8 research outputs found
Molecular analysis of variability among genotypes of <i style="mso-bidi-font-style:normal">Abrus precatorius </i><span style="mso-bidi-font-style:italic">L. with different seed coat colours using RAPD and ISSR markers </span>
273-276Three genotypes
of a medicinal, climbing herb Abrus precatorius L. (Family: Fabaceae), having different seed coat colour (red,
black and white), were subjected to
molecular analysis using PCR based RAPD and ISSR markers. Twenty RAPD
(decamers) and fourteen ISSR primers were screened for their amplification
potential. Of which 12 RAPD and 5 ISSR primers produced clear and reproducible
amplified products. These primers yielded a total of 149 amplified fragments
with an average of 8.76 bands per primer including 14 (9.39%) polymorphic
fragments. In the study, ISSR fingerprinting (14.28%) detected more polymorphic
loci as compare to RAPDs (7.89%). The data analyses based on Jaccard’s
similarity coefficient and UPGMA cluster analysis revealed that genotypes with
black and white seed coats were more diverse as compared to genotype with red
seed coat. Combined data of RAPD and ISSR further revealed that Abrus with white seed coat was more
closely related to those having red seed coat.
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