The StEllar Counterparts of COmpact high velocity clouds (SECCO) survey. II. Sensitivity of the survey and an Atlas of Synthetic Dwarf Galaxies

SECCO is a survey devoted to the search for stellar counterparts within Ultra Compact High Velocity Clouds. In this contribution we present the results of a set of simulations aimed at the quantitative estimate of the sensitivity of the survey as a function of the total luminosity, size and distance of the stellar systems we are looking for. For all our synthetic galaxies we assumed an exponential surface brightness profile and an old and metal-poor population. The synthetic galaxies are simulated both on the images and on the photometric catalogs, taking into account all the observational effects. In the fields where the available observational material is of the top quality we detect synthetic galaxies as >=5 sigma over-densities of resolved stars down to muV,h=30.0 mag/arcsec2, for D<=1.5 Mpc, and down to muV,h~29.5 mag/arcsec2, for D<=2.5 Mpc. In the field with the worst observational material of the whole survey we detect synthetic galaxies with muV,h<=28.8 mag/arcsec2 out to D<=1.0 Mpc, and those with muV,h<=27.5 mag/arcsec2 out to D<=2.5 Mpc. Dwarf galaxies with MV=-10, with sizes in the range spanned by known dwarfs, are detected by visual inspection of the images up to D=5 Mpc independently of the image quality. In the best quality images dwarfs are partially resolved into stars up to D=3.0 Mpc, and completely unresolved at D=5 Mpc. As an independent test of the sensitivity of our images to low surface brightness galaxies we report on the detection of several dwarf spheroidal galaxies probably located in the Virgo cluster with MV<=-8.0 and muV,h<=26.8 mag/arcsec2. The nature of the previously discovered SECCO 1 stellar system, also likely located in the Virgo cluster, is re-discussed in comparison with these dwarfs. While specific for the SECCO survey, our study may also provide general guidelines for detection of faint stellar systems with 8m class telescopes.Comment: accepted for publication on A&

HII regions within a compact high velocity cloud. A nearly star-less dwarf galaxy?

Within the SECCO survey we identified a candidate stellar counterpart to the Ultra Compact High Velocity Cloud (UCHVC) HVC274.68+74.70-123, that was suggested by Adams et al. (2013) as a possible mini-halo within the Local Group of galaxies. The spectroscopic follow-up of the brightest sources within the candidate reveals the presence of two HII regions whose radial velocity is compatible with physical association with the UVHVC. The available data does not allow us to give a definite answer on the nature of the newly identified system. A few alternative hypotheses are discussed. However, the most likely possibility is that we have found a new faint dwarf galaxy residing in the Virgo cluster of galaxies, which we name SECCO-1. Independently of its actual distance, SECCO-1 displays a ratio of neutral hydrogen mass to V luminosity of M_{HI}/L_V>= 20, by far the largest among local dwarfs. Hence, it appears as a nearly star-less galaxy and it may be an example of the missing links between normal dwarfs and the dark mini halos that are predicted to exist in large numbers according to the currently accepted cosmological model.Comment: Accepted for publication in ApJ Letters. Pdflatex, emulateapj.cls. 6 pages, 3 figures, 2 table

Stemness Features in Liver Cancer

Heterogeneity is a cardinal hallmark of cancer, including primary liver cancer (PLC), and occurs at different layers including putative cell-of-origin. Current evidence suggests that within cellular subpopulations in PLC there are stem-like cells, the cancer stem cells (CSCs). The CSC concept has been recently proposed as an explanation of such intra-tumor heterogeneity. According to this model, CSCs are responsible for tumor initiation, recurrence, metastasis as well as drug-resistance. However, although the CSC hypothesis is intriguing and supported by a large number of experimental studies, there are still open questions regarding the origin of putative CSCs. Since chemo-resistance and recurrence represent major issues in PLC treatment, the development of new therapeutic strategies is needed, for which a good understanding of tumor behavior and in particular of CSCs biology is an imperative prerequisite. In this review we summarize the regulatory pathways that support CSC features in PLC. Moreover, we highlight the key features of hepatic CSC, in terms of enhanced drug-resistance, increased metastatic potential and metabolic rearrangement. Knowledge of the molecular mechanisms underlying CSC biology may provide novel options for PLC combination therapies

The StEllar Counterparts of COmpact high velocity clouds (SECCO) survey. I. Photos of ghosts

We present an imaging survey aimed at searching for the stellar counterparts of recently discovered ultra-compact high-velocity HI clouds (UCHVC). Adams et al. (2013) proposed these clouds to be candidate mini-haloes in the Local Group and/or its surroundings, within a distance range of 0.25-2.0 Mpc. Using the Large Binocular Telescope we obtain wide-field (~ 23' X 23') g- and r-band images of the twenty-five most promising and most compact clouds among the fifty-nine identified by Adams et al. Careful visual inspection of all the images does not reveal any stellar counterpart even slightly resembling LeoP, the only local dwarf galaxy that was found as a counterpart to a previously detected high velocity cloud. Only a possible distant (D>3.0 Mpc) counterpart to HVC274.68+74.70-123 has been identified on our images. The point source photometry in the central 17.3' X 7.7' chips reaches r<= 26.5, and is expected to contain most of the stellar counterparts to the UCHVCs. However, no obvious stellar over-density is detected in any of our fields, in marked contrast to our comparison LeoP field in which the dwarf galaxy is detected at a >30 sigma significance level. Only HVC352.45+59.06+263 may be associated with a weak over-density, whose nature cannot be ascertained with our data. Sensitivity tests shows that our survey would have detected any dwarf galaxy dominated by an old stellar population, with an integrated absolute magnitude M_V<= -8.0, a half-light radius r_h<= 300 pc, and lying within 1.5 Mpc from us, thereby confirming that it is unlikely that the observed UCHVCs are associated with stellar counterparts typical of known Local Group dwarf galaxies.Comment: Accepted for publication by A&A. PdfLaTex. 24 pages, 27 figures. Minor changes; A&A language editin

An optical and HI study of the dwarf Local Group galaxy VV124=UGC4879. A gas-poor dwarf with a stellar disk?

We present a detailed study of the dwarf galaxy VV124, recently recognized as a isolated member of the Local Group. We have obtained deep (r=26.5) wide-field g,r photometry of individual stars with the LBT under sub-arcsec seeing conditions. The Color-Magnitude Diagram suggests that the stellar content of the galaxy is dominated by an old, metal-poor population, with a significant metallicity spread. A very clean detection of the RGB tip allows us to derive an accurate distance of D=1.3 +/- 0.1 Mpc. Combining surface photometry with star counts, we are able to trace the surface brightness profile of VV124 out to ~ 5' = 1.9 kpc radius (where mu_r=30 mag/arcsec^2), showing that it is much more extended than previously believed. Moreover, the surface density map reveals the presence of two symmetric flattened wings emanating from the central elongated spheroid and aligned with its major axis, resembling a stellar disk seen nearly edge-on. We also present HI observations obtained with the WSRT, the first ever of this object. A total amount of 10^6 M_sun of HI gas is detected in VV124. Compared to the total luminosity, this gives a value of M_HI/L_V=0.11, which is particularly low for isolated Local Group dwarfs. The spatial distribution of the gas does not correlate with the observed stellar wings. The systemic velocity of the HI in the region superposed to the stellar main body of the galaxy is V_h=-25 km/s. The velocity field shows substructures typical of galaxies of this size but no sign of rotation. The HI spectra indicates the presence of a two-phase interstellar medium, again typical of many dwarf galaxies.Comment: Accepted for publication in A&A. 19 pages, 20 reduced-resolution figures, pdflatex, A&A style. The full resolution pdf file can be be downloaded from http://www.bo.astro.it/SGR

The Kinematics and Chemistry of Red Horizontal Branch Stars in the Sagittarius Streams

We have selelcted 556 Red Horizontal Branch (RHB) stars along the streams of the Sagittarius dwarf galaxy (Sgr) from SDSS DR7 spectroscopic data using a theoretical model. The metallicity and \alpha-elements distributions are investigated for stars in the Sgr streams and for Galactic stars at the same locations. We find that the Sgr stars have two peaks in the metallicity distribution while the Galactic stars have a more prominent metal-poor peak. Meanwhile, [\alpha/Fe] ratios of the Sgr stars are lower than those of the Galactic stars. Among the Sgr stars, we find a difference in the metallicity distribution between the leading and trailing arms of the Sgr tidal tails. The metallicity and [\alpha/Fe] distribution of the leading arm is similar to that of the Galaxy. The trailing arm is composed mainly of a metal rich component and [\alpha/Fe] is obviously lower than that of the Galactic stars. The metallicity gradient is -(1.8 \pm 0.3)\times10^{-3} dex degree^{-1} in the first wrap of the trailing arm and -(1.5 \pm 0.4)\times10^{-3} dex degree^{-1} in the first wrap of the leading arm. No significant gradient exists along the second wraps of the leading or trailing arms. It seems that the Sgr dwarf galaxy initially lost the metal poor component in the second wrap (older) arms due to the tidal force of our Galaxy and then the metal rich component is disrupted in the first wrap (younger) arms. Finally, we found that the velocity dispersion of the trailing arm from 88^\circ<\Lambda_\odot<112^\circ is \sigma = 9.808 \pm 1.0 km s^{-1}, which is consistent with previous work in the literature.Comment: 24 pages, 10 figures, accepted for publication in Ap

Star Formation in the Starburst Cluster in NGC 3603

We have used new, deep, visible and near infrared observations of the compact starburst cluster in the giant HII region NGC 3603 and its surroundings with the WFC3 on HST and HAWK-I on the VLT to study in detail the physical properties of its intermediate mass (~ 1 - 3 M_sun) stellar population. We show that after correction for differential extinction and actively accreting stars, and the study of field star contamination, strong evidence remains for a continuous spread in the ages of pre-main sequence stars in the range ~ 2 to ~ 30 Myr within the temporal resolution available. Existing differences among presently available theoretical models account for the largest possible variation in shape of the measured age histograms within these limits. We also find that this isochronal age spread in the near infrared and visible Colour-Magnitude Diagrams cannot be reproduced by any other presently known source of astrophysical or instrumental scatter that could mimic the luminosity spread seen in our observations except, possibly, episodic accretion. The measured age spread and the stellar spatial distribution in the cluster are consistent with the hypothesis that star formation started at least 20-30 Myrs ago progressing slowly but continuously up to at least a few million years ago. All the stars in the considered mass range are distributed in a flattened oblate spheroidal pattern with the major axis oriented in an approximate South-East - North-West direction, and with the length of the equatorial axis decreasing with increasing age. This asymmetry is most likely due to the fact that star formation occurred along a filament of gas and dust in the natal molecular cloud oriented locally in this direction.Comment: 21 pages, 19 figures, accepted for publication in Astrophysics & Space Scienc

Erratum: A very dark stellar system lost in Virgo: kinematics and metallicity of SECCO 1 with MUSE

Large scale structure and cosmolog

Dysregulation of Iron Metabolism in Cholangiocarcinoma Stem-like Cells

Cholangiocarcinoma (CCA) is a devastating liver tumour arising from malignant transformation of bile duct epithelial cells. Cancer stem cells (CSC) are a subset of tumour cells endowed with stem-like properties, which play a role in tumour initiation, recurrence and metastasis. In appropriate conditions, CSC form 3D spheres (SPH), which retain stem-like tumour-initiating features. Here, we found different expression of iron proteins indicating increased iron content, oxidative stress and higher expression of CSC markers in CCA-SPH compared to tumour cells growing as monolayers. Exposure to the iron chelator desferrioxamine decreased SPH forming efficiency and the expression of CSC markers and stem-like genes, whereas iron had an opposite effect. Microarray profiles in CCA samples (n = 104) showed decreased H ferritin, hepcidin and ferroportin expression in tumours respect to surrounding liver, whereas transferrin receptor was up-regulated. Moreover, we found a trend toward poorer outcome in CCA patients with elevated expression of ferritin and hepcidin, two major proteins of iron metabolism. These findings, which represent the first evidence of a role for iron in the stem cell compartment as a novel metabolic factor involved in CCA growth, may have implications for a better therapeutic approach

Genetic Manipulation of Schistosoma haematobium, the Neglected Schistosome

More people are infected with Schistosoma haematobium than other major human schistosomes yet it has been less studied because of difficulty in maintaining the life cycle in the laboratory. S. haematobium might be considered the ‘neglected schistosome’ since minimal information on the genome and proteome of S. haematobium is available, in marked contrast to the other major schistosomes. In this report we describe tools and protocols to investigate the genome and genetics of this neglected schistosome. We cultured developmental stages of S. haematobium, and investigated the utility of introducing gene probes into the parasites to silence two model genes. One of these, firefly luciferase, was a reporter gene whereas the second was a schistosome gene encoding a surface protein, termed Sh-tsp-2. We observed that both genes could be silenced – a phenomenon known as experimental RNA interference (RNAi). These findings indicated that the genome of S. haematobium will be amenable to genetic manipulation investigations designed to determine the function and importance of genes of this schistosome and to investigate for novel anti-parasite treatments