2,594 research outputs found
A Millikelvin Scanned Probe for Measurement of Nanostructures
We demonstrate a scanning force microscope, based upon a quartz tuning fork,
that operates below 100 mK and in magnetic fields up to 6 T. The microscope has
a conducting tip for electrical probing of nanostructures of interest, and it
incorporates a low noise cryogenic amplifier to measure both the vibrations of
the tuning fork and the electrical signals from the nanostructures. At
millikelvin temperatures the imaging resolution is below 1 um in a 22 um x 22
um range, and a coarse motion provides translations of a few mm. This scanned
probe is useful for high bandwidth measurement of many high impedance
nanostructures on a single sample. We show data locating an SET within an array
and measure its coulomb blockade with a sensitivity of 2.6 x 10^-5 e/Hz^1/2.Comment: 5 pages, 5 figures, submitted to RS
Dynamically-Coupled Oscillators -- Cooperative Behavior via Dynamical Interaction --
We propose a theoretical framework to study the cooperative behavior of
dynamically coupled oscillators (DCOs) that possess dynamical interactions.
Then, to understand synchronization phenomena in networks of interneurons which
possess inhibitory interactions, we propose a DCO model with dynamics of
interactions that tend to cause 180-degree phase lags. Employing an approach
developed here, we demonstrate that although our model displays synchronization
at high frequencies, it does not exhibit synchronization at low frequencies
because this dynamical interaction does not cause a phase lag sufficiently
large to cancel the effect of the inhibition. We interpret the disappearance of
synchronization in our model with decreasing frequency as describing the
breakdown of synchronization in the interneuron network of the CA1 area below
the critical frequency of 20 Hz.Comment: 10 pages, 3 figure
Thrombosis of a Superior Mesenteric Vein Aneurysm: Transarterial Thrombolysis and Transhepatic Aspiration Thrombectomy
We report the case of a 31-year-old woman presenting with abdominal pain due to acute thrombosis of a superior and inferior mesenteric vein aneurysm, which was treated by a combination of arterial thrombolysis and transhepatic thrombus aspiration. At the last follow-up CT, 21 months following this procedure, there was no evidence of rethrombosis, and the patient continues to do well under oral anticoagulation. The literature regarding these uncommon mesenteric vein aneurysms without portal vein involvement, as well as their treatment options, is reviewe
Groups of Galaxies in the Two Micron All-Sky Redshift Survey
We present the results of applying a percolation algorithm to the initial
release of the Two Micron All-Sky Survey Extended Source Catalog, using
subsequently measured redshifts for almost all of the galaxies with K < 11.25
mag. This group catalog is based on the first near-IR all-sky flux-limited
survey that is complete to |b| = 5 deg. We explore the dependence of the
clustering on the length and velocity scales involved. The paper describes a
group catalog, complete to a limiting redshift of 10,000 km/s, created by
maximizing the number of groups containing 3 or more members. A second catalog
is also presented, created by requiring a minimum density contrast of 80 to
identify groups. We identify known nearby clusters in the catalogs and contrast
the groups identified in the two catalogs. We examine and compare the
properties of the determined groups and verify that the results are consistent
with the UZC-SSRS2 and northern CfA redshift survey group catalogs. The all-sky
nature of the catalog will allow the development of a flow-field model based on
the density field inferred from the estimated cluster masses.Comment: Accepted for publication in ApJ (29 pages including 13 figures). A
version with high-resolution figures is available at
http://www.cfa.harvard.edu/~acrook/preprints
The role of interspecific variability and herbicide pre-adaptation in the cinmethylin response of Alopecurus myosuroides
BACKGROUND: Cinmethylin is an inhibitor of plant fatty acid biosynthesis, with in-plant activity caused by its binding to fatty acid thioesterases (FAT). The recent registration of cinmethylin for pre-emergence herbicidal use in the UK represents a new mode of action (MOA) for control of the grassweed blackgrass (Alopecurus myosuroides). To date there is little published information on the extent of blackgrassâ inter-population variability in sensitivity to cinmethylin, nor on any potential effect of existing non-target-site resistance (NTSR) mechanisms on cinmethylin efficacy.
RESULTS: Here we present a study of variability in cinmethylin sensitivity amongst 97 UK blackgrass populations. We demonstrate that under controlled conditions, a UK field-rate dose of 500 g ha-1 provides effective control of the tested populations. Nevertheless, we reveal significant inter-population variability at doses below this rate, with populations previously characterised as strongly NTSR displaying the lowest sensitivity to cinmethylin. Assessment of paired resistant âRâ and sensitive âSâ lines from standardised genetic backgrounds confirms that selection for NTSR to the acetyl-CoA-carboxylase inhibitor fenoxaprop, and the microtubule assembly inhibitor pendimethalin, simultaneously results in reduced sensitivity to cinmethylin at doses below 500 g ha-1. Whilst we find no resistance to the field-rate dose, we reveal that cinmethylin sensitivity can be further reduced through experimental selection with cinmethylin.
CONCLUSION: Cinmethylin therefore represents a much-needed further MOA for blackgrass control, but needs to be carefully managed within a resistance monitoring and integrated weed management (IWM) framework to maximise the effective longevity of this compound
External and internal noise surveys of London primary schools
Internal and external noise surveys have been carried out around schools in London, UK, to provide information on typical levels and sources to which children are exposed while at school. Noise levels were measured outside 142 schools, in areas away from flightpaths into major airports. 86% of the schools surveyed were exposed to noise from road traffic, the average external noise level outside a school being 57 dB LAeq. Detailed internal noise surveys have been carried out in 140 classrooms in 16 schools, together with classroom observations. It was found that noise levels inside classrooms depend upon the activities in which the children are engaged, with a difference of 20 dB LAeq between the 'quietest' and 'noisiest' activities. The average background noise level in classrooms exceeds the level recommended in current standards. The number of children in the classroom was found to affect noise levels. External noise influenced internal noise levels only when children were engaged in the quietest classroom activities. The effects of the age of the school buildings and types of window upon internal noise were examined but results were inconclusive
Optimizing DNA Extraction Methods for Nanopore Sequencing of Neisseria gonorrhoeae Directly from Urine Samples
Empirical gonorrhea treatment at initial diagnosis reduces onward transmission. However, increasing resistance to multiple antibiotics may necessitate waiting for culture-based diagnostics to select an effective treatment. There is a need for same-day culture-free diagnostics that identify infection and detect antimicrobial resistance. We investigated if Nanopore sequencing can detect sufficient Neisseria gonorrhoeae DNA to reconstruct whole genomes directly from urine samples. We used N. gonorrhoeae-spiked urine samples and samples from gonorrhea infections to determine optimal DNA extraction methods that maximize the amount of N. gonorrhoeae DNA sequenced while minimizing contaminating host DNA. In simulated infections, the Qiagen UCP pathogen mini kit provided the highest ratio of N. gonorrhoeae to human DNA and the most consistent results. Depletion of human DNA with saponin increased N. gonorrhoeae yields in simulated infections but decreased yields in clinical samples. In 10 urine samples from men with symptomatic urethral gonorrhea, â„92.8% coverage of an N. gonorrhoeae reference genome was achieved in all samples, with â„93.8% coverage breath at â„10-fold depth in 7 (70%) samples. In simulated infections, if â„104 CFU/ml of N. gonorrhoeae was present, sequencing of the large majority of the genome was frequently achieved. N. gonorrhoeae could also be detected from urine in cobas PCR medium tubes and from urethral swabs and in the presence of simulated Chlamydia coinfection. Using Nanopore sequencing of urine samples from men with urethral gonorrhea, sufficient data can be obtained to reconstruct whole genomes in the majority of samples without the need for culture
From multiple perspectives to shared understanding
The aim of this study was to explore how learners operating in a small group reach shared understanding as they work out joint research questions and build a theoretical framework and to identify the resources and tools they used in the process. The learnersâ own interpretations of their group activities and learning were also taken into account. The data, consisting of group discussions and the documents produced by the group, were subjected to a qualitative content analysis. The group members employed a variety of resources and tools to exchange their individual perspectives and achieve shared understanding. Summaries of relevant literature laid a foundation for the groupâs theoretical discussions. Reflective comparisons between their book knowledge and their personal experiences of online interaction and collaboration were frequent, suggesting that such juxtapositions may have enhanced their learning by intertwining the content to be mastered and the activities entailed by this particular content
Preferences for Medical Consultations from Online Providers: Evidence from a Discrete Choice Experiment in the United Kingdom
Background:
In the UK, consultations for prescription medicines are available via private providers such as online pharmacies. However, these providers may have lower thresholds for prescribing certain drugs. This is a particular concern for antibiotics, given the increasing burden of antimicrobial resistance. Public preferences for consultations with online providers are unknown, hence the impact of increased availability of online consultations on antibiotic use and population health is unclear.
Objective:
To conduct a discrete choice experiment survey to understand UK public preferences for seeking online consultations, and the factors that influence these preferences, in the context of having symptoms for which antibiotics may be appropriate.
Methods:
In a survey conducted between July and August 2018, general population respondents completed 16 questions in which they chose a primary care consultation via either their local medical centre or an online provider. Consultations were described in terms of five attributes, including cost and similarity to traditional âface-to-faceâ appointments. Choices were modelled using regression analysis.
Results:
Respondents (n = 734) placed a high value on having a consultation via their local medical centre rather than an online provider, and a low value on consultations by phone or video. However, respondents characterised as âbusy young professionalsâ showed a lower strength of preference for traditional consultations, with a higher concern for convenience.
Conclusion:
Before COVID-19, the UK public had limited appetite for consultations with online providers, or for consultations that were not face-to-face. Nevertheless, prescriptions from online providers should be monitored going forward, particularly for antibiotics, and in key patient groups
- âŠ