A stochastic mesoscale model of transiently networked fluids.

Macroscale models of physically networked fluids suffer from the inability to connect directly to mesoscale processes due to nonlinearity and thus the need for closure approximations. In this paper physically networked complex fluids are modeled stochastically at the mesoscale, thus avoiding the need for closure assumptions. In the modeling and simulations the network consists of linear chains of Hookean bead-spring dumbbells. The linear chains break and reform stochastically subject to prescribed local attractive potentials. In the simulations the topology of the network is tracked allowing for quantification of the distribution of chain lengths and of individual dumbbell stretch. This formulation allows for breakage and recombination associated with local stress. Model predictions of the distributions of chain lengths and of dumbbell stretch both in equilibrium and in steady shearing are presented. As well, shear thickening and shear thinning regimes in steady shearing and relaxation after step strain are examined

The Changing Face of Milk Production, Milk Quality and Milking Technology in Brazil

This introductory paper gives an overview of milk production in Brazil and discusses a series of recent regulations implemented to improve milk quality with the purpose of asserting the Brazilian dairy industry as a competitor on the international market. It also points out the economic advantage of setting design guidelines for milking machines that would be best suited to Brazilian crossbred cows.Brazilian Milk Quality, Brazil Dairy Industry, Brazilian Milk Production, Brazilian Milk Prices, Agribusiness, Farm Management, Food Consumption/Nutrition/Food Safety, Industrial Organization, International Development, Political Economy,

Slippage and Migration in Taylor-Couette Flow of a Model for Dilute Wormlike Micellar Solutions

Submitted to J. Non-Newt Fluid Mechanics, June 2005In this paper we explore a model, most appropriate for dilute or semi-dilute worm-like micellar solutions, in an axisymmetric circular Taylor-Couette geometry. This study is a natural continuation of earlier work on rectilinear shear flows. The model, based on a bead-spring microstructure with nonaffine motion, reproduces the pronounced plateau in the stress strain-rate flow curve as observed in laboratory measurements of steady shearing flows. We also carry out a linear stability analysis of the computed steady state solutions. The results show shear-banding in the form of sharp changes in velocity gradients, spatial variations in number density, and in alignment or stretching of the micelles. The velocity profiles obtained in numerical solutions show strong qualitative agreement with those of laboratory experiments.NSF Collaborative Research Projec

Implementation of a School-Family-Community Partnership Model to Promote Latina Youth Development: Reflections on the Process and Lessons Learned

School counselors frequently partner with families and community organizations to promote youth development and achievement. However, challenges to implementing school-family-community partnerships often preclude developing and sustaining such relationships. In this article, the authors document the implementation of a school-family-community partnership model, which was applied across two years of collaborative counseling programming for two groups of Latina youth. Semi-structured interviews with participants, parents, and educators were conducted and analyzed using qualitative content analysis to evaluate the effectiveness of the partnership and program implementation. The authors describe the outcomes of the partnership work and counseling programming as revealed by the findings, and offer reflections and lessons learned regarding the process, including implications for school counselors

Development and Persistence of 'Static' or 'Dead' Zones in Flows

Certain ceramic products are formed through extrusion processes, where a slurry is forced through small openings to form such products as filters. At the top is a large tank. The slurry is forced through the tank into an extrusion chamber, and then out through slots to form the finished product. [The slurry may be thought of as a mixture of clay (or other polymers), water, and other binders. There are many ways to model this mixture, some of which will be discussed in this report. For instance, the slurry can be modeled as a non-Newtonian fluid, a two-phase flow with liquid and solids, or a viscoelastic fluid. One can also model the mixture as an elongated particle suspension in water, where changes in the orientation of the particles could affect the flow.] After the extrusion process is complete, one finds that ‘dead zones’ of dry paste accumulate in two areas. Most prominently, they occur at the lower corners of the tank. They also occur on the floor of the extrusion chamber near the slots, both near and away from the walls. Since we will consider wall effects in the tank, for the extrusion chamber we consider only flow cells sufficiently far away from the walls. Then we may exploit the periodic nature of the device and consider only a single flow cell. The aim of this project is to determine the formation mechanisms of these dead zones, and see how they affect the overall flow

UMass Boston’s School Counseling Program at Dever-McCormack School

Given the burgeoning Latino population and the minimal research on school counseling interventions with this population, we purport to implement a culturally sensitive intervention promoting academic success among Latina youth that includes life skills, academic skills, and Latino dance

Increasing Student Achievement through School-Family-Community Collaborations: UMass Boston’s School Counseling Program at Dever School

Research has indicated that when a variety of stakeholders build partnerships, the achievement gap narrows, resulting in positive effects on student outcomes, including academic achievement, attendance, and discipline

Mitochondria directly donate their membrane to form autophagosomes during a novel mechanism of parkin-associated mitophagy

BACKGROUND: Autophagy (macroautophagy), a cellular process of “self-eating”, segregates damaged/aged organelles into vesicles, fuses with lysosomes, and enables recycling of the digested materials. The precise origin(s) of the autophagosome membrane is unclear and remains a critical but unanswered question. Endoplasmic reticulum, mitochondria, Golgi complex, and the plasma membrane have been proposed as the source of autophagosomal membranes. FINDINGS: Using electron microscopy, immunogold labeling techniques, confocal microscopy, and flow cytometry we show that mitochondria can directly donate their membrane material to form autophagosomes. We expand upon earlier studies to show that mitochondria donate their membranes to form autophagosomes during basal and drug-induced autophagy. Moreover, electron microscopy and immunogold labeling studies show the first physical evidence of mitochondria forming continuous structures with LC3-labeled autophagosomes. The mitochondria forming these structures also stain positive for parkin, indicating that these mitochondrial-formed autophagosomes represent a novel mechanism of parkin-associated mitophagy. CONCLUSIONS: With the on-going debate regarding autophagosomal membrane origin, this report demonstrates that mitochondria can donate membrane materials to form autophagosomes. These structures may also represent a novel form of mitophagy where the mitochondria contribute to the formation of autophagosomes. This novel form of parkin-associated mitophagy may be a more efficient bio-energetic process compared with de novo biosynthesis of a new membrane, particularly if the membrane is obtained, at least partly, from the organelle being targeted for later degradation in the mature autolysosome

Targeting mitochondrial oxidative stress with MitoQ reduces NET formation and kidney disease in lupus-prone MRL-lpr mice.

OBJECTIVES: Recent investigations in humans and mouse models with lupus have revealed evidence of mitochondrial dysfunction and production of mitochondrial reactive oxygen species (mROS) in T cells and neutrophils. This can provoke numerous cellular changes including oxidation of nucleic acids, proteins, lipids and even induction of cell death. We have previously observed that in T cells from patients with lupus, the increased mROS is capable of provoking oligomerisation of mitochondrial antiviral stimulator (MAVS) and production of type I interferon (IFN-I). mROS in SLE neutrophils also promotes the formation of neutrophil extracellular traps (NETs), which are increased in lupus and implicated in renal damage. As a result, in addition to traditional immunosuppression, more comprehensive treatments for lupus may also include non-immune therapy, such as antioxidants. METHODS: Lupus-prone MRL-lpr mice were treated from weaning for 11 weeks with the mitochondria-targeted antioxidant, MitoQ (200 µM) in drinking water. Mice were then assessed for ROS production in neutrophils, NET formation, MAVS oligomerisation, serum IFN-I, autoantibody production and renal function. RESULTS: MitoQ-treated mice manifested reduced neutrophil ROS and NET formation, decreased MAVS oligomerisation and serum IFN-I, and reduced immune complex formation in kidneys, despite no change in serum autoantibody . CONCLUSIONS: These findings reveal the potential utility of targeting mROS in addition to traditional immunosuppressive therapy for lupus

Future Directions for Cardiovascular Disease Comparative Effectiveness Research Report of a Workshop Sponsored by the National Heart, Lung, and Blood Institute

Comparative effectiveness research (CER) aims to provide decision makers with the evidence needed to evaluate the benefits and harms of alternative clinical management strategies. CER has become a national priority, with considerable new research funding allocated. Cardiovascular disease is a priority area for CER. This workshop report provides an overview of CER methods, with an emphasis on practical clinical trials and observational treatment comparisons. The report also details recommendations to the National Heart, Lung, and Blood Institute for a new framework for evidence development to foster cardiovascular CER, and specific studies to address 8 clinical issues identified by the Institute of Medicine as high priorities for cardiovascular CER