Human cytomegalovirus elicits fetal γδ T cell responses in utero

The fetus and infant are highly susceptible to viral infections. Several viruses, including human cytomegalovirus (CMV), cause more severe disease in early life compared with later life. It is generally accepted that this is a result of the immaturity of the immune system. γδ T cells are unconventional T cells that can react rapidly upon activation and show major histocompatibility complex–unrestricted activity. We show that upon CMV infection in utero, fetal γδ T cells expand and become differentiated. The expansion was restricted to Vγ9-negative γδ T cells, irrespective of their Vδ chain expression. Differentiated γδ T cells expressed high levels of IFN-γ, transcription factors T-bet and eomes, natural killer receptors, and cytotoxic mediators. CMV infection induced a striking enrichment of a public Vγ8Vδ1-TCR, containing the germline-encoded complementary-determining-region-3 (CDR3) δ1–CALGELGDDKLIF/CDR3γ8–CATWDTTGWFKIF. Public Vγ8Vδ1-TCR–expressing cell clones produced IFN-γ upon coincubation with CMV-infected target cells in a TCR/CD3-dependent manner and showed antiviral activity. Differentiated γδ T cells and public Vγ8Vδ1-TCR were detected as early as after 21 wk of gestation. Our results indicate that functional fetal γδ T cell responses can be generated during development in utero and suggest that this T cell subset could participate in antiviral defense in early life

Role of the virology laboratory in the diagnosis of central nervous system infections

SCOPUS: re.jinfo:eu-repo/semantics/publishe

SYNDROME D'IMMUNODEFICIENCE ACQUISE EN MILIEU HOSPITALIER: ASPECTS VIROLOGIQUES

SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

Screening of semen donors for infectious diseases

SCOPUS: cp.jinfo:eu-repo/semantics/publishe

Hepatitis B vaccination in persons with isolated hepatitis B core antibodies [1] (multiple letters)

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Screening standards in assisted reproductive technologies: Is the British Andrology Society recommendation to recruit cytomegalovirus negative semen donors only, a reasonable one?

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Is matching between women and donors feasible to avoid cytomegalovirus infection in artificial insemination with donor semen?

SCOPUS: cp.jinfo:eu-repo/semantics/publishe

Transmission materno-foetale et périnatale des maladies sexuellement transmises. Infection par cytomégalovirus.

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Place des anticorps anti-HC dans le diagnostic différentiel des hépatites aigues et chroniques

SCOPUS: cp.jinfo:eu-repo/semantics/publishe

Comparaison de deux techniques radioimmunologiques pour le diagnostic rapide de l'hépatite A: Révision du diagnostic étiologique dans 83 cas d'hépatite aigue

Two radioimmunoassays for the detection of anti-hepatitis A IgM were evaluated. In one of them, IgM were detected by absorption of IgG on Staphylococcus aureus protein A. The other one is a commercial kit (HAVAB-M, Abbott) where the IgM are first absorbed on polystyrene beads coated with goat anti-human antibody specific for human IgM. On 83 incidents of acute hepatitis, the second technique was proved to be more sensitive: 3 more acute hepatitis A were diagnosed, and the possibility of detection of IgM in 2 late sera and 1 early serum.Deux techniques radioimmunologiques de détection des IgM sériques anti-hépatite A ont été évaluées: l'une comportant l'absorption des IgG par la protéine A de Staphylococcus aureus, l'autre (HAVAB-M Abbott) l'absorption des IgM par un immunsérum spécifique de chèvre.Sur 83 cas d'hépatite aiguë, la seconde technique s'est révélée plus sensible.SCOPUS: NotDefined.jSCOPUS: ar.jinfo:eu-repo/semantics/publishe