“We Became Rich and We Lost Everything”: Ethnobotany of Remote Mountain Villages of Abruzzo and Molise, Central Italy

Profound socioeconomic changes affected mountains of Central Italy during the last century and many traditional agro-pastoral activities were abandoned. A few ethnobotanical studies in this area have specifically documented local wild plants used decades ago, but without analyzing in-depth how and why Traditional Ecological Knowledge (TEK) has eroded or changed over time. In this study, we 1) document ethnobotanical uses of four high-altitude remote villages of Central Italy, 2) discuss how these uses have changed over time, comparing them with fieldwork that was conducted 40 years earlier; and 3) assess how plant uses have changed across space, particularly whether the remoteness of villages or the occurrence of Sacred Natural Sites (SNS) have affected TEK linked to wild plants. Sixty semi-structured interviews revealed the use of 83 taxa belonging to 35 families. We did not find any relationship between SNS and the richness of TEK, as these SNS were not inhabited by monastic communities that could have shared their scholarly knowledge. There was not a relationship between remoteness and richness of TEK. The common statement emerging from the field, “We became rich and lost everything”, revealed how socio-economic changes resulted in the rapid abandonment of traditional practices, while the ubiquity of pharmacies may have contributed to the erosion of ethnomedicinal knowledge

Scholarly vs. Traditional Knowledge: Effects of Sacred Natural Sites on Ethnobotanical Practices in Tuscany, Central Italy

Sacred Natural Sites (SNSs), found in all inhabited continents, are cultural landscapes of spiritual significance for local communities. As they are believed to influence Traditional Ecological Knowledge (TEK), we documented the use of wild and semi-domesticated plants for food and medicine in four villages located at different distances from SNSs in Central Italy. Results may indicate that SNSs, which have been managed and inhabited for centuries by monastic communities, have had a restrictive impact on local TEK, as the communities located near SNSs reported fewer traditional uses for plants than those living further from the same SNSs. One possible explanation is that the Scholarly Knowledge (SK) held by the monastic communities of SNSs competed with the TEK of the surrounding villages and this resulted in a smaller body of plant-related folk knowledge, practices and beliefs retained by the people living in the vicinity of SNSs. Further studies should address the past and current mechanisms of competition and/or osmosis between TEK and SK in terms of both daily practices and beliefs/theoretical knowledge

Blended divergences: local food and medicinal plant uses among Arbëreshë, Occitans, and autochthonous Calabrians living in Calabria, Southern Italy

A study focusing on traditional uses of wild plants for gastronomic and medicinal purposes was carried out among three linguistic communities in Calabria, southern Italy. Ninety interviews with local elderly informants were conducted among Occitans and Arbëreshë, two linguistic minorities, and the dominant culture of autochthonous Calabrians. We recorded 85 taxa belonging to 39 botanical families and 66 different detailed use-reports including 35 culinary and 31 medicinal uses. Our overall data show the permanence of traditional ecological knowledge related to wild and semi-domesticated food and medicinal plants; however, high similarity indices among the three communities demonstrate that traditional ecological knowledge is following the pathway of homogenization and standardization toward the dominant culture, facilitated by a context of linguistic erosion, limited intergenerational transmission, and a centuries-old diffusion with Calabrian culture. Moreover, our study calls for further field surveys in isolated areas of Calabria to analyze how traditional ecological practices can be key tools in the development of local small-scale economies through the promotion of artisanal food entrepreneurship of wild food plant transformation

Many-core applications to online track reconstruction in HEP experiments

Interest in parallel architectures applied to real time selections is growing in High Energy Physics (HEP) experiments. In this paper we describe performance measurements of Graphic Processing Units (GPUs) and Intel Many Integrated Core architecture (MIC) when applied to a typical HEP online task: the selection of events based on the trajectories of charged particles. We use as benchmark a scaled-up version of the algorithm used at CDF experiment at Tevatron for online track reconstruction - the SVT algorithm - as a realistic test-case for low-latency trigger systems using new computing architectures for LHC experiment. We examine the complexity/performance trade-off in porting existing serial algorithms to many-core devices. Measurements of both data processing and data transfer latency are shown, considering different I/O strategies to/from the parallel devices.Comment: Proceedings for the 20th International Conference on Computing in High Energy and Nuclear Physics (CHEP); missing acks adde

Prophylactic Use of Liposomal Amphotericin B in Preventing Fungal Infections Early After Liver Transplantation: a Retrospective, Single-Center Study

In this study the authors evaluated the efficacy of prophylaxis with liposomal amphotericin B (L-AmB) in the incidence of fungal infections (FI) during the first 3 months after liver transplant (LT). The study was retrospective and accessed a 4-year period from 2008 to 2011. All patients who died in the first 48 hours after LT were excluded. Patients were divided by the risk groups for FI: Group 1, high-risk (at least 1 of the following conditions: urgent LT; serum creatinine >2 mg/dL; early acute kidney injury [AKI] after LT; retransplantation; surgical exploration early post-LT; transfused cellular blood components [>40 U]); and Group 2, low-risk patients. Group 1 patients were further separated into those who received antifungal prophylaxis with L-AmB and those who did not. Prophylaxis with L-AmB consisted of intravenous administration of L-AmB, 100 mg daily for 14 days. Four hundred ninety-two patients underwent LT; 31 died in the first 48 hours after LT. From the remaining 461 patients, 104 presented with high-risk factors for FI (Group 1); of these, 66 patients received antifungal prophylaxis and 38 did not. In this group 8 FI were observed, 5 in patients without antifungal prophylaxis (P = .011). Three more FI were identified in Group 2. By logistic regression analysis, the categorical variable high-risk group was independently related to the occurrence of invasive FI (P = .006). We conclude that prophylaxis with L-AmB after LT was effective in reducing the incidence of FI. No influence on mortality was detected

Genome-Wide DNA Methylation and Gene Expression Profiles in Cows Subjected to Different Stress Level as Assessed by Cortisol in Milk

Dairy cattle health, wellbeing and productivity are deeply affected by stress. Its influence on metabolism and immune response is well known, but the underlying epigenetic mechanisms require further investigation. In this study, we compared DNA methylation and gene expression signatures between two dairy cattle populations falling in the high- and low-variant tails of the distribution of milk cortisol concentration (MC), a neuroendocrine marker of stress in dairy cows. Reduced Representation Bisulfite Sequencing was used to obtain a methylation map from blood samples of these animals. The high and low groups exhibited similar amounts of methylated CpGs, while we found differences among non-CpG sites. Significant methylation changes were detected in 248 genes. We also identified significant fold differences in the expression of 324 genes. KEGG and Gene Ontology (GO) analysis showed that genes of both groups act together in several pathways, such as nervous system activity, immune regulatory functions and glucocorticoid metabolism. These preliminary results suggest that, in livestock, cortisol secretion could act as a trigger for epigenetic regulation and that peripheral changes in methylation can provide an insight into central nervous system functions

Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles

The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.info:eu-repo/semantics/publishedVersio

Geographical contrasts of Y-chromosomal haplogroups from wild and domestic goats reveal ancient migrations and recent introgressions

By their paternal transmission, Y-chromosomal haplotypes are sensitive markers of population history and male-mediated introgression. Previous studies identified biallelic single-nucleotide variants in the SRY, ZFY and DDX3Y genes, which in domestic goats identified four major Y-chromosomal haplotypes, Y1A, Y1B, Y2A and Y2B, with a marked geographical partitioning. Here, we extracted goat Y-chromosomal variants from whole-genome sequences of 386 domestic goats (75 breeds) and seven wild goat species, which were generated by the VarGoats goat genome project. Phylogenetic analyses indicated domestic haplogroups corresponding to Y1B, Y2A and Y2B, respectively, whereas Y1A is split into Y1AA and Y1AB. All five haplogroups were detected in 26 ancient DNA samples from southeast Europe or Asia. Haplotypes from present-day bezoars are not shared with domestic goats and are attached to deep nodes of the trees and networks. Haplogroup distributions for 186 domestic breeds indicate ancient paternal population bottlenecks and expansions during migrations into northern Europe, eastern and southern Asia, and Africa south of the Sahara. In addition, sharing of haplogroups indicates male-mediated introgressions, most notably an early gene flow from Asian goats into Madagascar and the crossbreeding that in the 19th century resulted in the popular Boer and Anglo-Nubian breeds. More recent introgressions are those from European goats into the native Korean goat population and from Boer goat into Uganda, Kenya, Tanzania, Malawi and Zimbabwe. This study illustrates the power of the Y-chromosomal variants for reconstructing the history of domestic species with a wide geographical range