Nighttime foraging by deep diving echolocating odontocetes off the Hawaiian islands of Kauai and Ni’ihau as determined by passive acoustic monitors

Remote autonomous ecological acoustic recorders (EARs) were deployed in deep waters at five locations around the island of Kauai and one in waters off Ni'ihau in the main Hawaiian island chain. The EARs were moored to the bottom at depths between 400 and 800 m. The data acquisition sampling rate was 80 kHz and acoustic signals were recorded for 30 s every 5 min to conserve battery power and disk space. The acoustic data were analyzed with the M3R (Marine Mammal Monitoring on Navy Ranges) software, an energy-ratio-mapping algorithm developed at Oregon State University and custom MATLAB programs. A variety of deep diving odontocetes, including pilot whales, Risso's dolphins, sperm whales, spinner and pan-tropical spotted dolphins, and beaked whales were detected at all sites. Foraging activity typically began to increase after dusk, peaked in the middle of the night and began to decrease toward dawn. Between 70% and 84% of biosonar clicks were detected at night. At present it is not clear why some of the known deep diving species, such as sperm whales and beaked whales, concentrate their foraging efforts at night. (C) 2013 Acoustical Society of America.Keywords: Cross seamount, Signals, Clicks, Finned pilot whales, Beaked whales, Rissos dolphin, Temporal patterns, Mesoplodon densirostris, Behavior, Soun

Understanding EU legal integration/disintegration : in search of new perspectives

This report summarises the UACES/ James Madison Trust EUFutures Research Network Launch Workshop entitled 'Understanding legal integration/disintegration: in search of new perspectives'. The event consisted of four panels on 'Interdisciplinary research on EU law', 'Research Methods and EU law', 'Understanding the EU's integration processes' and 'Understanding EU law through soft law, discourse, ideas & beliefs', respectively. The future of EU legal integration is at a significant juncture with the departure of the UK, substantial rule of law challenges, internal and external crises, and an increasingly apathetic multilateral legal order. There is increased recognition amongst EU lawyers, who have historically limited themselves to doctrinal analysis and legal hermeneutics, that methodology plays an essential role in order to understand EU integration and shape its future. The question remains though how to connect interdisciplinary approaches to EU law, policy and politics. How should EU law (as an object) be studied? What are the respective merits of each discipline (political science, sociology, economy, history) in explaining the way EU law is created, applied, used, transformed in the process of EU integration? What is the added value of bringing together different approaches to law? In particular, how can EU law (as an academic discipline) open itself up to the methods of the social sciences and what, in return, can law offer to our understanding of EU studies more widely? In order to answer these questions, EUFutures brings together scholars for this workshop to: reflect on the future methodological direction(s) of EU law and EU integration and consider both how law could open itself up to methodologies from other disciplines, and what legal analysis could offer political, economic and historical approaches

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Influences of Spatial and Temporal Variability of Sound Scattering Layers on Deep Diving Odontocete Behavior

Ph.D. University of Hawaii at Manoa 2016.Includes bibliographical references.Patchiness of prey can influence the behavior of a predator, as predicted by the optimal foraging theory which states that an animal will maximize the energy gain while minimizing energy loss. While this relationship has been studied and is relatively well understood in some terrestrial systems, the same is far from true in marine systems. It is as important to investigate this in the marine realm in order to better understand predator distribution and behavior. Micronekton, organisms from 2 – 20 cm, might be a key component in understanding this as it is potentially an essential link in the food web between primary producers and higher trophic levels, including cephalopods which are primary prey items of deep diving odontocetes (toothed whales). My dissertation assesses the spatial and temporal variability of micronekton in the Northwestern Hawaiian Islands (NWHI), the Main Hawaiian Islands’ (MHI) Island of Hawaii, and the Gulf of Mexico (GOM). Additionally it focuses on understanding the relationship between the spatial distribution of micronekton and environmental and geographic factors, and how the spatial and temporal variability of this micronekton relates to deep diving odontocete foraging. I used both an active Simrad EK60 echosounder system to collect water column micronekton backscatter and a passive acoustic system to detect the presence of echolocation clicks from deep diving beaked, sperm, and short-finned pilot whales. My results provide insight into what might be contributing to hotspots of micronekton which formed discrete layers in all locations, a shallow scattering layer (SSL) from the surface to about 200 m and a deep scattering layer (DSL) starting at about 350 m. In both the GOM and the NWHI, the bathymetry and proximity to shore influenced the amount of micronekton backscatter with locations closer to shore and at shallower depths having higher backscatter. We found in all three locations that some species of deep diving odontocetes were searching for prey in these areas with higher micronekton backscatter. Beaked whales in the NWHI, short-finned pilot whales in the NWHI and MHI, and sperm whales in the GOM where present in areas of higher micronekton backscatter. These hotspots of backscatter may be good predictors of the distribution of some deep-diving toothed whale foragers since the hotspots potentially indicate a food web supporting the prey of the cetaceans

The deubiquitinase emperor's thumb is a regulator of apoptosis in Drosophila

We have characterized the gene emperor's thumb (et) and showed that it is required for the regulation of apoptosis in Drosophila. Loss-of-function mutations in et result in apoptosis associated with a decrease in the concentration of DIAP1. Overexpression of one form of et inhibits apoptosis, consistent with et having an anti-apoptotic function; however, overexpression of a second form of et induces apoptosis, indicating that the two forms of et may have competing functions. et encodes a protein deubiquitinase, suggesting it regulates apoptosis by controlling the stability of apoptotic regulatory proteins

Outcomes of Extended Pedicle Technique vs Free Nipple Graft Reduction Mammoplasty for Patients With Gigantomastia.

BACKGROUND: Optimal reduction mammoplasty techniques to treat patients with gigantomastia have been debated and can involve extended pedicles (EP) or free nipple grafts (FNG). OBJECTIVES: The authors compared clinical, patient-reported, and aesthetic outcomes associated with reduction mammoplasty employing EP vs FNG. METHODS: A multi-institutional, retrospective study of adult patients with gigantomastia who underwent reduction mammoplasty at 2 tertiary care centers from 2017 to 2020 was performed. Gigantomastia was defined as reduction weight \u3e1500 g per breast or sternal notch-to-nipple distance ≥40 cm. Surgeons at 1 institution employed the EP technique, whereas those at the other utilized FNG. Baseline characteristics, preoperative and postoperative BREAST-Q, and clinical outcomes were collected. Aesthetic outcomes were assessed in 1:1 propensity score-matched cases across techniques. Preoperative and postoperative photographs were provided to reviewers across the academic plastic surgery continuum (students to faculty) and non-medical individuals to evaluate aesthetic outcomes. RESULTS: Fifty-two patients met the inclusion criteria (21 FNG, 31 EP). FNG patients had a higher incidence of postoperative cellulitis (23% vs 0%, P \u3c 0.05) but no other differences in surgical or medical complications. Baseline BREAST-Q scores did not differ between groups. Postoperative BREAST-Q scores revealed greater satisfaction with the EP technique (P \u3c 0.01). The aesthetic assessment of outcomes in 14 matched pairs of patients found significantly better aesthetic outcomes in all domains with the EP procedure (P \u3c 0.05), independent of institution or surgical experience. CONCLUSIONS: This multi-institutional study suggests that, compared with FNG, the EP technique for reduction mammoplasty provides superior clinical, patient-reported, and aesthetic outcomes for patients with gigantomastia

An Overview of the Capillary Electrophoresis Process at the DOE Joint Genome Institute Production Genomics Facility: The Dual Operation of the AB 3730xl and GE MegaBACE 4500 DNA Sequence Analyzers

At the center of the Department of Energy's (DOE) Joint Genome Institute (JGI) Production Genomics Facility (PGF), lies a highly efficient and automated production line devoted to the generation of high-quality genomic DNA sequence. The JGI utilizes a dual platform of DNA sequence analyzers within its Production Capillaries group: seventy Applied Biosystems 3730xl and thirty-six GE Healthcare MegaBACE 4500 instruments. The Capillaries group is comprised of eleven employees that are responsible for operating and maintaining both platforms; the group is also involved in the daily monitoring of performance stats and the troubleshooting of DNA sample and instrument related issues. The operation of these high-throughput fluorescence-based DNA sequence analyzers will be assessed on the strengths and benefits of each platform, including instrument overviews of operational parameters and mechanical/component specifications. In addition, instrument setups for production operation, operation schedules, loading, and maintenance strategies as well as the various sequencing strategies for each platform will be compared. Throughput numbers and sequencing quality results will be presented

Characterizing a Foraging Hotspot for Short-Finned Pilot Whales and Blainville’s Beaked Whales Located off the West Side of Hawai‘i Island by Using Tagging and Oceanographic Data

<div><p>Satellite tagging data for short-finned pilot whales (<i>Globicephala macrorhynchus</i>) and Blainville’s beaked whales (<i>Mesoplodon densirostris</i>) were used to identify core insular foraging regions off the Kona (west) Coast of Hawai‘i Island. Ship-based active acoustic surveys and oceanographic model output were used in generalized additive models (GAMs) and mixed models to characterize the oceanography of these regions and to examine relationships between whale density and the environment. The regions of highest density for pilot whales and Blainville’s beaked whales were located between the 1000 and 2500 m isobaths and the 250 and 2000 m isobaths, respectively. Both species were associated with slope waters, but given the topography of the area, the horizontal distribution of beaked whales was narrower and located in shallower waters than that of pilot whales. The key oceanographic parameters characterizing the foraging regions were bathymetry, temperature at depth, and a high density of midwater micronekton scattering at 70 kHz in 400–650 m depths that likely represent the island-associated deep mesopelagic boundary community and serve as prey for the prey of the whales. Thus, our results suggest that off the Kona Coast, and potentially around other main Hawaiian Islands, the deep mesopelagic boundary community is key to a food web that supports insular cetacean populations.</p></div