozitivnom prediktivnom vrednoÅ”Äu od 85,4% i negativnom prediktivnom vrednoÅ”Äu
od 96,4%. ZakljuÄci: Rezultati disertacije sugeriÅ”u da je UGDS dobar prediktor GDM-a.
UGDS može biti dodatak trenutnim skrining metodama za detekciju GDM-a...Objective: To evaluate diagnostic accuracy and efficiency of ultrasound markers of
gestational diabetes (GDM) and propose an ultrasound based scoring system suitable
for screening (UGDS). Methods: 132 women with singleton pregnancies and established
maternal and/or pregnancy related risk factors for GDM were scanned at/or after 24
weeks gestation followed by administration of a 3 hour 100-gram oral glucose tolerance
test (oGTT). A number of ultrasound markers were determined/measured, including
fetal adipose subcutaneous tissue, asymmetrical macrosomy, cardiac circumference,
cardiac width, and interventricular septum thickness, immature appearance of placenta,
intensified breathing movements, polyhydramnios and placental thickness. Each
ultrasound GDM marker was assigned one point to create the ultrasound gestational
diabetes screening score (UGDS). Results: All ultrasound GDM markers were positively
correlated to the disease P < 0.0001. The strongest independent predictor of GDM was
an immature appearance of placenta (RR 49.09 95% CI 7,04-342,28, P < 0.0001). Receiver
operator characteristics (ROC) showed an area under the curve of 96.9% confirming
good ability of UGDS to discriminate between positive and negative oGTT. We propose
a UGDS score of 4 providing diagnostic efficiency of 92%, sensitivity 93.2%, specificity
92%, positive predictive value 85,4% and negative predictive value 96,4%. Conclusions:
This study suggests that UGDS is a good predictor of GDM. UGDS may be an adjunct to
current screening tools for GDM..
6-hydroxydopamine lesions of the striatum lead to the alterations of dopamine receptor mrna in parkinsonian rats
The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.Ispitivani su efekti Äetiri ubodne 6-hidroksidopaminske (6-OHDA) lezije striatuma kod odraslih mužjaka pacova. Pet dana nakon lezije, životinje su testirane na specifiÄno rotaciono ponaÅ” anje pod uticajem srednje doze amfetamina i rezultati su potvrdili efikasnost lezije. RNK iz striatuma su izolovane u razliÄitim vremenskim taÄkama nakon lezije i uraÄena je RT-PCR analiza iRNK za D1 i D2 dopaminske receptore. Rezultati pokazuju smanjivanje nivoa iRNK za D2 receptor (40%) 6 h i 24 h nakon lezije, dok sedam dana nakon lezije nema promena. Za razliku od ovih rezultata, u nivou iRNK za D1 receptor ne postoje statistiÄki znaÄajne razlike u bilo kojoj vremenskoj taÄki
Employing machine learning to assess the accuracy of near-infrared spectroscopy of spent dialysate fluid in monitoring the blood concentrations of uremic toxins
Hemodialysis (HD) removes nitrogenous waste products from patientsā blood through a semipermeable mem- brane along a concentration gradient. Near-infrared spectroscopy (NIRS) is an underexplored method of monitoring the concentrations of several molecules that reflect the efficacy of the HD process in dialysate samples. In this study, we aimed to evaluate NIRS as a technique for the non-invasive detection of uremic solutes by assessing the correlations between the spectrum of the spent dialysate and the serum levels of urea, creatinine, and uric acid. Blood and dialysate samples were taken from 35 patients on maintenance HD. The absorption spectrum of each dialysate sample was measured three times in the wavelength range of 700-1700 nm, resulting in a dataset with 315 spectra. The artificial neural network (ANN) learn- ing technique was used to assess the correlations between the recorded NIR-absorbance spectra of the spent dialysate and serum levels of selected uremic toxins. Very good correlations between the NIR-absorbance spectra of the spent dialysate fluid with serum urea (R=0.91) and uric acid (R=0.91) and an excellent correlation with serum creatinine (R=0.97) were obtained. These results support the application of NIRS as a non-invasive, safe, accurate, and repetitive technique for online monitoring of uremic toxins to assist clinicians in assessing HD efficiency and individualization of HD treatments
Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials - MagBioVin Project
Research advancements and opportunities by the FP7-ERA Chairs project MagBioVin are spotlighted.[1] Topic of the project is the design of different novel magnetic nanoarchitectures (e.g. bimagnetic and polymeric core-shell systems, nanoparticles embedded in mesoporous silica structures, and radiolabeled nanostructures)[2ā4] for application in targeted treatment and diagnostics of cancer. These nanomaterials posses the ability for selective treatment of tumor tissues by the targeting with magnetic field.[5,6] Alternating magnetic field also provides the means for hyperthermia-induced cancer treatment.[7]Attachment of radionuclides to the synthesized nanoparticles is explored for the purpose of imaging and internal radiotherapy.[8,9] Magnetic characteristics of the prepared nanomaterials is done by SQUID magnetometry and Mƶssbauer spectroscopy. Structural characterization of the investigated nanomaterials is performed by XRD, TEM imaging, DRIFT spectroscopy, and nitrogen sorption analysis. Magnetic hyperthermia effects are monitored by using commercial setup (nB nanoScale Biomagnetics) which includes applicators for cell cultures and small animals. In vitro and in vivo (animal model) applicability of the synthesized nanomaterials regarding toxicity, biodistribution and anticancer efficacy is explored for targeted cancer treatment.1st International Symposium: program and the book of abstracts : March 29-31, 2016; University of Central Lancashire, Preston, U
Changes in expression of GFAP, ApoE and APP mRNA and protein levels in the adult rat brain following cortical injury
The recovery period following cortical injury (CI) is characterized by a dynamic and highly complex interplay between beneficial and detrimental events. The aim of this study was to examine the expressions of Glial Fibrillary Acidic Protein (GFAP), Apolipoprotein E (ApoE) and Amyloid Precursor Protein (APP), all of which are involved in brain plasticity and neurodegeneration. Our results reveal that CI strongly influenced GFAP, ApoE and APP mRNA expression, as well as GFAP and ApoE protein expression. Considering the pivotal role of these proteins in the brain, the obtained results point to their potential contribution in neurodegeneration and consequent Alzheimer's disease development.Projekat ministarstva br. 17305
Fatty acid in colorectal cancer in adult and aged patients of both sexes
Purpose: Colorectal cancer represents the second most common type of cancer in Serbia. Alteration of lipid metabolism begins early, and can represent a central hallmark in cancer evolution. Fatty acids have various important functions as building components of cell membranes, as signaling molecules in immune responses and also manage the general cancer signaling network. The purpose of this study was to investigate the difference of various fatty acids content between colorectal cancer and adjacent healthy intestinal tissue in adult and aged patients of both sexes. Methods: 52 subjects participated in this study. Healthy colon mucosa and tumor tissue samples were obtained from patients previously diagnosed with colorectal carcinoma. Simplified method of Berstad et al was used for direct transesterification of total lipids in tumor and healthy mucosa tissue samples and separations of the methyl esters was carried out using a gas chromatograph equipped with a split/ splitless injector and a flame ionization detector. Results: 18 0, 18 1 n7, 20 3, 20 4, 20 5, 22 4, 22 5 22 6, SFA, PUFA, n6, n3 and AA/EPA were significantly higher in tumor tissue. On the other hand, 18 1 n9, 18 2, 18 3 n3, MUFA, n6/ n3 were significantly higher in healthy tissue. Conclusions: Saturation index (SI) could be a valuable tool to delineate robust immune response and worse prognosis in patients with colorectal cancer. Our study demonstrated significant differences in fatty acid profiles between tumor tissue and healthy mucosa. Parameters, such as gender, age, stage and mucinous component didn't influence altered fatty acid content
Collagen type I alpha 1 gene polymorphism in premature ovarian failure
Introduction. Premature ovarian failure (POF) is characterized by amenorrhea, hypergonadotropism and hypoestrogenism in women bellow 40 years. Osteoporosis is one of the late complications of POF. Objective. To correlate collagen type I alpha1 (COLIA1) gene polymorphism with bone mineral density (BMD) in women with POF. Methods. We determined the COLIA1 genotypes SS, Ss, ss in 66 women with POF. Single nucleotide polymorphism (G to T substitution) within the Sp 1-binding site in the first intron of the COLIA1 gene was assessed by polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis. Bone mineral density (BMD) was measured at the lumbar spine region by dual X-ray absorptiometry. Statistics: Kruskal-Wallis ANOVA, Chisquare test, Spearman correlation test. Results. The relative distribution of COLIA1 genotype alleles was SS - 54.4%, Ss - 41.0% and ss - 4.5%. No significant differences were found between genotype groups in body mass index, age, duration of amenorrhea or BMD. A significant positive correlation was observed between BMI and parity. Conclusion. The COLIA1 gene is just one of many genes influencing bone characteristics. It may act as a marker for differences in bone quantity and quality, bone fragility and accelerated bone loss in older women. However, in young women with POF, COLIA1 cannot identify those at higher risk for osteoporosis.Uvod. Prevremena insuficijencija jajnika (PIJ) se odlikuje amenorejom, hipergonadotropizmom i hipoestrogenijom kod žena mlaÄih od 40 godina. Osteoporoza je kasna komplikacija ovog stanja. Cilja rada. Cilj istraživanja je bio da se uporede genski polimorfizam kolagena tip I alfa 1 (COLIA1) sa gustinom koÅ”tane mase kod žena sa PIJ. Metode rada. OdreÄivan je COLIA1 genotip SS, Ss i ss kod 66 žena sa PIJ pomoÄu eseja za reakciju lanÄanog umnožavanja DNK (engl. polymerase chain reaction - PCR). Polimorfizam jednog nukleotida (zamena G u T) u okviru Sp1 vezujuÄeg mesta u prvom intronu gena COLIA1 odreÄivan je primenom PCR, nakon Äega se pristupilo analizi konformacionog polimorfizma. Gustina koÅ”tane mase je merena na nivou lumbalnog dela kiÄme pomoÄu apsorpciometrije. Hormonske analize za folikulostimuliÅ”uÄi hormon, luteinizirajuÄi hormon, estradiol, prolaktin, progesteron i testosteron uraÄene su primenom metoda RIA. Pri statistiÄkoj obradi podataka koriÅ”Äeni su: Kraskal-Volisov (Kruskal-Wallis) ANOVA test, Ļ2-test i Spirmanov (Spearman) test korelacije. Rezultati. Relativna distribucija alela COLIA1 genotipa bila je: SS 54,4%, Ss 41,0% i ss 4,5%. Nije utvrÄena znaÄajna razlika izmeÄu grupa prema genotipu za gustinu koÅ”tane mase, starost ispitanica, period amenoreje ili indeks telesne mase žena. ZnaÄajna pozitivna korelacija je uoÄena za indeks telesne mase i paritet. ZakljuÄak. COLIA1 je samo jedan od mnogih gena koji utiÄu na karakteristike kosti. Kod žena starije životne dobi on može biti marker kvaliteta, kvantiteta i osetljivosti kosti. Kod mladih žena sa PIJ COLIA1 ne može da ukaže na one žene kod kojih postoji veÄi rizik za nastanak osteoporoze.Projekat ministarstva br. ON 17305
Supplementary data for the article: StojsavljeviÄ, A.; RovÄanin, M.; MikoviÄ, Ž.; PeroviÄ, M.; JeremiÄ, A.; ZeÄeviÄ, N.; ManojloviÄ, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.
Supplementary material for: [https://doi.org/10.1007/s11356-021-18190-y]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4879
Supplementary material for the article: MiloÅ”ev, M. Z.; JakovljeviÄ, K.; JoksoviÄ, M. D.; StanojkoviÄ, T.; MatiÄ, I. Z.; PeroviÄ, M.; TeÅ”iÄ, V.; Kanazir, S.; MladenoviÄ, M.; RodiÄ, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943ā952. https://doi.org/10.1111/cbdd.12920
Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221
Supplementary material for the article: MiloÅ”ev, M. Z.; JakovljeviÄ, K.; JoksoviÄ, M. D.; StanojkoviÄ, T.; MatiÄ, I. Z.; PeroviÄ, M.; TeÅ”iÄ, V.; Kanazir, S.; MladenoviÄ, M.; RodiÄ, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943ā952. https://doi.org/10.1111/cbdd.12920
Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221
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