Interaction Between The MEC1-Dependent DNA Synthesis Checkpoint And G1 Cyclin Function In Saccharomyces Cerevisiae

The completion of DNA synthesis in yeast is monitored by a checkpoint that requires MEC1 and RAD53. Here we show that deletion of the Saccharomyces cerevisiae G1 cyclins CLN1 and CLN2 suppressed the essential requirement for MEC1 function. Wild-type levels of CLN1 and CLN2, or overexpression of CLN1, CLN2, or CLB5, but not CLN3, killed mec1 strains. We identified RNR1, which encodes a subunit of ribonucleotide reductase, as a high-copy suppressor of the lethality of mec1 GAL1-CLN1. Northern analysis demonstrated that RNR1 expression is reduced by CLN1 or CLN2 overexpression. Because limiting RNR1 expression would be expected to decrease dNTP pools, CLN1 and CLN2 may cause lethality in mec1 strains by causing initiation of DNA replication with inadequate dNTPs. In contrast to mec1 mutants, MEC1 strains with low dNTPs would be able to delay S phase and thereby remain viable. We propose that the essential function for MEC1 may be the same as its checkpoint function during hydroxyurea treatment, namely, to slow S phase when nucleotides are limiting. In a cln1 cln2 background, a prolonged period of expression of genes turned on at the G1-S border, such as RNR1, has been observed. Thus deletion of CLN1 and CLN2 could function similarly to overexpression of RNR1 in suppressing mec1 lethality

Rayleigh-Benard Convection with a Radial Ramp in Plate Separation

Pattern formation in Rayleigh-Benard convection in a large-aspect-ratio cylinder with a radial ramp in the plate separation is studied analytically and numerically by performing numerical simulations of the Boussinesq equations. A horizontal mean flow and a vertical large scale counterflow are quantified and used to understand the pattern wavenumber. Our results suggest that the mean flow, generated by amplitude gradients, plays an important role in the roll compression observed as the control parameter is increased. Near threshold the mean flow has a quadrupole dependence with a single vortex in each quadrant while away from threshold the mean flow exhibits an octupole dependence with a counter-rotating pair of vortices in each quadrant. This is confirmed analytically using the amplitude equation and Cross-Newell mean flow equation. By performing numerical experiments the large scale counterflow is also found to aid in the roll compression away from threshold but to a much lesser degree. Our results yield an understanding of the pattern wavenumbers observed in experiment away from threshold and suggest that near threshold the mean flow and large scale counterflow are not responsible for the observed shift to smaller than critical wavenumbers.Comment: 10 pages, 13 figure

Extensive chaos in Rayleigh-Bénard convection

Using large-scale numerical calculations we explore spatiotemporal chaos in Rayleigh-Bénard convection for experimentally relevant conditions. We calculate the spectrum of Lyapunov exponents and the Lyapunov dimension describing the chaotic dynamics of the convective fluid layer at constant thermal driving over a range of finite system sizes. Our results reveal that the dynamics of fluid convection is truly chaotic for experimental conditions as illustrated by a positive leading-order Lyapunov exponent. We also find the chaos to be extensive over the range of finite-sized systems investigated as indicated by a linear scaling between the Lyapunov dimension of the chaotic attractor and the system size

Traveling waves in rotating Rayleigh-Bénard convection: Analysis of modes and mean flow

Numerical simulations of the Boussinesq equations with rotation for realistic no-slip boundary conditions and a finite annular domain are presented. These simulations reproduce traveling waves observed experimentally. Traveling waves are studied near threshhold by using the complex Ginzburg-Landau equation (CGLE): a mode analysis enables the CGLE coefficients to be determined. The CGLE coefficients are compared with previous experimental and theoretical results. Mean flows are also computed and found to be more significant as the Prandtl number decreases (from sigma=6.4 to sigma=1). In addition, the mean flow around the outer radius of the annulus appears to be correlated with the mean flow around the inner radius

Rayleigh-Benard Convection in Large-Aspect-Ratio Domains

The coarsening and wavenumber selection of striped states growing from random initial conditions are studied in a non-relaxational, spatially extended, and far-from-equilibrium system by performing large-scale numerical simulations of Rayleigh-B\'{e}nard convection in a large-aspect-ratio cylindrical domain with experimentally realistic boundaries. We find evidence that various measures of the coarsening dynamics scale in time with different power-law exponents, indicating that multiple length scales are required in describing the time dependent pattern evolution. The translational correlation length scales with time as $t^{0.12}$, the orientational correlation length scales as $t^{0.54}$, and the density of defects scale as $t^{-0.45}$. The final pattern evolves toward the wavenumber where isolated dislocations become motionless, suggesting a possible wavenumber selection mechanism for large-aspect-ratio convection.Comment: 5 pages, 6 figure

NASA rotor system research aircraft flight-test data report: Helicopter and compound configuration

The flight test activities of the Rotor System Research Aircraft (RSRA), NASA 740, from June 30, 1981 to August 5, 1982 are reported. Tests were conducted in both the helicopter and compound configurations. Compound tests reconfirmed the Sikorsky flight envelope except that main rotor blade bending loads reached endurance at a speed about 10 knots lower than previously. Wing incidence changes were made from 0 to 10 deg

Power-Law Behavior of Power Spectra in Low Prandtl Number Rayleigh-Benard Convection

The origin of the power-law decay measured in the power spectra of low Prandtl number Rayleigh-Benard convection near the onset of chaos is addressed using long time numerical simulations of the three-dimensional Boussinesq equations in cylindrical domains. The power-law is found to arise from quasi-discontinuous changes in the slope of the time series of the heat transport associated with the nucleation of dislocation pairs and roll pinch-off events. For larger frequencies, the power spectra decay exponentially as expected for time continuous deterministic dynamics.Comment: (10 pages, 6 figures

Multilevel mesh partitioning for optimising domain shape

Multilevel algorithms are a successful class of optimisation techniques which address the mesh partitioning problem for mapping meshes onto parallel computers. They usually combine a graph contraction algorithm together with a local optimisation method which refines the partition at each graph level. To date these algorithms have been used almost exclusively to minimise the cut-edge weight in the graph with the aim of minimising the parallel communication overhead. However it has been shown that for certain classes of problem, the convergence of the underlying solution algorithm is strongly influenced by the shape or aspect ratio of the subdomains. In this paper therefore, we modify the multilevel algorithms in order to optimise a cost function based on aspect ratio. Several variants of the algorithms are tested and shown to provide excellent results