Blackbody temperature of 200+ stellar flares observed with the CoRoT satellite

We estimated blackbody temperature for 209 flares observed at 69 F-K stars, significantly increasing the number of flare temperature determinations. We used the Blue and Red channels obtained by the 27 cm telescope of the CoRoT satellite at high cadence and long duration. The wavelength limits of the channels were estimated using spectra from the Pickles library for the spectral type and luminosity class of each star, provided by the Exodat Database. The temperatures were obtained from the flare energy Blue-to-Red ratio, using the flare equivalent duration and stellar flux in both channels. The expected value of the analyzed flares is equal to 6,400 K with a standard deviation of 2,800 K, where the mean stellar spectral type, weighted by the number of flares in each spectral subclass, is equal to G6. Contrary to our results, a stellar white-light flare is often assumed to emit as a blackbody with a temperature of 9,000 K or 10,000 K. Our estimates agree, however, with values obtained for solar flares. The GAIA G-band transmissivity is comparable to that of the CoRoT White channel, which allows us to calibrate the flares to the Gaia photometric system. The energy in the G band of the analyzed flares varies between $10^{32}$ and $10^{37}$ erg and the flare area ranges from 30$\mu$sh to 3 sh (solar hemisphere). The energy release per area in a flare is proportional to $T_{\rm flare}^{2.6}$, at least up to 10,000 K.Comment: Accepted Astronomical Journa

Perturbações alimentares em portugal: padrões de utilização dos serviços

As perturbações alimentares (anorexia nervosa e bulimia nervosa) são problemas psicopatológicos sérios que afectam principalmente as mulheres jovens. Neste âmbito, Portugal participou num projecto de investigação de europeu, enquadrado nas acções do COST — Cooperation on Science and Technology, sobre a eficácia da psicoterapia das perturbações alimentares, a Acção COST-B6. O presente artigo apresenta uma caracterização dos utentes que recorreram a um destes centros de tratamento especializado no Norte, Centro ou Sul do país, durante o período do estudo. Durante o estudo 170 pacientes do sexo feminino, diagnosticados com uma Anorexia Nervosa ou Bulimia Nervosa de acordo com os critérios de diagnóstico do DSM-IV, iniciaram o tratamento neste período. Os pacientes responderam a vários instrumentos de avaliação clínica antes do início do tratamento. Os resultados mostraram que uma proporção considerável dos utentes é jovem e que os índices de gravidade dos sintomas demonstram um considerável comprometimento do nível de funcionamento esperado. Tal como esperado os pacientes com perturbação do comportamento alimentar apresentam resultados, nas escalas de avaliação das perturbações alimentares, superiores aos da população normal, confirmando a gravidade dos sintomas no momento da amissão ao tratamento. Curiosamente, os pacientes bulímicos tendem a apresentar resultados mais elevados nestas escalas clínicas, sugerindo um nível mais elevado nível de sofrimento subjectivo. Por último, a proporção dos pacientes com bulimia nervosa e anorexia nervosa que se apresentaram ao tratamento são semelhantes, o que poderá sugerir que um número significativo de pacientes bulímicos não recebe tratamento especializado.Fundação para a Ciência e a Tecnologia (FCT) - PRAXIS/PCSH/P/PSI/85/9

Contribution of different vegetable types to exogenous nitrate and nitrite exposure

This study reports the levels of nitrate and nitrite of 34 vegetable samples, including different varieties of cabbage, lettuce, spinaches, parsley and turnips, collected in several locations of an intensive agricultural area (Modivas, Vila do Conde, northern Portugal). Nitrate levels ranged between 54 and 2440 mg NO-3 kg-1, while nitrite levels ranged between 1.1 and 57 mg NO-2 kg-1. The maximum residue levels established for nitrate in spinach and lettuce samples were not exceeded. Nitrate and nitrite levels reported in the literature for the same type of samples are reviewed, as well as the contribution of vegetables to nitrate and nitrite dietary exposure of populations

Differences in chemical composition and antioxidant activity of three propolis samples collected in the same apiary

Financial support provided by FCT (PD/BD/128276/2017), under the Doctoral Programme Agrichains - PD/00122/2012

Identification of genomic loci associated with genotypic and phenotypic variation among Pseudomonas aeruginosa clinical isolates from pneumonia

In this work, a genotype-phenotype survey of a highly diversified Pseudomonas aeruginosa collection was conducted, aiming to detail pathogen-associated scenarios that clinicians face nowadays. Genetic relation based on RAPD-PCR of 705 isolates, retrieved from 424 patients and several clinical contexts, reported an almost isolate-specific molecular-pattern. Pneumonia-associated isolates HB13 and HB15, clustered in the same RAPD-PCR group, were selected to evaluate the genomic background underlying their contrasting antibiotic resistance and virulence. The HB13 genome harbors antibiotic-inactivating enzymes-coding genes (e.g. aac(3)-Ia, arr, blaVIM-2) and single-nucleotide variations (SNVs) in antibiotic targets, likely accounting for its pan-resistance, whereas HB15 susceptibility correlated to predicted dysfunctional alleles. Isolate HB13 showed the unprecedented rhl-cluster absence and variations in other pathogen competitiveness contributors. Conversely, HB15 genome comprises exoenzyme-coding genes and SNVs linked to increased virulence. Secretome analysis identified signatures features with unknown function as potential novel pathogenic (e.g. a MATE-protein in HB13, a protease in HB15) and antibiotic resistance (a HlyD-like secretion protein in HB13) determinants. Detection of active prophages, proteases (including protease IV and alkaline metalloproteinase), a porin and a peptidase in HB15 highlights the secreted arsenal likely essential for its virulent behavior. The presented phenotype-genome association will contribute to the current knowledge on Pseudomonas aeruginosa pathogenomics.This work was supported by the strategic programme UID/BIA/0050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through FCT I.P., by ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) and through a PhD grant (SFRH/BD/98558/2013) attributed to C.S.M. The facility for Biological Mass Spectrometry Isabel Moura was funded by Proteomass Scientific Society. H.M.S. is funded by the FCT 2015 Investigator Program (IF/00007/2015)

Vaccination of Mice with Salmonella Expressing VapA: Mucosal and Systemic Th1 Responses Provide Protection against Rhodococcus equi Infection

Conventional vaccines to prevent the pneumonia caused by Rhodococcus equi have not been successful. We have recently demonstrated that immunization with Salmonella enterica Typhimurium expressing the VapA antigen protects mice against R. equi infection. We now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific IgA, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation with R. equi antigen. After in vitro stimulation, spleen cells of immunized mice produce high levels of Th1 cytokines and show a prominent mRNA expression of the Th1 transcription factor T-bet, in detriment of the Th2 transcription factor GATA-3. Following R. equi challenge, a high H2O2, NO, IL-12, and IFN-γ content is detected in the organs of immunized mice. On the other hand, TNF-α and IL-4 levels are markedly lower in the organs of vaccinated mice, compared with the non-vaccinated ones. The IL-10 content and the mRNA transcription level of TGF-β are also higher in the organs of immunized mice. A greater incidence of CD4+ and CD8+ T cells and B lymphocytes is verified in vaccinated mice. However, there is no difference between vaccinated and non-vaccinated mice in terms of the frequency of CD4+CD25+Foxp3+ T cells. Finally, we show that the vaccination confers a long-term protection against R. equi infection. Altogether, these data indicate that the oral vaccination of mice with S. enterica Typhimurium expressing VapA induces specific and long-lasting humoral and cellular responses against the pathogen, which are appropriately regulated and allow tissue integrity after challenge

Bioactive lipids of seaweeds from the Portuguese north coast: health benefits versus potential contamination

The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.info:eu-repo/semantics/publishedVersio

Soybean growth and production under straw of maize, Urochloa brizantha, Conyza spp. and Digitaria insularis

Weeds can cause serious damage during soybean development, due to allelopathy, competition for water, light and nutrients. It is necessary to investigate the influence of straw, of weeds Conyza spp. and Digitaria insularis, in soybean growth, production and composition and grains. If there is influence of allelopathic compounds at the crop. The aim of this study was to evaluate the influence of straw of maize and Urochloa brizantha (A.Rich.) R.D.Webster crops and Conyza spp., D. insularis, on growth, production and composition of grains produced by soybean. Treatments consisted of the control (absence of straw), maize straw, Urochloa brizantha straw, Conyza spp. straw (500, 1000, 1500 or 2000 kg ha-1) and D. insularis straw (500, 1000, 1500 or 2000 kg ha-1). The chlorophyll index, height of plants and insertion of the first pod, stem diameter at the collar and at 5 cm from the collar, root dry mass, number of pods and grains, weight of total grains, weight of 100 grains, protein and nitrogen (N), catalase and peroxidase contents in grains were evaluated. There was no difference between treatments for plant height, first pod height and chlorophyll index, as well as for total pods and 100 grain weight and protein content, N content and peroxidase and carboxylase enzymatic activity of the grains produced. For stem diameter, a higher value was found for the treatment with maize straw compared to the control (no straw). For dry root matter, treatments without straw and with Conyza spp. straw up to 1500 kg ha-1, differed from the treatment with maize straw. Even in some respects they provided beneficial effects compared to the absence of straw, which indicates the importance of crop residues. No allelopathic effects of weeds were observed on the growth and development of soybean. Conyza spp., D. insularis, maize or U. brizantha straws do not negatively affect soybean growth, production and grain composition

Nursing practice environment in intensive care units

Objetivo Analisar o ambiente da prática de enfermagem em unidades de terapia intensiva. Métodos Estudo descritivo, de abordagem quantitativa, realizado com 209 profissionais de enfermagem de três hospitais de ensino brasileiros. O ambiente da prática de enfermagem foi avaliado através da Practice Environment Scale. Os dados foram analisados descritivamente, assumindo nível de significância de 5% (p<0,05). O coeficiente Alfa de Cronbach foi utilizado para examinar a consistência interna dos construtos. Resultados Os profissionais de enfermagem consideraram desfavoráveis quatro das cinco dimensões do ambiente da prática profissional: participação dos enfermeiros na discussão dos assuntos hospitalares; fundamentos de enfermagem voltados para a qualidade do cuidado, habilidade, liderança e suporte dos coordenadores/supervisores de enfermagem aos enfermeiros/equipe de enfermagem; e adequação da equipe e de recursos. Apenas a dimensão relações colegiais entre profissionais de enfermagem e médicos apresentou avaliação positiva. Enfermeiros reconheceram mais fortemente atributos desfavoráveis no ambiente de prática do que técnicos de enfermagem. Conclusão O ambiente mostrou-se desfavorável para a prática dos profissionais de enfermagem. Esforços são necessários para tornar o ambiente de prática mais atrativo aos profissionais de enfermagem, e assim estimular melhorias na qualidade e na segurança da assistência prestada.Objective To analyze the nursing practice environment in intensive care units. Methods This is a descriptive study with a quantitative approach, carried out with 209 nursing professionals of three Brazilian teaching hospitals. The nursing work environment was evaluated using the Practice Environment Scale. Data were analyzed descriptively, assuming a significance level of 5% (p<0.05). Cronbach’s alpha coefficient was used to assess the internal consistency of the constructs. Results Nurse professionals considered unfavorable four of the five professional practice environment dimensions: nurse participation in hospital affairs, nursing foundation for quality of care, nurse manager ability, leadership and support of nurses and staffing and resources adequacy. Only the dimension of collegial nurse-physician relations presented a positive evaluation. Unfavorable characteristics of the working environment were recognized more strongly by nurses in comparison to nursing technicians. Conclusion The environment proved to be unfavorable for the practice of nursing professionals. Efforts are necessary to make the work environment more attractive to them, thus stimulating improvements in the quality and safety of care delivered to patients.Objetivo Analizar el ambiente de la práctica de enfermería en unidades de terapia intensiva. Métodos Estudio descriptivo, de abordaje cuantitativo, realizado con 209 profesionales de enfermería de tres hospitales de enseñanza brasileños. El ambiente de la práctica de enfermería fue evaluado utilizándose la Practice Environment Scale. Datos analizados descriptivamente, asumiéndose nivel de significatividad de 5% (p<0,05). Se utilizó coeficiente Alfa de Cronbach para examinar la consistencia interna de los constructos. Resultados Los profesionales de enfermería consideran desfavorables cuatro de las cinco dimensiones del ambiente de la práctica profesional: participación de enfermeros en discusión de asuntos hospitalarios; fundamentos de enfermería orientados a calidad del cuidado; habilidad, liderazgo y soporte de coordinadores/supervisores de enfermería a los enfermeros/equipo de enfermería; y adecuación del equipo y de recursos. Solamente la dimensión relaciones entre profesionales de enfermería y médicos mostró evaluación positiva. Los enfermeros reconocieron más sólidamente atributos desfavorables en el ambiente de práctica que los auxiliares de enfermería. Conclusión El ambiente se mostró desfavorable para la práctica de los profesionales de enfermería. Son necesarios esfuerzos para que el ambiente de práctica sea más atractivo para los profesionales de enfermería, estimulando así mejoras en calidad y seguridad de la atención brindada

Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma

Gliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis. In a first view, STI1, hnRNPs and PGK1 are under expressed on CGl. Similarly, both carbonic anhydrase and aldose reductase are even suppressed in glioma cells that grown under adipokines-enriched environment. Contrariwise, RFC1, KIF5C, ANXA2, N-RAP and RACK1 are overexpressed in GL261 cell the in the presence of the adipokines-enriched medium. We further identified the factors that are released by adipocyte cells, and revealed that several pro-inflammatory and angiogenic factors, such as IL-6, IL-11, LIF, PAI-1, TNF-α, endocan, HGF, VEGF IGF-I, were secreted to the medium into a high extent, whereas TIMP-1 and SerpinE1 were under expressed on CGl. This study discloses an interesting in vitro model for the study of glioma biology under a "obesity" environment, that can be explored for the understanding of cancer cells biology, for the search of biomarkers, prognostic markers and therapeutic approaches.info:eu-repo/semantics/publishedVersio