Comprometimento de estruturas encefálicas não hipotalâmicas na moléstia de Hand-Schüller-Christian: relato de caso e revisão da literatura

A case of Hand-Schüller-Christian disease which presented a pyramidal syndrome besides elements of the classical triad and concentric retraction of the visual field is reported. The rare involvement of non-hypothalamic encephalic strutures in that disease is emphasized together with the favourable effect of corticotherapy and radiotherapy.É relatado um caso de moléstia de Hand-Schüller-Christian que, além de elementos da tríade clássica e retração concêntrica do campo visual, apresentava uma síndrome piramidal. A raridade do comprometimento de estruturas encefálicas não hipotalâmicas nessa moléstia é realçada. É enfatizada a ação benéfica da corticoterapia e radioterapia observada no caso.Escola Paulista de Medicina Departamento de Neurologia e NeurocirurgiaUNIFESP, EPM, Depto. de Neurologia e NeurocirurgiaSciEL

Devenir du marquage sexuel du noyau dans les transplants hétéro-sexuels de muqueuse vésicale

SUMMARYTransplants of vesical mucous tissue in the dog to an animal of opposite sex keep their own sex karyotype.This seems to prove—to the contrary of what takes place for leucocytic sex corpuscles—that the sex karyotype of some cells does not undergo alterations under the action of hormone impregnation.</jats:p

Simple and non toxic enzyme immobilization onto platinum electrodes for detection of metabolic molecules in the rat brain using silicon micro-needles

AbstractWe report the successful fabrication of microelectrode biosensors, onto Pt wires or silicon micro-needles, based on oxidase immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE). We found that biosensors made with PEGDE exhibited high sensitivity and a response time on the order of seconds. Glucose biosensors implanted in the central nervous system of anesthetized rats reliably monitored changes in brain glucose levels induced by sequential administration of insulin and glucose. PEGDE provides a simple, low cost, non-toxic alternative for the preparation of in vivo microelectrode biosensors. This innovative method was applied to functionalize Pt microelectrodes deposited onto silicon microprobes. Their operating parameters were characterized and validated in vivo

Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation.

Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used as an additive for cross-linking polymers bearing amine, hydroxyl, or carboxyl groups. However, the idea of using PEGDE alone for immobilizing proteins on biosensors has never been thoroughly explored. We report the successful fabrication of microelectrode biosensors based on glucose oxidase, d-amino acid oxidase, and glutamate oxidase immobilized using PEGDE. We found that biosensors made with PEGDE exhibited high sensitivity and a response time on the order of seconds, which is sufficient for observing biological processes in vivo. The enzymatic activity on these biosensors was highly stable over several months when they were stored at 4 \ub0C, and over at least 3d at 37 \ub0C. Glucose microelectrode biosensors implanted in the central nervous system of anesthetized rats reliably monitored changes in brain glucose levels induced by sequential administration of insulin and glucose. PEGDE provides a simple, low cost, non-toxic alternative for the preparation of in vivo microelectrode biosensors