An evaluation of the reliability of the pictorial scale of perceived water competence and its relationship with actual water competence

In its recent development, the Pictorial Scale of Perceived Water Competence (PSPWC) showed good face and construct validity. However, additional reliability and validity research is needed, including test-retest reliability and a demonstration of the relationship between PSPWEC test scores and actual water competence. Toward that aim, we administered the PSPWC to 124 children, aged 5–8 years. We repeated this test administration after one week for a subset of 55 children to determine its test-retest reliability, and the remaining 69 children also performed the fully aligned Actual Aquatic Skills Test (AAST) in an indoor swimming pool to provide data for our investigation of the relationship between PSPWC scores and actual water competence. We found good test-retest reliability, both at the global level (ICC = 0.81, n = 55) and at the level of individual skills (Weighted kappa coefficients from 0.58 to 0.90), with no significant differences between these two test scores. We also found a moderate positive relationship between PSPWC and AAST total scores (r = .64, n = 69), with no significant difference between total scores of actual and perceived water competencies. Children overestimated their competence in three specific skills: the back star, swimming on the front, and diving in deep water. While these results underline specific situations in which children’s higher self-perceptions of their water competence are a risk factor for their water safety, these data confirm that the PSPWC is reliable for measuring children’s perceived competencies in aquatic education and drowning prevention, and there is further support for its validity through a moderate correlation with actual water competencies

Pictorial scale of perceived water competence (PSPWC) testing manual

The Early Years Special Interest Group have completed a technical publication, ‘Pictorial Scale of Perceived Water Competence (PSPWC) Testing Manual’. This publication is the first to specifically focus and develop an assessment tool appropriate for young children aged 4 – 8 years old. It aims to address the following specific needs 1) to be accessible to this young age range, 2) to be suitable for children of different swimming levels, 3) cover all the aquatic fundamentals – water entry, breath control, buoyancy, balance, propulsion, immersion, water exit, gliding and vision. The idea to develop the PSPWC started during the Early Years Special Interest Group meeting at Laramie 2016 AIESEP, which then developed into a larger group of experts in order to develop and present the assessment tool. A four year process of critical analysis, preliminary face-validity, face-validity and content validity were completed in the development of each version of the PSPWC until the final testing manual was completed. Within the testing manual all aquatic skills and test items are represented through visual methodologies of drawings by a professional illustrator. In order to engage young children’s interests and to keep their attention to facilitate their understanding and to obtain more meaningful responses. The testing manual includes 3 level progressions for each situation, skill or test item, level 1 = “not able to do the skill”; level 2 = “skill in progress” and level 3 = “able to do the skill. The 3 level progression was developed as the expert group considered it more appropriate to have a process orientated scale showing a child’s developmental progression. The PSPWC can be applied for use in children (measurement of their own perceived water competence), in parents (measurements of their perception of children’s water competence) and/or in teachers (measurement of their perception of pupils’ / students’ water competence. The testing manual is accessible to all through open access publication

Differences between young children's actual, self-perceived and parent-perceived aquatic skills

As drowning is a leading cause of unintentional injury/death in children worldwide, perceptions of their actual aquatic skills are of critical importance. Children’s self perceptions may influence the risks they take, and parental perceptions may influence the degree of supervision deemed to be necessary for children in and around water. Accordingly, we examined the differences between young children’s actual, self-perceived and parent-perceived aquatic skills. Using a three-way repeated measures ANCOVA, we analyzed data from 134 child-parent dyads (56.0% boys; M age ¼ 7.1, SD ¼ 1.1 years; and 71.6% mothers). We measured self and parental perceptions of the child’s aquatic skills with the ‘Pictorial Scale of Perceived Water Competence’ (PSPWC), and we applied the exact same 17 test items of the PSPWC to assess the child’s actual aquatic skill level in the water. Controlling for years of swimming school experience, within-subject differences between the total scores on the ‘Actual Aquatic Skills Test’ (AAST) and both the child- and parentcompleted PSPWC indicated lower than actual estimates of the children’s aquatic skill level. The degree of disagreement against the AAST was more pronounced in parents than in 6-7 year-old children but was similar between parents and 8-9 yearold children, with these patterns being evident regardless of the children’s sex. Our study contributes to an ongoing validation of the PSPWC and represents a key advance in assessing and comparing children’s actual and perceived aquatic skill competence, using perfectly aligned instruments. Future research and practice might explore children’s actual aquatic skills in different contexts (e.g., open water), include perspectives of non-parent caregivers and assess perceived and actual water competence across development

Biogeographical distribution and diversity of microbes in methane hydrate-bearing deep marine sediments, on the Pacific Ocean Margin

The deep subseafloor biosphere is among the least-understood habitats on Earth, even though the huge microbial biomass therein plays an important role for potential long-term controls on global biogeochemical cycles. We report here the vertical and geographical distribution of microbes and their phylogenetic diversities in deeply buried marine sediments of the Pacific Ocean Margins. During the Ocean Drilling Program Legs 201 and 204, we obtained sediment cores from the Peru and Cascadia Margins that varied with respect to the presence of dissolved methane and methane hydrate. To examine differences in prokaryotic distribution patterns in sediments with or without methane hydrates, we studied >2,800 clones possessing partial sequences (400–500 bp) of the 16S rRNA gene and 348 representative clone sequences (≈1 kbp) from the two geographically separated subseafloor environments. Archaea of the uncultivated Deep-Sea Archaeal Group were consistently the dominant phylotype in sediments associated with methane hydrate. Sediment cores lacking methane hydrates displayed few or no Deep-Sea Archaeal Group phylotypes. Bacterial communities in the methane hydrate-bearing sediments were dominated by members of the JS1 group, Planctomycetes, and Chloroflexi. Results from cluster and principal component analyses, which include previously reported data from the West and East Pacific Margins, suggest that, for these locations in the Pacific Ocean, prokaryotic communities from methane hydrate-bearing sediment cores are distinct from those in hydrate-free cores. The recognition of which microbial groups prevail under distinctive subseafloor environments is a significant step toward determining the role these communities play in Earth’s essential biogeochemical processes

Atribacteria reproducing over millions of years in the Atlantic abyssal subseafloor

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Vuillemin, A., Vargas, S., Coskun, O. K., Pockalny, R., Murray, R. W., Smith, D. C., D'Hondt, S., & Orsi, W. D. Atribacteria reproducing over millions of years in the Atlantic abyssal subseafloor. Mbio, 11(5), (2020): e01937-20, doi:10.1128/mBio.01937-20.How microbial metabolism is translated into cellular reproduction under energy-limited settings below the seafloor over long timescales is poorly understood. Here, we show that microbial abundance increases an order of magnitude over a 5 million-year-long sequence in anoxic subseafloor clay of the abyssal North Atlantic Ocean. This increase in biomass correlated with an increased number of transcribed protein-encoding genes that included those involved in cytokinesis, demonstrating that active microbial reproduction outpaces cell death in these ancient sediments. Metagenomes, metatranscriptomes, and 16S rRNA gene sequencing all show that the actively reproducing community was dominated by the candidate phylum “Candidatus Atribacteria,” which exhibited patterns of gene expression consistent with fermentative, and potentially acetogenic, metabolism. “Ca. Atribacteria” dominated throughout the 8 million-year-old cored sequence, despite the detection limit for gene expression being reached in 5 million-year-old sediments. The subseafloor reproducing “Ca. Atribacteria” also expressed genes encoding a bacterial microcompartment that has potential to assist in secondary fermentation by recycling aldehydes and, thereby, harness additional power to reduce ferredoxin and NAD+. Expression of genes encoding the Rnf complex for generation of chemiosmotic ATP synthesis were also detected from the subseafloor “Ca. Atribacteria,” as well as the Wood-Ljungdahl pathway that could potentially have an anabolic or catabolic function. The correlation of this metabolism with cytokinesis gene expression and a net increase in biomass over the million-year-old sampled interval indicates that the “Ca. Atribacteria” can perform the necessary catabolic and anabolic functions necessary for cellular reproduction, even under energy limitation in millions-of-years-old anoxic sediments.This work was supported primarily by the Deutsche Forschungsgemeinschaft (DFG) project OR 417/1-1 granted to W.D.O. Preliminary work was supported by the Center for Dark Energy Biosphere Investigations project OCE-0939564 also granted to W.D.O. The expedition was funded by the US National Science Foundation through grant NSF-OCE-1433150 to S.D. and R.P. R.W.M. led the expedition. Shipboard microbiology efforts were supported by the Center for Dark Energy Biosphere Investigations (C-DEBI grant NSF-OCE-0939564). This is C-DEBI publication 545. This is a contribution of the Deep Carbon Observatory (DCO)

The Expectation Monad in Quantum Foundations

The expectation monad is introduced abstractly via two composable adjunctions, but concretely captures measures. It turns out to sit in between known monads: on the one hand the distribution and ultrafilter monad, and on the other hand the continuation monad. This expectation monad is used in two probabilistic analogues of fundamental results of Manes and Gelfand for the ultrafilter monad: algebras of the expectation monad are convex compact Hausdorff spaces, and are dually equivalent to so-called Banach effect algebras. These structures capture states and effects in quantum foundations, and also the duality between them. Moreover, the approach leads to a new re-formulation of Gleason's theorem, expressing that effects on a Hilbert space are free effect modules on projections, obtained via tensoring with the unit interval.Comment: In Proceedings QPL 2011, arXiv:1210.029

Bacterial diversity and community composition from seasurface to subseafloor

© The International Society for Microbial Ecology, 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in ISME Journal 10 (2016): 979–989, doi:10.1038/ismej.2015.175.We investigated compositional relationships between bacterial communities in the water column and those in deep-sea sediment at three environmentally distinct Pacific sites (two in the Equatorial Pacific and one in the North Pacific Gyre). Through pyrosequencing of the v4–v6 hypervariable regions of the 16S ribosomal RNA gene, we characterized 450 104 pyrotags representing 29 814 operational taxonomic units (OTUs, 97% similarity). Hierarchical clustering and non-metric multidimensional scaling partition the samples into four broad groups, regardless of geographic location: a photic-zone community, a subphotic community, a shallow sedimentary community and a subseafloor sedimentary community (greater than or equal to1.5 meters below seafloor). Abundance-weighted community compositions of water-column samples exhibit a similar trend with depth at all sites, with successive epipelagic, mesopelagic, bathypelagic and abyssopelagic communities. Taxonomic richness is generally highest in the water-column O2 minimum zone and lowest in the subseafloor sediment. OTUs represented by abundant tags in the subseafloor sediment are often present but represented by few tags in the water column, and represented by moderately abundant tags in the shallow sediment. In contrast, OTUs represented by abundant tags in the water are generally absent from the subseafloor sediment. These results are consistent with (i) dispersal of marine sedimentary bacteria via the ocean, and (ii) selection of the subseafloor sedimentary community from within the community present in shallow sediment.This study was funded by the Biological Oceanography Program of the US National Science Foundation (grant OCE-0752336) and by the NSF-funded Center for Dark Energy Biosphere Investigations (grant NSF-OCE-0939564)