192 research outputs found
Nicotinamide inhibits melanoma in vitro and in vivo
Background: Even though new therapies are available against melanoma, novel approaches are needed to overcome resistance and high-toxicity issues. In the present study the anti-melanoma activity of Nicotinamide (NAM), the amide form of Niacin, was assessed in vitro and in vivo. Methods: Human (A375, SK-MEL-28) and mouse (B16-F10) melanoma cell lines were used for in vitro investigations. Viability, cell-death, cell-cycle distribution, apoptosis, Nicotinamide Adenine Dinucleotide+ (NAD+), Adenosine Triphosphate (ATP), and Reactive Oxygen Species (ROS) levels were measured after NAM treatment. NAM anti-SIRT2 activity was tested in vitro; SIRT2 expression level was investigated by in silico transcriptomic analyses. Melanoma growth in vivo was measured in thirty-five C57BL/6 mice injected subcutaneously with B16-F10 melanoma cells and treated intraperitoneally with NAM. Interferon (IFN)-γ-secreting murine cells were counted with ELISPOT assay. Cytokine/chemokine plasmatic levels were measured by xMAP technology. Niacin receptors expression in human melanoma samples was also investigated by in silico transcriptomic analyses. Results: NAM reduced up to 90% melanoma cell number and induced: I) accumulation in G1-phase (40% increase), ii) reduction in S- A nd G2-phase (about 50% decrease), iii) a 10-fold increase of cell-death and 2.5-fold increase of apoptosis in sub-G1 phase, iv) a significant increase of NAD+, ATP, and ROS levels, v) a strong inhibition of SIRT2 activity in vitro. NAM significantly delayed tumor growth in vivo (p ≤ 0.0005) and improved survival of melanoma-bearing mice (p ≤ 0.0001). About 3-fold increase (p ≤ 0.05) of Interferon-gamma (IFN-γ) producing cells was observed in NAM treated mice. The plasmatic expression levels of 6 cytokines (namely: Interleukin 5 (IL-5), Eotaxin, Interleukin 12 (p40) (IL12(p40)), Interleukin 3 (IL-3), Interleukin 10 (IL-10) and Regulated on Activation Normal T Expressed and Secreted (RANTES) were significantly changed in the blood of NAM treated mice, suggesting a key role of the immune response. The observed inhibitory effect of NAM on SIRT2 enzymatic activity confirmed previous evidence; we show here that SIRT2 expression is significantly increased in melanoma and inversely related to melanoma-patients survival. Finally, we show for the first time that the expression levels of Niacin receptors HCAR2 and HCAR3 is almost abolished in human melanoma samples. Conclusion: NAM shows a relevant anti-melanoma activity in vitro and in vivo and is a suitable candidate for further clinical investigations
Dental pulp stem cells bioadhesivity: evaluation on mineral-trioxide-aggregate.
Stem cells are undifferentiated cells that have the capacity to self-renew. They have been discovered in many adult tissues, including teeth. Dental Pulp Mesenchymal Stem Cells (DP-MSCs) are involved in dental repair by activation of growth factors, released after caries and have the ability to regenerate a dentin-pulp-like complex. The molecular/cellular research gives the possibility to grow new tissues and biological structures for clinical applications, providing cells for therapies including cell transplantation and tissue engineering. In this study DP-MSCs were derived from dental pulp of 10 donors. To evaluate material toxicity, after in vitro isolation, the cells were seeded on mineral trioxide aggregate (MTA). Initial light microscopy investigation of cells revealed no signs of cell death due to toxicity or infection, on the contrary the scaffolds supplied an excellent support for cell structures, the cells proliferated and adhered to substrate. Similar observation was seen in scanning electron microscopy, in particular the cells had proliferated and spread, covering a considerable part of the surface of the biomaterials investigated, with an elaborate form of attachment, in fact, the cells formed a continuous layer on the upper surface of the MTA. In conclusion, the aim of this study is to demonstrate that DP-MSCs combined with MTA could be a potential source for regenerative medicine, encouraging further study to evaluate the new-dentin formation
The linearity response of the Planck-LFI flight model receivers
In this paper we discuss the linearity response of the Planck-LFI receivers,
with particular reference to signal compression measured on the 30 and 44 GHz
channels. In the article we discuss the various sources of compression and
present a model that accurately describes data measured during tests performed
with individual radiomeric chains. After discussing test results we present the
best parameter set representing the receiver response and discuss the impact of
non linearity on in-flight calibration, which is shown to be negligible.Comment: this paper is part of the Prelaunch status LFI papers published on
JINST: http://www.iop.org/EJ/journal/-page=extra.proc5/jinst; This is an
author-created, un-copyedited version of an article accepted for publication
in JINST. IOP Publishing Ltd is not responsible for any errors or omissions
in this version of the manuscript or any version derived from it. The
definitive publisher authenticated version is available online at
10.1088/1748-0221/4/12/T12011
Investigating serum and tissue expression identified a cytokine/chemokine signature as a highly effective melanoma marker
The identification of reliable and quantitative melanoma biomarkers may help an early diagnosis and may directly affect melanoma mortality and morbidity. The aim of the present study was to identify effective biomarkers by investigating the expression of 27 cytokines/chemokines in melanoma compared to healthy controls, both in serum and in tissue samples. Serum samples were from 232 patients recruited at the IDI-IRCCS hospital. Expression was quantified by xMAP technology, on 27 cytokines/chemokines, compared to the control sera. RNA expression data of the same 27 molecules were obtained from 511 melanoma-and healthy-tissue samples, from the GENT2 database. Statistical analysis involved a 3-step approach: analysis of the single-molecules by Mann–Whitney analysis; analysis of paired-molecules by Pearson correlation; and profile analysis by the machine learning algorithm Support Vector Machine (SVM). Single-molecule analysis of serum expression identified IL-1b, IL-6, IP-10, PDGF-BB, and RANTES differently expressed in melanoma (p < 0.05). Expression of IL-8, GM-CSF, MCP-1, and TNF-α was found to be significantly correlated with Breslow thickness. Eotaxin and MCP-1 were found differentially expressed in male vs. female patients. Tissue expression analysis identified very effective marker/predictor genes, namely, IL-1Ra, IL-7, MIP-1a, and MIP-1b, with individual AUC values of 0.88, 0.86, 0.93, 0.87, respectively. SVM analysis of the tissue expression data identified the combination of these four molecules as the most effective signature to discriminate melanoma patients (AUC = 0.98). Validation, using the GEPIA2 database on an additional 1019 independent samples, fully confirmed these observations. The present study demonstrates, for the first time, that the IL-1Ra, IL-7, MIP-1a, and MIP-1b gene signature discriminates melanoma from control tissues with extremely high efficacy. We therefore propose this 4-molecule combination as an effective melanoma marker
Design, development and verification of the 30 and 44 GHz front-end modules for the Planck Low Frequency Instrument
We give a description of the design, construction and testing of the 30 and
44 GHz Front End Modules (FEMs) for the Low Frequency Instrument (LFI) of the
Planck mission to be launched in 2009. The scientific requirements of the
mission determine the performance parameters to be met by the FEMs, including
their linear polarization characteristics.
The FEM design is that of a differential pseudo-correlation radiometer in
which the signal from the sky is compared with a 4-K blackbody load. The Low
Noise Amplifier (LNA) at the heart of the FEM is based on indium phosphide High
Electron Mobility Transistors (HEMTs). The radiometer incorporates a novel
phase-switch design which gives excellent amplitude and phase match across the
band.
The noise temperature requirements are met within the measurement errors at
the two frequencies. For the most sensitive LNAs, the noise temperature at the
band centre is 3 and 5 times the quantum limit at 30 and 44 GHz respectively.
For some of the FEMs, the noise temperature is still falling as the ambient
temperature is reduced to 20 K. Stability tests of the FEMs, including a
measurement of the 1/f knee frequency, also meet mission requirements.
The 30 and 44 GHz FEMs have met or bettered the mission requirements in all
critical aspects. The most sensitive LNAs have reached new limits of noise
temperature for HEMTs at their band centres. The FEMs have well-defined linear
polarization characteristcs.Comment: 39 pages, 33 figures (33 EPS files), 12 tables. Planck LFI technical
papers published by JINST:
http://www.iop.org/EJ/journal/-page=extra.proc5/1748-022
Advanced modelling of the Planck-LFI radiometers
The Low Frequency Instrument (LFI) is a radiometer array covering the 30-70
GHz spectral range on-board the ESA Planck satellite, launched on May 14th,
2009 to observe the cosmic microwave background (CMB) with unprecedented
precision. In this paper we describe the development and validation of a
software model of the LFI pseudo-correlation receivers which enables to
reproduce and predict all the main system parameters of interest as measured at
each of the 44 LFI detectors. These include system total gain, noise
temperature, band-pass response, non-linear response. The LFI Advanced RF Model
(LARFM) has been constructed by using commercial software tools and data of
each radiometer component as measured at single unit level. The LARFM has been
successfully used to reproduce the LFI behavior observed during the LFI
ground-test campaign. The model is an essential element in the database of LFI
data processing center and will be available for any detailed study of
radiometer behaviour during the survey.Comment: 21 pages, 15 figures, this paper is part of the Prelaunch status LFI
papers published on JINST:
http://www.iop.org/EJ/journal/-page=extra.proc5/jins
Optimization of Planck/LFI on--board data handling
To asses stability against 1/f noise, the Low Frequency Instrument (LFI)
onboard the Planck mission will acquire data at a rate much higher than the
data rate allowed by its telemetry bandwith of 35.5 kbps. The data are
processed by an onboard pipeline, followed onground by a reversing step. This
paper illustrates the LFI scientific onboard processing to fit the allowed
datarate. This is a lossy process tuned by using a set of 5 parameters Naver,
r1, r2, q, O for each of the 44 LFI detectors. The paper quantifies the level
of distortion introduced by the onboard processing, EpsilonQ, as a function of
these parameters. It describes the method of optimizing the onboard processing
chain. The tuning procedure is based on a optimization algorithm applied to
unprocessed and uncompressed raw data provided either by simulations, prelaunch
tests or data taken from LFI operating in diagnostic mode. All the needed
optimization steps are performed by an automated tool, OCA2, which ends with
optimized parameters and produces a set of statistical indicators, among them
the compression rate Cr and EpsilonQ. For Planck/LFI the requirements are Cr =
2.4 and EpsilonQ <= 10% of the rms of the instrumental white noise. To speedup
the process an analytical model is developed that is able to extract most of
the relevant information on EpsilonQ and Cr as a function of the signal
statistics and the processing parameters. This model will be of interest for
the instrument data analysis. The method was applied during ground tests when
the instrument was operating in conditions representative of flight. Optimized
parameters were obtained and the performance has been verified, the required
data rate of 35.5 Kbps has been achieved while keeping EpsilonQ at a level of
3.8% of white noise rms well within the requirements.Comment: 51 pages, 13 fig.s, 3 tables, pdflatex, needs JINST.csl, graphicx,
txfonts, rotating; Issue 1.0 10 nov 2009; Sub. to JINST 23Jun09, Accepted
10Nov09, Pub.: 29Dec09; This is a preprint, not the final versio
Off-line radiometric analysis of Planck/LFI data
The Planck Low Frequency Instrument (LFI) is an array of 22
pseudo-correlation radiometers on-board the Planck satellite to measure
temperature and polarization anisotropies in the Cosmic Microwave Background
(CMB) in three frequency bands (30, 44 and 70 GHz). To calibrate and verify the
performances of the LFI, a software suite named LIFE has been developed. Its
aims are to provide a common platform to use for analyzing the results of the
tests performed on the single components of the instrument (RCAs, Radiometric
Chain Assemblies) and on the integrated Radiometric Array Assembly (RAA).
Moreover, its analysis tools are designed to be used during the flight as well
to produce periodic reports on the status of the instrument. The LIFE suite has
been developed using a multi-layered, cross-platform approach. It implements a
number of analysis modules written in RSI IDL, each accessing the data through
a portable and heavily optimized library of functions written in C and C++. One
of the most important features of LIFE is its ability to run the same data
analysis codes both using ground test data and real flight data as input. The
LIFE software suite has been successfully used during the RCA/RAA tests and the
Planck Integrated System Tests. Moreover, the software has also passed the
verification for its in-flight use during the System Operations Verification
Tests, held in October 2008.Comment: Planck LFI technical papers published by JINST:
http://www.iop.org/EJ/journal/-page=extra.proc5/1748-022
Synchronous Optical and Radio Polarization Variability in the Blazar OJ287
We explore the variability and cross-frequency correlation of the flux
density and polarization of the blazar OJ287, using imaging at 43 GHz with the
Very Long Baseline Array, as well as optical and near-infrared polarimetry. The
polarization and flux density in both the optical waveband and the 43 GHz
compact core increased by a small amount in late 2005, and increased
significantly along with the near-IR polarization and flux density over the
course of 10 days in early 2006. Furthermore, the values of the electric vector
position angle (EVPA) at the three wavebands are similar. At 43 GHz, the EVPA
of the blazar core is perpendicular to the flow of the jet, while the EVPAs of
emerging superluminal knots are aligned parallel to the jet axis. The core
polarization is that expected if shear aligns the magnetic field at the
boundary between flows of disparate velocities within the jet. Using variations
in flux density, percentage polarization, and EVPA, we model the inner jet as a
spine-sheath system. The model jet contains a turbulent spine of half-width 1.2
degrees and maximum Lorentz factor of 16.5, a turbulent sheath with Lorentz
factor of 5, and a boundary region of sheared field between the spine and
sheath. Transverse shocks propagating along the fast, turbulent spine can
explain the superluminal knots. The observed flux density and polarization
variations are then compatible with changes in the direction of the inner jet
caused by a temporary change in the position of the core if the spine contains
wiggles owing to an instability. In addition, we can explain a stable offset of
optical and near-IR percentage polarization by a steepening of spectral index
with frequency, as supported by the data.Comment: 34 pages, 12 figures; To be published in Astrophysical Journal,
accepted 03/200
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