Long continuously chirped fibre Bragg gratings for compensation of linear- and 3rd order-dispersion

For the first time long broadband chirped fibre Bragg gratings with a dispersion profile designed to compensate 3rd order-dispersion are presented. These results demonstrate how the increased demands for dispersion compensation at very high bit-rates can be met using chirped fibre Bragg grating

Optimisation of DSF and SOA based phase conjugators by incorporating noise-suppressing fibre gratings

We compare the performance of dispersion-shifted-fibre (DSF) and semiconductor-optical-amplifier (SOA) based phase conjugators for a 10 Gb/s non-return-to-zero system with respect to conversion efficiency, noise figure and distortion. Fibre gratings are used for signal extraction and amplified spontaneous emission (ASE) suppression, allowing closer wavelength spacing and reducing the conjugation noise figure by up to 12 dB. Despite the higher SOA conversion efficiency, both conjugators give similar noise figures with ASE suppression. However, the DSF based conjugator has the advantage of distortion tolerance at higher input power. Introduction: Optical phase conjugation has attracted much recent research attention due to its potential application for group-velocity-dispersion and self-phase-modulation compensation in mid-point spectral inversion (MPSI) systems, and also for coherent wavelength conversion in optical switching and routing. The two most promising optical phase conjugation techniques are four-wave mixing (FWM) in either dispersion-shifted fibre (DSF), or semiconductor optical amplifiers (SOA). A DSF based conjugator requires phase matching close to its zero dispersion wavelength for efficient four-wave mixing. This restricts its wavelength flexibility compared to an SOA based conjugator which offers a much wider conversion bandwidth. Furthermore, the low FWM conversion efficiency in passive DSF seems to make the SOA a preferred phase conjugating medium. However, in a practical communication system, conjugation optical signal-to-noise ratio (SNR) is more important than conversion efficiency. The noise at the conjugate wavelength is usually dominated by the amplified spontaneous emission (ASE) noise from the pump and signal. The reduction of this noise has been demonstrated in an SOA based conjugator (i) by bandpass filtering of the pump and/or signal waves before mixing, and (ii) by the insertion of a notch filter at the conjugate wavelength before the conjugator. In this letter, SOA and DSF based conjugators are compared by investigating the conversion efficiency, noise and eye opening in a 10 Gb/s non-return-to-zero (NRZ) externally-modulated system, using an identical filtering network. We report for the first time the use of fibre gratings for efficient ASE noise filtering and conjugate signal extraction. The performance enhancement using these noise-suppressing gratings is also investigated

Fibre optic sensors for high speed hypervelocity impact studies and low velocity drop tests

The initial aim of this project was to develop a non-contact fibre optic based displacement sensor to operate in the harsh environment of a 'Light Gas Gun' (LGG), which can 'fire' small particles at velocities ranging from 1-8.4 km/s. The LGG is used extensively for research in aerospace to analyze the effects of high speed impacts on materials. Ideally the measurement should be made close to the centre of the impact to minimise corruption of the data from edge effects and survive the impact. A further requirement is that it should operate at a stand-off distance of ~ 8cm. For these reasons we chose to develop a pseudo con-focal intensity sensor, which demonstrated resolution comparable with conventional PVDF sensors combined with high survivability and low cost. A second sensor was developed based on 'Fibre Bragg Gratings' (FBG) which although requiring contact with the target the low weight and very small contact area had minimal effect on the dynamics of the target. The FBG was mounted either on the surface of the target or tangentially between a fixed location. The output signals from the FBG were interrogated in time by a new method. Measurements were made on composite and aluminium plates in the LGG and on low speed drop tests. The particle momentum for the drop tests was chosen to be similar to that of the particles used in the LGG

Analysis of histone post translational modifications in primary monocyte derived macrophages using reverse phase×reverse phase chromatography in conjunction with porous graphitic carbon stationary phase.

A two dimensional-liquid chromatography (2D-LC) based approach was developed for the identification and quantification of histone post translational modifications in conjunction with mass spectrometry analysis. Using a bottom-up strategy, offline 2D-LC was developed using reverse phase chromatography. A porous graphitic carbon stationary phase in the first dimension and a C18 stationary phase in the second dimension interfaced with mass spectrometry was used to analyse global levels of histone post translational modifications in human primary monocyte-derived macrophages. The results demonstrated that 84 different histone peptide proteoforms, with modifications at 18 different sites including combinatorial marks were identified, representing an increase in the identification of histone peptides by 65% and 51% compared to two different 1D-LC approaches on the same mass spectrometer. The use of the porous graphitic stationary phase in the first dimension resulted in efficient separation of histone peptides across the gradient, with good resolution and is orthogonal to the online C18 reverse phase chromatography. Overall, more histone peptides were identified using the 2D-LC approach compared to conventional 1D-LC approaches. In addition, a bioinformatic pipeline was developed in-house to enable the high throughput efficient and accurate quantification of fractionated histone peptides. The automation of a section of the downstream analysis pipeline increased the throughput of the 2D-LC-MS/MS approach for the quantification of histone post translational modifications

Comparison of data acquisition methods for the identification and quantification of histone post-translational modifications on a Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer.

RATIONALE: Histone PTMs play key roles in regulating eukaryotic gene expression. Mass spectrometry (MS) has emerged as a powerful method to characterize and quantify histone PTMs as it allows unbiased identification and quantification of multiple histone PTMs including combinations of the modifications present. METHODS: In this study we compared a range of data acquisition methods for the identification and quantification of the histone PTMs using a Q Exactive HF Orbitrap. We compared three different data-dependent analysis (DDA) methods with MS2 resolutions of 120K, 60K, 30K. We also compared a range of data-independent analysis (DIA) methods using MS2 isolation windows of 20 m/z and DIAvw to identify and quantify histone PTMs in Chinese Hamster Ovary (CHO) cells. RESULTS: The increased number of MS2 scans afforded by the lower resolution methods resulted in a higher number of queries, peptide sequence matches (PSMs) and a higher number of peptide proteoforms with a Mascot Ion score greater than 46. No difference in the proportion of peptide proteoforms with Delta scores >17 was observed. Comparing the data acquisition methods increased repeatability in terms of lower CVs afforded by DIA MS1 60K MS2 30K 20m/z isolation windows was observed. CONCLUSION: We observed that DIA which offers advantages in flexibility and identification of isobaric peptide proteoforms performs as well as DDA in the analysis of histone PTMs. We were able to identify 71 modified histone peptides for histone H3 and H4 and quantified 64 across each of the different acquisition methods

Dephasing of Electrons on Helium by Collisions with Gas Atoms

The damping of quantum effects in the transport properties of electrons deposited on a surface of liquid helium is studied. It is found that due to vertical motion of the helium vapour atoms the interference of paths of duration $t$ is damped by a factor $\exp - (t/\tau_v)^3$. An expression is derived for the weak-localization lineshape in the case that damping occurs by a combination of processes with this type of cubic exponential damping and processes with a simple exponential damping factor.Comment: 7 pages, 2 figures, Revte

The Hypervelocity Impact Facility at the University of Kent: Recent Upgrades and Specialized Capabilities.

Impact events are ubiquitous across the entire Solar System; craters are observed from Mercury to distant Pluto. This process has been occurring since the Solar System formed and is still occurring today. During such events, which typically occur at speeds measured in kilometers per second, extreme pressures and elevated temperatures are created. In order to understand the physical processes that occur under such conditions, we have been using a two-stage light gas gun to recreate hypervelocity impacts on a range of targets that are representative (in both composition and physical condition) of the surfaces of all objects within the Solar System. Within this paper we describe the advances we have made in light-gas gun technology, specifically focusing on the University of Kent’s light gas-gun, over the past 30 years which have led to significant advancements in Planetary Science and the general field of shock physics

Conformal p-branes as a Source of Structure in Spacetime

We discuss a model of a conformal p-brane interacting with the world volume metric and connection. The purpose of the model is to suggest a mechanism by which gravity coupled to p-branes leads to the formation of structure rather than homogeneity in spacetime. Furthermore, we show that the formation of structure is accompanied by the appearance of a multivalued cosmological constant, i.e., one which may take on different values in different domains, or cells, of spacetime. The above results apply to a broad class of non linear gravitational lagrangians as long as metric and connection on the p-brane manifold are treated as independent variables.Comment: 10 pages, ReVTeX, no figure