Bacteriocinas producidas por Pseudonomas aeruginosa y su acción inhibitoria frente a Helicobacter pylori

Las bacteriocinas definidas como proteínas con un modo de acción bactericida, fueron descritas por primera vez en Escherichia coli y posteriormente en bacterias gram positivas. Estas poseen un espectro de inhibición relativamente reducido, actuando sobre cepas distintas de la misma especie productora, sin embargo algunas son capaces de actuar frente a especies de otros géneros, como lo observado en bacterias lácticas, cuyas bacteriocinas son ampliamente usadas en la conservación de alimentos. Esta característica particular de las bacteriocinas de actuar frente a otros microorganismos fuera de su especie también es compartida por algunas bacterias gram negativas como Pseudomonas aeruginosa. Las piocinas, bacteriocinas producidas por Pseudomonas aeruginosa, que presentan gran similitud por su analogía estructural con las colas de los bacteriófagos y bacteriocinas de E. coli (colicinas), poseen la capacidad de inhibir a otros microorganismos como Neisseria gonorrhoeae, Neisseria meningitidis, Haemophilus ducreyi, y Haemophilus influenzae. Este trabajo estudio el posible rol de las piocinas en el crecimiento de Helicobacter pylori, empleando extractos crudos de 32 cepas de Pseudomonas aeruginosa obtenidos de 3 hábitat distintos (agua, suelo y clínico). Para lograr el objetivo se realizaron pruebas de caracterización de los extractos crudos y determinación molecular por PCR, Se identificaron 9 cepas de Pseudomonas aeruginosa con efecto inhibitorio de origen ambiental; las pruebas realizadas de termo resistencia a diferentes temperaturas, tratamiento enzimático (tripsina), junto al análisis molecular de genes de piocinas, demuestran que las piocinas no están comprometidas con este efecto. El compuesto inhibitorio presente en el extracto crudo de Pseudomonas aeruginosa cepa 12S posee actividad bactericida frente a Helicobacter pylori ATCC 43504 y es de naturaleza no proteica.Tesi

SARS-CoV-2 accessory proteins involvement in inflammatory and profibrotic processes through IL11 signaling

15 p.-7 fig.SARS-CoV-2, the cause of the COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. In this study, transcriptomics analysis revealed that both WNT5A and IL11 were significantly up-regulated in A549 cells expressing individual accessory proteins ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). IL11 is a member of the IL6 family of cytokines. IL11 signaling-related genes were also differentially expressed. Bioinformatics analysis disclosed that both WNT5A and IL11 were involved in pulmonary fibrosis idiopathic disease and functional assays confirmed their association with profibrotic cell responses. Subsequently, data comparison with lung cell lines infected with SARS-CoV-2 or lung biopsies from patients with COVID-19, evidenced altered profibrotic gene expression that matched those obtained in this study. Our results show ORF6, ORF8, ORF9b and ORF9c involvement in inflammatory and profibrotic responses. Thus, these accessory proteins could be targeted by new therapies against COVID-19 disease.This research work was funded by the European Commission – NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI+ Salud Global) (COVID-19-117 and SGL2103015), Junta de Andalucía (CV20-20089) and Spanish Ministry of Science project (PID2021-123399OB-I00).Peer reviewe

SARS-CoV-2 accessory proteins involvement in inflammatory and profibrotic processes through IL11 signaling

SARS-CoV-2, the cause of the COVID19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. Transcriptomic analysis revealed that both WNT5A and IL11 were significantly up-regulated in A549 cells expressing individual accessory proteins ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). IL11 signaling-related genes were also differentially expressed. Bioinformatics analysis disclosed that both WNT5A and IL11 were involved in pulmonary fibrosis idiopathic disease. Functional assays confirmed their association with profibrotic cell responses. Subsequently, data comparison with lung cell lines infected with SARS-CoV-2 or lung biopsies from patients with COVID19 evidenced altered gene expression that matched those obtained in this study. Our results show ORF6, ORF8, ORF9b and ORF9c involvement in inflammatory and profibrotic responses. Thus, these accessory proteins could be targeted by new therapies against COVID19 disease.N

SARS-CoV-2 accessory proteins involvement in inflammatory and profibrotic processes through IL11 signaling

SARS-CoV-2, the cause of the COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. In this study, transcriptomics analysis revealed that both WNT5A and IL11 were significantly up-regulated in A549 cells expressing individual accessory proteins ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). IL11 is a member of the IL6 family of cytokines. IL11 signaling-related genes were also differentially expressed. Bioinformatics analysis disclosed that both WNT5A and IL11 were involved in pulmonary fibrosis idiopathic disease and functional assays confirmed their association with profibrotic cell responses. Subsequently, data comparison with lung cell lines infected with SARS-CoV-2 or lung biopsies from patients with COVID-19, evidenced altered profibrotic gene expression that matched those obtained in this study. Our results show ORF6, ORF8, ORF9b and ORF9c involvement in inflammatory and profibrotic responses. Thus, these accessory proteins could be targeted by new therapies against COVID-19 disease

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Red Contapp

El presente investigación está elaborado mediante conocimientos de investigación que obtuvimos en materia de herramienta de búsqueda de la información y el conocimiento adquirido en cada momento vivido en nuestra casa de estudio, ayudándonos a que tengamos un buen nivel de análisis así como también de investigación. La investigación realizada se refiere a nuestro modelo de negocio Llamado RED CONTAPP el cual se puede definir como una aplicación móvil que unirá a personas en búsqueda de servicios contables con nuestros socios contadores que estarán disponibles para poder brindar sus servicios, la característica principal de esta idea de negocio es que podremos agilizar los procesos de búsqueda de contadores y además brindar la confianza de nuestros usuarios que serán atendidos por personas profesionales expertos en la materia. Nuestro interés académico se basa en las problemáticas que existen en el campo, donde las personas no mantienen un adecuado control de sus ingresos y muchas veces la Sunat realiza multas que perjudican el trabajo de las personas, es por ello que REd Contapp no solamente desea ganancias económicas sino sociales, ya que con la ayuda de un contador las personas podrán tener un crecimiento continuo y nuestro país podrá tener mayores recaudaciones para inversiones en obras.The present proyect is elaborated through of knowledge of investigation that we obtained in matter of tool of search of the information and the knowledge acquired in each moment lived in our University, it helping us to that we have a good level of analysis as well as of investigation. The research carried out refers to our business model called RED CONTAPP, which can be defined as a mobile application that will connected people in search of accounting services with our accounting partners that will be available to provide their services, the main feature of this business idea is that we can streamline the processes of searching for accountants and also provide the confidence of our users who will be attended by professional experts in the matter. Our academic interest is based on the problems that exist in business, where people do not maintain adequate control of their income and often the Sunat makes fines that harm the work of people, that is why REd Contapp not only wants profits economic but also social, because with the help of an accountant can have a continuous growth and our country may have higher taxes collections for investment in public works. With all of this, Red Contapp will be the application that will revolutionize the way to acquire accounting services.Trabajo de investigació

Heavy flavour decay muon production at forward rapidity in proton–proton collisions at √s=7 TeV

The production of muons from heavy flavour decays is measured at forward rapidity in proton–proton collisions at √s=7 TeV collected with the ALICE experiment at the LHC. The analysis is carried out on a data sample corresponding to an integrated luminosity Lint=16.5 nb−1. The transverse momentum and rapidity differential production cross sections of muons from heavy flavour decays are measured in the rapidity range 2.5<y<4, over the transverse momentum range 2<pt<12 GeV/c. The results are compared to predictions based on perturbative QCD calculations

Multi-strange baryon production in pp collisions at √s=7 TeV with ALICE

A measurement of the multi-strange Ξ− and Ω− baryons and their antiparticles by the ALICE experiment at the CERN Large Hadron Collider (LHC) is presented for inelastic proton–proton collisions at a centre-of-mass energy of 7 TeV. The transverse momentum (pT) distributions were studied at mid-rapidity (|y|6.0 GeV/c. We also illustrate the difference between the experimental data and model by comparing the corresponding ratios of (Ω−+Ω¯+)/(Ξ−+Ξ¯+) as a function of transverse mass

Inclusive J/ψ production in pp collisions at √s=2.76 TeV

The ALICE Collaboration has measured inclusive J/ψ production in pp collisions at a center-of-mass energy √s=2.76 TeV at the LHC. The results presented in this Letter refer to the rapidity ranges |y|<0.9 and 2.5<y<4 and have been obtained by measuring the electron and muon pair decay channels, respectively. The integrated luminosities for the two channels are Linte=1.1 nb−1 and Lintμ=19.9 nb−1, and the corresponding signal statistics are NJ/ψe+e−=59±14 and NJ/ψμ+μ−=1364±53. We present dσJ/ψ/dy for the two rapidity regions under study and, for the forward-y range, d2σJ/ψ/dydpt in the transverse momentum domain 0<pt<8 GeV/c. The results are compared with previously published results at s=7 TeV and with theoretical calculations

Neutral pion and η meson production in proton–proton collisions at √s=0.9 TeV and s=√7 TeV

he first measurements of the invariant differential cross sections of inclusive π0 and η meson production at mid-rapidity in proton–proton collisions at s=0.9 TeV and s=7 TeV are reported. The π0 measurement covers the ranges 0.4<pT<7 GeV/c and 0.3<pT<25 GeV/c for these two energies, respectively. The production of η mesons was measured at s=√7 TeV in the range 0.4<pT<15 GeV/c. Next-to-Leading Order perturbative QCD calculations, which are consistent with the π0 spectrum at s=0.9 TeV, overestimate those of π0 and η mesons at s=√7 TeV, but agree with the measured η/π0 ratio at s=√7 TeV