Characterization, cloning and immunogenicity of antigens released by lung-stage larvae of Schistosoma mansoni

Lung-stage schistosomula are the target of protective immunity in mice vaccinated with attenuated cercariae of Schistosoma mansoni. Therefore, proteins present at this developmental stage, and in particular those which are secreted, are a potential source of novel vaccine candidates. However, little information is available about such molecules. Here we describe the cDNA clones identified by screening expression libraries with serum raised against proteins released by lung-stage schistosomula. In total, 11 different cDNA species were identified, 6 of which have been described previously in S. mansoni; these included fructose 1,6-bisphosphate aldolase and Sm21.7 which together accounted for two-thirds of all positive clones. Of the 5 newly described schistosome genes, 1 cDNA had a high degree of homology to the s5a subunit of 26S proteasomes, most significant being with the human protein. The remaining 4 clones showed no significant homologies to any genes sequenced previously. Fructose 1,6-bisphosphate aldolase, Sm21.7, the proteasome homologue and 1 unknown clone (A26) have been expressed in a bacterial expression system and serum produced against each recombinant protein. Immunolocalization showed fructose 1,6-bisphosphate aldolase, Sm21.7 and the proteasome homologue to be most abundant in muscle cells whilst clone A26 was distributed throughout many tissues, but was most abundant in the tegument. Analysis of the cellular immune responses of vaccinated mice showed 3 of the 4 expressed clones to be highly immunogenic, inducing the secretion of large quantities of the Th1-type cytokine interferon gamma

Active clearance control system for a turbomachine

An axial compressor is provided with a cooling air manifold surrounding a portion of the shroud, and means for bleeding air from the compressor to the manifold for selectively flowing it in a modulating manner axially along the outer side of the stator/shroud to cool and shrink it during steady state operating conditions so as to obtain minimum shroud/rotor clearance conditions. Provision is also made to selectively divert the flow of cooling air from the manifold during transient periods of operation so as to alter the thermal growth or shrink rate of the stator/shroud and result in adequate clearance with the compressor rotor

Characterization, cloning and immunogenicity of antigens released by transforming cercariae of Schistosoma mansoni

A schistosome infection is initiated when the parasite penetrates the skin of a susceptible host. Relatively large quantities of protein are released by transforming cercariae compared to later larval stages. This represents the first parasite material to which the host's immune system is exposed, yet little is known about the proteins which are released during the first few hours post-transformation. We have shown that antiserum raised against such molecules was capable of imparting protection against a schistosome challenge infection upon passive transfer to naïve mice. By screening a cercarial cDNA library with this serum, 38 positive clones were identified. Sequence analysis showed these to represent 8 different molecules which included Schistosoma mansoni 21·7 kDa antigen, calcium-binding-protein and the vaccine candidate glutathione S-transferase (Sm28GST). In addition, 5 clones were isolated, 1 of which had significant homology to many cytochrome C proteins, another with leukocyte elastase inhibitors and 3 which represented novel molecules. Four clones were expressed in a prokaryotic high-level expression vector, sera produced against each purified recombinant protein and used subsequently to probe Western blots and parasite sections. The leukocyte elastase inhibitor homologue and 2 unknowns induced significant proliferation by lymph node cells recovered from mice vaccinated with irradiated cercariae. More strikingly, the 2 novel proteins stimulated very high levels of interferon [gamma] (IFN[gamma]) secretion both by lymph node cells and those recovered by broncho-alveolar lavage from the lungs of vaccinated mice. Such results will be discussed in the context of vaccine development

They’re Playing Our Song! The Promise and The Perils of Music Copyright Litigation, 13 J. Marshall Rev. Intell. Prop. L. 555 (2014)

Music copyright cases are unique, costly, difficult, and complex. It was no different in the case where Ray Repp, a music composer for a Catholic publishing house, filed suit against Andrew Lloyd Webber, the famed British composer most famous for such Broadway hits as Cats and Phantom of the Opera. Repp alleged that Webber’s “Phantom Song,” the theme music for the Phantom of the Opera musical, infringed Repp’s song “Till You,” which he wrote and copyrighted almost a decade earlier. Webber in turn claimed that Repp’s “Till You” was in fact a copy of an even earlier Webber song, “Close Every Door.” The entire litigation spanned a decade, generated two trials, four published district court opinions, and a landmark Second Circuit decision. It involved hotly contested issues of venue, testimony of expert musicologists, courtroom waltzes, courtroom demonstrations by Webber and his exwife Sarah Brightman, and media attention. The author, who was lead counsel for Repp and K&R Music, Inc. in the Repp v. Webber litigation, uses the case as a template to illustrate the unique strategies, problems, and promise of music copyright litigation. The article discusses, on an anecdotal basis, useful lessons to be gleaned from the experience and should be studied by any practitioner who wishes to dive into the maelstrom of music copyright litigation

A sensory-guided surgical micro-drill

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2010 The Authors.This article describes a surgical robotic device that is able to discriminate tissue interfaces and other controlling parameters ahead of the drill tip. The advantage in such a surgery is that the tissues at the interfaces can be preserved. A smart tool detects ahead of the tool point and is able to control the interaction with respect to the flexing tissue, to avoid penetration or to control the extent of protrusion with respect to the position of the tissue. For surgical procedures, where precision is required, the tool offers significant benefit. To interpret the drilling conditions and the conditions leading up to breakthrough at a tissue interface, a sensing scheme is used that discriminates between the variety of conditions posed in the drilling environment. The result is a fully autonomous system, which is able to respond to the tissue type, behaviour, and deflection in real-time. The system is also robust in terms of disturbances encountered in the operating theatre. The device is pragmatic. It is intuitive to use, efficient to set up, and uses standard drill bits. The micro-drill, which has been used to prepare cochleostomies in the theatre, was used to remove the bone tissue leaving the endosteal membrane intact. This has enabled the preservation of sterility and the drilling debris to be removed prior to the insertion of the electrode. It is expected that this technique will promote the preservation of hearing and reduce the possibility of complications. The article describes the device (including simulated drill progress and hardware set-up) and the stages leading up to its use in the theatre.Queen Elizabeth Hospital, Birmingham, U

Avoidance of the real and anxiety about the unreal: attachment style and video-gaming

In this article, the authors discuss the light and dark side of attachments and attachment style in physical and digital worlds. They argue that many games offer opportunities for the generation of new and meaningful attachments to both physical and digital others. They discuss two ‘fundamental attachment errors’ and show how these can lead to both ‘light’ outcomes, in terms of opportunities to learn more secure attachment patterns, and ‘dark’ outcomes, where existing dysfunctional behaviours become more pronounced. The authors argue that the avatars which children adopt online have important consequences for their psychosocial development, and that these are mediated through the degree to which the real self is differentiated from the avatar. It is proposed that attachment is a key force in understanding play, and that studying its manifestations and effects in digital playscapes may contribute to understanding the effects of life online, and how insecure attachments may become secure

Use of renewable building materials in residential construction : a review

The development of mass-produced environmentally-benign housing is one of the critical factors in the transition to global sustainability. Such housing will need to be constructed from renewable and/or recycled materials, be conditioned using minimal or no non&mdash;renewable energy, and be affordable. The universal need for such built environment resource stewardship is urgent. In developing countries, the requirement is to shelter growing populations, and in industrialised countries, there is a need for an alternative to the current resource and nergy-intensive material usage in housing. While there are some good surveys of building materials made from renewable resources, such as the BEDP Environment Design Guide Pro 11 by Gelder (2002), there does not appear to be a comprehensive database of these materials linked to abundant and reliable supply. This paper reviews the current availability and potential usage of renewable materials applicable to Australian mainstream residential construction. It concludes that the current state of publicly available information is dispersed and embedded in multiple sources with variance in detail, incomplete access and uncertain comparison across the sources.<br /

Tourism, migration, and the exodus to virtual worlds: place attachment in massively multiplayer online gamers

Place attachment to both physical and virtual places was investigated in an online survey of massively multiplayer online gamers. Participants (N=740) completed a place attachment inventory once for the place in the physical world which they considered home, and once for a place in a virtual world they felt attached to. In addition, measures of personality, gaming motivation, life satisfaction, attachment style, and identification with online avatars were taken. Results suggested that place identity, place uniqueness, and place social bonding were higher for physical places than for virtual places, but that place affect was higher for virtual places. A small number of participants (N=55, 7%) identified virtual ‘homes’, which participants felt were more special and which they identified more strongly with than other virtual places, and that were as unique and associated with an equal sense of belonging to physical homes. Results are interpreted through the lens of migration theory, and recommendations made for future research into digital domiciles and migration