1,139 research outputs found
A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors
© 2017 Elsevier B.V. This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (riboflavin) and B6 (pyridoxine) in phosphate buffer were established using cyclic voltammetry in conjunction with screen printed carbon electrodes (SPCEs). The optimum current response for all three vitamins was achieved in 0.1 M phosphate buffer pH 11 using an initial potential of −1.0 V. Using square wave voltammetry, the linear ranges for thiamine, riboflavin, and pyridoxine were found to be: 15–110 µg/ml, 0.1–20 µg/ml, and 2–80 µg/ml respectively. The application of the method to a commercial food product yielded a recovery of 95.78% for riboflavin, with a coefficient of variation (CV) of 3.38% (n = 5). The method was also applied to a multi-vitamin supplement for the simultaneous determination of thiamine, riboflavin and pyridoxine. In both cases only simple dilution with buffer followed by centrifugation was required prior to analysis. The resulting square wave voltammetric signals were completely resolved with Ep values of −0.7 V, +0.2 V, and +0.6 V respectively. The recoveries determined for the vitamin B complex in a commercial supplement product were found to be 110%, 114%, and 112% respectively (CV = 7.14%, 6.28%. 5.66% respectively, n = 5)
Intensity of Brillouin light scattering from spin waves in magnetic multilayers with noncollinear spin configurations: Theory and experiment
The scattering of photons from spin waves (Brillouin light scattering -- BLS)
is a well-established technique for the study of layered magnetic systems. The
information about the magnetic state and properties of the sample is contained
in the frequency position, width, and intensity of the BLS peaks. Previously
[Phys. Rev. B 67, 184404 (2003)], we have shown that spin wave frequencies can
be conveniently calculated within the ultrathin film approach, treating the
intralayer exchange as an effective bilinear interlayer coupling between thin
virtual sheets of the ferromagnetic layers. Here we give the consequent
extension of this approach to the calculation of the Brillouin light scattering
(BLS) peak intensities. Given the very close relation of the BLS cross-section
to the magneto-optic Kerr effect (MOKE), the depth-resolved longitudinal and
polar MOKE coefficients calculated numerically via the usual magneto-optic
formalism can be employed in combination with the spin wave precessional
amplitudes to calculate full BLS spectra for a given magnetic system. This
approach allows an easy calculation of BLS intensities even for noncollinear
spin configurations including the exchange modes. The formalism is applied to a
Fe/Cr/Fe/Ag/Fe trilayer system with one antiferromagnetically coupling spacer
(Cr). Good agreement with the experimental spectra is found for a wide variety
of spin configurations.Comment: 19 pages, 5 figure
An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements
An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its electrochemically active thiolate anion species. It was shown that an electrocatalytic oxidation reaction occurred with the screen-printed carbon electrode containing the mediator cobalt phthalocyanine (CoPC-SPCE). This had the advantage of producing an analytical response current at an operating potential of 0 V vs. Ag/AgCl compared to +0.34 V obtained with plain SPCEs. This resulted in improved selectivity and limit of detection. Detailed studies on the underlying mechanism occurring with the sensor are reported in this paper. A linear response was obtained between 0.1 and 20 µg mL −1 , which was suitable for the quantification of the vitamin in two commercial products containing vitamin B1. The mean recovery for a multivitamin tablet with a declared content of 5 mg was 101% (coefficient of variation (CV) of 9.6%). A multivitamin drink, which had a much lower concentration of vitamin B1 (0.22 mg/100 mL), gave a mean recovery of 93.3% (CV 7.2%). These results indicate that our sensor holds promise for quality control of food supplements and other food types
Evolving outer heliosphere: Large-scale stability and time variations observed by the Interstellar Boundary Explorer
The first all-sky maps of Energetic Neutral Atoms (ENAs) from the Interstellar Boundary Explorer (IBEX) exhibited smoothly varying, globally distributed flux and a narrow ribbon of enhanced ENA emissions. In this study we compare the second set of sky maps to the first in order to assess the possibility of temporal changes over the 6 months between views of each portion of the sky. While the large-scale structure is generally stable between the two sets of maps, there are some remarkable changes that show that the heliosphere is also evolving over this short timescale. In particular, we find that (1) the overall ENA emissions coming from the outer heliosphere appear to be slightly lower in the second set of maps compared to the first, (2) both the north and south poles have significantly lower (similar to 10-15%) ENA emissions in the second set of maps compared to the first across the energy range from 0.5 to 6 keV, and (3) the knot in the northern portion of the ribbon in the first maps is less bright and appears to have spread and/or dissipated by the time the second set was acquired. Finally, the spatial distribution of fluxes in the southernmost portion of the ribbon has evolved slightly, perhaps moving as much as 6 degrees (one map pixel) equatorward on average. The observed large-scale stability and these systematic changes at smaller spatial scales provide important new information about the outer heliosphere and its global interaction with the galaxy and help inform possible mechanisms for producing the IBEX ribbon
In flight performance and first results of FREGATE
The gamma-ray detector of HETE-2, called FREGATE, has been designed to detect
gamma-ray bursts in the energy range [6-400] keV. Its main task is to alert the
other instruments of the occurrence of a gamma-ray burst (GRB) and to provide
the spectral coverage of the GRB prompt emission in hard X-rays and soft
gamma-rays. FREGATE was switched on on October 16, 2000, one week after the
successful launch of HETE-2, and has been continuously working since then. We
describe here the main characteristics of the instrument, its in-flight
performance and we briefly discuss the first GRB observations.Comment: Invited lecture at the Woods Hole 2001 GRB Conference, 8 pages, 15
figure
Eukaryotic initiation factor-4E in superficial and muscle invasive bladder cancer and its correlation with vascular endothelial growth factor expression and tumour progression
Vascular endothelial growth factor (VEGF) is an important factor mediating tumour angiogenesis. VEGF mRNA is differentially expressed in bladder cancer with high expression in superficial tumours (stage pTaand pT1) contrasting with low expression in muscle invasive tumours (stage ≥ pT2). To investigate mechanisms regulating VEGF expression in bladder cancer, VEGF mRNA and protein were measured in normal bladder (n = 12) and primary bladder cancers (n = 57). VEGF protein levels correlated with mRNA expression in normal bladder (r = 0.68, P = 0.02) and bladder cancer (r = 0.46, P = 0.0007). Whilst VEGF mRNA expression was threefold higher in superficial compared to muscle invasive bladder cancers (P = 0.0001) there was no difference in VEGF protein (P = 0.81). Accordingly, the median protein:mRNA ratios increased more than 15-fold with increasing tumour stage (P< 0.0001) suggesting translational regulation. Expression of the eukaryotic initiation factor-4E (eIF-4E), a factor implicated in the translational regulation of VEGF, was greater in tumours than normal bladder (P< 0.0001) and correlated with VEGF protein:mRNA ratios (n = 43, r = 0.54, P = 0.0004) pointing to its role in the regulation of VEGF. In superficial tumours (n = 37) high expression of eIF-4E was associated with a poor prognosis and reduced stage progression-free survival (P = 0.04, Cox proportional hazards model). The study demonstrates that eIF-4E may be involved in translational regulation of VEGF in bladder cancer and might have a role as a prognostic factor in bladder cancer. © 2000 Cancer Research Campaig
The hypoxia-inducible genes VEGF and CA9 are differentially regulated in superficial vs invasive bladder cancer
Regulation by hypoxia may underlie the expression of vascular endothelial growth factor in bladder cancer. We have compared the distribution of vascular endothelial growth factor mRNA with a hypoxia marker, carbonic anhydrase 9 (CA IX). vascular endothelial growth factor mRNA was analysed by in situ hybridisation and CA IX by immunochemistry in 22 cases of bladder cancer. The relationship of microvessels to the distribution of CA IX was determined. In a separate series of 49 superficial tumours, CA IX immunostaining was compared with clinico-pathological outcome. In superficial and invasive disease there was overlap in the expression of vascular endothelial growth factor and CA IX, CA IX being more widespread. Both were expressed predominantly on the luminal surface, and surrounding areas of necrosis (invasive tumours). Expression of both factors was greater in superficial disease. Expression was absent within ∼80 μm of microvessels. Unlike vascular endothelial growth factor, CA IX did not predict outcome in superficial disease. Differential responses to reoxygenation provide one explanation: vascular endothelial growth factor mRNA declined rapidly, while CA IX expression was sustained for >72 h. Expression of vascular endothelial growth factor mRNA in bladder tumours is consistent with hypoxic regulation and suggests differential regulation in superficial vs invasive disease. The expression of CA IX on the luminal surface justifies investigation of its utility as a therapeutic target/prognostic indicator
A test of the power law relationship between gamma-ray burst pulse width ratio and energy expected in fireballs or uniform jets
Recently, under the assumption that the Doppler effect of the
relativistically expanding fireball surface is important, Qin et al. showed
that in most cases the power law relationship between the pulse width and
energy of gamma-ray bursts (GRBs)would exist in a certain energy range. We
check this prediction with two GRB samples which contain well identified
pulses. A power law anti-correlation between the full pulse width and energy
and a power law correlation between the pulse width ratio and energy are seen
in the light curves of the majority (around 65%) of bursts of the two samples
within the energy range of BATSE, suggesting that these bursts are likely to
arise from the emission associated with the shocks occurred on a
relativistically expanding fireball surface. For the rest of the bursts, the
relationships between these quantities were not predicted previously. We
propose to consider other spectral evolutionary patterns or other radiation
mechanisms such as a varying synchrotron or Comptonized spectrum to check if
the observed relationships for these rest bursts can also be accounted for by
the Doppler model. In addition, we find that the upper limits of the width
ratio for the two samples do not exceed 0.9, in agrement with what predicted
previously by the Doppler model. The plateau/power law/plateau and the peaked
features predicted and detected previously by Qin et al. are generally
observed, with the exceptions being noticed only in a few cases. According to
the distinct values of two power law indices of FWHM and ratio and energy, we
divide the bursts into three subsets which are located in different areas of
the two indices plane. We suspect that different locations of the two indices
might correspond to different mechanisms.Comment: 16 pages, 7 figures, MNRAS accepte
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