36 research outputs found
Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury
[EN]Using an immunohistochemical technique, we have studied the distribution of kynuneric acid (KYNA) and 3-hydroxyanthranilic acid (3-HAA) in a rat brain injury model (trauma). The study was carried out inducing a cerebral ablation of the frontal motor cortex. Two mouse monoclonal specific antibodies previously developed by our group directed against KYNA and 3-HAA were used. In control animals (sham-operated), the expression of both KYNA and 3-HAA was not observed. In animals in which the ablation was performed, the highest number of immunoreactive cells containing KYNA or 3-HAA was observed in the region surrounding the lesion and the number of these cells decreased moving away from the lesion. KYNA and 3-HAA were also observed in the white matter (ipsilateral side) located close to the injured region and in some cells placed in the white matter of the contralateral side. The distribution of KYNA and 3-HAA perfectly matched with the peripheral injured regions. The results found were identical independently of the perfusion date of animals (17, 30 or 54 days after brain injury). For the first time, the presence of KYNA and 3-HAA has been described in a rat trauma model
Low-count monoclonal B-cell lymphocytosis persists after seven years of follow up and is associated with a poorer outcome
[EN]Low-count monoclonal B-cell lymphocytosis is defined by the presence
of very low numbers of circulating clonal B cells, usually phenotypically
similar to chronic lymphocytic leukemia cells, whose
biological and clinical significance remains elusive. Herein, we re-evaluated
65/91 low-count monoclonal B-cell lymphocytosis cases (54 chronic
lymphocytic leukemia-like and 11 non-chronic lymphocytic leukemialike)
followed-up for a median of seven years, using high-sensitivity
flow cytometry and interphase fluorescence in situ hybridization.
Overall, the clone size significantly increased in 69% of low-count monoclonal
B-cell lymphocytosis cases, but only one subject progressed to
high-count monoclonal B-cell lymphocytosis. In parallel, the frequency
of cytogenetic alterations increased over time (32% vs. 61% of cases,
respectively). The absolute number of the major T-cell and natural killer
cell populations also increased, but only among chronic lymphocytic
leukemia-like cases with increased clone size vs. age- and sex-matched
controls. Although progression to chronic lymphocytic leukemia was
not observed, the overall survival of low-count monoclonal B-cell lymphocytosis
individuals was significantly reduced vs. non-monoclonal Bcell
lymphocytosis controls (P=0.03) plus the general population from
the same region (Pâ€0.001), particularly among females (P=0.01); infection
and cancer were the main causes of death in low-count monoclonal
B-cell lymphocytosis. In summary, despite the fact that mid-term progression
from low-count monoclonal B-cell lymphocytosis to high-count
monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia
appears to be unlikely, these clones persist at increased numbers, usually
carrying more genetic alterations, and might thus be a marker of an
impaired immune system indirectly associated with a poorer outcome,
particularly among females
Psychometric Properties of the Multidimensional Assessment of Interoceptive Awareness (MAIA) Questionnaire in Colombian University Students
Exploring the Link between Interoceptive Body Awareness and Suicidal Orientation in University Students: A Cross-Sectional Study
Analysis of the Attitudes towards Sexuality in People with Intellectual Disabilities: A Cross-Sectional Study
The barriers faced by people with intellectual disabilities are many. One of the areas in which many problems have been identified is the sexual domain. This descriptive study aims to analyze the attitudes of the family environment, professional carers, and the general population toward their sexuality. A cross-sectional descriptive study was carried out between 2022 and 2023, using convenience sampling among family members and carers from different centers working with people with intellectual disabilities in Spain, and among the general population not related to people with intellectual disabilities. A total of 583 responses were received and significant differences were found for all variables, with the variables related to family or work proximity being those that provided the most significant and relevant results. It was observed that the male sex has a more paternalistic attitude and that in rural areas there is a more permissive attitude towards the sexuality of people with intellectual disabilities. People who work with people with disabilities have more positive attitudes towards this group, while direct relatives have more paternalistic attitudes. Nursing care in the community and specialized centers should be based on an adequate therapeutic relationship and personalized care
Intermediate Molecular Phenotypes to Identify Genetic Markers of Anthracycline-Induced Cardiotoxicity Risk.
Cardiotoxicity due to anthracyclines (CDA) affects cancer patients, but we cannot predict who may suffer from this complication. CDA is a complex trait with a polygenic component that is mainly unidentified. We propose that levels of intermediate molecular phenotypes (IMPs) in the myocardium associated with histopathological damage could explain CDA susceptibility, so variants of genes encoding these IMPs could identify patients susceptible to this complication. Thus, a genetically heterogeneous cohort of mice (n = 165) generated by backcrossing were treated with doxorubicin and docetaxel. We quantified heart fibrosis using an Ariol slide scanner and intramyocardial levels of IMPs using multiplex bead arrays and QPCR. We identified quantitative trait loci linked to IMPs (ipQTLs) and cdaQTLs via linkage analysis. In three cancer patient cohorts, CDA was quantified using echocardiography or Cardiac Magnetic Resonance. CDA behaves as a complex trait in the mouse cohort. IMP levels in the myocardium were associated with CDA. ipQTLs integrated into genetic models with cdaQTLs account for more CDA phenotypic variation than that explained by cda-QTLs alone. Allelic forms of genes encoding IMPs associated with CDA in mice, including AKT1, MAPK14, MAPK8, STAT3, CAS3, and TP53, are genetic determinants of CDA in patients. Two genetic risk scores for pediatric patients (n = 71) and women with breast cancer (n = 420) were generated using machine-learning Least Absolute Shrinkage and Selection Operator (LASSO) regression. Thus, IMPs associated with heart damage identify genetic markers of CDA risk, thereby allowing more personalized patient management.J.P.L.âs lab is sponsored by Grant PID2020-118527RB-I00 funded by MCIN/AEI/10.13039/
501100011039; Grant PDC2021-121735-I00 funded by MCIN/AEI/10.13039/501100011039 and by
the âEuropean Union Next Generation EU/PRTRâ, the Regional Government of Castile and LeĂłn
(CSI144P20). J.P.L. and P.L.S. are supported by the Carlos III Health Institute (PIE14/00066). AGN
laboratory and human patientsâ studies are supported by an ISCIII project grant (PI18/01242). The
Human Genotyping unit is a member of CeGen, PRB3, and is supported by grant PT17/0019 of the
PE I + D + i 2013â2016, funded by ISCIII and ERDF. SCLl is supported by MINECO/FEDER research
grants (RTI2018-094130-B-100). CH was supported by the Department of Defense (DoD) BCRP,
No. BC190820; and the National Cancer Institute (NCI) at the National Institutes of Health (NIH),
No. R01CA184476. Lawrence Berkeley National Laboratory (LBNL) is a multi-program national
laboratory operated by the University of California for the DOE under contract DE AC02-05CH11231.
The Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019/0023 of
the PE I + D +i, 2017â2020, funded by ISCIII and FEDER. RCC is funded by fellowships from
the Spanish Regional Government of Castile and LeĂłn. NGS is a recipient of an FPU fellowship
(MINECO/FEDER). hiPSC-CM studies were funded in part by the âla Caixaâ Banking Foundation
under the project code HR18-00304 and a Severo Ochoa CNIC Intramural Project (Exp. 12-2016
IGP) to J.J.S
Association Between Preexisting Versus Newly Identified Atrial Fibrillation and Outcomes of Patients With Acute Pulmonary Embolism
Background Atrial fibrillation (AF) may exist before or occur early in the course of pulmonary embolism (PE). We determined the PE outcomes based on the presence and timing of AF. Methods and Results Using the data from a multicenter PE registry, we identified 3 groups: (1) those with preexisting AF, (2) patients with new AF within 2 days from acute PE (incident AF), and (3) patients without AF. We assessed the 90-day and 1-year risk of mortality and stroke in patients with AF, compared with those without AF (reference group). Among 16 497 patients with PE, 792 had preexisting AF. These patients had increased odds of 90-day all-cause (odds ratio [OR], 2.81; 95% CI, 2.33-3.38) and PE-related mortality (OR, 2.38; 95% CI, 1.37-4.14) and increased 1-year hazard for ischemic stroke (hazard ratio, 5.48; 95% CI, 3.10-9.69) compared with those without AF. After multivariable adjustment, preexisting AF was associated with significantly increased odds of all-cause mortality (OR, 1.91; 95% CI, 1.57-2.32) but not PE-related mortality (OR, 1.50; 95% CI, 0.85-2.66). Among 16 497 patients with PE, 445 developed new incident AF within 2 days of acute PE. Incident AF was associated with increased odds of 90-day all-cause (OR, 2.28; 95% CI, 1.75-2.97) and PE-related (OR, 3.64; 95% CI, 2.01-6.59) mortality but not stroke. Findings were similar in multivariable analyses. Conclusions In patients with acute symptomatic PE, both preexisting AF and incident AF predict adverse clinical outcomes. The type of adverse outcomes may differ depending on the timing of AF onset.info:eu-repo/semantics/publishedVersio
CriÌtica, periodismo y divulgacioÌn musical en espacios digitales
Mediante el presente proyecto se ha ofrecido formacioÌn en recursos y praÌcticas de criÌtica y periodismo musical, profundizando en la importancia de la divulgacioÌn en espacios digitales. Se trata de la continuacioÌn del anterior proyecto de innovacioÌn no 21 "Herramientas de comunicacioÌn y divulgacioÌn musical orientadas a la transferencia: entornos digitales 2.0". El nuevo proyecto se ha centrado en los aÌmbitos de la criÌtica y el periodismo, concretamente en la actualidad de la prensa escrita y la radio, asiÌ como ha tenido en cuenta el uso extendido de redes sociales y plataformas online en tareas de divulgacioÌn de contenidos. Dicha formacioÌn ha querido dar respuesta a la necesidad de que el profesorado y el alumnado en MusicologiÌa actualice y refuerce su conocimiento sobre los coÌdigos, recursos, y entornos en los que se desarrollan estos campos, ya que se trata actualmente de uno de los aÌmbitos principales de proyeccioÌn profesional de los egresados de MusicologiÌa, a traveÌs de la demanda de publicaciones digitales que requieren especializacioÌn en muÌsica, , radios - en las cuales la interaccioÌn con el usuario de internet es crucial-, e instituciones culturales y musicales que necesitan resenÌas, croÌnicas, y breves escritos enfocados a sus espacios de difusioÌn y promocioÌn en internet. Se trata de un proyecto interfacultativo e interdepartamental ya que ha implicado a profesionales, docentes, investigadores y alumnado del Departamento de MusicologiÌa, el ICCMU (ambos de la Facultad de GeografiÌa e Historia), y el Departamento de Periodismo y Nuevos medios (Facultad de Ciencias de la InformacioÌn)