The Virgin of Altagracia in the Intra Caribbean migration context; An intersectional study of migration and lived religion of Dominico Puerto Rican women

This thesis is an intersectional analysis of gender, religion, migration, and race. It focuses on the experiences of Dominico Puerto Rican women in the context of Intra-Caribbean migration, focusing on their lived religious experiences in this transnational space. I specifically explore their intimate and social reflections on the virgin of Altagracia, as a unifying familiar figure in the new environment that provides comfort for women who have left homeland and reminds women of this devotion in the Dominican Republic. The purpose of this research is to introduce a Global South, Intra Caribbean migratory perspective to current sociological debates on religion and migration (Vásquez 2016, Pasura 2016, Levitt 2009). In this thesis I argue that although many studies about religion and migration focus either on the fixed or hybrid understandings of religion from the Global South to Global North migratory context, this research contends that women develop individualized, situated, and personalized interpretations of religion and spirituality that interconnect with a broader cultural and social interpretations of Altagracia. The thesis provides a contribution to an intersectional analysis of religion, gender, and spirituality, because religion in migration is a subject that has not been central for Social Sciences researchers in this Intra Caribbean context. It explores the relationship between intimate and collective meanings of Altagracia without focusing exclusively on the sociological meanings attributed to her by Catholicism. The research is informed by a feminist methodology, which attempts to address a gap in knowledge around women’s experiences of Intra Caribbean migration. The purpose of this methodology is to bring to the spotlight insights of women who live in the areas of San Juan and Canovanas, locations that have not been sufficiently researched. Original primary data foreground the reflections of Dominican migrant women in Puerto Rico on gender, race, religion, and migration. As suggested by the N vivo data analysis software, the data show that Altagracian rituals are connected to the sacred location of Higüey in Dominican Republic and to the colonial history of Catholicism common to the Spanish Caribbean. These rituals and devotions of Altagracia are reproduced, recreated, and re-evaluated with a gendered personal and self-improvement purpose in mind within the transnational space. This thesis contributes to theories of lived religion as proposed by scholars like (Pasura 2014, Jones 2019, McGuire 2016, Hirschman 2004). Its original contribution to knowledge lies also in the enriched understanding of migrant women’s lives in the Caribbean and it proposes a new awareness of Intra Caribbean migration and its interconnections with gendered religious transnationalism. The data show that women prioritize everyday interpretations of religion and spirituality before organized religion

DNA Methylation Patterns in Cord Blood of Neonates Across Gestational Age Association With Cell-Type Proportions

Background: A statistical methodology is available to estimate the proportion of cell types (cellular heterogeneity) in adult whole blood specimens used in epigenome-wide association studies (EWAS). However, there is no methodology to estimate the proportion of cell types in umbilical cord blood (also a heterogeneous tissue) used in EWAS. Objectives: The objectives of this study were to determine whether differences in DNA methylation (DNAm) patterns in umbilical cord blood are the result of blood cell type proportion changes that typically occur across gestational age and to demonstrate the effect of cell type proportion confounding by comparing preterm infants exposed and not exposed to antenatal steroids. Methods: We obtained DNAm profiles of cord blood using the Illumina HumanMethylation27k BeadChip array for 385 neonates from the Boston Birth Cohort. We estimated cell type proportions for six cell types using the deconvolution method developed by Houseman et al. (2012). Results: The cell type proportion estimates segregated into two groups that were significantly different by gestational age, indicating that gestational age was associated with cell type proportion. Among infants exposed to antenatal steroids, the number of differentially methylated CpGs dropped from 127 to 1 after controlling for cell type proportion. Discussion: EWAS utilizing cord blood are confounded by cell type proportion. Careful study design including correction for cell type proportion and interpretation of results of EWAS using cord blood are critical

Resilience to AD pathology in Top Cognitive Performers

Successful cognitive aging is often thought to result from resistance to the accumulation of pathology, resilience to the effects of pathological accumulation, or some combination of the two. While evidence for resilience has been found in typical aging populations, the oldest-old provide us with a unique window into the role of pathological accumulation in impacting cognition. Here, we aimed to assess group differences in measures of amyloid and tau across older age groups using data from the Alzheimer's Disease Neuroimaging Initiative (ADNI age: 60-89) and The 90+ Study (age: 90-101). Additionally, using the ADNI dataset, we performed exploratory analyses of regional cingulate AV-45 SUVRs to assess if amyloid load in particular areas was associated with Top Cognitive Performance (TCP). Consistent with the literature, results showed no group differences in amyloid SUVRs both regionally and in the whole cortex. For tau with AV-1451, we also observed no differences in Braak composite SUVRs. Interestingly, these relationships persisted in the oldest-old. This indicates that Top Cognitive Performance throughout aging does not reflect resistance to amyloid and tau burden, but that other mechanisms may be associated with protection against amyloid and tau related neurodegeneration

Cognitive Change in a Diverse Group of Individuals Aged 90+: The LifeAfter90 Study

Abstract: Background: The oldest‐old are the fastest growing segment of the elderly population but very little is known about cognition in this age group; particularly in diverse populations. Our goal was to evaluate if domain specific cognitive change was different across different ethnoracial groups in those aged 90+. Method: LifeAfter90 (LA90) is an ongoing cohort of participants aged 90+ who are long‐term members of Kaiser Permanente Northern California participants. Participants (n = 984) were interviewed every 6 months for up to 3.5 years (1‐7 visits). Executive Function (EF) and Verbal Episodic Memory (VEM) were measured every six months using z‐standardized Spanish English Neuropsychological Assessment Scale. Racial/ethnic identify (Asian, Black, Latino, or White participants) was used in linear mixed models with random slopes and intercepts adjusting for baseline age, gender, education, interview mode, and practice effects. Result: Participants were 20% Latino, 23% Black, 24% Asian, 27% White, and 7% other individuals with a mean age of 92.4 (SD = 2.3) and a mean follow up time 1.1 years (Table 1). 39% of the cohort were men, 35% were college educated, and 29% were high school or less educated. Average annual change in EF was ‐0.06 (95% CI: ‐0.12, ‐0.00). Stratified models with Latino as the reference group showed White participants had significantly greater decline in EF (β = ‐0.13; 95%CI:‐0.20,‐0.06), followed by Asian participants (β = ‐0.09; 95%CI:‐0.17,‐0.01). EF scores among Black participants and participants who identified as other declined at a similar rate as Latino participants (β = ‐0.03; 95%CI:‐0.11,‐0.05; β = 0.00; 95%CI:‐0.10, 0.11; respectively) (Table 2: model 2). VEM had an annual change of ‐0.26 (95%CI: ‐0.40, ‐0.13), but there were no significant differences across ethnoracial groups in rate of decline. Conclusion: In this population of individuals aged 90+, decline in EF over the study period varied across ethnoracial group with White participants experiencing the fastest decline and Black, Latino, and other participants experiencing the slowest decline. Continued follow up will identify if there are differences in risk of cognitive impairment in this diverse population of oldest‐old. The results suggest the disparities in cognitive aging for those aged 90+ don’t mirror disparities seen in younger‐elderly ages

Blood Pressure Circadian Variation, Cognition and Brain Imaging in 90+ Year-Olds

Purpose: To analyze the relationship between blood pressure (BP) variables, including circadian pattern, and cognition in 90+ year-olds.Methods: Twenty-four hour ambulatory BP monitoring was completed on 121 participants drawn from a longitudinal study of aging and dementia in the oldest-old. Various measures of BP and its variability, including nocturnal dipping, were calculated. Each person was given both a neuropsychological test battery covering different cognitive domains and a neurological examination to determine cognitive status. Seventy-one participants had a brain magnetic resonance imaging (MRI) scan.Results: Participants ranged in age from 90 to 102 years (mean = 93), about two-thirds were female, and nearly 80% had at least some college education. Mean nocturnal dips differed significantly between cognitively normal (n = 97) and impaired individuals (n = 24), with cognitively normal participants having on average greater nocturnal dips [6.6% vs. 1.3%, p = 0.006 for systolic BP (SBP); 11% vs. 4.4%, p = 0.002 for diastolic BP (DBP)]. Nocturnal dips were also related to performance on select cognitive test scores (especially those related to language, recent memory and visual-spatial ability), with individuals who performed below previously established median norms having significantly smaller nocturnal dips (both SBP and DBP) than those above the median. DBP reverse dippers had larger mean white matter hyperintensities (WMH as percent of total brain volume; 1.7% vs. 1.2%, 1.1% and 1.0% in extreme dippers, dippers, non-dippers) and a greater proportion had lobar cerebral microbleeds (CMBs; 44% vs. 0%, 7%, 16%, p < 0.05). Impaired participants had higher mean WMH than those with normal cognition (1.6% vs. 1.0% p = 0.03) and more tended to have CMB (31% vs. 20%, p = n.s.).Conclusion: These findings suggest that cognitive dysfunction is associated with dysregulation in the normal circadian BP pattern. Further study is warranted of the potential role of WHM and CMB as mediators of this association

Positive Impact of Physical Exercise for Managing Stereotypies in Dogs

Stereotypies in dogs are repetitive behavioral patterns that occur always in the same sequence but do not play any role. This altered behavior may thus take over the animal’s life, changing its sleep patterns and eating habits. The drive to adopt the specific behavior becomes increasingly stronger, to the extent that the animal may sometimes even mutilate parts of its own body, such as the tail and limbs. The overall approach to the problem through retraining of owners, environmental modification and enrichment and proper use of psychotropics proved necessary for restoring the patients’ balance. However, it was also necessary to introduce a physical exercise routine with a canine motorized treadmill and/or daily walks. Although exercise itself may contribute to increased anxiety in the animal, the potential benefits justify considering its introduction for managing dogs with stereotypies.Fil: Ostrovsky, G.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Alvarez, F.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Risso, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Pellegrino, Francisco Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marchionni, M.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Aversa, D.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Corrada, Yanina Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Yanagi: Fast and interpretable segment-based alternative splicing and gene expression analysis

Ultra-fast pseudo-alignment approaches are the tool of choice in transcript-level RNA sequencing (RNA-seq) analyses. Unfortunately, these methods couple the tasks of pseudo-alignment and transcript quantification. This coupling precludes the direct usage of pseudo-alignment to other expression analyses, including alternative splicing or differential gene expression analysis, without including a non-essential transcript quantification step.https://doi.org/10.1186/s12859-019-2947-