Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase

β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction conditions affecting the specific selectivity of A. oryzae β-galactosidase for synthesis of OsLu has been carried out to enhance OsLu with DP ≥ 3 production. Assays with β-galactosidase immobilized onto a glutaraldehyde–agarose support were also carried out with the aim of making the process cost-effective and industrially viable. Optimal conditions with both soluble and immobilized enzyme for the synthesis of OsLu with DP ≥ 3 were 50 °C, pH 6.5, 450 g/L of lactulose, and 8 U/mL of enzyme, reaching yields of ca. 50% (w/v) of total OsLu and ca. 20% (w/v) of OsLu with DP 3, being 6′-galactosyl-lactulose the major one, after a short reaction time. Selective formation of disaccharides, however, was favored at 60 °C, pH 4.5, 450 g/L of lactulose and 8 U/mL of enzyme. Immobilization increased the enzymatic stability to temperature changes and allowed to reuse the enzyme. We can conclude that the use, under determined optimal conditions, of the A. oryzae β-galactosidase immobilized on a support of glutaraldehyde–agarose constitutes an efficient and cost-effective alternative to the use of soluble β-galactosidases for the synthesis of prebiotic OsLu mixturesThis work has been supported by projects AGL2014-53445-R from Ministerio de Economía y Competitividad; ALIBIRD-CM S-2013/ABI-2728 from Comunidad Autónoma de Madrid and COOPB20099 from CSI

Development of a prototype for submarine communications in shallow waters

The Telecommunications Marine Laboratory (TML) of the University of Cádiz takes part in a research project to study the intertidal sediments of the Bay of Cádiz. The collection of samples from the seabed and the transmission of data to the surface are performed by a Mini Profiler with four channels (MP4), which is supplied by UNISENSE. Each channel processes the samples collected by a single sensor. For power, control, and data transmission, a special wiring that connects the submerged subsystem with the surface subsystem is used. The tension transmitted by the cable to the MP4 structure makes its handling and positioning under water more difficult. This article explains the technological solution developed by the TML to replace some wiring from the MP4. For the prototype, two underwater acoustic modems have been used and an electronic system has been developed based on the Arduino platform, in order to multiplex, digitize, and transmit the data collected by the MP4 sensors, eliminating the need to use wiring. The submerged subsystem has an autonomous power supply by a battery.Peer Reviewe

Agricultural Reforms, Land Distribution, and Non-Sugar Agricultural Production in Cuba

Since 2007, the Cuban government has introduced a series of agricultural reforms to increase non-sugar agricultural production and reduce the country’s dependency on food and agricultural imports. The most important agricultural reforms implemented in Cuba (so far) include: (a) increases in the prices paid by the state for selected agricultural products, (b) restructuring the Ministry of Agriculture (MINAGRI) and the Ministry of the Sugar Industry (MINAZ), (c) a new agricultural tax system, (d) the authorisation of direct sales and commercialisation of selected agricultural products, (e) micro-credits extended by stateowned banks to private farmers and usufructuaries, and (f) the expansion of usufruct farming. These reforms have contributed to the redistribution of Cuba’s agricultural land from the state to the non-state sector, notable reductions in idle (non-productive) agricultural land, and mixed results in terms of agricultural output. However, they have not been able to sufficiently incentivise output and reduce the country’s high dependency on agricultural and food imports to satisfy the needs of its population. Achieving these long-desired objectives requires the implementation of more profound structural reforms in this vital sector of the Cuban economy