Siderose hepatica em tucanos e araçaris /

Orientador : Prof. Dr. Fabiano Montiani-FerreiraDissertaçăo (mestrado) - Universidade Federal do Paraná, Setor de Cięncias Agrárias, Programa de Pós-Graduaçăo em Ciencias Veterinárias. Defesa: Curitiba, 2008Inclui bibliografi

Intoxicação por metal pesado em periquito (Brotogeris Chiriri): relato de caso / Heavy metal poisoning in periquito (Brotogeris Chiriri): case report

Este artigo refere-se a um relato de caso de intoxicação de psitacídeo de vida livre pela ingestão de metal pesado não identificado, seu tratamento e evolução clínica. Um periquito-de-encontro-amarelo (Brotogeris chiriri), ave da ordem Psittaciformes, família Psittacidae, originária de vida livre, foi admitido no hospital veterinário do Refúgio Biológico Bela Vista, com quadro de incoordenação motora ao caminhar, fraqueza muscular, letargia, incapacidade de voo, paresia de membro pélvico direito e inclinação lateral da cabeça. O diagnóstico foi feito com base em exame radiológico, que indicou a presença de material radiodenso no ventrículo. Seu tratamento consistiu na aplicação de Psílio, via sonda oral inserida no inglúvio visando realizar a expulsão mecânica do objeto de densidade metálica e como agente quelante, foi aplicado por via intramuscular o DTPA (Ditripentat-Heyl®). Após alguns dias de tratamento houve melhora evidenciada por ganho de peso, aonde o animal passou a se alimentar por conta própria, retornou à postura e ao estado de consciência normais e voltou a voar.  Conclui-se que o tratamento realizado obteve um resultado satisfatório, comprovando a eficácia na utilização do psyllium e do quelante DTPA (Ditripentat-Heyl®) para o tratamento de intoxicação por metal pesado. O paciente recebeu alta e foi solto em seu ambiente natural 36 dias após sua admissão no hospital veterinário. 

Detection of Plasmodium sp. in capybara

In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the two capybara sequences. When compared with GenBank sequences, a 55% similarity was obtained to Toxoplasma gondii and a higher similarity (73– 77.2%) was found to ssrRNAs from Plasmodium species that infect reptile, avian, rodents, and human beings. The most similar Plasmodium sequence was from Plasmodium mexicanum that infects lizards of North America, where around 78% identity was found. This work is the first report of Plasmodium in capybaras, and due to the low similarity with other Plasmodium species, we suggest it is a new species, which, in the future could be denominated ''Plasmodium hydrochaeri''

Siderose hepatica em tucanos e araçaris /

Orientador : Prof. Dr. Fabiano Montiani-FerreiraDissertaçăo (mestrado) - Universidade Federal do Paraná, Setor de Cięncias Agrárias, Programa de Pós-Graduaçăo em Ciencias Veterinárias. Defesa: Curitiba, 2008Inclui bibliografi

Occurrence of sarcocystis falcatula in captive psittacine birds in Brazil

Thirty-eight captive psittacine birds housed in a bird park in Foz do Iguaçu, Parana, Brazil, died within a 15-month period as a result of infection with Sarcocystis falcatula. Although fatalities affected 16 species of psittacine birds, mortality was highest in Old World species, which were most susceptible to the pulmonary form of sarcocystosis. Along with the pathologic findings of this disease outbreak, a review of the pathophysiology of sarcosporidiosis is presentedFAPES

ARTs in wild felid conservation programmes in Poland and in the world

With the exception of the domestic cat, all felid species (Felidae) are currently threatened with extinction in their natural habitat. To develop effective and optimal wild cat conservation programmes with assisted reproductive technology (ART) it is necessary to combine advances from different disciplines of science, starting from the biology of the species, through research into the population and habitat, assisted reproductive technologies, establishment of gene banks, developing bioinformatic systems, and ending with biodiversity and endangered species management. In the last few years knowledge of felid reproduction has expanded considerably thanks to comparative studies utilising the domestic cat as a research model for endangered wild cats. Basic reproductive techniques utilised in both domestic cat breeding and rescuing wild felid populations that are threatened with extinction include semen collection and cryopreservation, artificial insemination, oocyte collection, in vitro maturation, in vitro fertilisation, somatic cloning, and embryo transfer. The main directions in which assisted reproductive technologies are being developed in wild cat conservation implementations and the contribution of Polish research centres in advancing these methods are presented

First molecular screening of Plasmodium species in ungulates from Southern Brazil

Abstract Objective Despite malaria epidemiology has been extensively studied in primates, few studies were conducted in ungulates. After half a century without descriptions of Plasmodium spp. in deer since its first identification, recent research has rediscovered Plasmodium on ungulates in Africa, Asia, North America and South America, including Central Brazil. Here, a captive herd was evaluated in southern Brazil using light microscopy and PCR. DNA samples were tested for fragment amplification of two Plasmodium spp. genes: mitochondrial cytochrome b and small subunit ribosomal RNA. Results All analyses were negative. However, the tests were performed on samples that were collected at a single time point, and parasitemia may fluctuate over the parasite’s life cycle. Thus, the possibility of occult infection cannot be ruled out. Despite the negative results of all of the methods applied, it cannot be categorically stated that these animals are free from Plasmodium sp. infection. Further monitoring and/or multiple sequential sampling may improve the success rate of detecting parasites. Moreover, although this survey of Plasmodium represents the first molecular study on ungulate malaria parasites from Southern Brazil, further analysis of samples from different ungulate species is important for characterizing the epidemiology of Plasmodium of these mammals in this region