Management and Modeling of Winter-time Basil Cultivars Grown with a Cap MAT System

Basil (Ocimum basilicum) is a high value crop, currently grown in the field and greenhouses in Nebraska. Winter-time, greenhouse studies were conducted during 2015 and 2016, focusing on cultivars of basil grown on a Cap MAT II® system with various levels of fertilizer application. The goal was to select high value cultivars that could be grown in Nebraska greenhouses. The studies used water content, electrical conductivity, photosynthetically active radiation (PAR), and relative humidity, air and soil media temperature sensors. Greenhouse systems can be very complex, even though controlled by mechanical heating and cooling. Uncertain or ambiguous environmental and plant growth factors can occur, where growers need to plan, adapt, and react appropriately. Plant harvest weights and electronic sensor data was recorded over time and used for training and internally validating fuzzy logic inference and classification models. Studies showed that GENFIS2 ‘subtractive clustering’ of data, prior to ANFIS training, resulted in good correlations for predicted growth (R2 \u3e 0.85), with small numbers of effective rules and membership functions. Cross-validation and internal validation studies also showed good correlations (R2 \u3e 0.85). Decisions on basil cultivar selection and forecasting as to how quickly a basil crop will reach marketable size will help growers to know when to harvest, for optimal yield and predictable quantity of essential oils. If one can predict reliably how much essential oil will be produced, then the methods and resultant products can be proposed for USP or FDA approval. Currently, most plant medicinal and herbal oils and other supplements vary too widely in composition for approval. The use of fuzzy set theory could be a useful mathematical tool for plant and horticultural production studies

Management and Modeling of Winter-time Basil Cultivars Grown with a Cap MAT System

Basil (Ocimum basilicum) is a high value crop, currently grown in the field and greenhouses in Nebraska. Winter-time, greenhouse studies were conducted during 2015 and 2016, focusing on cultivars of basil grown on a Cap MAT II® system with various levels of fertilizer application. The goal was to select high value cultivars that could be grown in Nebraska greenhouses. The studies used water content, electrical conductivity, photosynthetically active radiation (PAR), and relative humidity, air and soil media temperature sensors. Greenhouse systems can be very complex, even though controlled by mechanical heating and cooling. Uncertain or ambiguous environmental and plant growth factors can occur, where growers need to plan, adapt, and react appropriately. Plant harvest weights and electronic sensor data was recorded over time and used for training and internally validating fuzzy logic inference and classification models. Studies showed that GENFIS2 ‘subtractive clustering’ of data, prior to ANFIS training, resulted in good correlations for predicted growth (R2 \u3e 0.85), with small numbers of effective rules and membership functions. Cross-validation and internal validation studies also showed good correlations (R2 \u3e 0.85). Decisions on basil cultivar selection and forecasting as to how quickly a basil crop will reach marketable size will help growers to know when to harvest, for optimal yield and predictable quantity of essential oils. If one can predict reliably how much essential oil will be produced, then the methods and resultant products can be proposed for USP or FDA approval. Currently, most plant medicinal and herbal oils and other supplements vary too widely in composition for approval. The use of fuzzy set theory could be a useful mathematical tool for plant and horticultural production studies

Storage and breakdown of starch aid \u3cem\u3eP. parviflorus\u3c/em\u3e in leaf re-greening after nitrogen deficiency

Plectranthus parviflorus, common Swedish ivy does not lose leaves when it is deprived of nitrogen. Instead this plant retains its yellow leaves and upon reintroduction of nitrogen will re-green and start to grow. In two experiments, rooted cuttings of common Swedish ivy were grown with (150 ppm N) and without nitrogen for 3 weeks. After some plants were sampled the others were either switched or kept at 0 or 150 ppm N and allowed to grow for another 3 weeks. After another sampling, plants were again switched or kept at 0 or 150 ppm N for a final 3 weeks. At each harvest, leaves were tested for starch, sampled for microscopy and then dried and weighed for soluble carbohydrate extraction. Data collected indicates that yellow leaves store and breakdown starch into soluble carbohydrates (specifically reducing sugars) in order to keep leaves from senescing. When nitrogen is re-supplied to these plants, leaves re-green and the plant continues to grow. We propose that common Swedish ivy’s ability to store and breakdown starch aids in the process of leaf re-greening

Educating Future Nursing Scientists: Recommendations for Integrating Omics Content in PhD Programs

Preparing the next generation of nursing scientists to conduct high-impact, competitive, sustainable, innovative, and interdisciplinary programs of research requires that the curricula for PhD programs keep pace with emerging areas of knowledge and health care/biomedical science. A field of inquiry that holds great potential to influence our understanding of the underlying biology and mechanisms of health and disease is omics. For the purpose of this article, omics refers to genomics, transcriptomics, proteomics, epigenomics, exposomics, microbiomics, and metabolomics. Traditionally, most PhD programs in schools of nursing do not incorporate this content into their core curricula. As part of the Council for the Advancement of Nursing Science\u27s Idea Festival for Nursing Science Education, a work group charged with addressing omics preparation for the next generation of nursing scientists was convened. The purpose of this article is to describe key findings and recommendations from the work group that unanimously and enthusiastically support the incorporation of omics content into the curricula of PhD programs in nursing. The work group also calls to action faculty in schools of nursing to develop strategies to enable students needing immersion in omics science and methods to execute their research goals

Strawberry cultivars vary in productivity, sugars and phytonutrient content when grown in a greenhouse during the winter

In many areas of the US, fresh locally grown berries are not available during the winter. With this in mind, a research study comprised of three experiments was conducted focused on cultivar selection for berry yield, number, sweetness and phytonutrient content. Using a capillary mat system with under bench heating within a double-layer polyethylene greenhouse, strawberries were grown in the Great Plains Region of the US during the winter. During experiment 1, 12 cultivars were grown; berries were weighed, counted and analyzed for sugars and phytonutrients. “Albion” plants produced a high number/mass of berries, had relatively high sugar content but a lower level of phytonutrients when compared to other cultivars. Sugar and phytonutrients concentrations overlapped across cultivars and thus, one cultivar could not be statistically singled out as best. As all cultivars flowered and fruited, two additional 8-month-long experiments were conducted. It took only 7 weeks from potting of dormant crowns for most cultivars to produce fruit. Certain cultivars fruited more successfully during certain months than others, but this was not associated with response time. For example,” “Albion”, “Chandler”, “Darselect”, “Evie-2” and “Seascape” plants consistently produced fruit October to early January while “AC Wendy”, “Cavendish”, “Honeoye” and “Strawberry Festival” plants mainly produced berries in March/April. Summed over experiment 2, “Albion”, “ Cavendish”, “Chandler”, “ Evie-2”, “Portola” and “Seascape” plants produced the greatest mass of berries. “AC Wendy” and “Darselect” berries contained some of the highest levels of sugars while berries from “Chandler”, “Darselect”, “Evie-2”, “Seascape” and “Strawberry Festival” had some of the highest phytonutrient values. In the third experiment, of the 8 selected cultivars, “Evie-2”, “Evie−2+” and “Portola” plants had the highest total yield and average berry mass/plant. “Seascape” and” Chandler” plants were second in total production. Glucose, fructose and sucrose levels varied across cultivars with “Chandler” and “Seascape” berries possessing the lowest level of total sugars. Phytonutrient values varied among cultivars with some having better flavonoids (“Seascape”), phenols (“Seascape” and “Chandler”) and ant oxidant capacity (“Seascape”, “Evie-2” and “Cavendish”). Measurement of soluble solids concentration varied by week among the cultivars with “Seascape”, “Seascape+”, and “Albion” berries possessing higher levels than other cultivars such as “Cavendish”. Overall, under these winter greenhouse conditions using capillary mat fertigation and an under-bench heat delivery system, strawberries were successfully produced for the off-season market

Comparison of Winter Strawberry Production in a Commercial Heated High Tunnel versus a University Greenhouse

For the past 4 years, the University of Nebraska strawberry team has worked to develop low cost, sustainable methods for farmers and growers to produce strawberries in a double polyethylene greenhouse during the winter. This past year, this growing system was adapted to become a commercial grower’s heated high tunnel for the winter/spring of 2013-14. The idea was to scale up to a farm-size demonstration and compare it to the university greenhouse production system with a goal to expand marketing opportunities for strawberries into the winter season

Prevalence, incidence, and clearance of human papillomavirus types covered by current vaccines in men with human immunodeficiency virus in the SUN Study

BACKGROUND: High-risk anal human papillomavirus (HPV) infection is prevalent among men living with human immunodeficiency virus (HIV); the association between 9-valent (9v) high-risk HPV (HR-HPV) vaccine types and abnormal cytology has not been well characterized. METHODS: We followed a prospective cohort study of persons with HIV at 7 HIV clinics in 4 US cities from March 2004 through June 2012. Annually, providers collected separate anal swabs for HPV detection and cytopathologic examination. Among men, we examined prevalence, incidence, and clearance of 9v HR-HPV vaccine types, compared with other HR types, and associations with abnormal cytology to assess potential vaccine impact. RESULTS: Baseline prevalence of any anal 9v HR-HPV type among men who have sex with men (MSM) and men who have sex with women (MSW) was 74% and 25% (P \u3c .001), respectively. Among 299 MSM, abnormal cytology was detected in 161 (54%) MSM and was associated with the presence of any 9v HR-HPV (relative risk [RR], 1.8 [95% confidence interval {CI}, 1.3-2.6]; P \u3c .001). Among 61 MSW, abnormal anal cytology was detected in 12 (20%) and was associated with the presence of any 9v HR-HPV (RR, 4.3 [95% CI, 1.6-11.5]; P \u3c .001). CONCLUSIONS: Among men with HIV, the prevalence of the 7 HR-HPV types in the 9v vaccine was high and was associated with abnormal cytology. These findings indicate that men with HIV could benefit from prophylactic administration of the 9v HPV vaccine

Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator

Male gender is protective against multiple sclerosis and other T-cell-mediated autoimmune diseases. This protection may be due, in part, to higher androgen levels in males. Androgen binds to the androgen receptor (AR) to regulate gene expression, but how androgen protects against autoimmunity is not well understood. Autoimmune regulator (Aire) prevents autoimmunity by promoting self-antigen expression in medullary thymic epithelial cells, such that developing T cells that recognize these self-antigens within the thymus undergo clonal deletion. Here we show that androgen upregulates Aire-mediated thymic tolerance to protect against autoimmunity. Androgen recruits AR to Aire promoter regions, with consequent enhancement of Aire transcription. In mice and humans, thymic Aire expression is higher in males compared with females. Androgen administration and male gender protect against autoimmunity in a multiple sclerosis mouse model in an Aire-dependent manner. Thus, androgen control of an intrathymic Aire-mediated tolerance mechanism contributes to gender differences in autoimmunity

Prevalence, incidence, and clearance of anal high-risk human papillomavirus infection among HIV-infected men in the SUN study

BACKGROUND: The natural history of anal human papilloma virus (HPV) infection among human immunodeficiency virus (HIV)-infected men is unknown. METHODS: Annually, from 2004 to 2012, we examined baseline prevalence, incidence, and clearance of anal HPV infection at 48 months, and associated factors among HIV-infected men. RESULTS: We examined 403 men who have sex with men (MSM) and 96 men who have sex with women (MSW) (median age 42 years for both, 78% versus 81% prescribed cART, median CD4+ T-lymphocyte cell count 454 versus 379 cells/mm3, and 74% versus 75% had undetectable viral load, respectively). Type 16 prevalence among MSM and MSW was 38% versus 14% (P \u3c .001), and incidence 24% versus 7% (P = .001). Type 18 prevalence was 24% versus 8% (P \u3c .001), and incidence 13% versus 4% (P = .027). Among MSM and MSW, clearance of prevalent HPV 16 and HPV 18 was 31% and 60% (P = .392), and 47% and 25% (P = .297), respectively. Among MSM, receptive anal sex (with or without a condom) was associated with persistent HPV 16 (OR 2.24, P \u3c .001). CONCLUSIONS: MSM had higher prevalence and incidence of HPV than MSW, but similar clearance. Receptive anal sex may predict cancer risk among HIV-infected MSM