Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control

Processing and maturation of precursor RNA species is coupled to RNA polymerase II transcription. Co-transcriptional RNA processing helps to ensure efficient and proper capping, splicing, and 3' end processing of different RNA species to help ensure quality control of the transcriptome. Many improperly processed transcripts are not exported from the nucleus, are restricted to the site of transcription, and are in some cases degraded, which helps to limit any possibility of aberrant RNA causing harm to cellular health. These critical quality control pathways are regulated by the highly dynamic protein-protein interaction network at the site of transcription. Recent work has further revealed the extent to which the processes of transcription and RNA processing and quality control are integrated, and how critically their coupling relies upon the dynamic protein interactions that take place co-transcriptionally. This review focuses specifically on the intricate balance between 3' end processing and RNA decay during transcription termination. This article is categorized under: RNA Turnover and Surveillance > Turnover/Surveillance Mechanisms RNA Processing > 3' End Processing RNA Processing > Splicing Mechanisms RNA Processing > Capping and 5' End Modifications

Expert advice and political choice in constructing European banking union

International actors promoted the transfer of regulatory authority and financial resources from national governments to the European Union (EU) in the context of establishing the prerequisites for financial stability in Europe through banking union. It was supplied, however, by a political process that kept significant resources in resolution and deposit insurance largely in national hands. This article examines the politics behind those decisions, and how the hybrid of European and national competences affects bank regulation and financial stability in the EU. It concludes that the tension between strong EU supervisory powers and weak capacity to deal with insolvent institutions will persist

Quantitative diffusion tensor imaging detects dopaminergic neuronal degeneration in a murine model of Parkinson\u27s disease.

Early diagnosis of Parkinson\u27s disease (PD) is required to improve therapeutic responses. Indeed, a clinical diagnosis of resting tremor, rigidity, movement and postural deficiencies usually reflect \u3e50% loss of the nigrostriatal system in disease. In a step to address this, quantitative diffusion tensor magnetic resonance imaging (DTI) was used to assess nigrostriatal degeneration in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxication model of dopaminergic nigral degeneration. We now demonstrate increased average diffusion (p\u3c0.005) and decreased fractional anisotropy (p\u3c0.03) in the substantia nigra (SN) of 5- to 7-day MPTP-treated animals when compared to saline controls. Transverse diffusivity demonstrated the most significant differences (p \u3c or = 0.002) and correlated with the numbers of SN dopaminergic neurons (r=-0.75, p=0.012). No differences were found in the striatum, corpus callosum, cerebral cortex, or ventricles. These results demonstrate that DTI may be used as a surrogate biomarker of nigral dopaminergic neuronal degeneration

Measuring health inequality among children in developing countries: does the choice of the indicator of economic status matter?

Background Currently, poor-rich inequalities in health in developing countries receive a lot of attention from both researchers and policy makers. Since measuring economic status in developing countries is often problematic, different indicators of wealth are used in different studies. Until now, there is a lack of evidence on the extent to which the use of different measures of economic status affects the observed magnitude of health inequalities. Methods This paper provides this empirical evidence for 10 developing countries, using the Demographic and Health Surveys data-set. We compared the World Bank asset index to three alternative wealth indices, all based on household assets. Under-5 mortality and measles immunisation coverage were the health outcomes studied. Poor-rich inequalities in under-5 mortality and measles immunisation coverage were measured using the Relative Index of Inequality. Results Comparing the World Bank index to the alternative indices, we found that (1) the relative position of households in the national wealth hierarchy varied to an important extent with the asset index used, (2) observed poor-rich inequalities in under-5 mortality and immunisation coverage often changed, in some cases to an important extent, and that (3) the size and direction of this change varied per country, index, and health indicator. Conclusion Researchers and policy makers should be aware that the choice of the measure of economic status influences the observed magnitude of health inequalities, and that differences in health inequalities between countries or time periods, may be an artefact of different wealth measures used

The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae

The phosphatase Rtr1 has been implicated in dephosphorylation of the RNA Polymerase II (RNAPII) C-terminal domain (CTD) during transcription elongation and in regulation of nuclear import of RNAPII. Although it has been shown that Rtr1 interacts with RNAPII in yeast and humans, the specific mechanisms that underlie Rtr1 recruitment to RNAPII have not been elucidated. To address this, we have performed an in-depth proteomic analysis of Rtr1 interacting proteins in yeast. Our studies revealed that hyperphosphorylated RNAPII is the primary interacting partner for Rtr1. To extend these findings, we performed quantitative proteomic analyses of Rtr1 interactions in yeast strains deleted for CTK1, the gene encoding the catalytic subunit of the CTD kinase I (CTDK-I) complex. Interestingly, we found that the interaction between Rtr1 and RNAPII is decreased in ctk1Δ strains. We hypothesize that serine-2 CTD phosphorylation is required for Rtr1 recruitment to RNAPII during transcription elongation

Unified View of Scaling Laws for River Networks

Scaling laws that describe the structure of river networks are shown to follow from three simple assumptions. These assumptions are: (1) river networks are structurally self-similar, (2) single channels are self-affine, and (3) overland flow into channels occurs over a characteristic distance (drainage density is uniform). We obtain a complete set of scaling relations connecting the exponents of these scaling laws and find that only two of these exponents are independent. We further demonstrate that the two predominant descriptions of network structure (Tokunaga's law and Horton's laws) are equivalent in the case of landscapes with uniform drainage density. The results are tested with data from both real landscapes and a special class of random networks.Comment: 14 pages, 9 figures, 4 tables (converted to Revtex4, PRE ref added

Exploring Strategies of American Small Business Owners in United Arab Emirates

The International Trade Administration reported that 70% of worldwide revenue for American businesses comes from consumers in emergent markets such as India, the Middle East, and Northern Africa. However, the United Arab Emirates (UAE) is a country that many American small business owners are not operating in and capitalizing on the financial opportunities that are available. The purpose of this multiple case study was to explore strategies American small business owners use in successful international entrepreneurship for increased revenue and operational longevity. The study population consisted of 5 American small business entrepreneurs who had succeeded operating in the UAE for at least 5 years. The resource-based view theory was the conceptual framework that grounded this study. Data collection included semistructured interviews and a review of company documents and government records. Data analysis involved coding, word clustering, and the use of qualitative data analytical software to identify patterns. Member checking of interview responses helped to strengthen the integrity of the findings. The use of methodical triangulation enabled a rich analysis and an enhanced trustworthiness of the results. The 4 key themes that emerged from the data analysis were strategic planning, understanding local culture, building relationships, and using digital platforms. Small business owners could use the key themes for the development of international entrepreneurial strategies. The findings from this study and the knowledge generated may influence positive social change by increasing local employment, improving economic conditions, and promoting the development of international business operations for American entrepreneurs

Annual net snow accumulation over southern Greenland from 1975 to 1998

As part of NASA's Program for Arctic Regional Climate Assessment (PARCA), extensive ice core measurements of annual net water-equivalent accumulation have been made recently around the southern Greenland ice sheet. Analysis of these measurements demonstrates that annual and seasonal accumulation patterns are sometimes regional, with temporal variability in accumulation correlated over large areas. Using this unique, widely distributed set of contemporaneous accumulation measurements, as well as available previously published observations, we developed maps of annual net snow accumulation south of �73° N for each year from 1975 to 1998. Here net snow accumulation is defined as snow accumulation minus ablation. In order to achieve a more consistent spatial distibution of core measurements for each of the 24 years in the study period, some of the observed records were extrapolated up to 5 years using empirical relationships between monthly precipitation measured at coastal stations and the observed ice core net accumulation records. Initial comparisons between the maps of annual net snow accumulation and similar maps of net accumulation derived from meteorological model simulations show excellent agreement in the temporal variability of accumulation, although significant differences in the magnitude of accumulation remain. Both measurements and model simulations indicate that annual net accumulation, averaged over all higher-elevation regions (above 2000 m) of the southern ice sheet, varies significantly from one year to the next. The maximum year-to-year change during the 24-year study period occurred between calendar years 1995 and 1996, when the average annual net snow accumulation increased by 101 and 172 kg m-2 yr-1, or 37 and 57, for observations and model simulations, respectively. Taken alone, this 1-year change in average net snow accumulation corresponds to a drop in sea level of �0.16 and �0.28 mm yr-1. Copyright 2001 by the American Geophysical Union