Development of an avian tuberculosis vaccine for wildfowl

The Wildfowl and Wetlands Trust maintain a large and comprehensive collection of captive wildfowl at Slimbridge in Gloucestershire, Avian tuberculosis caused by Mycobacterium avium, has been enzootic since 1948 and is now the main cause of death, accounting for over a third of post fledgling mortalities. There is a grave risk of spreading infection to the enormous number of wild birds that use the grounds. The introductory chapters review the disease in both wild and captive wild birds. The particular susceptibility of the rarest duck in the world: the White-winged Wood Duck Cairina scutulata, and the effect of the disease on its captive breeding programme, are discussed. The disease situation at Slimbridge is assessed in an epizootiological study which is carried out on adult post mortem data of the years 1980-1989. This study shows both genetic and environmental factors greatly influencing incidence of the disease. However, it also suggests that there are definite levels of immunity to M.avium in some groups and individuals. The main experiments in the thesis investigate the possibility of immunoprophylaxis in the form of a vaccine as a possible method of controlling the disease. Past attempts at vaccination are reviewed, as is the current knowledge of the wildfowl immune system. Conditions for the immunological tests used to assess immune responses throughout the vaccine studies had to be optimised. These tests included: wildfowl lymphocyte transformation test (LTT) which has been made to work in the presence of antigen for the first time; a method for skin testing, previously not done in wildfowl; and an enzyme linked immunosorbence assay (ELISA). The main study chapters report the use of intradermal BCG and M.vaccae as potential vaccines. Studies with Mallard Anas platyrhynchos platyrhynchos indicate killed M.vaccae administered at one day old as an immunopotentiating agent. Studies with Mandarin ducks Aix galericulata confirm the optimum age for vaccination to be one day old. An optimum dose of this vaccine has been elucidated in studies using Gadwall Anas strepera and Hawaiian Geese or Nenes Branta sandvicensis. No protection was afforded to White-winged Wood Ducks when vaccinated with killed M.vaccae in adult life. A bacteriological study of strains of M.avium isolated from tuberculous birds at post mortem is also reported. Strains were isolated from birds from both Slimbridge and Arundel Wildfowl and Wetlands Trust centres, and also from several wild birds. These strains were subjected to lipid and DNA analysis, and studies were made of their protein patterns using SDS-polyacrylamide gel electrophoresis. The current vaccination programme at Slimbridge and The Wildfowl and Wetlands Trust centre at Llanelli, South Wales, is discussed. Results from tuberculous birds in the vaccine trials indicate the potential for the development of a diagnostic test

Migratory Species and Health: A Review of Migration and Wildlife Disease Dynamics, and the Health of Migratory Species, within the Context of One Health

A desire for good health unites us all. Still in a time of COVID-19 and with the looming threat of future pandemics, we are reminded of the fragility of our collective health. Now more than ever, we understand that human health is inexorably linked to the health of the environment in which we live and the species, be they wild or domestic, on which we depend. As we threaten and change our climate, transform natural landscapes, intensify our agricultureactivities, unsustainably exploit resources, and pollute our air, land and water, the pressures on the environment and on migratory species have never been greater. All of these actions in turn drive the emergence of diseases and increase our fragility. I have lived through a time when diseases of wildlife may have been only of scientific interest to some. Now we see the wider and significant consequences of the emergence of both infectious and non-infectious diseases and the growing threat they pose to the very survival of species.Bringing UNEP into the health Quadripartite to work alongside the World HealthOrganization, UN Food and Agriculture Organization and the World Animal Health Organization is a clear sign that, as a society, if we are to tackle global human ill-health we need to pay significant attention to One Health, taking a holistic approach to disease issues at the global level. Yet within One Health the environment and wildlife health are too often the ‘poor relation.’ There remains too little understanding of disease dynamics and scant consideration of wildlife health when making decisions on food production, trade, land planning, energy production and infrastructure development. Due to the now obvious interconnectivity of health, we know that what is bad for wildlife health, is ultimately bad for us.Too often we overlook the value of wildlife health, only opening our eyes when we feel the negative consequence for human ill-health. For example, when we are confronted with the appalling sights of acute outbreaks of diseases like highly pathogenic avian influenza, or when wildlife diseases spill into livestock or zoonotic infections affect people and present pandemic risks. There is a need to turn the adage of ‘no prizes in prevention’ into applauded actions to maintain the integrity and resilience of ecosystems to stop disease emergence at its source. Developing cost-effective ways to prevent disease emergence that also benefit ecosystems is surely a key challenge for the future.This Report, authored and reviewed by a world leading team of wildlife health specialists, led by the University of Edinburgh, contributes to the growing body of work which highlights the needs for interdisciplinary action to protect the health of us all. Instead of viewing issues through the single anthropocentric lens of human health, it takes the perspective of the wider environment and of the species within, helping to rebalance and improve our thinking aboutOne Health.The Report is a seminal contribution to the work of the Convention on Migratory Species (CMS) on the issue of wildlife health and will guide the work of the CMS Working Group on Migratory Species and Health. It adds to the CMS work on One Health issues such as the poisoning of species, and highly pathogenic avian influenza, which has helped guide Parties and other stakeholders dealing with the negative health consequences of human activities.The Report examines our current understanding of the determinants of health and considers the gains to be made from taking One Health approaches. With a frequent perception of migratory species as vectors of disease it reviews the complexities of their disease dynamics and considers both the benefits and dis-benefits that migration brings for health in all sectors. Importantly, the report provides the findings from a global expert consultation of key health threats for CMS-listed species. Despite the diversity of species considered, from insects to elephants, and their diverse health threats, there is remarkable similarity in theunderlying drivers of their health threats, namely the human induced pressures outlined above.The key recommendations1 outline the frameworks required to deliver One Health, and how to reduce risks at wildlife interfaces, tackle non-infectious diseases, improve disease prevention and preparedness, fill knowledge gaps, and improve wildlife health reporting and information sharing. These actions will not only improve the conservation status of migratory species, but they will also reduce the health risks to people and livestock.In a climate changing world with an interlinked global biodiversity crisis where the consequences of the current Covid pandemic still playing out, we need no more warnings, we now know now what we have to do to act for the health of us all

Effect of Breed and Gender on Meat Quality of M. longissimus thoracis et lumborum Muscle from Crossbred Beef Bulls and Steers

peer-reviewedAbstract The objective of this study was to determine whether sire breed and/or castration had an effect on meat quality of M. longissimus thoracis et lumborum (LTL) muscle from crossbred bulls and steers and to investigate the relationship amongst the traits examined. Warner–Bratzler shear force (WBSF), intramuscular fat (IMF)%, cook-loss%, drip-loss%, colour (L*, a*, b*) and ultimate pH (upH) were determined in the LTL muscle from eight beef sire breeds representative of the Irish herd (Aberdeen Angus, Belgian Blue, Charolais, Hereford, Limousin, Parthenaise, Salers and Simmental). The results indicate that IMF%, cook-loss% and drip-loss% were associated with breed (p < 0.05); while WBSF, IMF% and cook-loss% differ between genders (p < 0.05). Steer LTL had a greater IMF% and exhibited reduced WBSF and cook-loss% in comparison to the bull LTL (p < 0.05). This study provides greater insight into how quality traits in beef are influenced by breed and gender and will support the industry to produce beef with consistent eating quality

Online Prediction of Physico-Chemical Quality Attributes of Beef Using Visible—Near-Infrared Spectroscopy and Chemometrics

peer-reviewedThe potential of visible–near-infrared (Vis–NIR) spectroscopy to predict physico-chemical quality traits in 368 samples of bovine musculus longissimus thoracis et lumborum (LTL) was evaluated. A fibre-optic probe was applied on the exposed surface of the bovine carcass for the collection of spectra, including the neck and rump (1 h and 2 h post-mortem and after quartering, i.e., 24 h and 25 h post-mortem) and the boned-out LTL muscle (48 h and 49 h post-mortem). In parallel, reference analysis for physico-chemical parameters of beef quality including ultimate pH, colour (L, a*, b*), cook loss and drip loss was conducted using standard laboratory methods. Partial least-squares (PLS) regression models were used to correlate the spectral information with reference quality parameters of beef muscle. Different mathematical pre-treatments and their combinations were applied to improve the model accuracy, which was evaluated on the basis of the coefficient of determination of calibration (R2C) and cross-validation (R2CV) and root-mean-square error of calibration (RMSEC) and cross-validation (RMSECV). Reliable cross-validation models were achieved for ultimate pH (R2CV: 0.91 (quartering, 24 h) and R2CV: 0.96 (LTL muscle, 48 h)) and drip loss (R2CV: 0.82 (quartering, 24 h) and R2CV: 0.99 (LTL muscle, 48 h)) with lower RMSECV values. The results show the potential of Vis–NIR spectroscopy for online prediction of certain quality parameters of beef over different time periods

Investigating the use of visible and near infrared spectroscopy to predict sensory and texture attributes of beef M. longissimus thoracis et lumborum

peer-reviewedThe aim of this study was to calibrate chemometric models to predict beef M. longissimus thoracis et lumborum (LTL) sensory and textural values using visible-near infrared (VISNIR) spectroscopy. Spectra were collected on the cut surface of LTL steaks both on-line and off-line. Cooked LTL steaks were analysed by a trained beef sensory panel as well as undergoing WBSF analysis. The best coefficients of determination of cross validation (R2CV) in the current study were for textural traits (WBSF = 0.22; stringiness = 0.22; crumbly texture = 0.41: all 3 models calibrated using 48 h post-mortem spectra), and some sensory flavour traits (fatty mouthfeel = 0.23; fatty after-effect = 0.28: both calibrated using 49 h post-mortem spectra). The results of this experiment indicate that VISNIR spectroscopy has potential to predict a range of sensory traits (particularly textural traits) with an acceptable level of accuracy at specific post-mortem times.This work is funded by the BreedQuality project (11/SF/311) which is supported by The Irish Department of Agriculture, Food and the Marine (DAFM) under the National Development Plan 2007–2013

Wildlife health outcomes and opportunities in conservation translocations

It is intuitive that the health status of wildlife might influence conservation translocation outcomes, however, health as a topic has received limited attention in the conservation translocation literature. We determined the forms and frequency of disease and other biological problems reported in translocated animals and plants, and in populations linked to translocation, and associations between their mention and translocation ‘success’. From these problems we deduced the forms of ill-health potentially associated with conservation translocation and developed contextual frameworks to inform health management

Prediction of Warner-Bratzler shear force, intramuscular fat, drip-loss and cook-loss in beef via Raman spectroscopy and chemometrics

Rapid prediction of beef quality remains a challenge for meat processors. This study evaluated the potential of Raman spectroscopy followed by chemometrics for prediction of Warner-Bratzler shear force (WBSF), intramuscular fat (IMF), ultimate pH, drip-loss and cook-loss. PLS regression models were developed based on spectra recorded on frozen-thawed day 2 longissimus thoracis et lumborum muscle and validated using test sets randomly selected 3 times. With the exception of ultimate pH, models presented notable performance in calibration (R2 ranging from 0.5 to 0.9; low RMSEC) and, despite variability in the results, promising predictive ability: WBSF (RMSEP ranging from 4.6 to 9 N), IMF (RMSEP ranging from 0.9 to 1.1%), drip-loss (RMSEP ranging from 1 to 1.3%) and cook-loss (RMSEP ranging from 1.5 to 2.9%). Furthermore, the loading values indicated that the physicochemical variation of the meat influenced the models. Overall, results indicated that Raman spectroscopy is a promising technique for routine quality assessments of IMF and drip-loss, which, with further development and improvement of its accuracy could become a reliable tool for the beef industry

Wetlands for wellbeing: piloting a nature-based health intervention for the management of anxiety and depression

Nature-based health interventions (NBIs) for the treatment of poor mental health are becoming increasingly common, yet evidence to support their effectiveness is lacking. We conduct a pilot study of a six-week intervention, aiming to engage individuals with wetland nature for the treatment of anxiety and/or depression. We employed a mixed methods design, using questionnaires, focus groups and semi-structured interviews to evaluate the intervention from the perspective of participants (n = 16) and healthcare professionals (n = 2). Results demonstrate significant improvements in mental health across a range of indicators, including mental wellbeing (Warwick and Edinburgh Mental Wellbeing Scale), anxiety (Generalised Anxiety Disorder-7), stress (Perceived Stress Scale) and emotional wellbeing (Positive and Negative Affect Schedule). Participants and healthcare professionals cited additional outcomes including improved physical health and reduced social isolation. The wetland site provided a sense of escape from participants’ everyday environments, facilitating relaxation and reductions in stress. Wetland staff knowledge of the natural world, transportation and group organisation also played a considerable role in the intervention’s success. These aspects should be considered in future and existing NBIs to maximise benefits to participants. We propose NBIs based in wetlands are an effective therapy option for individuals diagnosed with anxiety and/or depression

Prediction of Warner-Bratzler shear force, intramuscular fat, drip-loss and cook-loss in beef via Raman spectroscopy and chemometrics

peer-reviewedRapid prediction of beef quality remains a challenge for meat processors. This study evaluated the potential of Raman spectroscopy followed by chemometrics for prediction of Warner-Bratzler shear force (WBSF), intramuscular fat (IMF), ultimate pH, drip-loss and cook-loss. PLS regression models were developed based on spectra recorded on frozen-thawed day 2 longissimus thoracis et lumborum muscle and validated using test sets randomly selected 3 times. With the exception of ultimate pH, models presented notable performance in calibration (R2 ranging from 0.5 to 0.9; low RMSEC) and, despite variability in the results, promising predictive ability: WBSF (RMSEP ranging from 4.6 to 9 N), IMF (RMSEP ranging from 0.9 to 1.1%), drip-loss (RMSEP ranging from 1 to 1.3%) and cook-loss (RMSEP ranging from 1.5 to 2.9%). Furthermore, the loading values indicated that the physicochemical variation of the meat influenced the models. Overall, results indicated that Raman spectroscopy is a promising technique for routine quality assessments of IMF and drip-loss, which, with further development and improvement of its accuracy could become a reliable tool for the beef industry.Department of Agriculture, Food and the Marine, Irelan