Mutation analysis of BRCA1 and BRCA2 genes in Iranian high risk breast cancer families

Background: Telomerase is a ribonucleoprotein enzyme that synthesises telomeres after cell division and maintains chromosomal stability leading to cellular immortalization. Telomerase has been associated with negative prognostic indicators in some studies. The present study aims to detect any association between telomerase sub-units: hTERT and hTR and the prognostic indicators including tumour's size and grade, nodal status and patient's age. Methods: Tumour samples from 46 patients with primary invasive breast cancer and 3 patients with benign tumours were collected. RT-PCR analysis was used for the detection of hTR, hTERT, and PGM1 (as a housekeeping) genes expression. Results: The expression of hTR and hTERT was found in 31(67.4%) and 38 (82.6%) samples respectively. We observed a significant association between hTR gene expression and younger age at diagnosis (p = 0.019) when comparing patients ≤ 40 years with those who are older than 40 years. None of the benign tumours expressed hTR gene. However, the expression of hTERT gene was revealed in 2 samples. No significant association between hTR and hTERT expression and tumour's grade, stage and nodal status was seen. Conclusion: The expression of hTR and hTERT seems to be independent of tumour's stage. hTR expression probably plays a greater role in mammary tumourogenesis in younger women (≤ 40 years) and this may have therapeutic implications in the context of hTR targeting strategies

The influence of parent austenite characteristics on the intervariant boundary network in a lath martensitic steel

The influence of the parent austenite deformation state on the intervariant boundary network (i.e., population, plane orientation, and connectivity) of a lath martensitic microstructure was investigated using conventional EBSD mapping and five-parameter boundary analysis approach along with quantification of boundary connectivity using homology metrics. The lath martensite largely revealed a bimodal misorientation angle distribution, closely matched with the Kurdjumov–Sachs (K–S) orientation relationship. The application of deformation significantly changed the distribution, gradually reducing the intensity of the 60° misorientation angle peak. This was largely ascribed to substructure development within the parent austenite upon deformation, which stimulates particular variant/s having a habit plane (011)α′ closely parallel to the primary (111)γ and/or secondary (11¯¯¯1)γ slip plane. The interaction of these variants eventually promoted specific intervariant boundaries (e.g., 60°/[111], 10.5°/[011], and 49.5°/[110]) at the expense of 60°/[011]. The application of deformation in the parent austenite did not change the intervariant boundary plane character distribution, which mostly exhibited an anisotropic character terminated on {110} planes because of the displacive nature of the martensitic transformation. However, the extent of anisotropy progressively decreased with increasing strain in the austenite prior to transformation. The grain boundary network connectivity was markedly altered due to the local variant selection induced by the deformation. Deformation in the austenite regime generally decreased the connectivity of boundaries having a {110} plane orientation. The intervariant boundaries with the {110} twist character also displayed a similar trend, though the connectivity of {110} tilt boundaries progressively enhanced with increasing strain. The former was closely matched with a decrease in the population of 60°/[110] intervariant boundaries with the strain. The current findings suggest that the intervariant boundary network of lath martensite can be manipulated through changes in the parent austenite deformation state which, in turn, can be used to enhance key final product properties such as toughness.publishedVersio

Razumikhin Stability Theorem for Fractional Systems with Delay

Fractional calculus techniques and methods started to be applied successfully during the last decades in several fields of science and engineering. In this paper we studied the stability of fractional-order nonlinear time-delay systems for Riemann-Liouville and Caputo derivatives and we extended Razumikhin theorem for the fractional nonlinear time-delay systems

Visualizing chemical states and defects induced magnetism of graphene oxide by spatially-resolved-X-ray microscopy and spectroscopy

[[abstract]]This investigation studies the various magnetic behaviors of graphene oxide (GO) and reduced graphene oxides (rGOs) and elucidates the relationship between the chemical states that involve defects therein and their magnetic behaviors in GO sheets. Magnetic hysteresis loop reveals that the GO is ferromagnetic whereas photo-thermal moderately reduced graphene oxide (M-rGO) and heavily reduced graphene oxide (H-rGO) gradually become paramagnetic behavior at room temperature. Scanning transmission X-ray microscopy and corresponding X-ray absorption near-edge structure spectroscopy were utilized to investigate thoroughly the variation of the C 2p(π*) states that are bound with oxygen-containing and hydroxyl groups, as well as the C 2p(σ*)-derived states in flat and wrinkle regions to clarify the relationship between the spatially-resolved chemical states and the magnetism of GO, M-rGO and H-rGO. The results of X-ray magnetic circular dichroism further support the finding that C 2p(σ*)-derived states are the main origin of the magnetism of GO. Based on experimental results and first-principles calculations, the variation in magnetic behavior from GO to M-rGO and to H-rGO is interpreted, and the origin of ferromagnetism is identified as the C 2p(σ*)- derived states that involve defects/vacancies rather than the C 2p(π*) states that are bound with oxygen-containing and hydroxyl groups on GO sheets.[[notice]]補正完

Time to first recurrence, pattern of recurrence, and survival after recurrence in endometrial cancer according to the molecular classification.

OBJECTIVE Despite its generally favorable prognosis at primary diagnosis, recurrence of endometrial cancer remains an important clinical challenge. The aim of this study was to analyze the value of molecular classification in recurrent endometrial cancer. METHODS This study included patients with recurrent endometrial cancer who underwent primary surgical treatment between 2004 and 2015 at the Karolinska University Hospital, Sweden and the Bern University Hospital, Switzerland (KImBer cohort) with molecular classification of the primary tumor. RESULTS Out of 594 molecularly classified endometrial cancer patients, 101 patients experienced recurrence, consisting of 2 POLEmut, 33 MMRd, 30 p53abn, and 36 NSMP tumors. Mean age at recurrence was 71 years and mean follow-up was 54 months. Overall, median time to first recurrence was 16 months (95% CI 12-20); with the shortest median time in MMRd patients, with 13 months (95% CI 5-21). The pattern of recurrence was distinct among molecular subgroups: MMRd tumors experienced more locoregional, while p53abn cases showed more abdominal recurrences (P = .042). Median survival after recurrence was best for MMRd cases (43 months, 95% CI 11-76), compared to 39 months (95% CI 21-57) and 10 months (95% CI 7-13) for the NSMP and p53abn cases respectively (log-rank, P = .001). CONCLUSION Molecular classification is a significant indicator of survival after recurrence in endometrial cancer patients, and patterns of recurrence differ by molecular subgroups. While MMRd endometrial cancer show more locoregional recurrence and the best survival rates after recurrence, p53abn patients experience abdominal recurrence more often and had the worst prognosis of all recurrent patients

Discerning primary versus diagenetic signals in carbonate carbon and oxygen isotope records: An example from the Permian-Triassic boundary of Iran

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordSedimentary successions across the Permian-Triassic boundary (PTB) are marked by a prominent negative carbon isotope excursion. This excursion, found in both fossil (e.g., brachiopod) and bulk carbonate at many sites around the world, is generally considered to be related to a global carbon cycle perturbation. Oxygen isotopes also show a negative excursion across the PTB, but because δ18O is more prone to diagenetic overprint (especially in bulk carbonate), these data are often not used in palaeoenvironmental analyses. In the present study, bulk-rock and brachiopod δ13C and δ18O, as well as conodont δ18O, were analyzed in PTB successions at Kuh-e-Ali Bashi and Zal (NW Iran) in order to evaluate diagenetic overprints on primary marine isotopic signals. The results show that the use of paired C-O isotopes and Mn-Sr concentrations is not sufficient to identify diagenetic alteration in bulk materials, because δ13C-δ18O covariation can be due to environmental factors rather than diagenesis, and Sr/Ca and Mn/Ca ratios can vary as a function of bulk-rock lithology. Comparison of δ13C profiles shows that all bulk carbonate is altered to some degree, although the general bulk-rock trend mimics that of the brachiopod data with a systematic offset of -1.2(±0.4)‰. This suggests that the first-order δ13C trend in bulk carbonate is generally robust but that the significance of small-scale carbon isotope fluctuations is uncertain, especially when such fluctuations are linked to lithologic variation. The PTB interval, which is marked by a low-carbonate 'Boundary Clay' in the study sections, may be especially prone to diagenetic alteration, e.g., via late-stage dolomitization. Comparison of oxygen-isotope profiles for bulk rock and well-preserved fossils (both brachiopods and conodonts) shows that the former are offset by -2.1(±0.4)‰. Diagenetic modeling suggests that these offsets were the product mainly of early diagenesis at burial temperatures of ~50-80°C and water/rock ratios of <10. Authigenic carbonates precipitated during early diagenesis represent a potentially major sink for isotopically light carbon at a global scale that has received relatively little attention to date.TJA thanks the Sedimentary Geology and Paleobiology program of the U.S. National Science Foundation (NSF EAR-1053449), the NASA Exobiology program (NNX13AJ1IG), and the China University of Geosciences—Wuhan (SKL-GPMR program GPMR201301, and SKL-BGEG programBGL21407) for their support. This study was funded by Deutsche Forschungsgemeinschaft (DFG; projects KO1829/12-1, KO1829/12-2 and KO2011/8-1)

High-resolution stratigraphy of the Changhsingian (Late Permian) successions of NW Iran and the Transcaucasus based on lithological features, conodonts and ammonoids

The Permian–Triassic boundary sections in north-western Iran belong to the most complete successions, in which the largest mass extinction event in the history of the Earth can be studied. We investigated the Changhsingian stage in six sections in the area of Julfa (Aras Valley) for their lithology, conodonts and ammonoids. Revision of the biostratigraphy led to the separation of 10 conodont zones (from bottom to top Clarkina orientalis–C. subcarinata interval zone, C. subcarinata, C. changxingensis, C. bachmanni, C. nodosa, C. yini, C. abadehensis, C. hauschkei, Hindeodus praeparvus–H. changxingensis and Merrilina ultima–Stepanovites ?mostleri zones) and 8 ammonoid zones (from bottom to top Iranites transcaucasius–Phisonites triangulus, Dzhulfites nodosus, Shevyrevites shevyrevi, Paratirolites trapezoidalis, P. waageni, Stoyanowites dieneri, Abichites stoyanowi and Arasella minuta zones). The new ammonoid genera Stoyanowites and Arasella are described

Optimal neural network feature selection for spatial-temporal forecasting

In this paper, we show empirical evidence on how to construct the optimal feature selection or input representation used by the input layer of a feedforward neural network for the propose of forecasting spatial-temporal signals. The approach is based on results from dynamical systems theory, namely the non-linear embedding theorems. We demonstrate it for a variety of spatial-temporal signals, with one spatial and one temporal dimensions, and show that the optimal input layer representation consists of a grid, with spatial/temporal lags determined by the minimum of the mutual information of the spatial/temporal signals and the number of points taken in space/time decided by the embedding dimension of the signal. We present evidence of this proposal by running a Monte Carlo simulation of several combinations of input layer feature designs and show that the one predicted by the non-linear embedding theorems seems to be optimal or close of optimal. In total we show evidence in four unrelated systems: a series of coupled Henon maps; a series of couple Ordinary Differential Equations (Lorenz-96) phenomenologically modelling atmospheric dynamics; the Kuramoto-Sivashinsky equation, a partial differential equation used in studies of instabilities in laminar flame fronts and finally real physical data from sunspot areas in the Sun (in latitude and time) from 1874 to 2015.Comment: 11 page

Zealots Attack and the Revenge of the Commons: Quality vs Quantity in the Best-of-n

© 2020, Springer Nature Switzerland AG. In this paper we study the effect of inflexible individuals with fixed opinions, or zealots, on the dynamics of the best-of-n collective decision making problem, using both the voter model and the majority rule decision mechanisms. We consider two options with different qualities, where the lower quality option is associated to a higher number of zealots. The aim is to study the trade-off between option quality and zealot quantity for two different scenarios: one in which all agents can modulate dissemination of their current opinion proportionally to the option quality, and one in which this capability is only possessed by the zealots. In both scenarios, our goal is to determine in which conditions consensus is more biased towards the high or low quality option, and to determine the indifference curve separating these two regimes. Using both numerical simulations and ordinary differential equation models, we find that: i) if all agents can modulate the dissemination time based on the option quality, then consensus can be driven to the high quality option when the number of zealots for the other option is not too high; ii) if only zealots can modulate the dissemination time based on the option quality, whil e all normal agents cannot distinguish the two options and cannot differentially disseminate, then consensus no longer depends on the quality and is driven to the low quality option by the zealots

Structural and optical properties of high quality zinc-blende/wurtzite GaAs hetero-nanowires

The structural and optical properties of 3 different kinds of GaAs nanowires with 100% zinc-blende structure and with an average of 30% and 70% wurtzite are presented. A variety of shorter and longer segments of zinc-blende or wurtzite crystal phases are observed by transmission electron microscopy in the nanowires. Sharp photoluminescence lines are observed with emission energies tuned from 1.515 eV down to 1.43 eV when the percentage of wurtzite is increased. The downward shift of the emission peaks can be understood by carrier confinement at the interfaces, in quantum wells and in random short period superlattices existent in these nanowires, assuming a staggered band-offset between wurtzite and zinc-blende GaAs. The latter is confirmed also by time resolved measurements. The extremely local nature of these optical transitions is evidenced also by cathodoluminescence measurements. Raman spectroscopy on single wires shows different strain conditions, depending on the wurtzite content which affects also the band alignments. Finally, the occurrence of the two crystallographic phases is discussed in thermodynamic terms.Comment: 24 page