Genomic heterogeneity and prevalence of hepandensovirus in Penaeus esculentus from Western Australia, and P. merguiensis from the Gulf of Carpentaria, Australia

Decapod Hepandensovirus 1 (HDV), formerly known as hepatopancreatic parvovirus, has been associated with stunting, lowered production and outright mortalities in prawns in aquaculture. Despite the fact that broodstock are sourced and aquaculture farms are planned in the regions of northern and Western Australia, data on these parvoviruses from this region are limited. The prevalence of HDV in Penaeus esculentus and Penaeus merguiensis is moderate (34–51%) in southern Western Australia, Exmouth Gulf and the Gulf of Carpentaria but statistically higher (P < 0.05) in Shark Bay (82%). We speculate this is due to the topography of Shark Bay combined with the currents of the Indian Ocean gyre (IOG). Despite an on average 8–12% genomic heterogeneity, the nucleotide sequences of HDV in WA most closely align with HDV in regions associated with the IOG; Thailand, India, Tanzania, Madagascar; eastern Asia, Korea and less commonly, with sequences from the eastern coast of Australia. This potentially changes the paradigm of a single strain of HDV being ubiquitous in Australia and there was little risk in moving broodstock from WA to the eastern states, so there was no testing of broodstock for HDV. There is no strong evidence to clarify whether the strain of HDV in WA P. esculentus came from either its’ nearest genetic relatives, P. monodon or P. semisulcatus or from P. merguiensis from the Solanderian province of Australia. P. esculentus HDV appears to be most related to strains within the IOG. The HDV nucleotide heterogeneity of wild prawns contrasts strongly to studies undertaken with prawns from aquaculture where genetic selection may have occurred

Population-specific call order in chimpanzee greeting vocal sequences

This study was funded by the Max Planck Society and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program awarded to C.C. (grant agreement no. 679787) and ERC (Prilang GA283871) and by Leverhulme Trust Research Leadership Award. Core funding for the Taï Chimpanzee Project has been provided by the Max Planck Society since 1997 and for Budongo Conservation Field station by the Royal Zoological Society of Scotland.Primates rarely learn new vocalisations, but they can learn to use their vocalizations in different contexts. Such ‘vocal usage learning’, particularly in vocal sequences, is a hallmark of human language, but remains understudied in non-human primates. We assess usage learning in four wild chimpanzee communities of Taï and Budongo Forests by investigating population differences in call ordering of a greeting vocal sequence. Whilst in all groups, these sequences consisted of pant-hoots (long-distance contact call) and pant-grunts (short-distance submissive call), the order of the two calls differed across populations. Taï chimpanzees consistently commenced greetings with pant-hoots whereas Budongo chimpanzees started with pant-grunts. We discuss different hypotheses to explain this pattern and conclude that higher intra-group aggression in Budongo may have led to a local pattern of individuals signalling submission first. This highlights how within-species variation in social dynamics may lead to flexibility in call order production, possibly acquired via usage learning.Publisher PDFPeer reviewe

Urinary Cortisol, Aggression, Dominance and Competition in Wild, West African Male Chimpanzees

High dominance status is associated with fitness benefits in many social mammals. Yet, attaining and maintaining a high social status often comes with elevated energetic costs. Dominance rank-related exposure to energetic and psychosocial stressors is predicted to vary depending on the type of breeding system, the means a high rank is acquired and maintained, and the stability of the dominance hierarchy. Using behavioral data and urinary cortisol levels, we investigated whether a high dominance rank is associated with elevated energetic costs in Taï male chimpanzees and whether the relationship between male dominance rank and cortisol levels varies between stable and unstable dominance periods. Additionally, we investigated potential sources of energetic and psychosocial stress linked to competition over dominance status and mating opportunities. We found that higher-ranking males gave more aggressions than lower-ranking males in stable and unstable dominance periods, but that dominance rank and urinary cortisol levels were not associated in either period. Urinary cortisol levels were higher in all males in unstable compared to stable dominance periods, whereas aggression rates showed the reversed pattern, with higher rates in stable periods. Our results indicate that dominance maintenance is not associated with elevated physiological stress for dominant Taï male chimpanzees, and that social instability exposed all males to psychosocial stress, despite lower rates of aggressive interactions. Overall, these findings suggest that male chimpanzees adjust competitive behavior to context dependent conditions, possibly by means of predictability of outcomes of social interactions, and use conflict management strategies, such as avoidance of aggression potentially diminishing the risk of escalation

Variable use of polyadic grooming and its effect on access to social partners in wild chimpanzees and bonobos

In mammals, allogrooming is prominent in forming and maintaining social and cooperative relationships. Yet an animal's social time is constrained, which may limit its access to a large number of partners. Dunbar (1993, Behavioral and Brain Sciences, 16 (4), 681–694) proposed that human polyadic conversations, which allow access to several social partners simultaneously, evolved as a form of social grooming to circumvent this time constraint. In nonhuman primates, polyadic grooming (PG), in contrast to dyadic grooming, may similarly be a time-efficient way to maintain weak social relationships with many partners which can be important for group level cooperation. It remains unknown whether PG is used to fulfil specific cooperative needs by accessing numerous weakly bonded partners and increasing the number of partners accessed per unit of time. We compared the use and effect of PG between chimpanzees, Pan troglodytes, which are highly territorial and collaborative (especially males), and bonobos, Pan paniscus, which are less territorial and collaborative and in which females are the main co-operators. We carried out focal grooming observations in one bonobo and two chimpanzee communities in the wild. As predicted, chimpanzees engaged in more PG than bonobos. Surprisingly, males engaged in PG more than females in both species. While chimpanzees accessed more partners per minute of grooming than bonobos via dyadic grooming, PG increased the number of partners accessed per minute only in bonobos. Finally, chimpanzees primarily used PG with individuals who were close in rank and frequent grooming partners, whereas bonobos used PG with individuals who were distant in rank, close party associates and frequent grooming partners. We suggest that bonobo males use PG to enhance conspecific social tolerance and mate choice. The overall higher rate of PG in chimpanzees suggests that between-group competition may promote polyadic affiliation, which possibly reinforces group cohesion and coordination. © 2020 The Author

Bridging the Gap Between the Foreland and Hinterland II: Geochronology and Tectonic Setting of Ordovician Magmatism and Basin Formation on the Laurentian Margin of New England and Newfoundland

Ordovician strata of the Mohawk Valley and Taconic allochthon of New York and the Humber margin of Newfoundland record multiple magmatic and basin-forming episodes associated with the Taconic orogeny. Here we present new U-Pb zircon geochronology and whole rock geochemistry and neodymium isotopes from Early Paleozoic volcanic ashes and siliciclastic units on the northern Appalachian margin of Laurentia. Volcanic ashes in the Table Point Formation of Newfoundland and the Indian River Formation of the Taconic allochthon in New York yield dates between 466.16 ± 0.12 and 464.20 ± 0.13 Ma. Red, bioturbated slate of the Indian River Formation record a shift to more juvenile neodymium isotope values suggesting sedimentary contributions from the Taconic arc-system by 466 Ma. Eight ashes within the Trenton Group in the Mohawk Valley were dated between 452.63 ± 0.06 and 450.68 ± 0.12 Ma. These ashes contain zircon with Late Ordovician magmatic rims and 1.4 to 1.0 Ga xenocrystic cores that were inherited from Grenville basement, suggesting that the parent magmas erupted through the Laurentian margin. The new geochronological and geochemical data are integrated with a subsidence model and data from the hinterland to refine the tectonic model of the Taconic orogeny. Closure of the Iapetus Ocean by 475 Ma via collision of the peri-Gondwanan Moretown terrane with hyperextended distal fragments of the Laurentian margin is not clearly manifested on the autochthon or the Taconic allochthon other than an increase in sediment accumulation. Pro-foreland basins formed during the Middle Ordovician when these terranes were obducted onto the Laurentian margin. 466 to 464 Ma ashes on the Laurentian margin coincide with a late pulse of magmatism in both the Notre Dame arc in Newfoundland and the Shelburne Falls arc of New England that is potentially related to break-off of an east-dipping slab. Following slab reversal, by 455 Ma, the Bronson Hill arc was established on the new composite Laurentian margin. Thus, we conclude that Late Ordovician strata in the Mohawk Valley and Taconic allochthon of New York and on the Humber margin of Newfoundland were deposited in retro-foreland basins

A detailed phenotypic analysis of immune cell populations in the bronchoalveolar lavage fluid of atopic asthmatics after segmental allergen challenge

BACKGROUND: Atopic asthma is characterized by intermittent exacerbations triggered by exposure to allergen. Exacerbations are characterized by an acute inflammatory reaction in the airways, with recruitment of both innate and adaptive immune cells. These cell populations as well as soluble factors are critical for initiating and controlling the inflammatory processes in allergic asthma. Detailed data on the numbers and types of cells recruited following allergen challenge is lacking. In this paper we present an extensive phenotypic analysis of the inflammatory cell infiltrate present in the bronchoalveolar lavage (BAL) fluid following bronchoscopically directed allergen challenge in mild atopic asthmatics. METHODS: A re-analysis of pooled data obtained prior to intervention in our randomized, placebo controlled, double blinded study (costimulation inhibition in asthma trial [CIA]) was performed. Twenty-four subjects underwent bronchoscopically directed segmental allergen challenge followed by BAL collection 48 hours later. The BAL fluid was analyzed by multi-color flow cytometry for immune cell populations and multi-plex ELISA for cytokine detection. RESULTS: Allergen instillation induced pro-inflammatory cytokines (IL-6) and immune modulating cytokines (IL-2, IFN-γ, and IL-10) along with an increase in lymphocytes and suppressor cells (Tregs and MDSC). Interestingly, membrane expression of CD30 was identified on lymphocytes, especially Tregs, but not eosinophils. Soluble CD30 was also detected in the BAL fluid after allergen challenge in adult atopic asthmatics. CONCLUSIONS: After segmental allergen challenge of adult atopic asthmatics, cell types associated with a pro-inflammatory as well as an anti-inflammatory response are detected within the BAL fluid of the lung

Early maternal loss leads to short-but not long-term effects on diurnal cortisol slopes in wild chimpanzees

The biological embedding model (BEM) suggests that fitness costs of maternal loss arise when early-life experience embeds long-term alterations to hypothalamic-pituitary-adrenal (HPA) axis activity. Alternatively, the adaptive calibration model (ACM) regards physiological changes during ontogeny as short-term adaptations. Both models have been tested in humans but rarely in wild, long-lived animals. We assessed whether, as in humans, maternal loss had short-and long-term impacts on orphan wild chimpanzee urinary cortisol levels and diurnal urinary cortisol slopes, both indicative of HPA axis functioning. Immature chimpanzees recently orphaned and/or orphaned early in life had diurnal cortisol slopes reflecting heightened activation of the HPA axis. However, these effects appeared short-term, with no consistent differences between orphan and non-orphan cortisol profiles in mature males, suggesting stronger support for the ACM than the BEM in wild chimpan-zees. Compensatory mechanisms, such as adoption, may buffer against certain physiological effects of maternal loss in this species

A comparative study of autistic and non-autistic women’s experience of motherhood

Abstract: Background: Autism is a lifelong neurodevelopmental difference and disability, yet there is limited research examining parenting in autistic mothers. Objective: To explore autistic mothers’ experience of the perinatal period and parenthood. This includes pregnancy, childbirth, the postpartum period, self-perception of parenting strengths and weaknesses, communication with professionals in relation to one’s child, mental health difficulties and the social experience of motherhood. It also includes disclosing one’s diagnosis of autism in parenting contexts. Methods: We used a community-based participatory research model, and recruited an advisory panel, with whom we co-developed an anonymous, online survey for autistic mothers. The online survey was completed by autistic and non-autistic mothers, and we compared their responses using Chi-squared analysis. Sample: Autistic mothers (n = 355), and non-autistic mothers (n = 132), each of whom had at least one autistic child, were included in our final analysis. Results: There were differences in education, gender identity and age of mother at birth of first child. Autistic mothers were more likely to have experienced additional psychiatric conditions, including pre- or post-partum depression, and reported greater difficulties in areas such as multi-tasking, coping with domestic responsibilities and creating social opportunities for their child. They were also more likely to report feeling misunderstood by professionals, and reported greater anxiety, higher rates of selective mutism, and not knowing which details were appropriate to share with professionals. They were also more likely to find motherhood an isolating experience, to worry about others judging their parenting, or feel unable to turn to others for support in parenting. However, despite these challenges, autistic mothers were able to act in the best interest of their child, putting their child’s needs first. Conclusions: Autistic mothers face unique challenges and the stigma associated with autism may further exacerbate communication difficulties. Greater understanding and acceptance amongst individuals who interact with autistic mothers is needed, and autistic mothers would benefit from additional and better-tailored support

Quantifying within-group variation in sociality—covariation among metrics and patterns across primate groups and species

It has long been recognized that the patterning of social interactions within a group can give rise to a social structure that holds very different places for different individuals. Such within-group variation in sociality correlates with fitness proxies in fish, birds, and mammals. Broader integration of this research has been hampered by the lack of agreement on how to integrate information from a plethora of dyadic interactions into individual-level metrics. As a step towards standardization, we collected comparative data on affinitive and affiliative interactions from multiple groups each of five species of primates to assess whether the same aspects of sociality are measured by different metrics and indices. We calculated 16 different sociality metrics used in previous research and thought to represent three different sociality concepts. We assessed covariation of metrics within groups and then summarized covariation patterns across all 15 study groups, which varied in size from 5 to 41 adults. With some methodological and conceptual caveats, we found that the number of weak ties individuals formed within their groups represented a dimension of sociality that was largely independent from the overall number of ties as well as from the number and strength of the strong ties they formed. Metrics quantifying indirect connectedness exhibited strong covariation with strong tie metrics and thus failed to capture a third aspect of sociality. Future research linking affiliation and affinity to fitness or other individual level outcomes should quantify inter-individual variation in three aspects: the overall number of ties, the number of weak ties, and the number or strength of strong ties individuals form, after taking into account effects of social network density. Significance statement: In recent years, long-term studies of individually known animals have revealed strong correlations between individual social bonds and social integration, on the one hand, and reproductive success and survival on the other hand, suggesting strong natural selection on affiliative and affinitive behavior within groups. It proved difficult to generalize from these studies because they all measured sociality in slightly different ways. Analyzing covariation between 16 previously used metrics identified only three rather independent dimensions of variation. Thus, different studies have tapped into the same biological phenomenon. How individuals are weakly connected within their group needs further attention.Peer Reviewe