An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans

The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. The objective of the present work was to evaluate four methods of DNA extraction from leaves and chanar-based foods. The methods were evaluated based on yield, DNA purity, and molecular markers. The CCI-P (CTAB/Chloroform-Isoamylalcohol/pellet) method showed the highest yield of DNA obtained from leaves. However, the CPCI-SC (CTAB/Phenol-Chloroform-Isoamylalcohol/silica-column) method was the only one that resulted in acceptable DNA quality with both parameters (A260/A280 and A260/A230). The leaf DNA obtained with this method showed a greater amount of fragments with RAPD, and an acceptable amount of fragments with ISSR. On the other hand, the CCI-P method showed a higher yield of DNA from arrope de chanar (syrup). However, the CPCI-SC method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chanar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chanar-based foods

Holographic model for heavy vector meson masses

The experimentally observed spectra of heavy vector meson radial excitations show a dependence on two different energy parameters. One is associated with the quark mass and the other with the binding energy levels of the quark anti-quark pair. The first is present in the large mass of the first state while the other corresponds to the small mass splittings between radial excitations. In this article we show how to reproduce such a behavior with reasonable precision using a holographic model. In the dual picture, the large energy scale shows up from a bulk mass and the small scale comes from the position of anti-de Sitter (AdS) space where field correlators are calculated. The model determines the masses of four observed S-wave states of charmonium and six S-wave states of bottomonium with , 6.1 % rms error. In consistency with the physical picture, the large energy parameter is flavor dependent, while the small parameter, associated with quark anti-quark interaction is the same for charmonium and bottomonium states.Comment: In V5 we just added some clarifying explanations about the model. 5 tables, no figure. Version published in Europhysics Letter

Holographic Picture of Heavy Vector Meson Melting

The fraction of heavy vector mesons produced in a heavy ion collision, as compared to a proton proton collision, serves as an important indication of the formation of a thermal medium, the quark gluon plasma. This sort of analysis strongly depends on understanding the thermal effects of a medium like the plasma on the states of heavy mesons. In particular, it is crucial to know the temperature ranges where they undergo a thermal dissociation, or melting. AdS/QCD models are know to provide an important tool for the calculation of hadronic masses, but in general are not consistent with the observation that decay constants of heavy vector mesons decrease with excitation level. It has recently been shown that this problem can be overcome using a soft wall background and introducing an extra energy parameter, through the calculation of correlation functions at a finite position of anti-de Sitter space. This approach leads to the evaluation of masses and decay constants of S wave quarkonium states with just one flavor dependent and one flavor independent parameters. Here we extend this more realistic model to finite temperatures and analyse the thermal behavior of the states $1S, 2S$ and $3S$ of bottomonium and charmonium. The corresponding spectral function exhibits a consistent picture for the melting of the states where, for each flavor, the higher excitations melt at lower temperatures. We estimate for these six states, the energy ranges in which the heavy vector mesons undergo a transition from a well defined peak in the spectral function to complete melting in the thermal medium. A very clear distinction between the heavy flavors emerges, with bottomonium state $\Upsilon (1S)$ surviving deconfinemet transition at temperatures much larger than the critical deconfinement temperature of the medium.Comment: 20 pages, 7 figure

Decay constants in soft wall AdS/QCD revisited

Phenomenological AdS/QCD models, like hard wall and soft wall, provide hadronic mass spectra in reasonable consistency with experimental and (or) lattice results. These simple models are inspired in the AdS/CFT correspondence and assume that gauge/ gravity duality holds in a scenario where conformal invariance is broken through the introduction of an energy scale. Another important property of hadrons: the decay constant, can also be obtained from these models. However, a consistent formulation of an AdS/QCD model that reproduces the observed behavior of decay constants of vector meson excited states is still lacking. In particular: for radially excited states of heavy vector mesons, the experimental data lead to decay constants that decrease with the radial excitation level. We show here that a modified framework of soft wall AdS/QCD involving an additional dimensionfull parameter, associated with an ultraviolet energy scale, provides decay constants decreasing with radial excitation level. In this version of the soft wall model the two point function of gauge theory operators is calculated at a finite position of the anti-de Sitter space radial coordinate.Comment: Shorter (letter) version. Results unchanged. More references included. We now explain that the large UV scale of the model is associated with the non-hadronic decay of the heavy vector meson into light leptons. Version Published in Phys. Lets.

Bulge RR Lyrae stars in the VVV tile b201\textit{b201}

The VISTA Variables in the V\'ia L\'actea (VVV) Survey is one of the six ESO public surveys currently ongoing at the VISTA telescope on Cerro Paranal, Chile. VVV uses near-IR ($ZYJHK_{\rm s}$) filters that at present provide photometry to a depth of $K_{\rm s} \sim 17.0$ mag in up to 36 epochs spanning over four years, and aim at discovering more than 10$^6$ variable sources as well as trace the structure of the Galactic bulge and part of the southern disk. A variability search was performed to find RR Lyrae variable stars. The low stellar density of the VVV tile $\textit{b201}$, which is centered at ($\ell, b$) $\sim$ ($-9^\circ, -9^\circ$), makes it suitable to search for variable stars. Previous studies have identified some RR Lyrae stars using optical bands that served to test our search procedure. The main goal is to measure the reddening, interstellar extinction, and distances of the RR Lyrae stars and to study their distribution on the Milky Way bulge. A total of 1.5 sq deg were analyzed, and we found 39 RR Lyrae stars, 27 of which belong to the ab-type and 12 to the c-type. Our analysis recovers all the previously identified RR Lyrae variables in the field and discovers 29 new RR Lyrae stars. The reddening and extinction toward all the RRab stars in this tile were derived, and distance estimations were obtained through the period--luminosity relation. Despite the limited amount of RR Lyrae stars studied, our results are consistent with a spheroidal or central distribution around $\sim 8.1$ and $\sim 8.5$ kpc. for either the Cardelli or Nishiyama extinction law.Comment: 10 pages, 8 figures, accepted for publication in Astronomy and Astrophysic

Radiative non-isothermal Bondi accretion onto a massive black hole

In this paper, we present the classical Bondi accretion theory for the case of non-isothermal accretion processes onto a supermassive black hole (SMBH), including the effects of X-ray heating and the radiation force due to electron scattering and spectral lines. The radiation field is calculated by considering an optically thick, geometrically thin, standard accretion disk as the emitter of UV photons and a spherical central object as a source of X-ray emission. In the present analysis, the UV emission from the accretion disk is assumed to have an angular dependence, while the X-ray/central object radiation is assumed to be isotropic. This allows us to build streamlines in any angular direction we need to. The influence of both types of radiation is evaluated for different flux fractions of the X-ray and UV emissions with and without the effects of spectral line driving. We find that the radiation emitted near the SMBH interacts with the infalling matter and modifies the accretion dynamics. In the presence of line driving, a transition resembles from pure type 1 & 2 to type 5 solutions (see Fig2.1 of Frank etal. 2002), which takes place regardless of whether or not the UV emission dominates over the X-ray emission. We compute the radiative factors at which this transition occurs, and discard type 5 solution from all our models. Estimated values of the accretion radius and accretion rate in terms of the classical Bondi values are also given. The results are useful for the construction of proper initial conditions for time-dependent hydrodynamical simulations of accretion flows onto SMBH at the centre of galaxies.Comment: 10 pages, 10 figures, Accepted to be published in A&

Variable stars in Terzan 5: additional evidence of multi-age and multi-iron stellar populations

Terzan 5 is a complex stellar system in the Galactic bulge, harboring stellar populations with very different iron content ({\Delta}[Fe/H] ~1 dex) and with ages differing by several Gyrs. Here we present an investigation of its variable stars. We report on the discovery and characterization of three RR Lyrae stars. For these newly discovered RR Lyrae and for six Miras of known periods we provide radial velocity and chemical abundances from spectra acquired with X-SHOOTER at the VLT. We find that the three RR Lyrae and the three short period Miras (P<300 d) have radial velocity consistent with being Terzan 5 members. They have sub-solar iron abundances and enhanced [{\alpha}/Fe], well matching the age and abundance patterns of the 12 Gyr metal-poor stellar populations of Terzan 5. Only one, out of the three long period (P>300 d) Miras analyzed in this study, has a radial velocity consistent with being Terzan 5 member. Its super-solar iron abundance and solar-scaled [{\alpha}/Fe] nicely match the chemical properties of the metal rich stellar population of Terzan 5 and its derived mass nicely agrees with being several Gyrs younger than the short period Miras. This young variable is an additional proof of the surprising young sub-population discovered in Terzan 5.Comment: 20 pages, 4 figures, in press on the Ap

New type II Cepheids from VVV data towards the Galactic center

The Galactic center (GC) is the densest region of the Milky Way. Variability surveys towards the GC potentially provide the largest number of variable stars per square degree within the Galaxy. However, high stellar density is also a drawback due to blending. Moreover, the GC is affected by extreme reddening, therefore near infrared observations are needed. We plan to detect new variable stars towards the GC, focusing on type II Cepheids (T2Cs) which have the advantage of being brighter than RR Lyrae stars. We perform parallel Lomb-Scargle and Generalized Lomb-Scargle periodogram analysis of the $K_s$-band time series of the VISTA variables in the Via Lactea survey, to detect periodicities. We employ statistical parameters to clean our sample. We take account of periods, light amplitudes, distances, and proper motions to provide a classification of the candidate variables. We detected 1,019 periodic variable stars, of which 164 are T2Cs, 210 are Miras and 3 are classical Cepheids. We also found the first anomalous Cepheid in this region. We compare their photometric properties with overlapping catalogs and discuss their properties on the color-magnitude and Bailey diagrams. We present the most extensive catalog of T2Cs in the GC region to date. Offsets in E($J-K_s$) and in the reddening law cause very large ($\sim$1-2 kpc) uncertainties on distances in this region. We provide a catalog which will be the starting point for future spectroscopic surveys in the innermost regions of the Galaxy.Comment: A&A, accepte

Poisson smooth structures on stratified symplectic spaces

In this paper we introduce the notion of a smooth structure on a stratified space, the notion of a Poisson smooth structure and the notion of a weakly symplectic smooth structure on a stratified symplectic space, refining the concept of a stratified symplectic Poisson algebra introduced by Sjamaar and Lerman. We show that these smooth spaces possess several important properties, e.g. the existence of smooth partitions of unity. Furthermore, under mild conditions many properties of a symplectic manifold can be extended to a symplectic stratified space provided with a smooth Poisson structure, e.g. the existence and uniqueness of a Hamiltonian flow, the isomorphism between the Brylinski-Poisson homology and the de Rham homology, the existence of a Leftschetz decomposition on a symplectic stratified space. We give many examples of stratified symplectic spaces possessing a Poisson smooth structure which is also weakly symplectic.Comment: 21 page, final version, to appear in the Proceedings of the 6-th World Conference on 21st Century Mathematic