52 research outputs found
Assisted fertilization outcome assesment using ovarian reserve tests
Uvod: Optimizacija i individualizacija kontrolisane ovarijalne hiperstimulacije
postaje sve značajnija, pa se brojna ispitivanja bave identifikacijom kliničkih
parametara koji mogu da utiču na ovarijalni odgovor, kao i da povećaju efikasnost i
bezbednost primenjene stimulacije. Cilj ovog istraživanja je ispitivanje mogućnosti
predviđanja ovarijalnog odgovora i ishoda asistirane fertilizacije na osnovu
biomarkera funkcionalne ovarijalne rezerve, kao i identifikacija onog koji bi imao
preciznost nezavisnu od primenjenog protokola stimulacije.
Metodologija:Prospektivna kohortna studija je obuhvatila 363 pacijentkinje u
postupku asistirane fertilizacije u okviru Nacionalnog programa na Klinici za
ginekologiju i akušerstvo Kliničkog centra Srbije. Svima su određeni starost, indeks
telesne mase, uzrok i vrsta infertiliteta. Broj antralnih folikula i vrednosti AMH,
Inhibina B, FSH, LH, estradiola, progesterona i testosterona u serumu su mereni
drugog dana ciklusa pre započinjanja stimulacije. Broj i kvalitet dobijenih oocita i
embriona i stope biohemijskih i kliničkih trudnoća su korišćeni za evaluaciju
prediktivne mogućnosti ovih biomarkera.
Rezultati: Pokazano je da su starost ispitanica, broj antralnih folikula, vrednost AMH
i odnos FSH/LH prediktori broja dobijenih oocita kao i stope trudnoća. Dodatno, broj
antralnih folikula je bio najvažniji paramtar koji je uticao na broj dobijenih oocita i
stopu trudnoća, nezavisno od primenjenog protokola stimulacije..
Tourism Policy in Montenegro - Current Situation and Future Challenges
Located on the coast of the Adriatic Sea, Montenegro has been an important receptive market for decades. In accordance with strategic documents, tourism has been identified as а leading economic activity in Montenegro. Although it has а diversified tourist offer, the emphasis has been put on coastal tourism. Adapting to changes in the market, the offer has been transformed from mass, social vacations towards individual products intended to premium customers. Therefore, the aim of this paper is to evaluate the effects of the current tourism policy defined Ьу Montenegro tourism development strategy to 2020. The analysis includes the key indicatшs of the tourism industry. The results have shown that seasonality is the major obstacle for the balanced tourism development. Hence, all eff orts should Ье directed towards creating products that exceed classic "sun, sand, and sea" tourism model
Insulin resistance and inflammatory response following abdominal surgery
Pojava prolazne i vremenski ograničene insulinske rezistencije tokom hirurškog
zahvata je povezana sa vrstom operacije kao i sa veličinom povrede tkiva. Cilj naše
studije je bio da procenimo insulinsku rezistenciju merenu matematičkim modelom
HOMA-IR i faktore inflamacije IL-6, TNF-α i CRP-a pre i posle laparoskopske i
otvorene holecistektomije i otvorene rekonstrukcije ingvinalnih kila tokom
sedmodnevenog praćenja, kao i ispitivanje prediktivne vrednosti preoperativnog odnosa
neutrofila i limfocita u proceni težine bolesti kod bolesnika sa holecistitisom i njegove
eventualne povezanosti sa stepenom insulinske senzitivnosti..
Metode: Istraživanje koje je sprovedeno po tipu ne-randomizovane prospektivne studije
obuhvatilo je 192 bolesnika sa kamenom u žučnoj kesi (operisanih primenom otvorene
ili laparoskopske holecistektomje) ili sa ingvinalnom kilom (operisanih otvoreno
metodom) koji su operisani na Klinici za Urgentnu hirurgiju Urgentnog centra
Kliničkog centra Srbije u toku sprovodjenja studije. Glukoza, insulin, CRP, IL-6, TNF-
α, Le, Ly i Ne su određivani pre operacije (0 dan) i 1., 3. i 7. dan posle operacije.
Rezultati: Veće vrednosti indeksa HOMA-IR su nađene kod pacijenata nakon otvorene
operacije žučne kese u poređenju sa laparoskopskom operacijom u trećem
postoperativnom danu. (p=0,036). HOMA-IR je bio značajno veći nakon otvorene
rekonstrukcije kila u poređenju sa grupom nakon laparoskopske operacije žučne kese
prvog postoperativnog dana (p=0,045). Vrednost CRP je bila značajno veća u prvom
postoperativnom danu kod bolesnika nakon otvorene operacije žučne kese u odnosu na
laparoskopsku operaciju (p=0,046). IL-6 se značajno povećao 1. i 3.dana u odnosu na
0.dan (p<0,0005) u grupi nakon laparoskopske operacije žučne kese i nakon otvorene
rekonstrukcije kile. Povećanje IL-6 je bilo statistički značajno veće 1.postoperativnog
dana nakon otvorene operacije kile u odnosu na laparoskopsku operaciju žučne kese
(p=0,044). Pozitivna korelacija između CRP i HOMA-IR (r=0,46; p=0,025) i između
IL-6 i HOMA-IR (r=0,44; p=0,030) postojala je u grupi sa otvorenom rekonstrukcijom
kila prvog dana...The development of transient and temporal insulin resistance after
surgical intervention is associated with the type and magnitude of operation and tissue
injury. The aim of our study was to evaluate insulin resistance assesed by homeostatic
model assessment of insulin resistance (HOMA-IR) and inflammation factors IL-6,
TNF-α and CRP before and after laparoscopic and open cholecystectomy and open
hernia repair during seven days follow-up, as well as the predictive value of
preoperative neutrophil-to-lymphocyte ratio (NLR) to asses the severity of cholecystitis
and its possible association with the degree of insulin resistance.
Methods: a non-randomized prospective study included 192 patients with stones in
gallblader (treated with laparoscopic or open cholecystectomy) and inguinal hernia
repair treated with open method. Study was conducted at Clinic for emergency surgery,
Emergency center, Clinical Center of Serbia during the course of the investigation.
Results: Significantly higher values of HOMA-IR were found in patients with open
cholecystectomy in comparison with patients with laparoscopic cholecystectomy on
third postoperative day (p=0.036). HOMA-IR was significantly higher after open hernia
repair in comparison with laparoscopic cholecystectomy at first postoperative day
(p=0.045). CRP was significantly higher in patients after open cholecystectomy in
comparison with patients with laparoscopic cholecystectomy, at day one (p=0.046).
Statistically higher values of IL-6 were found at day 1 and 3 in comparison with day 0
in group with laparoscopic cholecystectomy and in group with open hernia repair
(p<0.0005). IL-6 was significantly higher at day 1 after open hernia repair in
comparison with laparoscopic cholecystectomy (p=0.044). Positive correlation between
CRP and HOMA-IR (r=0.46; p=0.025) and between IL-6 and HOMA-IR (r=0.44;
p=0.030) was found at day one in group with open hernia repair. More severe
cholecystitis was found among patients in high NLR group (p<0.0001) and higher level
of CRP and white blood cells counts (WBC) (p<0.0001). The NLR of 4.18 could predict
severe cholecystits. Significant correlation between preoperative NLR and HOMA-IR
values at first postoperative day was established (r=0.254, p=0.030)..
AUTOMATIC DETERMINATION OF FILTER COEFFICIENTS FOR LOCAL CONTRAST ENHANCEMENT
This study proposes an algorithm whose main advantage is in enabling the automatic determination of non-linear homomorphic filter coefficients used for local contrast enhancement in digital image processing. The presented algorithm is tested in a real production environment. The obtained results are compared with relevant examples in literature, showing the advantages of the achieved results or a relatively high level of their correspondence with reference results. The proposed procedure can be used for various applications in mechatronics, robotics and automatized production systems
Effect of acute exercise on serum growth hormone and fatty acid levels in elite male water polo players
The aim of study was to estimate the effect of acute exercise on serum growth
hormone (GH) and fatty acid (FFA) levels in elite water polo players. Twelve
male water polo players (20.50 ± 2.02 years) and eleven non-athletic male
subjects (20.55 ± 1.04 years) participated in this study. In order to
determine GH and FFA responses to acute exercise, a treadmill-running test
was performed following an incremental protocol. Pre-exercise blood samples
for both athletes and non-athletes were taken at 9 AM. Post-exercise samples
were taken immediately after and 30 min after the treadmill running test.
Water polo players had significantly lower baseline values of serum GH
concentration compared to controls, whereas serum FFA concentration was
significantly higher in water polo players compared to controls (p<0.01;
p<0.05, respectively). In both groups, concentration of GH was significantly
higher immediately after and after the 30-min of recovery compared to
baseline levels (p<0.05). In water polo players, the concentration of FFA was
significantly decreased immediately after and after the 30-min of recovery
compared to baseline levels (p<0.05). No significant response to maximal
exercise test was observed in the control group for serum FFA concentration.
Our research indicates that acute exercise resulted in a significant increase
in serum GH and reduction in fatty acid levels in elite water polo players.
[Projekat Ministarstva nauke Republike Srbije, br. OI 175067
Collagen type I alpha 1 gene polymorphism in premature ovarian failure
Introduction. Premature ovarian failure (POF) is characterized by amenorrhea, hypergonadotropism and hypoestrogenism in women bellow 40 years. Osteoporosis is one of the late complications of POF. Objective. To correlate collagen type I alpha1 (COLIA1) gene polymorphism with bone mineral density (BMD) in women with POF. Methods. We determined the COLIA1 genotypes SS, Ss, ss in 66 women with POF. Single nucleotide polymorphism (G to T substitution) within the Sp 1-binding site in the first intron of the COLIA1 gene was assessed by polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis. Bone mineral density (BMD) was measured at the lumbar spine region by dual X-ray absorptiometry. Statistics: Kruskal-Wallis ANOVA, Chisquare test, Spearman correlation test. Results. The relative distribution of COLIA1 genotype alleles was SS - 54.4%, Ss - 41.0% and ss - 4.5%. No significant differences were found between genotype groups in body mass index, age, duration of amenorrhea or BMD. A significant positive correlation was observed between BMI and parity. Conclusion. The COLIA1 gene is just one of many genes influencing bone characteristics. It may act as a marker for differences in bone quantity and quality, bone fragility and accelerated bone loss in older women. However, in young women with POF, COLIA1 cannot identify those at higher risk for osteoporosis.Uvod. Prevremena insuficijencija jajnika (PIJ) se odlikuje amenorejom, hipergonadotropizmom i hipoestrogenijom kod žena mlađih od 40 godina. Osteoporoza je kasna komplikacija ovog stanja. Cilja rada. Cilj istraživanja je bio da se uporede genski polimorfizam kolagena tip I alfa 1 (COLIA1) sa gustinom koštane mase kod žena sa PIJ. Metode rada. Određivan je COLIA1 genotip SS, Ss i ss kod 66 žena sa PIJ pomoću eseja za reakciju lančanog umnožavanja DNK (engl. polymerase chain reaction - PCR). Polimorfizam jednog nukleotida (zamena G u T) u okviru Sp1 vezujućeg mesta u prvom intronu gena COLIA1 određivan je primenom PCR, nakon čega se pristupilo analizi konformacionog polimorfizma. Gustina koštane mase je merena na nivou lumbalnog dela kičme pomoću apsorpciometrije. Hormonske analize za folikulostimulišući hormon, luteinizirajući hormon, estradiol, prolaktin, progesteron i testosteron urađene su primenom metoda RIA. Pri statističkoj obradi podataka korišćeni su: Kraskal-Volisov (Kruskal-Wallis) ANOVA test, χ2-test i Spirmanov (Spearman) test korelacije. Rezultati. Relativna distribucija alela COLIA1 genotipa bila je: SS 54,4%, Ss 41,0% i ss 4,5%. Nije utvrđena značajna razlika između grupa prema genotipu za gustinu koštane mase, starost ispitanica, period amenoreje ili indeks telesne mase žena. Značajna pozitivna korelacija je uočena za indeks telesne mase i paritet. Zaključak. COLIA1 je samo jedan od mnogih gena koji utiču na karakteristike kosti. Kod žena starije životne dobi on može biti marker kvaliteta, kvantiteta i osetljivosti kosti. Kod mladih žena sa PIJ COLIA1 ne može da ukaže na one žene kod kojih postoji veći rizik za nastanak osteoporoze.Projekat ministarstva br. ON 17305
Rosemary essential oils as a promising source of bioactive compounds: Chemical composition, thermal properties, biological activity, and gastronomical perspectives
Rosemary (Rosmarinus officinalis L.) is a plant worldwide cultivated mainly for essential oils, extracts, and as a spice. Up-to-date results showed diversity in composition of the essential oils, which may influence their quality, biological activity, and thermal properties. Therefore, the aim of this study was to investigate the chemical composition, antimicrobial activity, and thermal properties of the rosemary essential oils originating from Serbia and Russia. Additionally, oils were added to the sunflower oils in order to investigate possible antioxidant activity during the frying. Investigation of the chemical profile marked α-pinene, eucalyptol, and camphor as the most abundant compounds in both oils. However, overall composition influenced in such manner that Russian oil showed significantly higher antimicrobial activity, while Serbian oil proved to be better antioxidant agent in case of frying of sunflower oil. This would significantly influence possible application of the oils, which could be used as an antioxidant agent for extension of the food shelf life, or antimicrobial agent for protection against different microbial strains
Collagen type I alpha 1 gene polymorphism in premature ovarian failure
Introduction. Premature ovarian failure (POF) is characterized by amenorrhea, hypergonadotropism and hypoestrogenism in women bellow 40 years. Osteoporosis is one of the late complications of POF. Objective. To correlate collagen type I alpha1 (COLIA1) gene polymorphism with bone mineral density (BMD) in women with POF. Methods. We determined the COLIA1 genotypes SS, Ss, ss in 66 women with POF. Single nucleotide polymorphism (G to T substitution) within the Sp 1-binding site in the first intron of the COLIA1 gene was assessed by polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis. Bone mineral density (BMD) was measured at the lumbar spine region by dual X-ray absorptiometry. Statistics: Kruskal-Wallis ANOVA, Chisquare test, Spearman correlation test. Results. The relative distribution of COLIA1 genotype alleles was SS - 54.4%, Ss - 41.0% and ss - 4.5%. No significant differences were found between genotype groups in body mass index, age, duration of amenorrhea or BMD. A significant positive correlation was observed between BMI and parity. Conclusion. The COLIA1 gene is just one of many genes influencing bone characteristics. It may act as a marker for differences in bone quantity and quality, bone fragility and accelerated bone loss in older women. However, in young women with POF, COLIA1 cannot identify those at higher risk for osteoporosis.Uvod. Prevremena insuficijencija jajnika (PIJ) se odlikuje amenorejom, hipergonadotropizmom i hipoestrogenijom kod žena mlađih od 40 godina. Osteoporoza je kasna komplikacija ovog stanja. Cilja rada. Cilj istraživanja je bio da se uporede genski polimorfizam kolagena tip I alfa 1 (COLIA1) sa gustinom koštane mase kod žena sa PIJ. Metode rada. Određivan je COLIA1 genotip SS, Ss i ss kod 66 žena sa PIJ pomoću eseja za reakciju lančanog umnožavanja DNK (engl. polymerase chain reaction - PCR). Polimorfizam jednog nukleotida (zamena G u T) u okviru Sp1 vezujućeg mesta u prvom intronu gena COLIA1 određivan je primenom PCR, nakon čega se pristupilo analizi konformacionog polimorfizma. Gustina koštane mase je merena na nivou lumbalnog dela kičme pomoću apsorpciometrije. Hormonske analize za folikulostimulišući hormon, luteinizirajući hormon, estradiol, prolaktin, progesteron i testosteron urađene su primenom metoda RIA. Pri statističkoj obradi podataka korišćeni su: Kraskal-Volisov (Kruskal-Wallis) ANOVA test, χ2-test i Spirmanov (Spearman) test korelacije. Rezultati. Relativna distribucija alela COLIA1 genotipa bila je: SS 54,4%, Ss 41,0% i ss 4,5%. Nije utvrđena značajna razlika između grupa prema genotipu za gustinu koštane mase, starost ispitanica, period amenoreje ili indeks telesne mase žena. Značajna pozitivna korelacija je uočena za indeks telesne mase i paritet. Zaključak. COLIA1 je samo jedan od mnogih gena koji utiču na karakteristike kosti. Kod žena starije životne dobi on može biti marker kvaliteta, kvantiteta i osetljivosti kosti. Kod mladih žena sa PIJ COLIA1 ne može da ukaže na one žene kod kojih postoji veći rizik za nastanak osteoporoze.Projekat ministarstva br. ON 17305
Application of Landsat-derived NDVI in monitoring and assessment of vegetation cover changes in Central Serbia
This paper evaluates the application of the Normalised Difference Vegetation Index (NDVI) in the monitoring and assessment of temporal vegetation cover changes (from 2006 to 2014) in three municipalities of Central Serbia: Topola, Jagodina and Kuršumlija. Additionally, special focus is placed on the analysis of the forest areas and the possible use of NDVI in the forest management sector. Results of the NDVI applied through Idrisi software identify all vegetation cover types and their typical values for presented case studies and observed periods. Obtained results for Serbian case studies indicate two major observations outlined for the investigated period. It was noticed that vegetation cover is experiencing a certain decrease, and that certain discrepancies exists between the NDVI and official forest area statistics for certain municipalities. The study outlines the positive outcomes of the applied remote sensing techniques, especially for southern Serbian municipalities where illegal logging activities are pronounced. Hence, this method proved very promising for countries performing national forest inventories, such as Serbia, providing local forest managers with several essential up-to-date information about vegetation cover changes on an annual basis. © 2019 Carpathian Journal of Earth and Environmental Sciences
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