Pedagogical Political Project: a look for inclusive education in the countryside

The Pedagogical Political Project (PPP) is characterized by being the guiding document of pedagogical practice at school, carrying with it an intention and contributing to the creation of school identity. This study aims to identify the perspectives of inclusive education and rural education present in the PPPs of schools located in the countryside of the municipality of Uruguaiana, Rio Grande do Sul, Brazil. This is a study with a qualitative approach, characterized in terms of objectives as exploratory and descriptive and in terms of procedures as a documentary research. The research was developed in 10 schools located in the countryside, being 6 municipality and 4 state. As for the results, advances are observed, such as methodological and evaluative adaptations with a view to promoting inclusive education. However, the perspectives of rural education and rural schools still need more attention, as they are present in a minority of PPPs, evidencing the little visibility given to the theme. Finally, it is concluded that the PPP as a guiding document has the role of establishing and contemplating the specificities of the school community, enhancing the relationship between the school environment and the appreciation of the countryside, without imposing a standardized school educational method

Home Exercise Prescription for Children with Down Syndrome and Physical and Occupational Therapists\u27 Perspective on Caregiver Compliance

Background: Children with Down Syndrome (DS) may receive physical and occupational therapy intervention to increase motor skill development and functional independence. Therapeutic effectiveness and outcomes are positively correlated with levels of compliance to therapist-prescribed home programs. Within the DS community, caregiver involvement (usually a parent or guardian) is a primary variable affecting home program compliance. A dearth of research on this topic identified a need to investigate home program compliance among caregivers to facilitate maximal treatment efficacy. This study specifically explores pediatric physical and occupational therapists’ approach to home program prescription and their impressions on caregiver compliance. Methods/Approaches: Research for this project is ongoing. Data points are being gathered from a Qualtrics survey administered to pediatric physical and occupational therapists. Results: Preliminary findings suggest that strong barriers to compliance include home activity programs that are deemed too extensive, which is consistent with prior research by Lorden et al. (2020) that demonstrated a positive association between caregiver buy-in and compliance. Conclusion: Our study posits that convenience of home program activities as well as access to and comprehension of performance instructions will be positively associated with therapist perceptions of increased caregiver compliance

Characterization of the Staphylococcus aureus Heat Shock, Cold Shock, Stringent, and SOS Responses and Their Effects on Log-Phase mRNA Turnover

Despite its being a leading cause of nosocomal and community-acquired infections, surprisingly little is known about Staphylococcus aureus stress responses. In the current study, Affymetrix S. aureus GeneChips were used to define transcriptome changes in response to cold shock, heat shock, stringent, and SOS response-inducing conditions. Additionally, the RNA turnover properties of each response were measured. Each stress response induced distinct biological processes, subsets of virulence factors, and antibiotic determinants. The results were validated by real-time PCR and stress-mediated changes in antimicrobial agent susceptibility. Collectively, many S. aureus stress-responsive functions are conserved across bacteria, whereas others are unique to the organism. Sets of small stable RNA molecules with no open reading frames were also components of each response. Induction of the stringent, cold shock, and heat shock responses dramatically stabilized most mRNA species. Correlations between mRNA turnover properties and transcript titers suggest that S. aureus stress response-dependent alterations in transcript abundances can, in part, be attributed to alterations in RNA stability. This phenomenon was not observed within SOS-responsive cells

Characterizing the Effect of the Staphylococcus aureus Virulence Factor Regulator, SarA, on Log-Phase mRNA Half-Lives

Bacterial pathogens regulate virulence factor expression at both the level of transcription initiation and mRNA processing/turnover. Within Staphylococcus aureus, virulence factor transcript synthesis is regulated by a number of two-component regulatory systems, the DNA binding protein SarA, and the SarA family of homologues. However, little is known about the factors that modulate mRNA stability or influence transcript degradation within the organism. As our entree to characterizing these processes, S. aureus GeneChips were used to simultaneously determine the mRNA half-lives of all transcripts produced during log-phase growth. It was found that the majority of log-phase transcripts (90%) have a short half-life (<5 min), whereas others are more stable, suggesting that cis- and/or trans-acting factors influence S. aureus mRNA stability. In support of this, it was found that two virulence factor transcripts, cna and spa, were stabilized in a sarA-dependent manner. These results were validated by complementation and real-time PCR and suggest that SarA may regulate target gene expression in a previously unrecognized manner by posttranscriptionally modulating mRNA turnover. Additionally, it was found that S. aureus produces a set of stable RNA molecules with no predicted open reading frame. Based on the importance of the S. aureus agr RNA molecule, RNAIII, and small stable RNA molecules within other pathogens, it is possible that these RNA molecules influence biological processes within the organism