A longitudinal study of intonation in an a cappella singing quintet

Objective The skill to control pitch accurately is an important feature of performance in singing ensembles as it boosts musical excellence. Previous studies analyzing single performance sessions provide inconclusive and contrasting results on whether singers in ensembles tend to use a tuning system which deviates from equal temperament for their intonation. The present study observes the evolution of intonation in a newly formed student singing quintet during their first term of study. Methods/Design A semiprofessional singing quintet was recorded using head-worn microphones and electrolaryngograph electrodes to allow fundamental frequency (fo) evaluation of the individual voices. In addition, a camcorder was used to record verbal interactions between singers. The ensemble rehearsed a homophonic piece arranged for the study during five rehearsal sessions over four months. Singers practiced the piece for 10 minutes in each rehearsal, and performed three repetitions of the same pieces pre-rehearsal and post-rehearsal. Audio and electrolaryngograph data of the repeated performances, and video recordings of the rehearsals were analyzed. Aspects of intonation were then measured by extracting the fo values from the electrolaryngograph and acoustic signal, and compared within rehearsals (pre and post) and between rehearsals (rehearsals 1 to 5), and across repetitions (take 1 to 3). Time-stamped transcriptions of rehearsal discussions were used to identify verbal interactions related to tuning, the tuning strategies adopted, and their location (bar or chord) within the piece. Results/Discussion Tuning of each singer was closer to equal temperament than just intonation, but the size of major thirds was slightly closer to just intonation, and minor thirds closer to equal temperament. These findings were consistent within and between rehearsals, and across repetitions. Tuning was highlighted as an important feature of rehearsal during the study term, and a range of strategies were adopted to solve tuning related issues. This study provides a novel holistic assessment of tuning strategies within a singing ensemble, furthering understanding of performance practices as well as revealing the complex approach needed for future research in this area. These findings are particularly important for directors and singers to tailor rehearsal strategies that address tuning in singing ensembles, showing that approaches need to be context driven rather than based on theoretical ideal

A Longitudinal Study Investigating Synchronization in a Singing Quintet

Research suggests that synchronization between musicians during ensemble performances can be affected by the rhythmic or tonal complexity of the piece being performed and by group roles such as leader-follower relationships. Since previous studies have mostly been conducted within single performance sessions, developmental aspects of interpersonal synchronization in ensembles remain underinvestigated. This longitudinal study followed a newly formed singing ensemble from initial rehearsals to the performance stage in order to investigate the evolution of synchronization between advanced singing students during a university term of study in relation to the musical content of the piece and leader-follower relationships. An advanced postgraduate singing quintet was recorded using head-worn microphones and laryngograph electrodes to allow fundamental frequency evaluation of the individual voices. The quintet, formed to complete a 1-year Master’s programme in ensemble singing, rehearsed two pieces composed for the study, during five rehearsals over 3 months. Singers practised the same pieces in a randomized order across rehearsals and performed three repetitions of the same pieces before and after each rehearsal, resulting in six recordings per piece/rehearsal. Audio and laryngograph data of the repeated performances were collected, and synchronization was measured by extracting note times from the fundamental frequency values. The asynchronies of the two pieces before and after rehearsals were calculated and compared both within rehearsals (pre and post) and between rehearsals (rehearsals 1-5). Results demonstrate an increase in the precision of synchronization over the course of study, depending on the piece being rehearsed, and a more variable synchronization for the more rhythmically complex piece. Results also show changes in the distribution of the tendency to precede all co-performers across rehearsals, which became equally distributed among the musicians during the last rehearsal. The results reported here could have important implications for the tailoring of rehearsal strategies that could improve interpersonal synchronization between musicians during ensemble performances

Perception of synchronization in singing ensembles

Recent investigations analysing synchronization in singing ensembles have shown that the precision of synchronization during singing duo performances is better in the presence of visual contact between the singers than without. Research has also shown that synchronisation improves with practice across rehearsals in a newly formed singing quintet. However, whether listeners’ perceptions of synchronization reflect the different patterns of synchronization that were observed during ensemble performance with and without visual contact and across rehearsals has not yet been investigated. This study aims to analyse the perception of the synchronization associated with altered visual contact and amount of rehearsal during singing duo and quintet performances respectively, for listeners with different levels of musical expertise. A set of fifty-eight singing recordings selected from duo and quintet ensemble performances, collected from previous investigations of interpersonal synchrony, was presented to 33 listeners, including non-experts (university students with little or no music training), performers in the group (singers who performed the pieces used for the study), and other musicians (advanced music students). Participants were required to listen to each trial and judge the level of “togetherness” on a sliding scale from zero to 100. Results show that listeners, irrespective of their musical training and performance experience, perceived differences in the synchronization in the duo tokens depending on the presence/absence of visual contact between singers; on the other hand, the smaller asynchrony patterns measured across rehearsals in the singing quintet recordings were not perceived. This study contributes to our understanding of perceptions of synchronization by individuals with different levels of musical expertise, and underscores the perceptual salience of synchronization, regardless of musical experience

Circulating sST2 and catestatin levels in patients with acute worsening of heart failure: a report from the CATSTAT‐HF study

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Microvascular Dysfunction in Heart Failure With Preserved Ejection Fraction

Heart failure with preserved ejection fraction (HFpEF) is an increasingly studied entity accounting for 50% of all diagnosed heart failure and that has claimed its own dignity being markedly different from heart failure with reduced EF in terms of etiology and natural history (Graziani et al., 2018). Recently, a growing body of evidence points the finger toward microvascular dysfunction as the major determinant of the pathological cascade that justifies clinical manifestations (Crea et al., 2017). The high burden of comorbidities such as metabolic syndrome, hypertension, atrial fibrillation, chronic kidney disease, obstructive sleep apnea, and similar, could lead to a systemic inflammatory state that impacts the physiology of the endothelium and the perivascular environment, engaging complex molecular pathways that ultimately converge to myocardial fibrosis, stiffening, and dysfunction (Paulus and Tschope, 2013). These changes could even self-perpetrate with a positive feedback where hypoxia and locally released inflammatory cytokines trigger interstitial fibrosis and hypertrophy (Ohanyan et al., 2018). Identifying microvascular dysfunction both as the cause and the maintenance mechanism of this condition has opened the field to explore specific pharmacological targets like nitric oxide (NO) pathway, sarcomeric titin, transforming growth factor beta (TGF-β) pathway, immunomodulators or adenosine receptors, trying to tackle the endothelial impairment that lies in the background of this syndrome (Graziani et al., 2018;Lam et al., 2018). Yet, many questions remain, and the new data collected still lack a translation to improved treatment strategies. To further elaborate on this tangled and exponentially growing topic, we will review the evidence favoring a microvasculature-driven etiology of this condition, its clinical correlations, the proposed diagnostic workup, and the available/hypothesized therapeutic options to address microvascular dysfunction in the failing heart

Indication, from Pioneer 10/11, Galileo, and Ulysses Data, of an Apparent Anomalous, Weak, Long-Range Acceleration

Radio metric data from the Pioneer 10/11, Galileo, and Ulysses spacecraft indicate an apparent anomalous, constant, acceleration acting on the spacecraft with a magnitude $\sim 8.5\times 10^{-8}$ cm/s$^2$, directed towards the Sun. Two independent codes and physical strategies have been used to analyze the data. A number of potential causes have been ruled out. We discuss future kinematic tests and possible origins of the signal.Comment: Revtex, 4 pages and 1 figure. Minor changes for publicatio

an outbreak of severe invasive meningococcal disease due to a capsular switched neisseria meningitidis hypervirulent strain b cc11

Abstract Objectives The aim was to investigate an outbreak of invasive meningococcal disease (IMD) in Southern Sardinia. Methods Epidemiological and microbiological investigations were performed. The latter included antimicrobial susceptibility testing and whole-genome sequencing (WGS). Results Seven individuals with severe IMD were found to be infected with serogroup B (MenB) Neisseria meningitidis in the first quarter of 2018. Five of the seven cases (five males; mean age 19 years; range 18–21 years; CFR 40%) were due to a unique strain B:P1.5-1,10-8:F3-6:ST-11(cc11), probably switched from the hypervirulent C-cc11, as confirmed by WGS. All five patients had attended the same nightclub in the 2 weeks prior to symptom onset. Public health measures, including chemoprophylaxis of contacts and active immunization against MenB, were implemented. Conclusions We observed five IMD cases due to the same switched MenB strain. The hypervirulent B:P1.5-1,10-8:F3-6:ST-11(cc11) strain, probably switched from C-cc11, is of concern due to the observed high virulence and case fatality rates. All the patients shared the same place of probable exposure. The molecular characterization of the invasive strain allowed the outbreak to be confirmed, which was then controlled through timely public health action

An outbreak of severe invasive meningococcal disease due to a capsular switched Neisseria meningitidis hypervirulent strain B:cc11

Objectives: The aim was to investigate an outbreak of invasive meningococcal disease (IMD) in Southern Sardinia. Methods: Epidemiological and microbiological investigations were performed. The latter included antimicrobial susceptibility testing and whole-genome sequencing (WGS). Results: Seven individuals with severe IMD were found to be infected with serogroup B (MenB) Neisseria meningitidis in the first quarter of 2018. Five of the seven cases (five males; mean age 19 years; range 18–21 years; CFR 40%) were due to a unique strain B:P1.5-1,10-8:F3-6:ST-11(cc11), probably switched from the hypervirulent C-cc11, as confirmed by WGS. All five patients had attended the same nightclub in the 2 weeks prior to symptom onset. Public health measures, including chemoprophylaxis of contacts and active immunization against MenB, were implemented. Conclusions: We observed five IMD cases due to the same switched MenB strain. The hypervirulent B:P1.5-1,10-8:F3-6:ST-11(cc11) strain, probably switched from C-cc11, is of concern due to the observed high virulence and case fatality rates. All the patients shared the same place of probable exposure. The molecular characterization of the invasive strain allowed the outbreak to be confirmed, which was then controlled through timely public health action

One year of Galileo dust data from the Jovian system: 1996

The dust detector system onboard Galileo records dust impacts in circumjovian space since the spacecraft has been injected into a bound orbit about Jupiter in December 1995. This is the sixth in a series of papers dedicated to presenting Galileo and Ulysses dust data. We present data from the Galileo dust instrument for the period January to December 1996 when the spacecraft completed four orbits about Jupiter (G1, G2, C3 and E4). Data were obtained as high resolution realtime science data or recorded data during a time period of 100 days, or via memory read-outs during the remaining times. Because the data transmission rate of the spacecraft is very low, the complete data set (i. e. all parameters measured by the instrument during impact of a dust particle) for only 2% (5353) of all particles detected could be transmitted to Earth; the other particles were only counted. Together with the data for 2883 particles detected during Galileo's interplanetary cruise and published earlier, complete data of 8236 particles detected by the Galileo dust instrument from 1989 to 1996 are now available. The majority of particles detected are tiny grains (about 10 nm in radius) originating from Jupiter's innermost Galilean moon Io. These grains have been detected throughout the Jovian system and the highest impact rates exceeded $\rm 100 min^{-1}$. A small number of grains has been detected in the close vicinity of the Galilean moons Europa, Ganymede and Callisto which belong to impact-generated dust clouds formed by (mostly submicrometer sized) ejecta from the surfaces of the moons (Kr\"uger et al., Nature, 399, 558, 1999). Impacts of submicrometer to micrometer sized grains have been detected thoughout the Jovian system and especially in the region between the Galilean moons.Comment: accepted for Planetary and Space Science, 33 pages, 6 tables, 10 figure

Dapagliflozin treatment is associated with a reduction of epicardial adipose tissue thickness and epicardial glucose uptake in human type 2 diabetes

Objective: We recently demonstrated that treatment with sodium-glucose cotransporter-2 inhibitors (SGLT-2i) leads to an increase in myocardial flow reserve in patients with type 2 diabetes (T2D) with stable coronary artery disease (CAD). The mechanism by which this occurs is, however, unclear. One of the risk factors for cardiovascular disease is inflammation of epicardial adipose tissue (EAT). Since the latter is often increased in type 2 diabetes patients, it could play a role in coronary microvascular dysfunction. It is also well known that SGLT-2i modify adipose tissue metabolism. We aimed to investigate the effects of the SGLT-2i dapagliflozin on metabolism and visceral and subcutaneous adipose tissue thickness in T2D patients with stable coronary artery disease and to verify whether these changes could explain observed changes in myocardial flow. Methods: We performed a single-center, prospective, randomized, double-blind, controlled clinical trial with 14 T2D patients randomized 1:1 to SGLT-2i dapagliflozin (10 mg daily) or placebo. The thickness of visceral (epicardial, mediastinal, perirenal) and subcutaneous adipose tissue and glucose uptake were assessed at baseline and 4 weeks after treatment initiation by 2-deoxy-2-[18F]fluoro-D-glucose Positron Emission Tomography/Computed Tomography during hyperinsulinemic euglycemic clamp. Results: The two groups were well-matched for baseline characteristics (age, diabetes duration, HbA1c, BMI, renal and heart function). Dapagliflozin treatment significantly reduced EAT thickness by 19% (p = 0.03). There was a significant 21.6% reduction in EAT glucose uptake during euglycemic hyperinsulinemic clamp in the dapagliflozin group compared with the placebo group (p = 0.014). There were no significant effects on adipose tissue thickness/metabolism in the other depots explored. Conclusions: SGLT-2 inhibition selectively reduces EAT thickness and EAT glucose uptake in T2D patients, suggesting a reduction of EAT inflammation. This could explain the observed increase in myocardial flow reserve, providing new insights into SGLT-2i cardiovascular benefits