The Relationship Between the Employment of School Resource Officers, School Discipline, and School-Based Arrests Variables

The school resource officer (SRO) program is a program developed in the United States with the goal of making schools a safer environment for students across all grades (Cray & Weiler, 2011). To date, the majority of research surrounding SRO programs focuses on recommended characteristics and qualities of SROs, as well as appropriate utilization of SROs (Weiler & Cray, 2011). However, relatively little is known about the effect of increased presence of SRO’s in the school setting. With SRO’s being tasked with disciplinarian roles Barnes (2016), it would be important to look at the effect of SRO’s on school discipline variables such as out-of-school suspension (OSS). With OSS being linked to increased risk for arrest (Theriot, 2009), it would be important to analyze the effect of these variables on each other. Taking it one step further, minority populations are typically disciplined at a higher rate than their white peers (Okonofua & Eberhardt, 2015). The purpose of this study is to examine the increased presence of SRO’s, OSS and minority and their effect on school-based arrest. The results support previous research in finding that OSS and number of SRO’s employed were significant predictors of school-based arrest. However, percentage of minority population was not found to be a predictor of school-based arrest. More research is needed to understand the extent of the relationship between OSS, SRO’s, and school-based arrests and how it might be possible to reduce this connection

Preclinical translation of exosomes derived from mesenchymal stem/stromal cells.

Exosomes are nanovesicles secreted by virtually all cells. Exosomes mediate the horizontal transfer of various macromolecules previously believed to be cell-autonomous in nature, including nonsecretory proteins, various classes of RNA, metabolites, and lipid membrane-associated factors. Exosomes derived from mesenchymal stem/stromal cells (MSCs) appear to be particularly beneficial for enhancing recovery in various models of disease. To date, there have been more than 200 preclinical studies of exosome-based therapies in a number of different animal models. Despite a growing number of studies reporting the therapeutic properties of MSC-derived exosomes, their underlying mechanism of action, pharmacokinetics, and scalable manufacturing remain largely outstanding questions. Here, we review the global trends associated with preclinical development of MSC-derived exosome-based therapies, including immunogenicity, source of exosomes, isolation methods, biodistribution, and disease categories tested to date. Although the in vivo data assessing the therapeutic properties of MSC-exosomes published to date are promising, several outstanding questions remain to be answered that warrant further preclinical investigation

Immunoregulatory Potential of Exosomes Derived from Cancer Stem Cells.

Head and neck squamous cell carcinomas (HNSCCs) are malignancies that originate in the mucosal lining of the upper aerodigestive tract. Despite advances in therapeutic interventions, survival rates among HNSCC patients have remained static for years. Cancer stem cells (CSCs) are tumor-initiating cells that are highly resistant to treatment, and are hypothesized to contribute to a significant fraction of tumor recurrences. Consequently, further investigations of how CSCs mediate recurrence may provide insights into novel druggable targets. A key element of recurrence involves the tumor's ability to evade immunosurveillance. Recent published reports suggest that CSCs possess immunosuppressive properties, however, the underlying mechanism have yet to be fully elucidated. To date, most groups have focused on the role of CSC-derived secretory proteins, such as cytokines and growth factors. Here, we review the established immunoregulatory role of exosomes derived from mixed tumor cell populations, and propose further study of CSC-derived exosomes may be warranted. Such studies may yield novel insights into new druggable targets, or lay the foundation for future exosome-based diagnostics

One-carbon oxidative annulations of 1,3-enynes by catalytic C–H functionalization and 1,4-rhodium(III) migration

Rhodium(III)-catalyzed C-H functionalization-oxidative annulations of aromatic substrates with 1,3-enynes that contain allylic hydrogen atoms cis to the alkyne are described. The key step in these reactions is an alkenyl-to-allyl 1,4-rhodium(III) migration to give electrophilic pi-allylrhodium(III) species. Nucleophilic trapping of these species gives heterocycles such as benzopyrans, isobenzofuranones, and isoindolinones

Precipitation and Not Cover Crop Composition Influenced Corn Economic Optimal N Rate and Yield

The effects of single species cover crops on corn (Zea mays L.) N requirement and grain yield are well studied throughout the U.S. Midwest. However, comparing cover crop mixes that include different compositions of grass and broadleaf species is limited. Fourteen corn N response experiments were conducted in South Dakota from 2018 to 2021. Fall cover crops planted after small grain harvest were mixtures of dominantly grasses, broadleaves, a 50/50 grass/broadleaf mixture, and a no cover crop control. Compared to the control, including a cover crop led to no differences in economic optimal N rate (EONR) and yield at zero N (0N) and yield at EONR 44%, 62%, and 83% of the time, respectively. As spring cover crop/residue biomass and its C and N content increased, corn yield at EONR decreased and EONR increased when including cover crops (R2 = 0.36–0.56). Including cover crops reduced EONR and resulted in a similar yield when precipitation increased above 850 mm. When differences occurred with economic return from N, including a cover crop reduced economic return in 3 site-years (mean decrease of US$358 ha−1) and in only 1 site-year did including a grass cover crop increase economic return from N (+US$335 ha−1). Thus, in the first year of growing cover crops (i.e., grasses, broadleaves, or a grass/broadleaf mix) before corn, growers can normally expect some differences in EONR. However, with the appropriate rate of N, yield at EONR is maintained and any economic differences from N normally minimized

Cover Crops Did Not Improve Soil Health but Hydroclimatology May Guide Decisions Preventing Cash Crop Yield Loss

Introduction: Cover crop (CC) is an essential tool to improve or maintain soil health, potentially improving cash crop productivity. Several recent reports of cash crop yield reduction following cover cropping necessitated this research to guide efficient CC decisions in the season before corn (Zea mays) or soybean (Glycine max) is to be grown.Methods: Therefore, we designed this multi-year, multi-location study to include the farmers who plant CC following the harvest of a small grain crop, majorly wheat (Triticum aestivum) or oats (Avena sativa), and then grow corn or soybean cash crop in the subsequent season. We also selected the farmers who used a fall CC mix that was winter-terminated, to avoid further complexities. The major objective of this study was to document soil health changes and cash crop yields following CC in eight selected locations around SD for three consecutive CC seasons between 2017-2020. Experimental plots were laid out at the farmercooperators’ CC fields, where no cover (NC) ‘control’ was tested against CC in a randomized complete block design (RCBD). Soil samples were analyzed for selected soil health indicators (SHIs): potentially mineralizable nitrogen (PMN), permanganate oxidizable carbon (POXC), soil respiration (SR), soil microbial biomass (SMB), soil nitrate-nitrogen, soil organic matter (SOM), and other basic soil properties (pH, electrical conductivity, etc.); crop and residue biomass were calculated, and cash crop economic yields were measured.Results and discussion: No statistically significant (p30 g kg-1). These findings directed us to investigate hydroclimatological parameters and climatological indices such as accumulated precipitation, standardized precipitation index (SPI), and standardized precipitation-evapotranspiration index (SPEI) for their impact on CC’s influence on cash crop yields.Conclusion: Our analyses indicated that hydroclimatology, especially SPEI for the month before CC planting can be used as a tool to guide successful CC decisions, reducing the risk of cash crop yield loss. Further investigations with SPI and SPEI, along with other climatological parameters are needed to explore and design better CC management tools

Cover Crop Composition in Long-term No-till Soils in Semi-arid Environments Do Not Influence Soil Health Measurements after One Year

Evaluating the influence of grass or broadleaf cover crops on soil health measurements is common in the northern US Midwest. However, the comparison among different cover crop mixtures, including blends of both grass and broadleaf species is limited. In 2018–2020, cover crop experiments were conducted in South Dakota at 11 site-years. Cover crops were planted in the summer after small grains harvest as mixtures of dominantly grasses or broadleaves, a 50/50 grass/broadleaf mixture, and a no cover crop control. Soil and above-ground plant residue samples were collected in the fall before winter termination and in the spring before corn planting. Soil samples were analyzed for permanganate oxidizable carbon, potentially mineralizable nitrogen, and soil respiration. Fall and spring above-ground plant biomass in the cover crop plots were similar to the no cover crop control plots in seven of 11 site-years. Thus, growing cover crop mixes may accelerate decomposition of above-ground plant residue, possibly due to higher microbial diversity and activity under cover crops. However, including cover crops regardless of the mixture did not improve selected biological soil health indicators. Weather and soil properties (precipitation, soil organic matter, and pH) were related to differences in soil health measurements among site-years. Overall, in the first year of planting a multi-species mixture of grasses and/or broadleaves after small grain harvest, growers should not expect to find differences in soil health measurements. Long-term trials are needed to determine whether these different cover crop mixtures change soil health over time

Artificial escape from XCI by DNA methylation editing of the CDKL5 gene.

A significant number of X-linked genes escape from X chromosome inactivation and are associated with a distinct epigenetic signature. One epigenetic modification that strongly correlates with X-escape is reduced DNA methylation in promoter regions. Here, we created an artificial escape by editing DNA methylation on the promoter of CDKL5, a gene causative for an infantile epilepsy, from the silenced X-chromosomal allele in human neuronal-like cells. We identify that a fusion of the catalytic domain of TET1 to dCas9 targeted to the CDKL5 promoter using three guide RNAs causes significant reactivation of the inactive allele in combination with removal of methyl groups from CpG dinucleotides. Strikingly, we demonstrate that co-expression of TET1 and a VP64 transactivator have a synergistic effect on the reactivation of the inactive allele to levels >60% of the active allele. We further used a multi-omics assessment to determine potential off-targets on the transcriptome and methylome. We find that synergistic delivery of dCas9 effectors is highly selective for the target site. Our findings further elucidate a causal role for reduced DNA methylation associated with escape from X chromosome inactivation. Understanding the epigenetics associated with escape from X chromosome inactivation has potential for those suffering from X-linked disorders

Cover Crop Effect on Subsequent Wheat Yield in the Central Great Plains

Crop production systems in the water-limited environment of the semiarid central Great Plains may not have potential to profitably use cover crops because of lowered subsequent wheat (Triticum asestivum L.) yields following the cover crop. Mixtures have reportedly shown less yield-reducing effects on subsequent crops than single-species plantings. This study was conducted to determine winter wheat yields following both mixtures and single-species plantings of spring-planted cover crops. The study was conducted at Akron, CO, and Sidney, NE, during the 2012–2013 and 2013–2014 wheat growing seasons under both rainfed and irrigated conditions. Precipitation storage efficiency before wheat planting, wheat water use, biomass, and yield were measured and water use efficiency and harvest index were calculated for wheat following four single-species cover crops (flax [Linum usitatissimum L.], oat [Avena sativa L.], pea [Pisum sativum ssp. arvense L. Poir], rapeseed [Brassica napus L.]), a 10-species mixture, and a fallow treatment with proso millet (Panicum miliaceum L.) residue. There was an average 10% reduction in wheat yield following a cover crop compared with following fallow, regardless of whether the cover crop was grown in a mixture or in a single-species planting. Yield reductions were greater under drier conditions. The slope of the wheat water use–yield relationship was not significantly different for wheat following the mixture (11.80 kg ha–1 mm–1) than for wheat following single-species plantings (12.32–13.57 kg ha–1 mm–1). The greater expense associated with a cover crop mixture compared with a single species is not justified

Catalyst-Controlled Divergent C–H Functionalization of Unsymmetrical 2-Aryl Cyclic 1,3-Dicarbonyl Compounds with Alkynes and Alkenes

Achieving site-selective, switchable C–H functionalizations of substrates that contain several different types of reactive C–H bonds is an attractive objective to enable the generation of different products from the same starting materials. Herein, we demonstrate the divergent C–H functionalization of unsymmetrical 2-aryl cyclic 1,3-dicarbonyl compounds that contain two distinct, nonadjacent sites for initial C–H functionalization, where product selectivity is achieved through catalyst control. By use of a palladium–N-heterocyclic carbene complex as the precatalyst, these substrates undergo oxidative annulation with alkynes to provide spiroindenes exclusively. In contrast, a ruthenium-based catalyst system gives benzopyrans as the major products. Examples of divergent, oxidative C–H alkenylations of the same substrates are also provided