Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress

Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3¿-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed map

The radiological report... and then what?

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Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant

© Gramazio et al.; licensee BioMed Central. 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

Modelling system dynamics as a socio-ecological perspective to support human-beaver interactions

Beavers are semi-aquatic mammals that significantly impact on freshwater ecosystems, creating benefits and challenges, particularly in areas with close human interaction. Managing human-beaver interactions is a multifaceted issue due to the many variables involved, but the complexity of these interactions can be analysed effectively using system dynamics models. These models are used in many contexts, including wildlife management, to simulate a variety of management policies and assess their effects. The present study addressed gaps in the literature by developing a system dynamics model that examined both the benefits and conflicts that arise from human-beaver interactions. The model, implemented using Vensim PLE software, synthesised qualitative and quantitative data to simulate four simulation scenarios: ecological, social, economic, and policy making. The study examined the dynamics of beaver populations, ecosystem service provision, social perceptions, and management strategies in a case study of the Ivrea lakes area in the Piedmont region (Italy). Model predictions highlights that beaver populations stabilized logistically, influencing ecosystem services and residual capital, while social acceptance stongly reduced management costs and social pressure. Optimal budget allocation and combined strategies emerged as key to sustainable management and conflict mitigation. Overall, predictions suggest that an integrated approach that prioritises prevention and actively engages local communities can improve both ecological outcomes and social acceptance of beavers. The model is a useful decision and discussion tool for assessing management strategies and facilitating stakeholder involvement. Future studies should expand on these results by exploring additional beaver-related conflicts and benefits in diverse contexts

Vertical Greening Systems: A Critical Comparison of Do-It-Yourself Designs

Due to the increasing shortage of space in urban areas, vertical greening systems (VGSs) are becoming increasingly popular as a means to provide increased urban greening using building facades. VGSs are usually installed and managed by experts due to technical complexity, however the role of local communities is becoming increasingly important through Do-It-Yourself (DIY) practices. This study aims to explore low-cost VGSs and provide design suggestions and maintenance indications to encourage the expanded use of in situ small-scale VGSs. Firstly, an exploratory review of VGS designs proposed in the scientific literature, and by commercial and community-based solutions was conducted taking DIY potential into account to define eight basic design models categorized through six structural criteria. Then, seven community garden groups were interviewed to inform a critical comparison of the eight design models. Data collected was synthesized to develop a star rating system, thus providing a quick comparative tool. The star rating system shows the performance of five relevant DIY design parameters for each VGS model. The current research may assist in the accessibility of green technologies and facilitate community-scale implementation of DIY vertical greening

Application of the socio-ecological system framework to forest fire risk management: A systematic literature review

Although increasing concern about climate change has raised awareness of the fundamental role of forest ecosystems, forests are threatened by human-induced impacts worldwide. Among them, wildfire risk is clearly the result of the interaction between human activities, ecological domains, and climate. However, a clear understanding of these interactions is still needed both at the global and local levels. Numerous studies have proven the validity of the socioecological system (SES) approach in addressing this kind of interdisciplinary issue. Therefore, a systematic review of the existing literature on the application of SES frameworks to forest ecosystems is carried out, with a specific focus on wildfire risk management. The results demonstrate the existence of different methodological approaches that can be grouped into seven main categories, which range from qualitative analysis to quantitative spatially explicit investigations. The strengths and limitations of the approaches are discussed, with a specific reference to the geographical setting of the works. The research suggests the importance of local community involvement and local knowledge consideration in wildfire risk management. This review provides a starting point for future research on forest SES and a supporting tool for the development of a sustainable wildfire risk adaptation and mitigation strategy

The reintroduction of Castor fiber in Piedmont (Italy): An integrated SWOT-spatial multicriteria based approach for the analysis of suitability scenarios

The Castor fiber or Eurasian beaver can change its habitat by building dams and creating ponds. For this reason, Castor fiber is known as an “ecosystem engineer” for aquatic and riparian environments. Despite its ecological importance, at the beginning of the 20th century the population was reduced to only 1200 beavers in Europe and Asia, due to uncontrolled hunting. Recently, some reintroductions and translocations have partly re-established the population. In Italy, however, the beaver disappeared in the 16th century and no action has been taken despite the recommendation of the Council of Europe to perform a feasibility study. This research evaluates beaver reintroduction and identifies suitable areas in Italy and, in particular, in the Piedmont region. In order to achieve this, a SWOT analysis combined with a Spatial Multicriteria Analysis was performed. Firstly, the zoological and ethological aspects concerning this rodent were studied, as well as the historical reasons that led to its disappearance in Italy and near extinction in Eurasia. Secondly, Strengths, Weaknesses, Opportunities and Threats (SWOT) of the territory were identified for beaver reintroduction. The SWOT analysis was implemented, as the starting point for the spatial multicriteria analysis. Thirdly, the Multicriteria Spatial Decision Support System (MC-SDSS) was structured into two criteria, i.e. Potentials and Criticalities, representing the spatialization of strengths and weaknesses. The final result of the MC-SDSS is a map showing suitable areas for beaver reintroduction in Piedmont. This map is the weighted sum of the maps of criticalities and potentialities, performed through a set of GIS operations and weighted through a pairwise comparison of criteria by experts. The analysis was conducted for the Piedmont region, but the integrated approach and the set of criteria can also be applied in other regions. Moreover, this mixed-method approach takes into account the characteristics necessary for the choice of suitable beaver habitats and also includes economic and social aspects. Therefore, it is an improvement on the Habitat Suitability Index (HIS), generally used in reintroductions. The aspects considered in the analysis are fundamental for the future development of a shared action plan, which considers both technical and social motivations and acts for the long-term on a wide area

A chromosome-anchored eggplant genome sequence reveals key events in Solanaceae evolution

With approximately 450 species, spiny Solanum species constitute the largest monophyletic group in the Solanaceae family, but a high-quality genome assembly from this group is presently missing. We obtained a chromosome-anchored genome assembly of eggplant (Solanum melongena), containing 34,916 genes, confirming that the diploid gene number in the Solanaceae is around 35,000. Comparative genomic studies with tomato (S. lycopersicum), potato (S. tuberosum) and pepper (Capsicum annuum) highlighted the rapid evolution of miRNA:mRNA regulatory pairs and R-type defense genes in the Solanaceae, and provided a genomic basis for the lack of steroidal glycoalkaloid compounds in the Capsicum genus. Using parsimony methods, we reconstructed the putative chromosomal complements of the key founders of the main Solanaceae clades and the rearrangements that led to the karyotypes of extant species and their ancestors. From 10% to 15% of the genes present in the four genomes were syntenic paralogs (ohnologs) generated by the pre-γ, γ and T paleopolyploidy events, and were enriched in transcription factors. Our data suggest that the basic gene network controlling fruit ripening is conserved in different Solanaceae clades, and that climacteric fruit ripening involves a differential regulation of relatively few components of this network, including CNR and ethylene biosynthetic genes

Associations between rapid weight gain in infancy and weight status among urban Aboriginal children participating in the Gudaga study: nine-year results from a cohort study.

BACKGROUND:Rapid weight gain (RWG) in infants is associated with overweight and obesity in childhood and beyond, highlighting the need for early intervention. METHODS:Data from a birth cohort of Australian Aboriginal and Torres Strait Islander children living in an urban area were analysed to determine the prevalence of RWG in infancy and the association between RWG and overweight and obesity, categorised using both body mass index and waist to height ratio from birth to 9 years. RESULTS:The prevalence of overweight and obesity is higher in this cohort (at 47%) than the population average. The Australian population as a whole has seen steady increases. In this cohort although the prevalence of combined overweight and obesity remained relatively stable between 2 and 9 years, the proportion of children categorized as obese using BMI has increased. 42% of children who were overweight or obese at 9 years had experienced RWG in infancy. Children were 2.7 and 3.9 times more likely to be overweight at 9 years if they experienced RWG or were overweight at 2 years, respectively. CONCLUSION:RWG was common in this cohort and the strongest predictor of excess weight at 2 years and at 9 years. Early intervention is crucial in the first year of life across the whole population to prevent obesity in children. Culturally appropriate interventions developed with the community are required for Aboriginal and Torres Strait Islander babies and their parents