Physicochemical analysis of rotavirus segment 11 supports a 'modified panhandle' structure and not the predicted alternative tRNA-like structure (TRLS)

.Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements. Covariation studies and phylogenetic analysis exploring the potential structure of RNA11 of rotaviruses suggested that, besides the previously predicted "modified panhandle" structure, the 5' and 3' termini of one of the isoforms of the bovine rotavirus UKtc strain may interact to form a tRNA-like structure (TRLS). Such TRLSs have been identified in RNAs of plant viruses, where they are important for enhancing replication and packaging. However, using tRNA mimicry assays (in vitro aminoacylation and 3'- adenylation), we found no biochemical evidence for tRNA-like functions of RNA11. Capping, synthetic 3' adenylation and manipulation of divalent cation concentrations did not change this finding. NMR studies on a 5'- and 3'-deletion construct of RNA11 containing the putative intra-strand complementary sequences supported a predominant panhandle structure and did not conform to a cloverleaf fold despite the strong evidence for a predicted structure in this conserved region of the viral RNA. Additional viral or cellular factors may be needed to stabilise it into a form with tRNA-like properties

Tropical cyclone projections: changing climate threats for Pacific island defense installations

Potential changing climate threats in the tropical and subtropical North Pacific Ocean were assessed, using coupled ocean-atmosphere and atmosphere-only general circulation models, to explore their response to projected increasing greenhouse gas emissions. Tropical cyclone occurrence, described by frequency and intensity, near islands housing major U.S. defense installations was the primary focus. Four island regions-Guam and Kwajalein Atoll in the tropical northwestern Pacific, Okinawa in the subtropical northwestern Pacific, and Oahu in the tropical north-central Pacific-were considered, as they provide unique climate and geographical characteristics that either enhance or reduce the tropical cyclone risk. Guam experiences the most frequent and severe tropical cyclones, which often originate as weak systems close to the equator near Kwajalein and sometimes track far enough north to affect Okinawa, whereas intense storms are the least frequent around Oahu. From assessments of models that simulate well the tropical Pacific climate, it was determined that, with a projected warming climate, the number of tropical cyclones is likely to decrease for Guam and Kwajalein but remain about the same near Okinawa and Oahu; however, the maximum intensity of the strongest storms may increase in most regions. The likelihood of fewer but stronger storms will necessitate new localized assessments of the risk and vulnerabilities to tropical cyclones in the North Pacific

Needle(s) in the Haystack – Synchronous Multifocal Tumor Induced Osteomalacia

This is the author accepted manuscript. The final version is available from Endocrine Society via http://dx.doi.org/10.1210/jc.2015-3854MG is supported by the NIHR Cambridge Biomedical Research Centre

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

High-performance flexible perovskite solar cells exploiting Zn2SnO4 prepared in solution below 100 degrees C

Fabricating inorganic-organic hybrid perovskite solar cells (PSCs) on plastic substrates broadens their scope for implementation in real systems by imparting portability, conformability and allowing high-throughput production, which is necessary for lowering costs. Here we report a new route to prepare highly dispersed Zn2SnO4 (ZSO) nanoparticles at low-temperature (<100 degrees C) for the development of high-performance flexible PSCs. The introduction of the ZSO film significantly improves transmittance of flexible polyethylene naphthalate/indium-doped tin oxide (PEN/ITO)-coated substrate from similar to 75 to similar to 90% over the entire range of wavelengths. The best performing flexible PSC, based on the ZSO and CH3NH3PbI3 layer, exhibits steady-state power conversion efficiency (PCE) of 14.85% under AM 1.5G 100 mW . cm(-2) illumination. This renders ZSO a promising candidate as electron-conducting electrode for the highly efficient flexible PSC applications.ope

Breathing Current Domains in Globally Coupled Electrochemical Systems: A Comparison with a Semiconductor Model

Spatio-temporal bifurcations and complex dynamics in globally coupled intrinsically bistable electrochemical systems with an S-shaped current-voltage characteristic under galvanostatic control are studied theoretically on a one-dimensional domain. The results are compared with the dynamics and the bifurcation scenarios occurring in a closely related model which describes pattern formation in semiconductors. Under galvanostatic control both systems are unstable with respect to the formation of stationary large amplitude current domains. The current domains as well as the homogeneous steady state exhibit oscillatory instabilities for slow dynamics of the potential drop across the double layer, or across the semiconductor device, respectively. The interplay of the different instabilities leads to complex spatio-temporal behavior. We find breathing current domains and chaotic spatio-temporal dynamics in the electrochemical system. Comparing these findings with the results obtained earlier for the semiconductor system, we outline bifurcation scenarios leading to complex dynamics in globally coupled bistable systems with subcritical spatial bifurcations.Comment: 13 pages, 11 figures, 70 references, RevTex4 accepted by PRE http://pre.aps.or

Principal component analysis (PCA) and genetic diversity studies for discrimination of genotypes and yield parameters in ICRISAT Pearl Millet [Pennisetum glaucum (L.) R. Br.] accessions

PCA and cluster analysis were used to assess 50 pearl millet genotypes for 26 attributes to identify associations among individuals as well as their traits and patterns of variation. Among 26 PCs (Principal Components), eight unveiled eigenvalues greater than one, which accounted for 78.29 % of the total variability among the traits. The first component (PC1) had a great maximum variability of about 18.53 % with the highest eigenvalue. It was observed that PC1 revealed maximum variation in comparison with other 25 PCs, whereas PC2 to PC8 showed gradual reduction in variability with values 17.38, 11.71, 8.17, 7.37, 5.63, 5.0 and 4.49 percent respectively. Therefore, selecting lines and traits based on PC1 would be beneficial. The factor loading of PCs exhibited that PC1 accounted for maximum variability for traits like NTrPP (number of tillers per plant), LAI (leaf area index) and GY (grain yield) per plant. PC2 counted for 17.38 % of the total variance and exhibited the greatest variability for number of leaves per plant (NLPP). PC3 showed 11.71 % variability and taken maximum variability for leaf:stem ratio (LSR). PC4 exhibited 8.17 % of variability and possessed maximum variability for plant height (PtH), leaf width (LfW), flag leaf width (FgLW) and stem girth (SG) which pointed out the huge impact in the total variation of the genotypes. Cluster analysis recorded cluster III had desirable mean values for most of the traits studied. The genotype, viz. IP 8327 from cluster III would be used as parent for pearl millet improvement program

The importance of interacting climate modes on Australia’s contribution to global carbon cycle extremes

The global carbon cycle is highly sensitive to climate-driven fluctuations of precipitation, especially in the Southern Hemisphere. This was clearly manifested by a 20% increase of the global terrestrial C sink in 2011 during the strongest sustained La Niña since 1917. However, inconsistencies exist between El Niño/La Niña (ENSO) cycles and precipitation in the historical record; for example, significant ENSO-precipitation correlations were present in only 31% of the last 100 years, and often absent in wet years. To resolve these inconsistencies, we used an advanced temporal scaling method for identifying interactions amongst three key climate modes (El Niño, the Indian Ocean dipole, and the southern annular mode). When these climate modes synchronised (1999-2012), drought and extreme precipitation were observed across Australia. The interaction amongst these climate modes, more than the effect of any single mode, was associated with large fluctuations in precipitation and productivity. The long-term exposure of vegetation to this arid environment has favoured a resilient flora capable of large fluctuations in photosynthetic productivity and explains why Australia was a major contributor not only to the 2011 global C sink anomaly but also to global reductions in photosynthetic C uptake during the previous decade of drought

Introductory programming: a systematic literature review

As computing becomes a mainstream discipline embedded in the school curriculum and acts as an enabler for an increasing range of academic disciplines in higher education, the literature on introductory programming is growing. Although there have been several reviews that focus on specific aspects of introductory programming, there has been no broad overview of the literature exploring recent trends across the breadth of introductory programming. This paper is the report of an ITiCSE working group that conducted a systematic review in order to gain an overview of the introductory programming literature. Partitioning the literature into papers addressing the student, teaching, the curriculum, and assessment, we explore trends, highlight advances in knowledge over the past 15 years, and indicate possible directions for future research