1,430 research outputs found
Splice variants of Enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy
Aims Proteins with a PDZ (for PSD-95, DLG, ZO-1) and one to three LIM (for Lin11, Isl-1, Mec-3) domains are scaffolding sarcomeric and cytoskeletal elements that form structured muscle fibres and provide for the link to intracellular signalling by selectively associating protein kinases, ion channels, and transcription factors with the mechanical stress-strain sensors. Enigma homolog (ENH) is a PDZ-LIM protein with four splice variants: ENH1 with an N-terminal PDZ domain and three C-terminal LIM domains and ENH2, ENH3, and ENH4 without LIM domains. We addressed the functional role of ENH alternative splicing. Methods and results We studied the expression of the four ENH isoforms in the heart during development and in a mouse model of heart hypertrophy. All four isoforms are expressed in the heart but the pattern of expression is clearly different between embryonic, neonatal, and adult stages. ENH1 appears as the embryonic isoform, whereas ENH2, ENH3, and ENH4 are predominant in adult heart. Moreover, alternative splicing of ENH was changed following induction of heart hypertrophy, producing an ENH isoform pattern similar to that of neonatal heart. Next, we tested a possible causal role of ENH1 and ENH4 in the development of cardiac hypertrophy. When overexpressed in rat neonatal cardiomyocytes, ENH1 promoted the expression of hypertrophy markers and increased cell volume, whereas, on the contrary, ENH4 overexpression prevented these changes. Conclusion Antagonistic splice variants of ENH may play a central role in the adaptive changes of the link between mechanical stress-sensing and signalling occurring during embryonic development and/or heart hypertroph
Study of the mechanical behavior of asphalt mixtures using fractional rheology to model their viscoelasticity
This study focuses on the mechanical behavior of asphalt mixtures composed of aggregate particles attached with an asphalt binder. Asphalt mixtures are viscoelastic composite materials widely used in the construction of pavement layers. The modelling of such materials is currently done using the Burgers model. However, this model is limited when explaining some of the viscoelastic phenomena of an asphalt mixture, mainly because the Burgers model was developed for a single material with a dual nature. This work presents a new approach that provides a more appropriate framework for studying asphalt mixtures. The model assumes an aggregate particle enclosed by an asphalt material. Viscoelastic equations were developed using derivatives of fractional order. Then, the creep, recovery, and relaxation phenomena in an asphalt mixture were analyzed using the new model. Unlike the Burgers model, the new model can predict the elastic jump observed at the beginning of the creep modulus. Thus, the new model seems to describe better those practical cases of asphalt mixtures used in the construction of pavement layers. The new model can be used to modify the properties of the binder for designing optimized and more resistant asphalt mixtures
Modeling relationships between calving traits: a comparison between standard and recursive mixed models
<p>Abstract</p> <p>Background</p> <p>The use of structural equation models for the analysis of recursive and simultaneous relationships between phenotypes has become more popular recently. The aim of this paper is to illustrate how these models can be applied in animal breeding to achieve parameterizations of different levels of complexity and, more specifically, to model phenotypic recursion between three calving traits: gestation length (GL), calving difficulty (CD) and stillbirth (SB). All recursive models considered here postulate heterogeneous recursive relationships between GL and liabilities to CD and SB, and between liability to CD and liability to SB, depending on categories of GL phenotype.</p> <p>Methods</p> <p>Four models were compared in terms of goodness of fit and predictive ability: 1) standard mixed model (SMM), a model with unstructured (co)variance matrices; 2) recursive mixed model 1 (RMM1), assuming that residual correlations are due to the recursive relationships between phenotypes; 3) RMM2, assuming that correlations between residuals and contemporary groups are due to recursive relationships between phenotypes; and 4) RMM3, postulating that the correlations between genetic effects, contemporary groups and residuals are due to recursive relationships between phenotypes.</p> <p>Results</p> <p>For all the RMM considered, the estimates of the structural coefficients were similar. Results revealed a nonlinear relationship between GL and the liabilities both to CD and to SB, and a linear relationship between the liabilities to CD and SB.</p> <p>Differences in terms of goodness of fit and predictive ability of the models considered were negligible, suggesting that RMM3 is plausible.</p> <p>Conclusions</p> <p>The applications examined in this study suggest the plausibility of a nonlinear recursive effect from GL onto CD and SB. Also, the fact that the most restrictive model RMM3, which assumes that the only cause of correlation is phenotypic recursion, performs as well as the others indicates that the phenotypic recursion may be an important cause of the observed patterns of genetic and environmental correlations.</p
"Meaning" as a sociological concept: A review of the modeling, mapping, and simulation of the communication of knowledge and meaning
The development of discursive knowledge presumes the communication of meaning
as analytically different from the communication of information. Knowledge can
then be considered as a meaning which makes a difference. Whereas the
communication of information is studied in the information sciences and
scientometrics, the communication of meaning has been central to Luhmann's
attempts to make the theory of autopoiesis relevant for sociology. Analytical
techniques such as semantic maps and the simulation of anticipatory systems
enable us to operationalize the distinctions which Luhmann proposed as relevant
to the elaboration of Husserl's "horizons of meaning" in empirical research:
interactions among communications, the organization of meaning in
instantiations, and the self-organization of interhuman communication in terms
of symbolically generalized media such as truth, love, and power. Horizons of
meaning, however, remain uncertain orders of expectations, and one should
caution against reification from the meta-biological perspective of systems
theory
Improving Clonal Colony Optimization to Evolve Robust Solutions
In this article we work with a recently introduced metaheuristic for robust optimization, inspired by the structure and behavior of biologic clonal colonies. We propose some improvements to increase their exploration and the exploitation capabilities. Our approach is compared to other robust optimization techniques, focusing in how the population is managed during the search
Relational Quantum Mechanics
I suggest that the common unease with taking quantum mechanics as a
fundamental description of nature (the "measurement problem") could derive from
the use of an incorrect notion, as the unease with the Lorentz transformations
before Einstein derived from the notion of observer-independent time. I suggest
that this incorrect notion is the notion of observer-independent state of a
system (or observer-independent values of physical quantities). I reformulate
the problem of the "interpretation of quantum mechanics" as the problem of
deriving the formalism from a few simple physical postulates. I consider a
reformulation of quantum mechanics in terms of information theory. All systems
are assumed to be equivalent, there is no observer-observed distinction, and
the theory describes only the information that systems have about each other;
nevertheless, the theory is complete.Comment: Substantially revised version. LaTeX fil
The Non-linear Dynamics of Meaning-Processing in Social Systems
Social order cannot be considered as a stable phenomenon because it contains
an order of reproduced expectations. When the expectations operate upon one
another, they generate a non-linear dynamics that processes meaning. Specific
meaning can be stabilized, for example, in social institutions, but all meaning
arises from a horizon of possible meanings. Using Luhmann's (1984) social
systems theory and Rosen's (1985) theory of anticipatory systems, I submit
equations for modeling the processing of meaning in inter-human communication.
First, a self-referential system can use a model of itself for the
anticipation. Under the condition of functional differentiation, the social
system can be expected to entertain a set of models; each model can also
contain a model of the other models. Two anticipatory mechanisms are then
possible: one transversal between the models, and a longitudinal one providing
the modeled systems with meaning from the perspective of hindsight. A system
containing two anticipatory mechanisms can become hyper-incursive. Without
making decisions, however, a hyper-incursive system would be overloaded with
uncertainty. Under this pressure, informed decisions tend to replace the
"natural preferences" of agents and an order of cultural expectations can
increasingly be shaped
Biological and methodological factors affecting VO2max response variability to endurance training and the influence of exercise intensity prescription
© 2021 The Authors. Experimental Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/Changes in cardiorespiratory fitness (CRF) in response to endurance training (ET) exhibit large variations, possibly due to a multitude of biological and methodological factors. It is acknowledged that ~20% of individuals may not achieve meaningful increases in CRF in response to ET. Genetics, the most potent biological contributor, has been shown to explain ~50% of response variability, whilst age, sex, and baseline CRF appear to explain a smaller proportion. Methodological factors represent the characteristics of the ET itself including the type, volume, and intensity of exercise, as well as the method used to prescribe and control exercise intensity. Notably, methodological factors are modifiable and, upon manipulation, alter response rates to ET, eliciting increases in CRF regardless of an individual’s biological predisposition. Particularly, prescribing exercise intensity relative to a physiological threshold (e.g. ventilatory threshold) is shown to increase CRF response rates compared to when intensity is anchored relative to a maximum physiological value (e.g. maximum heart rate). It is, however, uncertain whether the increased response rates are primarily attributable to reduced response variability, greater mean changes in CRF, or both. Future research is warranted to elucidate whether more homogenous chronic adaptations manifest over time among individuals, as a result of exposure to more homogenous exercise stimuli elicited by threshold-based practices.Peer reviewe
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