Haemoglobin mass and running time trial performance after recombinant human erythropoietin administration in trained men

<p>Recombinant human erythropoietin (rHuEpo) increases haemoglobin mass (Hbmass) and maximal oxygen uptake (v˙ O2 max).</p> <p>Purpose: This study defined the time course of changes in Hbmass, v˙ O2 max as well as running time trial performance following 4 weeks of rHuEpo administration to determine whether the laboratory observations would translate into actual improvements in running performance in the field.</p> <p>Methods: 19 trained men received rHuEpo injections of 50 IUNkg21 body mass every two days for 4 weeks. Hbmass was determined weekly using the optimized carbon monoxide rebreathing method until 4 weeks after administration. v˙ O2 max and 3,000 m time trial performance were measured pre, post administration and at the end of the study.</p> <p>Results: Relative to baseline, running performance significantly improved by ,6% after administration (10:3061:07 min:sec vs. 11:0861:15 min:sec, p,0.001) and remained significantly enhanced by ,3% 4 weeks after administration (10:4661:13 min:sec, p,0.001), while v˙ O2 max was also significantly increased post administration (60.765.8 mLNmin21Nkg21 vs. 56.066.2 mLNmin21Nkg21, p,0.001) and remained significantly increased 4 weeks after rHuEpo (58.065.6 mLNmin21Nkg21, p = 0.021). Hbmass was significantly increased at the end of administration compared to baseline (15.261.5 gNkg21 vs. 12.761.2 gNkg21, p,0.001). The rate of decrease in Hbmass toward baseline values post rHuEpo was similar to that of the increase during administration (20.53 gNkg21Nwk21, 95% confidence interval (CI) (20.68, 20.38) vs. 0.54 gNkg21Nwk21, CI (0.46, 0.63)) but Hbmass was still significantly elevated 4 weeks after administration compared to baseline (13.761.1 gNkg21, p<0.001).</p> <p>Conclusion: Running performance was improved following 4 weeks of rHuEpo and remained elevated 4 weeks after administration compared to baseline. These field performance effects coincided with rHuEpo-induced elevated v˙ O2 max and Hbmass.</p&gt

Thermal imaging in the 3-5 micron range for precise localization of defects: Application on frescoes at the Sforza Castle

Infrared methods are of great importance in nondestructive testing of artworks, allowing a remote and wide-field imaging of interesting hidden features. Here we discuss a workflow based on thermal imaging in the mid infrared 3-5 micron range for the evaluation of subsurface defects in frescoes. Particular attention is payed to obtaining a high resolution (submillimetric) localization of the defects. The transfer of diagnostics techniques into real world applications, is discussed through the proof of concept of the proposed workflow on frescoes at the Sforza Castle (Milan, Italy)

Características produtivas de sorgo forrageiro em cultivos de segunda safra.

Evento online

Hydroxychloroquine drastically reduces immune activation in HIV-infected, antiretroviral therapy-treated immunologic nonresponders

Despite optimal suppression of HIV replication, restoration of CD4(+) T cells is not always achieved in antiretroviral therapy-treated individuals. Defective CD4 recovery in immunologic nonresponders is possibly associated with TLR-mediated immune activation driven by alterations of gut permeability. Hydroxychloroquine (HCQ) reduces endosomal TLR signaling; thus, we verified whether HCQ could dampen immune activation and be associated with an increase in CD4(+) T cells. To this end, we enrolled in a prospective study 20 HIV-infected immunologic nonresponders (CD4 count < 200 cells/mL or CD4 increase < 5% in the last 12 months) who received 400 mg/day HCQ for 6 months. HCQ had a notable impact on immune activation as shown by significant modifications of the following parameters: (1) reduced plasma lipopolysaccharide; (2) decreased TLR4-expressing CD14(+) cells, TLR4-mediated signal transduction, and mRNA synthesis; (3) reduced percentages of activated CD4(+) (CD4(+)/Ki67(+)) and CD14(+) (CD14(+)/CD69(+)) cells; (4) increased T-regulatory cells (Tregs), naive Tregs, and TLR4-expressing Tregs; (5) augmented plasmacytoid dendritic cells and reduced IFN alpha-secreting plasmacytoid dendritic cells; and (6) reduced IL-6 and TNF alpha production. HCQ-induced immune modulation was associated with increased percentages of circulating CD4(+) T cells and was mostly retained 2 months after therapy interruption. HCQ reduces lipopolysaccharide/TLR-mediated immune activation; this compound could be a useful immunomodulant in HIV-infected patients. This study is registered at EutraCT as 2009-012499-28 with study number HLS01/2009-1-16-03-2009. (Blood. 2011;118(12):3263-3272

Metodologias de mensuração do pH de silagens do sorgo forrageiro e sorgo biomassa.

Este trabalho comparou diferentes métodos de análise de pH, com amostras de silagem de sorgo forrageiro e sorgo biomassa em duas condições (frescas e congeladas). O experimento de campo foi desenvolvido na Embrapa Agrossilvipastoril em março de 2019, no município de Sinop-MT. Foi utilizado um delineamento experimental inteiramente casualizado, com cinco repetições. Para o sorgo biomassa, os tratamentos corresponderam a um fatorial 5x2, sendo cinco metodologias de avaliação do pH e duas condições de amostras. Para o sorgo forrageiro, os tratamentos corresponderam a um fatorial 4x2, sendo quatro metodologias de mensuração do pH e duas condições de amostras. Recomendamos a mensuração do pH logo após a abertura do silo para o sorgo forrageiro e sorgo biomassa. As metodologias propostas por Cherney e Cherney (2003) e por Kung Jr (1996) devem ser utilizadas com preferência na análise de silagens de sorgo biomassa

PLATAFORMA ELÉTRICA RESOLVE DESAFIO DE MOBILIDADE PARA CADEIRANTES

Artigo em PD

Ensino de língua materna: dificuldades e necessidades formativas apontadas por professores na Educação Fundamental

Resumo Há muitos desafios postos ao professor para o ensino de língua materna na perspectiva da alfabetização e do letramento como práticas sociais. No caso específico do município de Presidente Prudente, torna-se essencial compreender como ocorre o processo de formação continuada dos professores atuantes nos anos iniciais do Ensino Fundamental para que sejam pensadas ações formativas, tanto no âmbito do sistema municipal quanto nas escolas, que enfatizem o ensino de língua materna. Nesse sentido, este artigo objetiva refletir sobre a formação do professor dos anos iniciais do Ensino Fundamental, a partir das dificuldades e das necessidades formativas apresentadas pelos docentes com relação ao ensino de língua materna. Trata-se de uma pesquisa de base qualitativa, financiada pelo CNPq, por meio da qual analisamos os dados referentes a 22 escolas vinculadas à Secretaria de Educação do município, no que diz respeito às respostas dos professores emitidas por meio de questionários. Para este artigo, estabelecemos um recorte com o intuito de apresentar os dados relativos às respostas a duas questões dentre as que compuseram o questionário respondido pelos professores: uma referente às dificuldades que enfrentam para o ensino de língua materna e outra que diz respeito às necessidades formativas para este ensino. O que os participantes da pesquisa apontam como principais dificuldades relacionadas ao ensino de língua materna comparecem também no que evidenciam como necessidades formativas