A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Over-representation of specific regions of chromosome 22 in cells from human glioma correlate with resistance to 1,3-bis(2-chloroethyl)-1-nitrosourea

BACKGROUND: Glioblastoma multiforme is the most malignant form of brain tumor. Despite treatment including surgical resection, adjuvant chemotherapy, and radiation, these tumors typically recur. The recurrent tumor is often resistant to further therapy with the same agent, suggesting that the surviving cells that repopulate the tumor mass have an intrinsic genetic advantage. We previously demonstrated that cells selected for resistance to 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) are near-diploid, with over-representation of part or all of chromosomes 7 and 22. While cells from untreated gliomas often have over-representation of chromosome 7, chromosome 22 is typically under-represented. METHODS: We have analyzed cells from primary and recurrent tumors from the same patient before and after in vitro selection for resistance to clinically relevant doses of BCNU. Karyotypic analyses were done to demonstrate the genetic makeup of these cells, and fluorescent in situ hybridization analyses have defined the region(s) of chromosome 22 retained in these BCNU-resistant cells. RESULTS: Karyotypic analyses demonstrated that cells selected for BCNU resistance were near-diploid with over-representation of chromosomes 7 and 22. In cells where whole copies of chromosome 22 were not identified, numerous fragments of this chromosome were retained and inserted into several marker and derivative chromosomes. Fluorescent in situ hybridization analyses using whole chromosome paints confirmed this finding. Additional FISH analysis using bacterial artificial chromosome probes spanning the length of chromosome 22 have allowed us to map the over-represented region to 22q12.3–13.32. CONCLUSION: Cells selected for BCNU resistance either in vivo or in vitro retain sequences mapped to chromosome 22. The specific over-representation of sequences mapped to 22q12.3–13.32 suggest the presence of a DNA sequence important to BCNU survival and/or resistance located in this region of chromosome 22

Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR

Here we present Cys-Ph-TAHA, a new nonadentate lanthanide tag for the paramagnetic labelling of proteins. The tag can be easily synthesized and is stereochemically homogenous over a wide range of temperatures, yielding NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large residual dipolar couplings and pseudocontact shifts that could be measured easily and agreed very well with the protein structure. We show that Cys-Ph-TAHA can be used to label large proteins that are biochemically challenging such as the Lac repressor in a 90 kDa ternary complex with DNA and inducer

Apraxia for differentiating Alzheimer’s disease from subcortical vascular dementia and mild cognitive impairment

Serhat Ozkan,1 Demet Ozbabalik Adapinar,1 Nese Tuncer Elmaci,2 Didem Arslantas31Department of Neurology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey; 2Department of Neurology, Marmara University Medical Faculty, Istanbul, Turkey; 3Department of Public Health, Eskisehir Osmangazi University Medical Faculty, Eskisehir, TurkeyAbstract: Although ideomotor limb apraxia is considered to be a typical sign of cortical pathologies such as Alzheimer’s disease (AD), it has been also reported in subcortical neurodegenerative diseases and vascular lesions. We aimed to investigate the difference between AD, subcortical vascular dementia (SVaD) and mild cognitive impairment (MCI) patients by means of ideomotor limb apraxia frequency and severity. Ninety-six AD, 72 SVaD, and 84 MCI patients were assessed with the mini-mental status examination (MMSE), clinical dementia rating (CDR) and the apraxia screening test of TULIA (AST). Apraxia was significantly more frequent in the AD patients (32.3%) than in both of the SVaD (16.7%) and MCI (4.8%) patients. The frequency of apraxia was also significantly higher in SVaD patients than in MCI patients. AD patients had significantly lower apraxia scores than both SVaD and MCI patients. In addition, a significant difference was found between SVaD and MCI patients in terms of apraxia scores. These results suggest that the widespread belief of the association between apraxia and cortical dementias is not exactly correct. The significant difference between both of the dementia groups and the MCI patients suggests that the absence of apraxia can be an indicator for MCI diagnosis.Keywords: apraxia, Alzheimer’s disease, subcortical vascular dementia, mild cognitive impairmen

The Level of Cyber-Ostracism and Self-Esteem in Medical Faculty Students

Abstract Background Social media, which has become an indispensable part of our lives in recent years, is important for young people to socialize in online groups and to develop their self-esteem (SE). One of the dangers of increased use of social media is cyber ostracism (CO), defined as not being able to join a group and being ignored on social media. In the study, it was aimed to evaluate the level of CO and SE in medical faculty students. Methods This is a cross-sectional study conducted on students of Eskişehir Osmangazi University Faculty of Medicine in 2021 spring semester. The questionnaire prepared based on the literature was filled out online by the participants. The study group consisted of 1092 (70% response rate) students who agreed to participate in the survey. The CO Scale was used to assess the level of CO, and the Rosenberg SE Scale was used for the assessment of SE. Mann Whitney-U test, Kruskal Wallis test, Spearman correlation analysis and Multiple Linear Regression analysis were used to analyze the data. Results In the study group; 593 (54.3%) were female. Their ages ranged from 17-27, with a mean of 21.6 ± 2.0 years. The scores obtained from the CO scale ranged from 14 to 70, with an average of 24.8 ± 9.9 points. A moderate positive correlation was found between the scores the students got from the CO Scale and the SE Scale (r: 0.49, p: 0.001). Multiple Linear Regression analysis showed that; living in an extended family, not sparing time for hobbies during the day, having a type-A personality, poor face-to-face communication with peers, negative evaluation of parental attitudes, age at first use of smartphone below years 12 or less, use of smartphones for 5 hours or more a day, and Creating membership in social media by hiding their identity was a predictor for CO (F: 30.338, R2: 0.212, p &amp;lt; 0.001). Conclusions It can be said that CO is at a moderate level in medical faculty students. An inverse relationship between CO and SE was found in the study. Key messages İt’s recommended to plan more comprehensive studies on CO which is a new concept for digital health. Public health policies to reduce the level of CO may contribute to higher SE in future physicians. </jats:sec