15 research outputs found
Climate oscillations and changes in freshwater budget of the central-eastern Mediterranean between 20,000 and 70,000 years ago
Clinical and environmental distribution of legionella pneumophila in a university hospital in italy: efficacy of ultraviolet disinfection
The molecular epidemiology of Legionella pneumophila in the 'V. Monaldi' University Hospital was studied. Seven cases of nosocomial Legionnaires' disease were diagnosed between 1999 and 2003. Two clinical legionella strains obtained from two patients in the adult cardiac surgery unit (CSU) and 30 environmental legionella strains from the paediatric and adult CSUs, neonatal intensive care unit (NICU) and the cardiorespiratory intensive care unit (CR-ICU) were serotyped and genotyped. L. pneumophila serogroup 1/Philadelphia with an identical pulsed-field gel electrophoresis (PFGE) profile A was isolated from two patients in the adult CSU, and from three and one water samples taken in the adult CSU and the paediatric CSU, respectively, from 2001 to 2002. Furthermore, L. pneumophila serogroup 3 with an identical PFGE profile B was identified in 20 environmental strains from all wards, L. pneumophila serogroup 3 with PFGE profile C was identified in a single environmental strain from the CR-ICU, and non-pneumophila Legionella with identical PFGE profile D was identified in five environmental strains from the adult CSU, paediatric CSU and NICU. Ultraviolet irradiation was effective in disinfection of the hospital water supplies in the adult and paediatric CSUs contaminated by L. pneumophila clone associated with nosocomial Legionnaires' disease. In conclusion, these data demonstrate that two cases of nosocomial legionellosis were caused by the persistence of a single clone of L. pneumophila serogroup 1/Philadelphia in the hospital environment, and that disinfection by ultraviolet irradiation may represent an effective measure to prevent nosocomial Legionnaires' disease. © 2005 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved
Protozooplankton characterization of two contrasting sites in a tropical coastal ecosystem (Guanabara Bay, RJ)
On the vertical distribution of the chlorophyll <i>a</i> concentration in the Mediterranean Sea: a basin-scale and seasonal approach
The distribution of the chlorophyll a concentration ([Chl a]) in the
Mediterranean Sea, mainly obtained from satellite surface observations or
from scattered in situ experiments, is updated by analyzing a database of
fluorescence profiles converted into [Chl a]. The database, which includes
6790 fluorescence profiles from various origins, was processed with a
specific quality control procedure. To ensure homogeneity between the
different data sources, 65 % of fluorescence profiles have been
intercalibrated on the basis of their concomitant satellite [Chl a]
estimation. The climatological pattern of [Chl a] vertical profiles in four
key sites of the Mediterranean Sea has been analyzed. Climatological results
confirm previous findings over the range of existing [Chl a] values and
throughout the principal Mediterranean trophic regimes. They also provide new
insights into the seasonal variability in the shape of the vertical [Chl a]
profile, inaccessible through remote-sensing observations. An analysis based on
the recognition of the general shape of the fluorescence profile was also
performed. Although the shape of [Chl a] vertical distribution
characterized by a deep chlorophyll maximum (DCM) is ubiquitous during
summer, different forms are observed during winter, thus suggesting that
factors affecting the vertical distribution of the biomass are complex and
highly variable. The [Chl a] spatial distribution in the Mediterranean Sea
mimics, on smaller scales, what is observed in the global ocean. As already
evidenced by analyzing satellite surface observations, midlatitude- and
subtropical-like phytoplankton dynamics coexist in the Mediterranean Sea.
Moreover, the Mediterranean DCM variability appears to be characterized by
patterns already observed on the global scale
The colour of the Mediterranean Sea: Global versus regional bio-optical algorithms evaluation and implication for satellite chlorophyll estimates
International audienc
AN UPDATED ESTIMATE OF PRIMARY PRODUCTION IN THE MEDITERRANEAN SEA FROM REMOTE SENSING DATA.
Legionella contamination in hot water of Italian hotels
A cross-sectional multicenter survey of Italian hotels was conducted to investigate Legionella spp. contamination of hot water. Chemical parameters (hardness, free chlorine concentration, and trace element concentrations), water systems, and building characteristics were evaluated to study risk factors for colonization. The hot water systems of Italian hotels were strongly colonized by Legionella; 75% of the buildings examined and 60% of the water samples were contaminated, mainly at levels of >= 10(3) CFU liter(-1), and Legionella pneumophila was the most frequently isolated species (87%). L. pneumophila serogroup I was isolated from 45.8% of the contaminated sites and from 32.5% of the hotels examined. When a multivariate logistic model was used, only hotel age was associated with contamination, but the risk factors differed depending on the contaminating species and serogroup. Soft water with higher chlorine levels and higher temperatures were associated with L. pneumophila serogroup 1 colonization, whereas the opposite was observed for serogroups 2 to 14. In conclusion, Italian hotels, particularly those located in old buildings, represent a major source of risk for Legionnaires' disease due to the high frequency of Legionella contamination, high germ concentration, and major L. pneumophila serogroup 1 colonization. The possible role of chlorine in favoring the survival of Legionella species is discussed
Colloquium on diatomcopepod interactions
From 3 to 6 November 2002, a colloquium was convened at the Benthos Laboratory of the Stazione Zoologica Anton Dohrn on Ischia, Italy, with the goal of evaluating the present status of the effects of diatoms on their main consumers, planktonic copepods, and to develop future research strategies to enhance our understanding of such interactions. These included (1) toxic effects of diatom metabolites on copepods, particularly reproduction, and (2) nutritional effects of diatoms on juvenile to adult copepods. Key issues involved in the impact of diatoms on the dynamics of natural plankton communities in situ were also addressed. During the plenary session, the most recent advances on this topic were presented. The plenary session was followed by 3 working groups on (1) production of aldehydes by phytoplankton, (2) toxic and nutritional effects of diatoms on zooplankton, and (3) the chemistry of diatom defense, as well as of their nutritional quality. These working groups focused on suggesting future research needs for the different topics. As a result, several recommendations were outlined, including experimental studies. It became evident that interdisciplinary efforts are needed, involving chemists, oceanographers and experimentalists, since many of the biological observations under controlled conditions and in situ require an integrated approach, including chemical causation. Extensive field observations based on common protocols are also recommended for investigation of the intrinsic variability of such effects and their environmental controls. Laboratory experiments are seen to be essential for the full understanding of environmentally occurring processes