Diamond nucleation from the gas phase onto cold-worked Co-cemented tungsten carbide

Co-cemented tungsten carbide (WC–Co) substrates with fine (1 μm) and coarse (6 μm) grain size were sintered using 6 wt.% Co as a binder. The as-sintered samples were ground to the final geometry (10×10×3 mm3). After the grinding treatment, the full width at half maximum (FWHM) of the WC X-ray diffraction (XRD) peaks indicated a high level of strain in a few micrometers thick surface layer, according to the penetration depth of Cu Kα radiation. The as-ground substrates were submitted to a two-step etching procedure with Murakami's solution, to roughen the surface, and 10 s acid wash to etch surface cobalt out. The Murakami's etching time was varied between 1 and 20 min. Fine- and coarse-grained substrates submitted to different chemical etching times were characterized by scanning electron microscopy and XRD, and then submitted to short diamond nucleation runs in a Hot Filament Chemical Vapour Deposition reactor. Both FWHM of WC peaks and diamond nucleation density decreased by increasing the Murakami's etching duration, providing that the etched layer did not exceed 2 μm thickness. When a layer thicker than a couple of micrometers was removed by etching, diamond nucleation density was very low and no more dependent on etching time. This occurrence suggested that diamond nucleation density correlates well with the amount of residual strain at the substrate surface and can be tailored by a suitable control of strain-related defects produced by mechanical treatments

Dry turning of alumina/aluminum composites with CVD diamond coated Co-cemented tungsten carbide tools

Triangular (TPGN 160308) WC-6 wt.%Co inserts having different average grain sizes (1 and 3 µm) were submitted to surface roughening either by wet etching with Murakami's reagent or by a heat treatment in the hot filament chemical vapour deposition (HFCVD) reactor. The heat treatment was performed in a monohydrogen-rich atmosphere at substrate temperatures as high as 1000 degrees C. Scanning electron microscopy and energy-dispersive spectroscopy showed that this pre-treatment led to surface roughening of the as-ground inserts and to a lower surface Co concentration. Prior to deposition, all inserts were etched with an acid solution of hydrogen peroxide. Diamond coatings were deposited by HFCVD. The coated inserts were tested by dry machining of aluminum-matrix composite (Al-10%Al2O3) bars. Turning test results indicated that a proper combination of substrate pretreatment and microstructure can significantly improve tool life

Dark Matter searches using gravitational wave bar detectors: quark nuggets and newtorites

Many experiments have searched for supersymmetric WIMP dark matter, with null results. This may suggest to look for more exotic possibilities, for example compact ultra-dense quark nuggets, widely discussed in literature with several different names. Nuclearites are an example of candidate compact objects with atomic size cross section. After a short discussion on nuclearites, the result of a nuclearite search with the gravitational wave bar detectors Nautilus and Explorer is reported. The geometrical acceptance of the bar detectors is 19.5 $\rm m^2$ sr, that is smaller than that of other detectors used for similar searches. However, the detection mechanism is completely different and is more straightforward than in other detectors. The experimental limits we obtain are of interest because, for nuclearites of mass less than $10^{-5}$ g, we find a flux smaller than that one predicted considering nuclearites as dark matter candidates. Particles with gravitational only interactions (newtorites) are another example. In this case the sensitivity is quite poor and a short discussion is reported on possible improvements.Comment: published on Astroparticle Physics Sept 25th 2016 replaced fig 1

Genetic variability among Blastoschizomyces capitatus isolates from different clinical sources.

Sixteen clinical isolates and nine ATCC reference strains of Blastoschizomyces capitatus were analysed genetically, examined for the cellobiose, arbutin and salicin assimilation and tested for the aspartyl-proteinase secretion. The restriction endonuclease analysis (REA) with HpaII and HinfI enzymes and the electrophoretic karyotype (EK) were investigated. Both the restriction enzymes revealed two groups (I, II) constituted by the same isolates: 17 isolates (68%) in group I and 8 (32%) in group II. The EK analysis revealed sixteen groups. These data prompts for a genetic variability of the isolates of Blastoschizomyces capitatus and their account in two distinct genetic groups as suggested by REA. This grouping was confirmed by examing the utilisation of cellobiose, arbutin and salicin. The tests for secretory aspartyl proteinase (Sap) were positive only for three isolates, suggesting a marginal role of this specific enzyme in pathogenesis for these isolates

Quark nuggets search using 2350 Kg gravitational waves aluminum bar detectors

The gravitational wave resonant detectors can be used as detectors of quark nuggets, like nuclearites (nuclear matter with a strange quark). This search has been carried out using data from two 2350 Kg, 2 K cooled, aluminum bar detectors: NAUTILUS, located in Frascati (Italy), and EXPLORER, that was located in CERN Geneva (CH). Both antennas are equipped with cosmic ray shower detectors: signals in the bar due to showers are continuously detected and used to characterize the antenna performances. The bar excitation mechanism is based on the so called thermo-acoustic effect, studied on dedicated experiments that use particle beams. This mechanism predicts that vibrations of bars are induced by the heat deposited in the bar from the particle. The geometrical acceptance of the bar detectors is 19.5 $\rm m^2$ sr, that is smaller than that of other detectors used for similar searches. However, the detection mechanism is completely different and is more straightforward than in other detectors. We will show the results of ten years of data from NAUTILUS (2003-2012) and 7 years from EXPLORER (2003-2009). The experimental limits we obtain are of interest because, for nuclearites of mass less than $10^{-4}$ grams, we find a flux smaller than that one predicted considering nuclearites as dark matter candidates.Comment: presented to the 33rd International Cosmic Ray Conference Rio de Janeiro 201

Amniotic fluid absorption and growth functions in humans: what can we indirectly learn from congenital digestive atresias?

Background: Amniotic fluid (AF) was thought of just as a mechanical cushioning to the foetus. Nowadays, its role during pregnancy is getting more attention, suggesting hitherto unknown aspects. The aim of the study is to speculate on AF nutritional functions and its clinical repercussions based on what digestive tract (DT) atresias seem to suggest. Methods: A retrospective analysis of the patients admitted to our department for DT atresias between 2000 and 2020 was conducted. Patients’ birth weight (BW), gestational age (GA) at birth and diagnosis were recorded. The following were excluded from the study: oesophageal atresias (OA) with tracheoesophageal fistula (TOF), colonic and anal atresias and patients with associated major comorbidities. A control group was made of patients admitted to our ward in the same period for congenital pulmonary airway malformations (CPAM). To standardize the BW, it was coupled with birth GA calculating the newborn percentiles. The mean newborn percentiles of OAs, duodenal atresias (DAs), jejunal atresias (JAs), and ileal atresias (IAs) were independently compared with the control group using Student’s t-test. Lastly, the significance of the frequencies’ distribution of newborns born small for gestational age (SGA) between the DT atresias and the control group was evaluated with the χ2 test, and the OR were calculated. A p-value < 0.05 was considered statistically significant. Results: A total of 231 patients were eligible for the study: 36 OAs without TOF, mean BW 2488.8 ± 491 g (range 1630–3750 g), mean GA 36.8 ± 2.1 weeks (31–40 weeks), mean newborn percentile 18 ± 22 (1–75); 20 DAs, mean BW 2586.8 ± 577.9 g (1250–3462 g), mean GA 36.2 ± 2.5 weeks (31–40 weeks), mean newborn percentile 31 ± 23 (3–79); 17 JAs, mean BW 2483.5 ± 621.7 g (900–3205 g), mean GA 34.8 ± 2.1 weeks (30–38 weeks), mean newborn percentile 44 ± 28 (4–96); 17 IAs, mean BW 2646.1 ± 769.8 g (1162.0–3888 g), mean GA 35.9 ± 3.2 weeks (30–41 weeks), mean newborn percentile 44 ± 26 (1–82); and 141 CPAMs with mean BW 3287.4 ± 492.0 g (980–4580 g), mean GA 38.7 ± 1.8 weeks (26–41 weeks), mean newborn percentile 43 ± 26 (1–99). The number of SGA neonates was 18 between OA patients (50%), 4 between DAs (20%), 1 between JAs (6%), 2 between IAs (12%) and 11 between CPAMs (8%). The mean percentile of the OAs and DAs was lower than the control group with a p of < .01 and .03 while no statistical significance was found in the comparison between DAs, JAs, IAs and CPAMs with a p of .06, .86 and .59. The incidence of SGA in the control group resulted lower than the one in the DT atresias where it becomes higher the more proximal the atresia is (p < .05). The OR of SGA newborn in the OA group was 11.8, in DA 3.0, in JA 0.7 and in IA 1.6. Conclusion: AF showed to have a great impact on foetal growth, and its preferred site of absorption seemed to be the stomach and duodenum. Its nutritional role could have an important predictive value in diagnosing DT atresia both prenatally and postnatally

Determining the Extent of Soil Degradation Processes Using Trend Analyses at a Regional Multispectral Scale

In order to ensure the sustainability of production from agricultural lands, the degradation processes surrounding the fertile land environment must be monitored. Human-induced risk and status of soil degradation (SD) were assessed in the Northern-Eastern part of the Nile delta using trend analyses for years 2013 to 2023. SD hotspot areas were identified using time-series analysis of satellite-derived indices as a small fraction of the difference between the observed indices and the geostatistical analyses projected from the soil data. The method operated on the assumption that the negative trend of photosynthetic capacity of plants is an indicator of SD independently of climate variability. Combinations of soil, water, and vegetation’s indices were integrated to achieve the goals of the study. Thirteen soil profiles were dug in the hotspots areas. The soil was affected by salinity and alkalinity risks ranging from slight to strong, while compaction and waterlogging ranged from slight to moderate. According to the GIS-model results, 30% of the soils were subject to slight degradation threats, 50% were subject to strong risks, and 20% were subject to moderate risks. The primary human-caused sources of SD are excessive irrigation, poor conservation practices, improper utilisation of heavy machines, and insufficient drainage. Electrical conductivity (EC), exchangeable soil percentage (ESP), bulk density (BD), and water table depth were the main causes of SD in the area. Generally, chemical degradation risks were low, while physical risks were very high in the area. Trend analyses of remote sensing indices (RSI) proved to be effective and accurate tools to monitor environmental dynamic changes. Principal components analyses were used to compare and prioritise among the used RSI. RSI pixel-wise residual trend indicated SD areas were related to soil data. The spatial and temporal trends of the indices in the region followed the patterns of drought, salinity, soil moisture, and the difficulties in separating the impacts of drought and submerged on SD on vegetation photosynthetic capacity. Therefore, future studies of land degradation and desertification should proceed using indices as a factor predictor of SD analysis

Interleukin-30 feeds breast cancer stem cells via CXCL10 and IL23 autocrine loops and shapes immune contexture and host outcome

Background Breast cancer (BC) progression to metastatic disease is the leading cause of death in women worldwide. Metastasis is driven by cancer stem cells (CSCs) and signals from their microenvironment. Interleukin (IL) 30 promotes BC progression, and its expression correlates with disease recurrence and mortality. Whether it acts by regulating BCSCs is unknown and could have significant therapeutic implications. Methods Human (h) and murine (m) BCSCs were tested for their production of and response to IL30 by using flow cytometry, confocal microscopy, proliferation and sphere-formation assays, and PCR array. Immunocompetent mice were used to investigate the role of BCSC-derived IL30 on tumor development and host outcome. TCGA PanCancer and Oncomine databases provided gene expression data from 1084 and 75 hBC samples, respectively, and immunostaining unveiled the BCSC microenvironment. Results hBCSCs constitutively expressed IL30 as a membrane-anchored glycoprotein. Blocking IL30 hindered their proliferation and self-renewal efficiency, which were boosted by IL30 overexpression. IL30 regulation of immunity gene expression in human and murine BCSCs shared a significant induction of IL23 and CXCL10. Both immunoregulatory mediators stimulated BCSC proliferation and self-renewal, while their selective blockade dramatically hindered IL30-dependent BCSC proliferation and mammosphere formation. Orthotopic implantation of IL30-overexpressing mBCSCs, in syngeneic mice, gave rise to poorly differentiated and highly proliferating MYC + KLF4 + LAG3 + tumors, which expressed CXCL10 and IL23, and were infiltrated by myeloid-derived cells, Foxp3 + T regulatory cells and NKp46 + RORÎ 3t + type 3 innate lymphoid cells, resulting in increased metastasis and reduced survival. In tumor tissues from patients with BC, expression of IL30 overlapped with that of CXCL10 and IL23, and ranked beyond the 95th percentile in a Triple-Negative enriched BC collection from the Oncomine Platform. CIBERSORTx highlighted a defective dendritic cell, CD4 + T and Î 3δT lymphocyte content and a prominent LAG3 expression in IL30 high versus IL30 low human BC samples from the TCGA PanCancer collection. Conclusions Constitutive expression of membrane-bound IL30 regulates BCSC viability by juxtacrine signals and via second-level mediators, mainly CXCL10 and IL23. Their autocrine loops mediate much of the CSC growth factor activity of IL30, while their paracrine effect contributes to IL30 shaping of immune contexture. IL30-related immune subversion, which also emerged from computational analyses, strongly suggests that targeting IL30 can restrain the BCSC compartment and counteract BC progression

Soil Salinity Assessing and Mapping Using Several Statistical and Distribution Techniques in Arid and Semi-Arid Ecosystems, Egypt

Oasis lands in Egypt are commonly described as salty soils; therefore, waterlogging and higher soil salinity are major obstacles to sustainable agricultural development. This study aims to map and assess soil salinization at El-Farafra Oasis in the Egypt Western Desert based on salinity indices, Imaging Spectroscopy (IS), and statistical techniques. The regression model was developed to test the relationship between the electrical conductivity (ECe) of 70 surface soil samples and seven salinity indices (SI 1, SI 2, SI 5, SI 6, SI 7, SI 8, and SI 9) to produce soil salinity maps depending on Landsat-8 (OLI) images. The investigations of soil salinization and salinity indices were validated in a studied area based on 30 soil samples; the obtained results represented that all salinity indices have shown satisfactory correlations between ECe values for each soil sample site and salinity indices, except for the SI 5 index that present non-significant correlations with R2 value of 0.2688. The SI 8 index shows a higher negative significant correlation with ECe and an R2 value of 0.6356. There is a significant positive correlation at the (p < 0.01) level between SI 9 and ECe (r = 0.514), a non-significant correlation at the (p < 0.05) level between soil ECe and SI 1 index (r = 0.495), and the best-verified salinity index was for SI 7 that has a low estimated RMSE error of 8.58. Finally, the highest standard error (R2) was represented as ECe (dS m−1) with an R2 of 0.881, and the lowest one was SI 9 with an R2 of 0.428, according to Tukey’s test analysis. Therefore, observing and investigating soil salinity are essential requirements for appropriate natural resource management plans in the future

A population approach to characterise amisulpride pharmacokinetics in older people and Alzheimer's disease

Introduction Current prescribing guidelines for the antipsychotic amisulpride are based largely on pharmacokinetic (PK) studies in young adults, and there is a relative absence of data on older patients, who are at greatest risk of developing adverse events. Methods This study aimed to develop a population PK model for amisulpride specifically in older people, by combining data from a richly sampled phase 1, single (50 mg) dose study in healthy older people (n = 20, 65–79 years), with a clinical dataset obtained during off label, low-dose (25–75 mg daily) amisulpride prescribing in older people with Alzheimer’s disease (AD) (n = 25, 69–92 years), as part of an observational study. Results After introducing a scaling factor based on body weight, age accounted for 20 % of the inter-individual variability in drug clearance (CL), resulting in a 54 % difference in CL between those aged 65 and those aged 85 years, and higher blood concentrations in older patients. Discussion These findings argue for the consideration of age and weight-based dose stratification to optimise amisulpride prescribing in older people, particularly in those aged 85 years and above