Izolacija emergentnih ljudskih patogena i patogena koji se prenose hranom u kliničkim slučajevima infekcija od pasa i mačaka zaprimljenih u veterinarsku kliniku u sjevernom Portugalu

Nowadays, dogs and cats are considered family members, translating into increased proximity to humans. Studies have shown that 40-60% of owners have very close interactions e.g., sharing the same dish, face lickings, or sleeping on the same bed. This promotes the exchange of microorganisms between species. Therefore, an One Health approach should be applied to encompass both human and animal health problems. In this study, 37 cases of companion animals (54.1% dogs and 45.9% cats) in a veterinary clinic in northern Portugal were analysed, and urinary and eye infections, respiratory conditions, and dermatological problems were confirmed. According to sample type, 43.2% were from urine, 13.5% from ear exudate, 8.1% from skin exudate, 8.1% from tracheal exudate, 2.7% from vaginal exudate, 2.7% from conjunctiva exudate, 2.7% from purulent exudate, 2.7% from bronchoalveolar exudate, 2.7% from ocular exudate, and 2.7% from blood. In all, 21 pathogen species were isolated, of which Pantoea agglomerans, Cronobacter sakazakii, Leclercia adecarboxylata, Sphingomonas paucimobilis are currently considered emerging agents of human infection. The occurrence of these species was 2.4% for Leclercia adecarboxylata (one young male cat), 2.4% for Pantoea agglomerans (one adult male dog), 2.4% for Cronobacter sakazakii (one adult male dog), and 2.4% for Sphingomonas paucimobilis (one adult male dog). Leclercia adecarboxylata is a gram-negative bacillus of the Enterobacteriaceae family. It is a ‘novel’ rare human pathogen, mostly affecting immunocompromised individuals or causing polymicrobial infections in immunocompetent patients. Cronobacter sakazakii is an emerging foodborne pathogen that causes necrotizing enterocolitis and bacteremia in humans, Pantoea agglomerans is associated with a hospital-acquired infection, mostly in immunocompromised individuals with a fatal outcome. Sphingomonas paucimobilis is an emerging opportunistic bacterium with a particular tropism toward bones and soft tissues. The intimate relationship between humans and companion animals presents a potential risk for the transmission of zoonotic pathogens. Therefore, more research based on an One Health approach should be performed to more accurately determine the occurrence and incidence of emerging agents between species.U današnje vrijeme, psi i mačke smatraju se članovima obitelji, što podrazumijeva sve veću bliskost s ljudima. Nekoliko studija pokazalo je da 40-60 % vlasnika ima vrlo blizak odnos, npr. dijeljenje iste hrane, lizanje lica ili spavanje na istom krevetu. To potiče razmjenu mikroorganizama među vrstama. Iz tog je razloga potrebno primijeniti pristup Jednog zdravlja da bi se obuhvatili i ljudski i životinjski zdravstveni problemi. U ovoj je studiji analizirano 37 slučajeva kućnih ljubimaca (54,1 % pasa i 45,9 % mačaka) u veterinarskoj klinici u sjevernom Portugalu. Kazuistika je uključivala infekcije urinarnog trakta i očiju, respiratorna stanja i dermatološke probleme. Prema vrsti uzoraka, 43,2 % su bili iz urina, 13,5 % iz eksudata iz uha, 8,1 % iz eksudata iz kože, 8,1 % iz eksudata iz dušnika, 2,7 % iz vaginalnog eksudata, 2,7 % iz eksudata iz sluznice, 2,7 % iz gnojnog eksudata, 2,7 % iz bronhoalveolarnog eksudata, 2,7 % iz očnog eksudata i 2,7 % iz krvi. Izolirani su patogeni 21 različite vrste, od čega se Pantoea agglomerans, Cronobacter sakazakii, Leclercia adecarboxylata, Sphingomonas paucimobilis, trenutno smatraju emergentnim uzročnicima ljudske infekcije. Pojavnost ovih vrsta bila je 2,4 % za Leclercia adecarboxylata (jedan mladi mačak), 2,4 % za Pantoea agglomerans (jedan mladi pas), 2,4 % za Cronobacter sakazakii (jedan mladi pas) i 2,4 % za Sphingomonas paucimobilis (jedan mladi pas). Leclercia adecarboxylata je gram-negativni bacil iz obitelji enterobakterija. To je rijedak „novi“ ljudski patogen koji uglavnom pogađa imunokompromitirane pojedince ili prouzroči polimikrobne infekcije u imunokompetentnih bolesnika. Cronobacter sakazakii je emergentni patogen koji se prenosi hranom, a koji prouzroči nekrotizirajući enterokolitis i bakterijemiju u ljudi, Pantoea agglomerans povezana je s bolničkom infekcijom, uglavnom kod imunokompromitiranih pojedinaca sa smrtnim slučajevima. Sphingomonas paucimobilis je emergentna oportunistička bakterija s posebnim tropizmom za kosti i meka tkiva. Bliski odnos između ljudi i ljubimaca predstavlja potencijalni rizik prijenosa zoonotskih patogena. Iz tog je razloga potrebno provesti dodatna istraživanja na temelju pristupa Jednog zdravlja, da bi se preciznije odredila pojavnost i incidencija emergentnih uzročnika među vrstama

Maspin, p63 e bcl2 in odontogenic keratocyst tumor, dentigerous cyst and ameloblastoma

Os cistos e tumores odontogênicos sempre tiveram grande importância dentro da Odontologia, seja pela grande prevalência clínica seja pelo grande acometimento do indivíduo afetado pela lesão. A nova classificação da Organização Mundial de Saúde trouxe a mudança de categoria do queratocisto, que recebe agora a nômina de tumor odontogênico queratocístico, e que figura não mais na categoria de cisto odontogênico de desenvolvimento, mas sim de tumor odontogênico. Certa precipitação nessa mudança levou alguns autores a sugerirem a necessidade de estudos que esclareçam as características clínicas e histopatológicas dessa lesão para que se tenha uma classificação realmente precisa. O grande paradigma dessa lesão é: ao mesmo tempo em que apresenta características histológicas de um cisto, possui um comportamento clínico agressivo mais comumente observado nos tumores. O que realmente difere esta lesão das outras lesões que se inseriam no mesmo grupo é o padrão de crescimento diferenciado do tumor odontogênico queratocístico em relação às outras lesões císticas. Sendo assim, poderia se suspeitar que essa lesão possua um potencial proliferativo maior do que as outras que anteriormente pertenciam ao mesmo grupo, denotando uma regulação diferenciada do mecanismo proliferação-apoptose. Este estudo teve como objetivo comparar o tumor odontogênico queratocístico com uma lesão cística - o cisto dentígero - e com uma lesão tumoral - o ameloblastoma ? por meio de marcadores imunoistoquímicos para supressão tumoral e anti-apoptose. Os resultados demostraram que a maior diferença entre essas lesões está principalmente na atividade apoptótica, já que somente o resultado de bcl2 foi estatisticamente significante entre essas lesões.Odontogenic cysts and tumors have always had a great importance in Dentistry, for its high clinical prevail and for its noticeable invasive behavior. The new classification released by WHO rearranged keratocyst, that is named now odontogenic keratocystic tumor, classifying it no longer as a development cyst, but now as odontogenic tumor. Certain haste in this change brought some authors to suggest the necessity of more studies to clarify the feautures of such lesions to determine a more accurate classification. The greatest paradigm of this lesion is that it shows cyst-like histological characteristics and simultaneuosly it has an aggressive clinical behavior, which is more commonly observed in tumors. The main difference between this lesion and the others cystic lesions is the growth pattern, which suggests that the odontogenic keratocystic tumor has higher proliferative potential than other cystic lesions. The aim of this research was to compare odontogenic keratocystic tumor with a cystic lesion ? dentigerous cyst ? and with a tumoral lesion ? ameloblastoma ? using tumor suppressor and anti-apoptosis immunohistochemical expression. Results show that the more important difference among the analysed lesions is apoptosis activity, since only bcl2 staining was significantly different among them

Study of TP53 gene mutation and immunohistochemical analysis of p53, Bcl2 e Fas expression in actinic cheilitis and lip squamous cell carcinoma

A radiação ultravioleta ao atingir os seres humanos em grande quantidade e durante uma longa exposição pode provocar danos específicos ao DNA, sendo causa de várias lesões como o carcinoma epidermoide de lábio e a queilite actínica, que é considerada uma lesão precedente ao aparecimento da primeira. Entre os danos causados pela radiação UV está a alteração genética do TP53, provocando anomalias na proteína por ele codificada. A produção da proteína p53 somente é recrutada em situações de estresse como: radiação ionizante, hipoxia ou ativação de oncogenes, nas quais sua função é regular o ciclo celular e ativar vias de apoptose. Contudo, sabe-se que no processo de carcinogênese não somente as alterações não reparadas do DNA são responsáveis pelo aparecimento de uma lesão. Outro fator de extrema importância nesse processo são os mecanismos de apoptose, entre os quais estão as vias do Bcl2 e do Fas. A queilite actínica normalmente é classificada segundo seus graus de displasia, o que para alguns sugeriria os passos percorridos por essa lesão até o carcinoma epidermoide de lábio já que esta lesão possui um potencial de malignização. Os resultados deste trabalho demonstraram que não há diferença estatística na expressão gênica e imuno-histoquímica de p53 entre os diversos graus de displasia. Demonstraram ainda que as vias de apoptose de Bcl2 e Fas estavam ocorrendo normalmente. Sugere-se então que não há comprovação de que ocorra uma progressão para a malignização passando por todos os graus de displasia da queilite actínica tendo, todos a mesma possibilidade de se transformarem em um carcinoma epidermoide de lábio.The long exposition of the human tissues to the ultraviolet radiation causes DNA damages and consequently several lesions might appear. Among them, lip squamous cell carcinoma and actinic cheilitis which is considered to be a condition that precedes squamous cell carcinomas. The TP53 mutation is a well-known effect of UV radiation, leading to the synthesis of an anomalous protein. The p53 production is only recruit in stress conditions like: ionizing radiation, hypoxia or oncogene activation, where it plays a role in cell cycle regulation and apoptosis activation. Apoptosis is also a fundamental mechanism associated to carcinogenesis. Bcl2 e Fas pathways are central in the regulation of this process. It is usual to classify the epithelial dysplasia degree in actinic cheilitis as a prognosticator. This type of classification suggests a one way path towards malignization. Our results have shown that there is no statistical difference in TP53 status and p53 expression among the different degrees of actinic cheilitis epithelial dysplasia and squamous cell carcinoma. Based on the findings, there is no prove that the malignant transformation occurs as the epithelial dysplasia progresses. Therefore, should be considered that any degree has the same probability to become a lip squamous cell carcinom

Comparison of the effects of shock waves versus radiofrequency on abdominal lipolysis: A randomized clinical trial

High levels of abdominal adiposity mean higher risk of developing cardiovascular diseases. Aerobic exercise per si reduces the risk of developing this type of diseases. Radiofrequency and shockwave therapy showed to be effective in the reduction of localized abdominal fat. Randomized clinical trial, with 30 volunteers in fertile age with overweight and/or obese, randomly and equitably allocated in two experimental groups (1—shockwave therapy and 2—radiofrequency) and one control group. Aerobic physical exercise was prescribed to all groups. The values of anthropometric measurements were measured in two moments. The intervention protocol was performed in a clinic for six weeks, with one session per week. The ANOVA test, paired samples t test, Kruskal-Wallis test, and Wilcoxon test were used to compare the results, for a significance level of 0.05. Of 28 participants completed the study. There were significant differences in waist circumference reduction between the experimental group 1 and 2 when compared to the control group (p = 0.005 and p = 0.014, respectively). There was a statistically significant reduction in navel level circumference in experimental group 1 when compared to experimental group 2 (p = 0.024) and with the control group (p = 0.016). Both resources were effective in reducing abdominal measurements when compared to the control group. However, SWT was superior in reducing the navel level circumference.info:eu-repo/semantics/publishedVersio

Clinicopathologic and immunohistochemical features of oral neurofibroma

Objective. The purposes of this study were to assess clinical, histopathological and immunohistochemical features of 22 oral neurofibromas (NFs) and discuss with previously described literature, addressing the main aspects regarding the differential diagnosis. Materials and methods. Immunohistochemical reactions included S-100, CD34, GLUT-1, EMA, Ki-67, p53 and Collagen IV and histochemical reactions for Alcian blue. Results. Clinically, the preferential location was the maxillary bones, tongue and buccal mucosa. Microscopically, widely spread spindle-shaped cells with scant cytoplasm and elongated nuclei were observed. Immunostaining revealed that the tumor cells weakly expressed GLUT-1, Collagen IV, Ki-67 and p53. They were variably positive for CD34, S-100 protein and membrane epithelial antigen (EMA). Conclusions. The different types of nerve sheath cells observed in the present series reinforce the presence of heterogeneous population in NFs. The strong positivity for S-100 suggests that the lesions were more composed by S-100-positive Schwann cells than other cells. Besides, the high number of CD34-positive cells suggests that this marker can be useful for the differential diagnosis of NFs against PEN, traumatic neuromas and Schwannomas. Finally, the low immunostaining for p53 and Ki-67 may indicate that NFs massively composed by S-100-positive Schwann cells present low potential of aggressiveness and malignant transformation

Perceção dos residentes face ao impacto do turismo

In tourism the local community are not only one of the most essential element for the destination offer but also the key for its differentiation. The knowledge about their expectation and satisfaction regarding tourism development is extremely important for the development process itself. Based on that statement it was developed an empirical research where the local community perception emerges as the key element to be considered. Specifically, in this project it was analysed the perception of the local community, in two different small Portuguese villages, regarding the development of tourism as well as their position regarding the side effects that a possible increase in tourism could provide. It was concluded the positive relationship between the perception of the local image and the perception of economic and socio-cultural impacts caused by tourism and an inverse relationship between the perception of the local image and the perception of environmental caused by tourism.info:eu-repo/semantics/publishedVersio

Touristic experiences the living in the Grandma’s house’ project

The goal of this research is focused on the project “Living in the Grandma's House” which aims to contribute to the fight against desertification of rural areas and to create unique touristic experiences in rural areas of the Inland North of Portugal. After an ethnographic study, two villages in Trás-os-Montes region were identified in which "Grandma’s" with interest and aptitude to receive visitors through a prior sale and booking (through the website and application of the developed project), were selected to deliver a unique experience to visitors: listen to stories, legends, while sharing a meal in a private house together with the owner which will strengthen the contact between the visitor and the reality visited. It is considered that the effective value of this research is based on the modelling of a feasible and entrepreneurial concept, which is grounded on the particularities of a low-density territory, whose subsequent replication will result in very positive results for the local communities. The methodology adopted is founded on a qualitative approach, which begins with a bibliographical review about the concept of touristic experiences and is based on the analysis of the project “Living in the Grandma's House”.O presente trabalho foi desenvolvido no âmbito do Projeto Viv@vó: “Viver na Casa da Avó”, com a referência NORTE-01-0145-FEDER-023637, financiado pelo Programa Operacional Regional do Norte, Aviso 02/SAICT/2016.info:eu-repo/semantics/publishedVersio

Sistema aplicado em moldagem de elementos para drenagem urbana e elementos pré-moldados

Universidade Federal do Rio Grande do SulEngenhariaDepositad

Assessment of calcinosis in Portuguese patients with systemic sclerosis: a multicenter study

© The Author(s), under exclusive licence to International League of Associations for Rheumatology (ILAR) 2023Introduction/objectives: The study aims to define the clinical and subclinical calcinosis prevalence, the sensitivity of radiographed site and clinical method for its diagnosis, and the phenotype of Portuguese systemic sclerosis (SSc) patients with calcinosis. Method: A cross-sectional multicenter study was conducted with SSc patients fulfilling Leroy/Medsger 2001 or ACR/EULAR 2013 classification criteria, registered in the Reuma.pt. Calcinosis was assessed through clinical examination and radiographs of hands, elbows, knees, and feet. Independent parametric or non-parametric tests, multivariate logistic regression, and sensitivity calculation of radiographed site and clinical method for calcinosis detection were performed. Results: We included 226 patients. Clinical calcinosis was described in 63 (28.1%) and radiological calcinosis in 91 (40.3%) patients, of which 37 (40.7%) were subclinical. The most sensitive location to detect calcinosis was the hand (74.7%). Sensitivity of the clinical method was 58.2%. Calcinosis patients were more often female (p = 0.008) and older (p < 0.001) and had more frequently longer disease duration (p < 0.001), limited SSc (p = 0.017), telangiectasia (p = 0.039), digital ulcers (p = 0.001), esophageal (p < 0.001) and intestinal (p = 0.003) involvements, osteoporosis (p = 0.028), and late capillaroscopic pattern (p < 0.001). In multivariate analysis, digital ulcers (OR 2.63, 95% CI 1.02-6.78, p = 0.045) predicted overall calcinosis, esophageal involvement (OR 3.52, 95% CI 1.28-9.67, p = 0.015) and osteoporosis (OR 4.1, 95% CI 1.2-14.2, p = 0.027) predicted hand calcinosis, and late capillaroscopic pattern (OR 7.6, 95% CI 1.7-34.9, p = 0.009) predicted knee calcinosis. Anti-nuclear antibody positivity was associated with less knee calcinosis (OR 0.021, 95% CI 0.001-0477, p = 0.015). Conclusions: Subclinical calcinosis high prevalence suggests that calcinosis is underdiagnosed and radiographic screening might be relevant. Multifactorial pathogenesis may explain calcinosis predictors' variability. Key Points • Prevalence of subclinical calcinosis in SSc patients is substantial. • Hand radiographs are more sensitive to detect calcinosis than other locations or clinical method. • Digital ulcers were associated with overall calcinosis, esophageal involvement and osteoporosis were associated with hand calcinosis, and late sclerodermic pattern in nailfold capillaroscopy was associated with knee calcinosis. • Anti-nuclear antibody positivity may be a protective factor for knee calcinosis.info:eu-repo/semantics/publishedVersio