Novo instrumento automático para medida de pressão intracelular em vegetais.

Neste trabalho será mostrado como o modelo do instrumento é utilizado de forma a auxiliar no projeto de uma sonda de pressão automática, além dos resultados práticos obtidos em experimentos de bancada para caracterização da sonda. A versão da sonda de pressão usada para o trabalho foi a de compensação térmica e a automação é baseada na teoria de Sistemas de Controle.bitstream/CNPDIA/8123/1/CiT17_2002.pd

Modelo Validado de Sonda de Pressão para Medida de Relações de Água em Plantas.

bitstream/CNPDIA/10482/1/DOC16_2005.pd

Abordagem de sistemas de controle para desenvolvimento de instrumento de medida de pressão intracelular.

bitstream/CNPDIA/10190/1/PA27_98.pd

Modelagem e automação de uma sonda termoelástica para medidas de parâmetros relativos a fisiologia vegetal.

bitstream/CNPDIA-2009-09/11849/1/BPD14_2006.pd

Automação de um sistema de medida de turgescência em plantas.

bitstream/CNPDIA/9856/1/PA15_97.pd

Nonoperative Management Of Splenic Injury Grade Iv Is Safe Using Rigid Protocol [tratamento Não Operatório De Lesão Esplênica Grau Iv é Seguro Usando-se Rígido Protocolo]

Objective: To demonstrate the protocol and experience of our service in the nonoperative management (NOM) of grade IV blunt splenic injuries. Methods: This is a retrospective study based on trauma registry of a university hospital between 1990-2010. Charts of all patients with splenic injury were reviewed and patients with grade IV lesions treated nonoperatively were included in the study. Results: ninety-four patients with grade IV blunt splenic injury were admitted during this period. Twenty-six (27.6%) met the inclusion criteria for NOM. The average systolic blood pressure on admission was 113.07 ± 22.22 mmHg, RTS 7.66 ± 0.49 and ISS 18.34 ± 3.90. Ten patients (38.5%) required blood transfusion, with a mean of 1.92 ± 1.77 packed red cells per patient. Associated abdominal injuries were present in two patients (7.7%). NOM failed in two patients (7.7%), operated on due to worsening of abdominal pain and hypovolemic shock. No patient developed complications related to the spleen and there were no deaths in this series. Average length of hospital stay was 7.12 ± 1.98 days. Conclusion: Nonoperative treatment of grade IV splenic injuries in blunt abdominal trauma is safe when a rigid protocol is followed.404323328Raza, M., Abbas, Y., Devi, V., Prasad, K.V., Rizk, K.N., Nair, P.P., Non operative management of abdominal trauma-a 10 years review (2013) World J Emerg Surg., 8 (1), p. 14. , [online] Acessado em 05/04/2013Moore, E.E., Cogbill, T.H., Jurkovich, G.J., Shackford, S.R., Malangoni, M.A., Champion, H.R., Organ injury scaling: Spleen and liver (1994 revision) (1995) J Trauma, 38, p. 323Jeremitsky, E., Smith, R.S., Ong, A.W., Starting the clock: Defining nonoperative management of blunt splenic injury by time (2013) Am J Surg., 205 (3), pp. 298-301Lippert, S.J., Hartin Jr., C.W., Ozgediz, P.L., Glick, P.L., Caty, M.G., Flynn, W.J., Splenic conservation: Variation between pediatric and adut trauma centers (2013) J Surg Res., 182 (1), pp. 17-20Stassen, N.A., Bhullar, I., Cheng, J.D., Crandall, M.L., Friese, R.S., Guillamondegui, O.D., Selective nonoperative management of blunt splenic injury: An Eastern Association for the Surgery of Trauma practice management guideline (2012) J Trauma Acute Care Surg., 73 (5 SUPPL. 4), pp. S294-S300Starling, S.V., Rodrigues, J.M.S., Reis, M.C.W., Trauma contuso do baço: Quando operar? (2011) Atualidades em Clínica Cirúrgica-Intergastro e Trauma, pp. 29-51. , In: Fraga GP, Sevá-Pereira G, Lopes LR, Editora Atheneu, São Paulo, 2011Carvalho, F.H., Romeiro, P.C., Collaço, I.A., Baretta, G.A., Freitas, A.C., Matias, J.E., Prognostics factors related to non surgical treatment failure of splenic injuries in the abdominal blunt trauma (2009) Rev Col Bras Cir., 36 (2), pp. 123-130Olthof, D.C., Joosse, P., Van Der Vlies, C.H., De Haan, R.J., Goslings, J.C., Prognostic factors for failure of nonoperative management in adults with blunt splenic injury: A systematic review (2013) J Trauma Acute Care Surg., 74 (2), pp. 546-557Bhullar, I.S., Frykberg, E.R., Siragusa, D., Chesire, D., Paul, J., Tepas III, J.J., Age does not affect outcomes of nonperative management of blunt splenic trauma (2012) J Am Coll Surg., 214 (6), pp. 958-964Galvan, D.A., Peitzman, A.B., Failure of nonoperative management of abdominal solid organ injuries (2006) Curr Opin Crit Care., 12 (6), pp. 590-594Mantovani, M., Mauro, J.F., Fraga, G.P., Meirelles, G.V., Trauma abdominal fechado: Tratamento não-operatório das lesões esplênicas (2002) Rev Para Med., 16 (2), pp. 46-51Zarzaur, B.L., Kozar, R.A., Fabian, T.C., Coimbra, R., A survey of American Association for the Surgery of Trauma member practices in the management of blunt splenic injury (2011) J Trauma., 70 (5), pp. 1026-1031Bhullar, I.S., Frykberg, E.R., Tepas III, J.J., Siragusa, D., Loper, T., Kerwin, A.J., At first blush: Absence of computed tomography contrast extravasation in grade IV or V adult blunt splenic trauma should not preclude angioembolization (2013) J Trauma Acute Care Surg., 74 (1), pp. 105-111Meguid, A.A., Bair, H.A., Howells, G.A., Bendick, P.J., Kerr, H.H., Villalba, M.R., Prospective evaluation of criteria for the nonoperative management of blunt splenic trauma (2003) Am Surg., 69, pp. 238-243Peitzman, A.B., Heil, B., Rivera, L., Federle, M.B., Harbrecht, B.G., Clancy, K.D., Blunt splenic injury in adults: Multi-institutional study of the Eastern Association for the surgery of trauma (2000) J Trauma., 49, pp. 177-189Pearl, R.H., Wesson, D.E., Spence, L.J., Filler, R.M., Ein, S.H., Shandling, B., Splenic injury: A 5-year update with improved results and changing criteria for conservative management (1989) J Pediatr Surg., 24, pp. 428-431Albrecht, R.M., Schermer, C.R., Morris, A., Nonoperative management of blunt splenic injuries: Factors influencing success in age > 55 years (2002) Am Surg., 68, pp. 227-231Cocanour, C.S., Moore, F.A., Ware, D.N., Marvin, R.G., Duke, J.H., Age should not be a consideration for nonoperative management of blunt splenic injury (2000) J Trauma., 48, pp. 606-612Tsugawa, K., Koyanagi, N., Hashizume, M., Ayukawa, K., Wada, H., Tomikawai, M., New insight for management of blunt splenic trauma: Significant differences between young and elderly (2002) Hepato-gastroenterology., 49, pp. 1144-1149Krause, K.R., Howells, G.A., Bair, H.A., Glover, J.L., Madrazo, B.L., Wasvary, H.J., Nonoperative management of blunt splenic injury in adults 55 years and older: A twenty-year experience (2000) Am Surg., 66, pp. 636-640Harbrecht, B.G., Peitzman, A.B., Rivera, L., Heil, B., Croce, M., Morris Jr., J.A., Contribution of age and gender to outcome of blunt splenic injury in adults: Multicenter study of the eastern association for the surgery of trauma (2001) J Trauma., 51, pp. 887-895Willmann, J.K., Roos, J.E., Platz, A., Pfammatter, T., Hilfiker, P.R., Marincek, B., Multidetector CT: Detection of active hemorrhage in patients with blunt abdominal trauma (2002) AJR., 179 (2), pp. 437-444Malangoni, M.A., Cué, J.I., Fallat, M.E., Willing, S.J., Richardson, J.D., Evaluation of splenic injury by computed tomography and its impact on treatment (1990) Ann Surg., 211, pp. 592-599Mirvis, S.E., Whitley, N.O., Gens, D.R., Blunt splenic trauma in adults: CTbased classification and correlation with prognosis and treatment (1989) Radiology., 171, pp. 33-39Skattum, J., Titze, T.L., Dormagen, J.B., Aaberge, I.S., Bechensteen, A.G., Gaarder, P.I., Preserved splenic function after angioembolisation of high grade injury (2012) Injury., 43, pp. 62-66Pachter, H.L., Guth, A.A., Hofstetter, S.R., Spencer, F.C., Changing patterns in the management of splenic trauma: The impact of nonoperative management (1998) Ann Surg., 227, pp. 708-719Powell, M., Courcoulas, A., Gardner, M., Lynch, J., Harbrecht, B.G., Udekwu, A.O., Management of blunt splenic trauma: Significant differences between adults and children (1997) Surgery, 122, pp. 654-660Hunt, J.P., Lentz, C.W., Cairns, B.A., Ramadan, F.M., Smith, D.L., Rutledge, R., Management and outcome of splenic injury: The results of a five-year statewide population-based study (1996) Am. Surg., 62, pp. 911-917Velmahos, G.C., Zacharias, N., Emhoff, T.A., Feeney, J.M., Hurst, J.M., Crookes, B.A., Management of the most severely injured spleen: A multicenter study of the Research Consortium of New England Centers for Trauma (ReCONECT) (2010) Arch Surg., 145 (5), pp. 456-460Branco, B.C., Tang, A.L., Rhee, P., Fraga, G.P., Nascimento, B., Rizoli, S., O'Keeffe, T., Selective Nonoperative Management of High Grade Splenic Trauma (2013) Rev Col Bras Cir, 40 (3), pp. 246-25

Sistema eletrônico para leitura e aquisição de dados de sensores descartáveis, aplicados à detecção de voláteis, desenvolvidos com filmes ultrafinos de poli-anilinas condutoras.

bitstream/CNPDIA-2009-09/11040/1/CT90_2007.pd

Tomógrafo de resolução micrométrica para estudos de solos.

bitstream/CNPDIA/9917/1/BP04_97.pd

Mutational profile of TP53 in esophageal squamous cell carcinoma associated with chagasic megaesophagus

Chaga's disease is an important communicable neglected disease that is gaining wider attention due to its increasing incidence worldwide. Achalasia due to chagasic megaesophagus (CM), a complication of this disease, is a known-yet, poorly understood-etiological factor for esophageal squamous cell carcinoma (ESCC) development. In this study, we aimed to perform the analysis of TP53 mutations in a series of Brazilian patients with ESCC that developed in the context CM (ESCC/CM), and to compare with the TP53 mutation profile of patients with benign CM and patients with nonchagasic ESCC. Additionally, we intended to correlate the TP53 mutation results with patient's clinical pathological features. By polymerase chain reaction (PCR) followed by direct sequencing of the hotspot regions of TP53 (exon 5 to 8), we found that TP53 mutations were present in 40.6% (13/32) of the ESCC/CM group, 45% (18/40) of the nonchagasic ESCC group, and in only 3% (1/33) of the benign CM group. Missense mutations were the most common in the three groups, yet, the type and mutated exon mutation varied significantly among the groups. Clinically, the groups exhibited distinct features, with both cancer groups (ESCC and ESCC/CM) been significantly associated higher consumption of alcohol and tobacco, older age, worse Karnofsky performance status, poor outcome than the patients with benign CM. No significant association was found between TP53 mutation profile and clinical-pathological features in any of the three groups. We describe first the time the analysis of TP53 mutations in ESCC that developed in the context of CM, and the observed high frequency of mutations, suggest that TP53 also plays an important role in the tumorigenic process of this unexplored etiological condition.Supported by Barretos Cancer Hospital internal research funds (PAIP). Rui M Reis is recipient of a CNPq Produtividade em Pesquisa grantinfo:eu-repo/semantics/publishedVersio

Absence of TERT promoter mutations in colorectal precursor lesions and cancer

Hotspot mutations (c.-124bp G > A and c.-146bp G > A) in the promoter region of the TERT gene have been recently described in several types of solid tumors, including glioma, bladder, thyroid, liver and skin neoplasms. However, knowledge with respect to colorectal precursor lesions and cancer is scarce. In the present study we aimed to determine the frequency of hotspot TERT promoter mutations in 145 Brazilian patients, including 103 subjects with precursor lesions and 42 with colorectal carcinomas, and we associated the presence of such mutations with the patients clinical-pathological features. The mutation analysis was conclusive in 123 cases, and none of the precursor and colorectal carcinoma cases showed TERT promoter mutations. We conclude that TERT mutations are not a driving factor in colorectal carcinogenesis.This study was financially partially supported by Barretos Cancer Hospital Internal Research Funds (PAIP) to participating authorsinfo:eu-repo/semantics/publishedVersio