SpxA1 and SpxA2 act coordinately to fine-tune stress responses and virulence in Streptococcus pyogenes

SpxA is a unique transcriptional regulator highly conserved among members of the phylum Firmicutes that binds RNA polymerase and can act as an antiactivator. Why some Firmicutes members have two highly similar SpxA paralogs is not understood. Here, we show that the SpxA paralogs of the pathogen Streptococcus pyogenes, SpxA1 and SpxA2, act coordinately to regulate virulence by fine-tuning toxin expression and stress resistance. Construction and analysis of mutants revealed that SpxA1− mutants were defective for growth under aerobic conditions, while SpxA2− mutants had severely attenuated responses to multiple stresses, including thermal and oxidative stresses. SpxA1− mutants had enhanced resistance to the cationic antimicrobial molecule polymyxin B, while SpxA2− mutants were more sensitive. In a murine model of soft tissue infection, a SpxA1− mutant was highly attenuated. In contrast, the highly stress-sensitive SpxA2− mutant was hypervirulent, exhibiting more extensive tissue damage and a greater bacterial burden than the wild-type strain. SpxA1− attenuation was associated with reduced expression of several toxins, including the SpeB cysteine protease. In contrast, SpxA2− hypervirulence correlated with toxin overexpression and could be suppressed to wild-type levels by deletion of speB. These data show that SpxA1 and SpxA2 have opposing roles in virulence and stress resistance, suggesting that they act coordinately to fine-tune toxin expression in response to stress. SpxA2− hypervirulence also shows that stress resistance is not always essential for S. pyogenes pathogenesis in soft tissue

Heavily obscured AGN in the local Universe

We present here a new powerful diagnostic plot to select heavily obscured AGN in the local universe by combining infrared (Spitzer, IRAS) and X-ray (XMM) information. On the basis of this plot, we selected a sample of X-ray obscured sources in the 2XMM catalogue and found seven newly discovered Compton-thick AGN candidates.Comment: 2 pages, 2 figures, To appear in refereed Proceedings of "X-ray Astronomy 2009: Present Status, Multi-Wavelength Approach and Future Perspectives", Bologna, Italy, September 7-11, 2009, AIP, eds. A. Comastri, M. Cappi, and L. Angelin

Quantitative Effects of Iron Chelators on Hydroxyl Radical Production by the Superoxide-Driven Fenton Raction

Iron bound to certain chelators is known to promote the conversion of superoxide radicals (O2) to hydroxyl radicals (HO\u27) by the superoxide-driven Fenton reaction. The production of HO\u27 by various iron chelates was studied using the reaction of dimethyl sulfoxide and HO\u27 to produce methane sulphinic acid. Methane sulphinic acid was quantified by use of a simple colorimetric assay and used to determine the amounts of HO\u27 produced. Superoxide was generated from 200 M hypoxanthine and 0.05 U/ml xanthine oxidase in the presence of 0-100 M iron and 100 M of each chelator. The results of this preliminary investigation illustrate that, at physiological pH, the superoxide-driven Fenton reaction is significantly promoted by iron chelated to EDTA, nitrilotriacetate, and citrate, but is not promoted by the other anions studied

The Palermo Swift-BAT hard X-ray catalogue III. Results after 54 months of sky survey

We present the Second Palermo Swift-BAT hard X-ray catalogue obtained by analysing data acquired in the first 54 months of the Swift mission. Using our software dedicated to the analysis of data from coded mask telescopes, we analysed the BAT survey data in three energy bands (15-30 keV, 15-70 keV, 15-150 keV), obtaining a list of 1256 detections above a significance threshold of 4.8 standard deviations. The identification of the source counterparts is pursued using two strategies: the analysis of field observations of soft X-ray instruments and cross-correlation of our catalogue with source databases.The survey covers 50% of the sky to a 15--150 keV flux limit of 1.0 x 10^-11 erg s^-1 cm^-2 and 9.2 x 10^-12 erg s^-1 cm^-2 for |b| 10 degrees, respectively. The Second Palermo Swift-BAT hard X-ray catalogue includes 1079 (86%) hard X-ray sources with an associated counterpart (26 with a double association and 2 with a triple association) and 177 BAT excesses (14%) that still lack a counterpart. The distribution of the BAT sources among the different object classes consists of 19% Galactic sources, 57% extragalactic sources, and 10% sources with a counterpart at softer energies whose nature has not yet been determined. About half of the BAT associated sources lack a counterpart in the ROSAT catalogues. This suggests that either moderate or strong absorption may be preventing their detection in the ROSAT energy band. The comparison of our BAT catalogue with the Fermi Large Area Telescope First Source Catalogue identifies 59 BAT/Fermi correspondences: 48 blazars, 3 Seyfert galaxies, 1 interacting galaxy, 3 high mass X-ray binaries, and 4 pulsars/supernova remnants. This small number of correspondences indicates that different populations make the sky shine in these two different energy bands

The Crab pulsar light curve in the soft gamma ray range: FIGARO II results

The FIGARO II experiment (a large area, balloon borne, crystal scintillator detector working from 0.15 to 4.3 MeV) observed the Crab pulsar on 1990 Jul. 9 for about seven hours. The study of the pulse profile confirms some structures detected with a low significance during the shorter observation of 1986, and adds new important elements to the picture. In particular, between the two main peaks, two secondary peaks appear centered at phase values 0.1 and 0.3, in the energy range 0.38 to 0.49 MeV; in the same energy range, a spectral feature at 0.44 MeV, interpreted as a redshifted positron annihilation line, was observed during the same balloon flight in the phase interval including the second main peak and the neighboring secondary peak. If the phase interval considered is extended to include also the other secondary peak, the significance of the spectral line appears to increase