Studies in the growth of grapes

During the grape season of 1922 in South Africa the question of the ripeness of grapes arose in connection with their export. In this country the export regulations with regard to grapes stipulate that "no unripe grapes will be passed by the Inspector ",while the general regulations concerning fruits for export provide that "all fruit shall be in a sound condition, fully developed and not too unripe." In America a legal standard has been set up whereby the sugar content of the juice must be at least 16 - 17° Balling according to the variety of the grape( 1°). This law was framed to prevent the indiscriminate marketing of green immature grapes and has apparently proved successful in practice.In view of the indefinite nature of the terms "ripe" and "unripe ", it is clear that any attempt to apply regulations, involving such terms, must be an entirely arbitrary proceeding depending to a large extent upon the personal interpretation of such factors, and consequently will undoubtedly be subject to considerable variations. The problem of what constitutes a ripe grape must therefore be solved before an attempt can be made with any chance of success to apply regulations which involve an estimation of the degree of ripeness of the fruit. Since sweetness is a desirable quality in any fruit, attention is generally focused upon the sugar- content, more especially as the flavour appears to be associated with this factor. At the same time it is important to remember that the intensity of a sensation such as sweetness is considerably '\modified by the presence of an acid or sour substance(36). It rn is, therefore, clear that the condition of ripeness of a fruit must also depend upon the acidity of the juice. It may be concluded that a high sugar-content and a low acidity are desirable for the production of a mild sweet flavour in the fruit.At the present time there is no generally established method for determining when grapes have reached a condition suitable for export. In addition to the taste which may be subject to wide variations, the colour, firmness and size all influence the picker's decision. When the appearance and attractiveness of the product are to be considered the physical aspect of the berry must be given due weight, but the final decision regarding the maturity of the fruit must depend essentially upon the quality and flavour of the juice. In order to obtain an idea of the limits of some of the factors which influence "the condition of ripeness" in connection with table varieties of grapes, an investigation into the changes which occurred during the ripening of grapes was undertake.n. Preliminary work was carried out during the 1923 season and the work was continued during the 1925 and 1926 seasons in order to determine the extent to which these limits are affected by seasonal conditions. In 1927 similar work was carried out for the purpose of studying the effects due to a variation in locality

Antigen detection ELISAs: pretreatment of serum to reduce interference by specific host antibodies

The pretreatment of serum to reduce interference by specific host antibodies was investigated as a means of improving the sensitivity of antigen detection ELISAs whilst screening serum samples. Four antigen detection assays based on monoclonal antibodies directed against antigens of the bovine filariid Onchocerca gibsoni were used in this study and of these, three assays suffered a dramatic drop in sensitivity when detecting male O. gibsoni antigen in the presence of bovine serum as compared with antigen in buffer. A number of methods for pretreating serum to eliminate the problem of antibody interference with antigen detection were attempted, including heat and alkali treatments, detergent treatment of heat treated samples and the use of a reducing agent. The pretreatment of serum by boiling for 5 minutes in the presence of an equal volume of 0.1 M Na2EDTA pH 4.0 and recovery of the supernatant fluid following centrifugation at 16000 g was the most effective method of restoring the sensitivity of each of these three assays whilst screening bovine serum. Pretreatment of serum using this method produced up to a 512-fold increase in sensitivity compared with results obtained in assays with non-treated serum.Edna McConnell Clark Foundatio

The use of monoclonal antibody-based ELISAs to monitor the efficacy of drugs against male Onchocerca gibsoni in vitro

Four monoclonal antibodies directed against antigens of Onchocerca gibsoni were used in antigen detection ELISAs to monitor the efficacy of CGP 20309, CGP 20376, CGP 21833, CGP 24589 and CGP 26702 at 5 μg/ml against male O. gibsoni in vitro. No significant differences (P < 0.05) in antigen output between treated and control groups of parasites were recorded. However, consistently higher levels of antigen from treated (CGP 21833) as compared to control parasites were measured with all four assays, with differences being higher in the first 2 to 3 days post treatment than subsequently. The sensitivity of comparisons between groups was reduced by the high variability in output of antigen both between worms and also from the same worm, in part as a result of mechanical damage to worms sustained during collection or manipulation in vitro. This problem was reduced by zero handling once worms were established in vitro and it is recommended that future work should include a 24 to 48 hour period before treatment commences to detect raised antigen levels associated with physically damaged parasites so they can be excluded. It was concluded that this type of assay has no intrinsic technical or logistical advantage over other published methods of assessing drug-related damage in in vitro filarial screens. Nevertheless, further work using antigen detection ELISAs in this context is justified since these assays, unlike all other methods of assessing drug-induced damage in vitro, have direct application for use in identifying chemotherapeutic effects against similar parasites in vivo.Edna McConnell Clark Foundatio

A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis

A monoclonal antibody, Og4C3, directed against antigens of Onchocerca gibsoni (but not phosphorylcholine) has been used in a sandwich ELISA to detect a circulating antigen of Wuchereria bancrofti in human serum. The interfering effect of host antibody was reduced by first boiling one part of serum for 5 min in the presence of three parts of 0.1 M Na2EDTA, pH 4.0. A total of 119 sera from individuals and 8 pooled sera from clinical and/or parasitologically defined cases of filariasis, plus 8 individual and 1 pooled endemic control sera, all from the filariasis serum bank of the World Health Organisation, as well as 20 non-endemic control sera, were screened with the assay. Circulating antigen was detected in serum from people infected with W. bancrofti but not Brugia malayi, B. timori, O. volvulus or Loa loa, and not in endemic or non-endemic controls. Of the 68 sera from W. bancrofti-infected subjects, 55/55 parasitologically confirmed and 12/13 clinically confirmed but amicrofilaraemic cases reacted in the assay. A weak but significant correlation (r2 = 0.4016) was found between numbers of microfilariae in blood and detectable levels of circulating antigen from patients with bancroftian filariasis.Edna McConnell Clark Foundatio

The use of monoclonal antibody-based ELISAs to monitor chemotherapeutic effects in the bovine-Onchocerca gibsoni drug screen

Three monoclonal antibodies directed towards antigens of Onchocerca gibsoni were used in antigen detection ELISAs to detect parasite antigens in sera from 100 cattle infected with O. gibsoni, in trials with the filaricidal compounds CGP 6140, CGP 20309, CGP 20376, CGP 21833, CGP 24589 and CGP 26702. Measurable levels of parasite antigens were highly variable, both within and between treatment and control groups of animals, with no consistent trends which related to time after treatment, micro or macrofilaricidal effects against O. gibsoni, or dose rate for any of the compounds used. It was concluded that these assays were unsuitable as a method of identifying drug-induced damage to O. gibsoni following the administration of these compounds. A detailed protocol for selecting suitable assays is discussed.Edna McConnell Clark Foundatio

Methods of producing new nutrient data for popularly consumed multi ethnic foods in the UK

Minority ethnic groups in UK disproportionately suffer from nutrition related diseases compared to the mainstream population, contributing to widening health inequalities. However, reliable nutrient composition data of the traditional foods of these ethnic groups, which play an important part in their diets, is lacking. This makes it impossible to provide adequate and culturally acceptable nutrition interventions to reduce prevalent metabolic disorders. This study aimed to identify and analyse popularly consumed African and Caribbean foods in the UK for macro and micronutrients. Various approaches including focus group discussions, individual interviews and 24 hr dietary recalls were used to identify traditional foods. Defined criteria were used to prioritise and prepare 33 composite samples (26 dishes, 4 snacks and 3 beverages) for nutrient analyses in a UK accredited laboratory. This study methodology is novel because it uses various approaches to generate new data of commonly consumed ethnic foods and traditional recipes. In addition, the approach used in preparation of the food samples enhanced their authenticity and representativeness compared to previously published work. This paper describes the procedures undertaken and analytical methods used to develop a multi ethnic nutrient data for inclusion in UK food composition tables

Views on risk, prevention and management of type 2 diabetes among UK Black Caribbeans

Background: Development of effective, culturally-tailored interventions to address excess risk of type 2 diabetes among Black Caribbeans in the United Kingdom (UK) requires understanding of the views and experiences of the target population. We explored the social context of views on risk, prevention and management of type 2 diabetes among this ethnic group. Methods: The Food, Diabetes and Ethnicity (FOODEY) study included 56 Black Caribbean men and women aged 24-90 years (21 (38%) diagnosed with diabetes or pre-diabetes). Nine focus groups were conducted in community hubs in Leeds, Bradford, Birmingham, and Huddersfield, UK. Inductive themes were identified through thematic analysis of transcripts. Results: While family history was considered a key risk factor, there was a clearly articulated view of the interaction between “bad genes” and unfavourable dietary and physical activity (PA) habits. Rich descriptions of food habits and food related negotiations among family and friends included cost and low availability as barriers to maintaining traditional foodways, and high intake of convenience foods. The perception that diabetes risk was greater in the UK than in home countries was widely held, and it was felt that this was due to the lack of PA, cold weather and stress due to racism experienced in the UK. The Caribbean was deemed a suitable setting for consuming traditional foods and high sugar intake as this was mitigated by active living, organic vegetable consumption, and the hot climate. Trust in health professionals’ diabetes advice was evident, however behaviour change was preferred to medication, and the need for choice regarding healthcare decisions was emphasised. Faith beliefs coincided with acceptability of health-related advice and underpinned views on personal responsibility for health. Conclusions: Complex explanatory models of risk, encompassing lifestyle, economic, cultural, religious and psychosocial contexts, have implications for developing interventions to address type 2 diabetes among UK Black Caribbeans

Nutrient Composition of Popularly Consumed African and Caribbean Foods in The UK.

(1) Background: Traditional foods are important in the diets of Black Africans and Caribbeans and, more widely, influence UK food culture. However, little is known about the nutritional status of these ethnic groups and the nutrient composition of their traditional foods. The aim was to identify and analyse African and Caribbean dishes, snacks and beverages popularly consumed in the UK for energy, macronutrients and micronutrients. (2) Methods: Various approaches including focus group discussions and 24-h dietary recalls were used to identify traditional dishes, snacks, and beverages. Defined criteria were used to prioritise and prepare 33 composite samples for nutrient analysis in a UK accredited laboratory. Quality assurance procedures and data verification were undertaken to ensure inclusion in the UK nutrient database. (3) Results: Energy content ranged from 60 kcal in Malta drink to 619 kcal in the shito sauce. Sucrose levels did not exceed the UK recommendation for adults and children. Most of the dishes contained negligible levels of trans fatty acid. The most abundant minerals were Na, K, Ca, Cu, Mn and Se whereas Mg, P, Fe and Zn were present in small amounts. (4) Conclusion: There was wide variation in the energy, macro- and micronutrients composition of the foods analysed

The multikinase inhibitor midostaurin (PKC412A) lacks activity in metastatic melanoma: a phase IIA clinical and biologic study

Midostaurin (PKC412A), N-benzoyl-staurosporine, potently inhibits protein kinase C alpha (PKCα), VEGFR2, KIT, PDGFR and FLT3 tyrosine kinases. In mice, midostaurin slows growth and delays lung metastasis of melanoma cell lines. We aimed to test midostaurin's safety, efficacy and biologic activity in a Phase IIA clinical trial in patients with metastatic melanoma. Seventeen patients with advanced metastatic melanoma received midostaurin 75 mg p.o. t.i.d., unless toxicity or disease progression supervened. Patient safety was assessed weekly, and tumour response was assessed clinically or by CT. Tumour biopsies and plasma samples obtained at entry and after 4 weeks were analysed for midostaurin concentration, PKC activity and multidrug resistance. No tumour responses were seen. Two (12%) patients had stable disease for 50 and 85 days, with minor response in one. The median overall survival was 43 days. Seven (41%) discontinued treatment with potential toxicity, including nausea, vomiting, diarrhoea and/or fatigue. One patient had >50% reduction in PKC activity. Tumour biopsies showed two PKC isoforms relatively insensitive to midostaurin, out of three patients tested. No modulation of multidrug resistance was demonstrated. At this dose schedule, midostaurin did not show clinical or biologic activity against metastatic melanoma. This negative trial reinforces the importance of correlating biologic and clinical responses in early clinical trials of targeted therapies