28 research outputs found
Autocuidado e prevenção do câncer de mama entre mulheres costureiras
Conhecer as percepções sobre o autocuidado e prevenção do câncer de mama entre mulheres costureiras. Estudo descritivo, transversal de abordagem qualitativa, com a participação de 15 mulheres selecionadas por conveniência, no mês de abril de 2021. A análise dos dados utilizou o módulo de processamento de Classificação Hierárquica Descendente (CHD). Destaca-se a importância do enfermeiro na APS com ações de prevenção do câncer de mama, com 14,81% e 22,22% o que oportuniza um maior entendimento do assunto por parte das mulheres. Bem como é sabido pela maior parte das entrevistadas com estimativa de 18,52% e 25,93% que a herança genética pode ser determinante no acometimento dessa patologia. O autocuidado e a prevenção do câncer de mama em mulheres ainda são escassas pelo fato de que existe algumas dificuldades por parte dos profissionais de saúde em fazer o rastreamento principalmente do público costureiras, a prática do autocuidado e o pouco conhecimento com relação a predisposição genética como fator de risco para desenvolvimento do câncer de mama
Análise da aplicabilidade do protocolo de identificação correta do paciente: Um relato de experiência / Analysis of applicability correct patient identification protocol: An experience report
Objetivo: Analisar o protocolo de identificação do paciente e sua aplicabilidade dentro do serviço de saúde, correlacionando à teoria e prática observada com as orientações do ministério da saúde, ANVISA e Fiocruz. Método: Trata-se de um estudo descritivo, com abordagem qualitativa, do tipo relato de experiência, fundamentado na análise do protocolo de identificação do paciente utilizado pela instituição hospitalar, e os protocolos normativos dos órgãos de saúde. Resultados: Foi analisado o protocolo de identificação do paciente e diante percebe-se que a instituição segue em sua grande maioria de acordo com o que os protocolos de saúde determinam. Considerações finais: Conclui-se que embora o protocolo existente no serviço esteja embasado no Protocolo de Identificação do Paciente, ainda sim existe uma necessidade de sua atualização e a realização de educação permanente para os profissionais que realizam a assistência
Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family
Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. in an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. the monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. the monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. the assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)ERC AdG SISYPHEUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Metab Macromol Firmino Torres de Castro, BR-21941 Rio de Janeiro, BrazilLab Bioinformat, Lab Nacl Computacao Cient, Rio de Janeiro, BrazilINRIA Grenoble Rhone Alpes, BAMBOO Team, Villeurbanne, FranceUniv Lyon 1, CNRS, UMR5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, FranceUniv Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, São Paulo, BrazilUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, São Paulo, BrazilLab Nacl Ciencia & Tecnol Bioetano, São Paulo, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, BrazilUniv Fed Goias, Inst Ciencias Biol, Mol Biol Lab, Goiania, Go, BrazilFundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Biol Mol Tripanossomatideos, Curitiba, Parana, BrazilFundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Genom Func, Curitiba, Parana, BrazilUniv Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, São Paulo, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, BrazilUniv Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, Ctr Ciencias Biol, Lab Protozool & Bioinformat, Florianopolis, SC, BrazilUniv Fed Vicosa, Dept Bioquim & Biol Mol, Ctr Ciencias Biol & Saude, Vicosa, MG, BrazilInst Butantan, Lab Especial Ciclo Celular, São Paulo, BrazilUniv São Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Scienc
Gender, Masculinities and Men's Health: development of a curricular discipline in the undergraduate nursing course
Objective: to describe the development of a curricular discipline in the undergraduate nursing course in the area of ​​gender, masculinities and men's health. Method: A descriptive, qualitative study, originating from teaching curricular activity in the undergraduate nursing course of a Higher Education Institution in a municipality in Bahia, Brazil, between the years 2014 to 2020. Sources of institutional data, imagery records, teaching-learning activities produced in the component and teachers' reports. The apprehended data were themed from the content analysis proposed by and interpreted in the light of the masculinity framework from the perspective of Raewyn Connell. Results: The development of the curricular component came from academic dialogues in line with the educational institution, under the convergence with the demands and needs of students and for nursing education. We sought to meet the initiatives to strengthen the implementation of the National Policy for Integral Attention to Men's Health of the Brazilian Ministry of Health, such as the epidemiological indicators and nursing education under the relational perspective of gender and masculinities in the production of health care. health directed at the male audience. Conclusion: The development of the curricular component provided an opportunity for the expansion and strengthening of qualified nursing training, the contribution to overcoming the invisibility of the male audience in actions and in health care, promoted the integration of teaching and service from the academic extension and fostered scientific production directed to Nursing in men's health.</p
Performance of a qualitative rapid chromatographic immunoassay to diagnose COVID-19 in patients in a middle-income country
Numbers of ORFs identified in <i>A. deanei</i> and <i>S. culicis</i> and their symbionts, according to the mechanisms of DNA replication and repair, signal transduction, transcription and translation.
<p>Numbers of ORFs identified in <i>A. deanei</i> and <i>S. culicis</i> and their symbionts, according to the mechanisms of DNA replication and repair, signal transduction, transcription and translation.</p
Members of the Fts family and PBPs that are present in endosymbionts of <i>A. deanei</i> and <i>S. culicis</i>.
<p>nd: not determined.</p
Protein Reference Sequence-Guided Assembly data of <i>A. deanei</i> and <i>S. culicis</i> genomes.
<p>Protein Reference Sequence-Guided Assembly data of <i>A. deanei</i> and <i>S. culicis</i> genomes.</p
Phylogenetic of histones of <i>A. deanei, S. culicis,</i> and other trypanosomatids.
<p>Histone protein (panel A) and nucleotide (panel B) sequences were generated by MUSCLE tool using 10 iterations in the Geneious package <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060209#pone.0060209-Drummond1" target="_blank">[120]</a>. Trees were constructed using the Geneious Tree Builder, by employing Jukes-Cantor genetic distance model with a neighbor-joining method and no out-groups. The consensus trees were generated from 100 bootstrap replicates of all detected histone genes, as shown below. Scale bars are indicated for each consensus tree. The trees in panel A are based in a collection of sequences of all trypanosomatids. The nucleotide sequences used for dihydrofolate reductase-thymidylate synthase are: <i>T. cruzi,</i> XM_810234; <i>T. brucei</i>, XM_841078; <i>T. vivax,</i> HE573023; <i>L. mexicana</i>, FR799559; <i>L. major</i>, XM_001680805; <i>L. infantum</i>, XM_001680805; and <i>C. fasciculata</i>, M22852.</p
Summary of the origin of ORFs found in <i>A. deanei</i> and <i>S. culicis.</i>
*<p>Number of genes with identity to Prokaryotes.</p>**<p>Number of genes with identity to Eukaryotes.</p>***<p>Ratio of the number of genes with identity to Prokaryotes/Eukaryotes.</p