Vegetation recolonisation of abandoned agricultural terraces on Antikythera, Greece

Antikythera is a small, relatively remote Mediterranean island, lying 35 km north-west of Crete, and its few contemporary inhabitants live mainly in the small village at the only port. However, an extensive network of terraces across the island bears witness to the past importance of farming on the island, although the intensity of use of these cultivated plots has changed according to fluctuating population levels. Most recently, the rural population and intensity of cultivation have dramatically declined. Our aim is to understand the recolonisation process of agricultural land by plants after terraces are no longer used for the cultivation of crops. The results demonstrate a relatively quick pace of vegetative recolonisation, with abandoned farm land covered by dense scrub within 20 to 60 years. The archaeological implications are that, following even relatively short periods of abandonment, the landscape would have required arduous reinvestment in the removal of scrub growth, as well as the repair and construction of stone terraces, to allow cultivation once again

Wild plant use in European Neolithic subsistence economies: a formal assessment of preservation bias in archaeobotanical assemblages and the implications for understanding changes in plant diet breadth

In this paper we estimate the degree to which the range and proportion of wild plant foods are under-represented in samples of charred botanical remains from archaeological sites. We systematically compare the differences between central European Neolithic archaeobotanical assemblages that have been preserved by charring compared to those preserved by waterlogging. Charred archaeobotanical assemblages possess on aggregate about 35% of the range of edible plants documented in waterlogged samples from wetland settlements. We control for the ecological availability of wetland versus terrestrial wild plant foods on assemblage composition and diversity, and demonstrate that the significantly broader range of wild plant food taxa represented is primarily a function of preservation rather than subsistence practices. We then consider whether observed fluctuations in the frequency of edible wild taxa over time can also be attributed to preservation, and demonstrate that it cannot; and thus conclude that there are significant changes in plant food diets during the Neolithic that reflect different strategies of land use and, over time, a decreasing reliance on foraging for wild plant foods. The wild species included in our analyses are not spatially restricted—they are common throughout central Europe. We maintain, therefore, that our results are relevant beyond our study area and more generally illustrate the challenges of attempting to reconstruct the relative importance of wild plant foods—and thus plant diet breadth—in Neolithic archaeobotanical assemblages from charred data alone

Profile and correlates of injecting-related injuries and diseases among people who inject drugs in Australia

Introduction: People who inject drugs (PWID) commonly experience harms related to their injecting, many of which are consequences of modifiable drug use practices. There is currently a gap in our understanding of how certain injecting-related injuries and diseases (IRID) cluster together, and socio-demographic and drug use characteristics associated with more complex clinical profiles. Method: Surveys were conducted with 902 Australian PWID in 2019. Participants provided information regarding their drug use, and past month experience of the following IRID: artery injection, nerve damage, skin and soft tissue infection, thrombophlebitis, deep vein thrombosis, endocarditis, septic arthritis, osteomyelitis, and septicaemia. We performed a latent class analysis, grouping participants based on reported IRID and ran a class-weighted regression analysis to determine variables associated with class-membership. Results: One-third (34 %) of the sample reported any IRID. A 3-class model identified: 1) no IRID (73 %), moderate IRID (21 %), and 3) high IRID (6%) clusters. Re-using one`s own needles was associated with belonging to the high IRID versus moderate IRID class (ARRR = 2.38; 95 % CI = 1.04−5.48). Other factors, including daily injecting and past 6-month mental health problems were associated with belonging to moderate and high IRID classes versus no IRID class. Conclusion: A meaningful proportion of PWID reported highly complex IRID presentations distinguished by the presence of thrombophlebitis and associated with greater re-use of needles. Increasing needle and syringe coverage remains critical in addressing the harms associated with injecting drug use and expanding the capacity of low-threshold services to address less severe presentations might aid in reducing IRID amongst PWID

The origins and spread of stock-keeping: the role of cultural and environmental influences on early Neolithic animal exploitation in Europe

It has long been recognised that the proportions of Neolithic domestic animal species—cattle, pig and sheep/goat—vary from region to region, but it has hitherto been unclear how much this variability is related to cultural practices or to environmental constraints. This study uses hundreds of faunal assemblages from across Neolithic Europe to reveal the distribution of animal use between north and south, east and west. The remarkable results present us with a geography of Neolithic animal society—from the rabbit-loving Mediterranean to the beef-eaters of the north and west. They also demonstrate that the choices made by early Neolithic herders were largely determined by their environments. Cultural links appear to have played only a minor role in the species composition of early Neolithic animal societie

Correction to: Consent requirements for research with human tissue: Swiss ethics committee members disagree.

It has come to our attention that in the original article [1] information regarding dates was omitted. The data in this study were obtained in Switzerland four years before the entering into force of the new Swiss Human Research Act in 2014, when the guidelines of the Swiss Academy of Medical Sciences (SAMS) ceased to apply. It is important for readers to know that at the time of the study there was no binding law in Switzerland, only the more open SAMS guidelines that have a different legal status. We would expect to find less variation of opinions among research ethics committee members if the study were repeated after the federal law came into force

Domestication as innovation : the entanglement of techniques, technology and chance in the domestication of cereal crops

The origins of agriculture involved pathways of domestication in which human behaviours and plant genetic adaptations were entangled. These changes resulted in consequences that were unintended at the start of the process. This paper highlights some of the key innovations in human behaviours, such as soil preparation, harvesting and threshing, and how these were coupled with genetic ‘innovations’ within plant populations. We identify a number of ‘traps’ for early cultivators, including the needs for extra labour expenditure on crop-processing and soil fertility maintenance, but also linked gains in terms of potential crop yields. Compilations of quantitative data across a few different crops for the traits of nonshattering and seed size are discussed in terms of the apparently slow process of domestication, and parallels and differences between different regional pathways are identified. We highlight the need to bridge the gap between a Neolithic archaeobotanical focus on domestication and a focus of later periods on crop-processing activities and labour organization. In addition, archaeobotanical data provide a basis for rethinking previous assumptions about how plant genetic data should be related to the origins of agriculture and we contrast two alternative hypotheses: gradual evolution with low selection pressure versus metastable equilibrium that prolonged the persistence of ‘semi-domesticated’ populations. Our revised understanding of the innovations involved in plant domestication highlight the need for new approaches to collecting, modelling and integrating genetic data and archaeobotanical evidence

Intra-amniotic delivery of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred CFTR-knockout mice

This article is available open access through the publisher’s website at the link below. Copyright © 2008 The American Society of Gene Therapy.Due to its early onset and severe prognosis, cystic fibrosis (CF) has been suggested as a candidate disease for in utero gene therapy. In 1997, a study was published claiming that to how transient prenatal expression of CF transmembrane conductance regulator (CFTR) from an in utero –injected adenovirus vector could achieve permanent reversal of the CF intestinal pathology in adult CF knockout mice, despite the loss of CFTR transgene expression by birth. This would imply that the underlying cause of CF is a prenatal defect for which lifelong cure can be achieved by transient prenatal expression of CFTR. Despite criticism at the time of publication, no independent verification of this contentious finding has been published so far. This is vital for the development of future therapeutic strategies as it may determine whether CF gene therapy should be performed prenatally or postnatally. We therefore reinvestigated this finding with an identical adenoviral vector and a knockout CF mouse line (CftrtmlCam) with a completely inbred genetic background to eliminate any effects due to genetic variation. After delivery of the CFTR-expressing adenovirus to the fetal mouse, both vector DNA and transgenic CFTR expression were detected in treated animals postpartum but statistically no significant difference in survival was observed between the Cftr–/– mice treated with the CFTR-adenovirus and those treated with the control vector.Sport Aiding Medical Research for Kids, the Cystic Fibrosis Trust, and the Katharine Dormandy Trust